FR3110178B1 - Procédé de génotypage adapté au traitement simultané d’un grand nombre de patients - Google Patents

Procédé de génotypage adapté au traitement simultané d’un grand nombre de patients Download PDF

Info

Publication number
FR3110178B1
FR3110178B1 FR2004917A FR2004917A FR3110178B1 FR 3110178 B1 FR3110178 B1 FR 3110178B1 FR 2004917 A FR2004917 A FR 2004917A FR 2004917 A FR2004917 A FR 2004917A FR 3110178 B1 FR3110178 B1 FR 3110178B1
Authority
FR
France
Prior art keywords
large number
patients
process suitable
simultaneous treatment
genotyping process
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Active
Application number
FR2004917A
Other languages
English (en)
Other versions
FR3110178A1 (fr
Inventor
Pascal Herve
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Bionobis SARL
Original Assignee
Bionobis SARL
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Bionobis SARL filed Critical Bionobis SARL
Priority to FR2004917A priority Critical patent/FR3110178B1/fr
Priority to EP21732473.0A priority patent/EP4153778A1/fr
Priority to PCT/FR2021/050862 priority patent/WO2021234269A1/fr
Publication of FR3110178A1 publication Critical patent/FR3110178A1/fr
Application granted granted Critical
Publication of FR3110178B1 publication Critical patent/FR3110178B1/fr
Active legal-status Critical Current
Anticipated expiration legal-status Critical

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844—Nucleic acid amplification reactions
    • C12Q1/686—Polymerase chain reaction [PCR]

Landscapes

  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Molecular Biology (AREA)
  • Biochemistry (AREA)
  • Microbiology (AREA)
  • Biotechnology (AREA)
  • Biophysics (AREA)
  • Analytical Chemistry (AREA)
  • Physics & Mathematics (AREA)
  • Immunology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Peptides Or Proteins (AREA)

Abstract

L’invention a trait à un procédé de génotypage permettant de traiter simultanément un grand nombre de patients. Plus particulièrement, elle concerne un procédé de génotypage comportant une première étape d’amplification par PCR indexée puis une seconde étape de séquençage à haut débit, ce procédé est simple et moins coûteux que les procédés de l’état de la technique.
FR2004917A 2020-05-18 2020-05-18 Procédé de génotypage adapté au traitement simultané d’un grand nombre de patients Active FR3110178B1 (fr)

Priority Applications (3)

Application Number Priority Date Filing Date Title
FR2004917A FR3110178B1 (fr) 2020-05-18 2020-05-18 Procédé de génotypage adapté au traitement simultané d’un grand nombre de patients
EP21732473.0A EP4153778A1 (fr) 2020-05-18 2021-05-18 Procede de genotypage adapte au traitement simultane d'un grand nombre de patients
PCT/FR2021/050862 WO2021234269A1 (fr) 2020-05-18 2021-05-18 Procede de genotypage adapte au traitement simultane d'un grand nombre de patients

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
FR2004917 2020-05-18
FR2004917A FR3110178B1 (fr) 2020-05-18 2020-05-18 Procédé de génotypage adapté au traitement simultané d’un grand nombre de patients

Publications (2)

Publication Number Publication Date
FR3110178A1 FR3110178A1 (fr) 2021-11-19
FR3110178B1 true FR3110178B1 (fr) 2022-05-27

Family

ID=73013484

Family Applications (1)

Application Number Title Priority Date Filing Date
FR2004917A Active FR3110178B1 (fr) 2020-05-18 2020-05-18 Procédé de génotypage adapté au traitement simultané d’un grand nombre de patients

Country Status (3)

Country Link
EP (1) EP4153778A1 (fr)
FR (1) FR3110178B1 (fr)
WO (1) WO2021234269A1 (fr)

Family Cites Families (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CA2803940C (fr) 2010-06-30 2019-07-02 Bgi Shenzhen Co., Limited Nouvelle methode de sequencage par pcr et son utilisation dans le genotypage hla
KR101672240B1 (ko) * 2015-12-30 2016-11-07 대한민국 인간 객체 염색체 상의 str 분석방법 및 이를 이용한 분석 키트

Also Published As

Publication number Publication date
WO2021234269A1 (fr) 2021-11-25
EP4153778A1 (fr) 2023-03-29
FR3110178A1 (fr) 2021-11-19

Similar Documents

Publication Publication Date Title
US11584964B2 (en) Method for accurate sequencing of DNA
Van Neste et al. My-Forensic-Loci-queries (MyFLq) framework for analysis of forensic STR data generated by massive parallel sequencing
Parson et al. Massively parallel sequencing of complete mitochondrial genomes from hair shaft samples
EP3055431B1 (fr) Séquençages d'adn synthétique à lectures longues
EP2971069B1 (fr) Procédés et systèmes pour aligner des éléments d'adn répétitifs
US20240043832A1 (en) Method for generating extended sequence reads
US20190376137A1 (en) Safe sequencing system
US20100035249A1 (en) Rna sequencing and analysis using solid support
EP2272976A1 (fr) Procédé de différentiation de brins de polynucléotide
FR3110178B1 (fr) Procédé de génotypage adapté au traitement simultané d’un grand nombre de patients
JP2006523451A (ja) ポリヌクレオチドの特性決定方法
CN114096682A (zh) 通过合成方法的快进测序
Profaizer et al. Report on the effects of fragment size, indexing, and read length on HLA sequencing on the Illumina MiSeq
JP7280191B2 (ja) 複数の標的を増幅するためのダイマー回避マルチプレックスポリメラーゼ連鎖反応
FR3106596B1 (fr) Procede de genotypage hla simple et rapide
FR3100820B1 (fr) Procédé de génotypage adapté au traitement d’un grand nombre d’échantillons, notamment en cas de polymorphisme élevé
Elvidge et al. Development and evaluation of real competitive PCR for high-throughput quantitative applications
WO2018232597A1 (fr) Composition utilisée pour amplification pcr quantitative et son application
Bürckert et al. High-throughput sequencing of murine immunoglobulin heavy chain repertoires using single side unique molecular identifiers on an Ion Torrent PGM
Kieleczawa Controlled heat-denaturation of DNA plasmids
KR20200126561A (ko) 디옥시우리딘을 이용한 차세대 염기서열 분석을 위한 라이브러리 제조 방법
WO2015199939A1 (fr) Compositions et procedes pour l'amplification et la determination de sequences d'acides nucleiques
CN118116466A (zh) 嵌合体序列的鉴定方法及***
US9637779B2 (en) Antisense transcriptomes of cells
GB202313364D0 (en) Unbiased and simultaneous amplification method for preparing a double-stranded dna library from a sample of more than one type of nucleic acid

Legal Events

Date Code Title Description
PLFP Fee payment

Year of fee payment: 2

PLSC Publication of the preliminary search report

Effective date: 20211119

PLFP Fee payment

Year of fee payment: 3

PLFP Fee payment

Year of fee payment: 4

PLFP Fee payment

Year of fee payment: 5