FR3083246B1 - Modeles cellulaires et tissulaires comprenant des fibroblastes de la jonction dermo-hypodermique et ses applications - Google Patents
Modeles cellulaires et tissulaires comprenant des fibroblastes de la jonction dermo-hypodermique et ses applications Download PDFInfo
- Publication number
- FR3083246B1 FR3083246B1 FR1855987A FR1855987A FR3083246B1 FR 3083246 B1 FR3083246 B1 FR 3083246B1 FR 1855987 A FR1855987 A FR 1855987A FR 1855987 A FR1855987 A FR 1855987A FR 3083246 B1 FR3083246 B1 FR 3083246B1
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- dermo
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- 210000002950 fibroblast Anatomy 0.000 title abstract 3
- 230000001413 cellular effect Effects 0.000 title abstract 2
- 238000013334 tissue model Methods 0.000 title abstract 2
- 238000000034 method Methods 0.000 abstract 2
- 239000004480 active ingredient Substances 0.000 abstract 1
- 210000001789 adipocyte Anatomy 0.000 abstract 1
- 210000001612 chondrocyte Anatomy 0.000 abstract 1
- 238000012258 culturing Methods 0.000 abstract 1
- 230000004069 differentiation Effects 0.000 abstract 1
- 238000000338 in vitro Methods 0.000 abstract 1
- 210000000963 osteoblast Anatomy 0.000 abstract 1
- 230000001737 promoting effect Effects 0.000 abstract 1
- 238000012216 screening Methods 0.000 abstract 1
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- C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
- C12N5/06—Animal cells or tissues; Human cells or tissues
- C12N5/0602—Vertebrate cells
- C12N5/0652—Cells of skeletal and connective tissues; Mesenchyme
- C12N5/0656—Adult fibroblasts
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- C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
- C12N5/06—Animal cells or tissues; Human cells or tissues
- C12N5/0602—Vertebrate cells
- C12N5/0625—Epidermal cells, skin cells; Cells of the oral mucosa
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- C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
- C12N5/06—Animal cells or tissues; Human cells or tissues
- C12N5/0602—Vertebrate cells
- C12N5/0652—Cells of skeletal and connective tissues; Mesenchyme
- C12N5/0653—Adipocytes; Adipose tissue
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- C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
- C12N5/06—Animal cells or tissues; Human cells or tissues
- C12N5/0602—Vertebrate cells
- C12N5/0652—Cells of skeletal and connective tissues; Mesenchyme
- C12N5/0654—Osteocytes, Osteoblasts, Odontocytes; Bones, Teeth
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- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
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- C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
- C12N5/06—Animal cells or tissues; Human cells or tissues
- C12N5/0602—Vertebrate cells
- C12N5/0652—Cells of skeletal and connective tissues; Mesenchyme
- C12N5/0655—Chondrocytes; Cartilage
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- C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
- C12N5/06—Animal cells or tissues; Human cells or tissues
- C12N5/0697—Artificial constructs associating cells of different lineages, e.g. tissue equivalents
- C12N5/0698—Skin equivalents
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- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6876—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes
- C12Q1/6881—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for tissue or cell typing, e.g. human leukocyte antigen [HLA] probes
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- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
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- C12N2503/00—Use of cells in diagnostics
- C12N2503/04—Screening or testing on artificial tissues
- C12N2503/06—Screening or testing on artificial skin
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- C12N2506/00—Differentiation of animal cells from one lineage to another; Differentiation of pluripotent cells
- C12N2506/13—Differentiation of animal cells from one lineage to another; Differentiation of pluripotent cells from connective tissue cells, from mesenchymal cells
- C12N2506/1307—Differentiation of animal cells from one lineage to another; Differentiation of pluripotent cells from connective tissue cells, from mesenchymal cells from adult fibroblasts
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- C12N2533/00—Supports or coatings for cell culture, characterised by material
- C12N2533/50—Proteins
- C12N2533/54—Collagen; Gelatin
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- C12N2535/00—Supports or coatings for cell culture characterised by topography
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- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q2600/00—Oligonucleotides characterized by their use
- C12Q2600/158—Expression markers
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- Health & Medical Sciences (AREA)
- Engineering & Computer Science (AREA)
- Life Sciences & Earth Sciences (AREA)
- Biomedical Technology (AREA)
- Chemical & Material Sciences (AREA)
- Organic Chemistry (AREA)
- Zoology (AREA)
- Wood Science & Technology (AREA)
- Biotechnology (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Genetics & Genomics (AREA)
- General Engineering & Computer Science (AREA)
- Microbiology (AREA)
- Biochemistry (AREA)
- Cell Biology (AREA)
- General Health & Medical Sciences (AREA)
- Rheumatology (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Analytical Chemistry (AREA)
- Immunology (AREA)
- Physics & Mathematics (AREA)
- Biophysics (AREA)
- Molecular Biology (AREA)
- Dermatology (AREA)
- Orthopedic Medicine & Surgery (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Investigating Or Analysing Biological Materials (AREA)
Abstract
La présente invention a pour objet un procédé de préparation in vitro de modèles cellulaire et tissulaire comprenant au moins une étape de culture de fibroblastes de la jonction dermo-hypodermique ; les modèles obtenus ainsi que leurs mises en œuvre dans des procédés de criblage d'actifs favorisant la différenciation de fibroblastes de la jonction dermo-hypodermique en cellules adipocytes, ostéoblastes et/ou chondroblastes.
Priority Applications (6)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
FR1855987A FR3083246B1 (fr) | 2018-06-29 | 2018-06-29 | Modeles cellulaires et tissulaires comprenant des fibroblastes de la jonction dermo-hypodermique et ses applications |
BR112020026219-6A BR112020026219A2 (pt) | 2018-06-29 | 2019-07-01 | Processos in vitro para a preparação de um modelo celular e para a preparação de um modelo tecidual, modelo celular in vitro, modelo tecidual in vitro, uso de modelos celulares ou teciduais in vitro e processo para a triagem de agentes ativos |
CN201980042828.4A CN112352044A (zh) | 2018-06-29 | 2019-07-01 | 包含真皮-皮下接合的纤维母细胞的细胞和组织模型及其应用 |
US16/973,916 US20210277359A1 (en) | 2018-06-29 | 2019-07-01 | Cell and tissue models comprising fibroblasts of the dermo-hypodermic junction and applications thereof |
PCT/EP2019/067638 WO2020002711A1 (fr) | 2018-06-29 | 2019-07-01 | Modèles cellulaires et tissulaires comprenant des fibroblastes de la jonction dermo-hypodermique et leurs applications |
EP19734096.1A EP3814483A1 (fr) | 2018-06-29 | 2019-07-01 | Modèles cellulaires et tissulaires comprenant des fibroblastes de la jonction dermo-hypodermique et leurs applications |
Applications Claiming Priority (2)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
FR1855987 | 2018-06-29 | ||
FR1855987A FR3083246B1 (fr) | 2018-06-29 | 2018-06-29 | Modeles cellulaires et tissulaires comprenant des fibroblastes de la jonction dermo-hypodermique et ses applications |
Publications (2)
Publication Number | Publication Date |
---|---|
FR3083246A1 FR3083246A1 (fr) | 2020-01-03 |
FR3083246B1 true FR3083246B1 (fr) | 2023-12-08 |
Family
ID=65443895
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
FR1855987A Active FR3083246B1 (fr) | 2018-06-29 | 2018-06-29 | Modeles cellulaires et tissulaires comprenant des fibroblastes de la jonction dermo-hypodermique et ses applications |
Country Status (6)
Country | Link |
---|---|
US (1) | US20210277359A1 (fr) |
EP (1) | EP3814483A1 (fr) |
CN (1) | CN112352044A (fr) |
BR (1) | BR112020026219A2 (fr) |
FR (1) | FR3083246B1 (fr) |
WO (1) | WO2020002711A1 (fr) |
Family Cites Families (9)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
BR9815713A (pt) * | 1997-02-20 | 2002-11-05 | Gerigene Medical Corp | Método para aumento e reparo de defeitos de tecido dérmico, subcutâneo e das cordas vocais |
WO1999043270A1 (fr) * | 1998-02-24 | 1999-09-02 | Gerigene Medical Corporation | Augmentation des tissus dermiques, sous-cutanes et des cordes vocales et reparation de leurs defauts |
DK1290145T3 (da) * | 2000-05-31 | 2006-03-13 | Fraunhofer Ges Forschung | Tredimensional hudmodel |
FR2950074B1 (fr) * | 2009-09-16 | 2017-10-06 | Oreal | Equivalent de peau in vitro et procede de preparation |
CN102399745A (zh) * | 2011-11-22 | 2012-04-04 | 浙江大学 | 一种软骨干细胞分离培养方法 |
WO2013137664A1 (fr) * | 2012-03-15 | 2013-09-19 | 주식회사 엘앤씨바이오 | Implant de type tissu dermique acellulaire |
FR2993282B1 (fr) * | 2012-07-13 | 2017-11-10 | Expanscience Lab | Procede d'identification de marqueurs moleculaires de la peau d'enfant |
WO2014160008A1 (fr) * | 2013-03-14 | 2014-10-02 | Musculoskeletal Transplant Foundation | Allogreffes de réparation de tissu mou et procédé pour préparer celles-ci |
FR3071512B1 (fr) * | 2017-09-28 | 2022-02-25 | Oreal | Signatures moleculaires de trois sous-populations de fibroblastes dermiques et equivalent de derme comprenant une de ces sous-populations |
-
2018
- 2018-06-29 FR FR1855987A patent/FR3083246B1/fr active Active
-
2019
- 2019-07-01 EP EP19734096.1A patent/EP3814483A1/fr active Pending
- 2019-07-01 US US16/973,916 patent/US20210277359A1/en active Pending
- 2019-07-01 BR BR112020026219-6A patent/BR112020026219A2/pt unknown
- 2019-07-01 CN CN201980042828.4A patent/CN112352044A/zh active Pending
- 2019-07-01 WO PCT/EP2019/067638 patent/WO2020002711A1/fr active Application Filing
Also Published As
Publication number | Publication date |
---|---|
WO2020002711A1 (fr) | 2020-01-02 |
EP3814483A1 (fr) | 2021-05-05 |
BR112020026219A2 (pt) | 2021-04-06 |
FR3083246A1 (fr) | 2020-01-03 |
CN112352044A (zh) | 2021-02-09 |
US20210277359A1 (en) | 2021-09-09 |
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