FR3052170B1 - Production de frambinone par un microorganisme fongique recombinant - Google Patents

Production de frambinone par un microorganisme fongique recombinant Download PDF

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Publication number
FR3052170B1
FR3052170B1 FR1655089A FR1655089A FR3052170B1 FR 3052170 B1 FR3052170 B1 FR 3052170B1 FR 1655089 A FR1655089 A FR 1655089A FR 1655089 A FR1655089 A FR 1655089A FR 3052170 B1 FR3052170 B1 FR 3052170B1
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France
Prior art keywords
frambinone
production
fungal microorganism
recombinant fungal
microorganism
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Active
Application number
FR1655089A
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English (en)
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FR3052170A1 (fr
Inventor
Alexander Matthias Farwick
Thomas Desfougeres
Georges Pignede
Anais Roussel
Isabelle Mouly
Brieuc Morvan
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Lesaffre et Cie SA
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Lesaffre et Cie SA
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Publication date
Application filed by Lesaffre et Cie SA filed Critical Lesaffre et Cie SA
Priority to FR1655089A priority Critical patent/FR3052170B1/fr
Priority to US16/302,527 priority patent/US10793880B2/en
Priority to EP17732985.1A priority patent/EP3464556A1/fr
Priority to PCT/FR2017/051407 priority patent/WO2017207950A1/fr
Publication of FR3052170A1 publication Critical patent/FR3052170A1/fr
Priority to US17/004,863 priority patent/US11345936B2/en
Application granted granted Critical
Publication of FR3052170B1 publication Critical patent/FR3052170B1/fr
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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P7/00—Preparation of oxygen-containing organic compounds
    • C12P7/24—Preparation of oxygen-containing organic compounds containing a carbonyl group
    • C12P7/26—Ketones
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07C—ACYCLIC OR CARBOCYCLIC COMPOUNDS
    • C07C49/00—Ketones; Ketenes; Dimeric ketenes; Ketonic chelates
    • C07C49/20—Unsaturated compounds containing keto groups bound to acyclic carbon atoms
    • C07C49/24—Unsaturated compounds containing keto groups bound to acyclic carbon atoms containing hydroxy groups
    • C07C49/245—Unsaturated compounds containing keto groups bound to acyclic carbon atoms containing hydroxy groups containing six-membered aromatic rings
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
    • C12N1/14—Fungi; Culture media therefor
    • C12N1/16—Yeasts; Culture media therefor
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
    • C12N1/14—Fungi; Culture media therefor
    • C12N1/16—Yeasts; Culture media therefor
    • C12N1/18—Baker's yeast; Brewer's yeast
    • C12N1/185—Saccharomyces isolates
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C12N15/52—Genes encoding for enzymes or proenzymes
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/0004—Oxidoreductases (1.)
    • C12N9/001—Oxidoreductases (1.) acting on the CH-CH group of donors (1.3)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/0004—Oxidoreductases (1.)
    • C12N9/0071—Oxidoreductases (1.) acting on paired donors with incorporation of molecular oxygen (1.14)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/10—Transferases (2.)
    • C12N9/1025—Acyltransferases (2.3)
    • C12N9/1029—Acyltransferases (2.3) transferring groups other than amino-acyl groups (2.3.1)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P7/00—Preparation of oxygen-containing organic compounds
    • C12P7/02—Preparation of oxygen-containing organic compounds containing a hydroxy group
    • C12P7/22—Preparation of oxygen-containing organic compounds containing a hydroxy group aromatic
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12R—INDEXING SCHEME ASSOCIATED WITH SUBCLASSES C12C - C12Q, RELATING TO MICROORGANISMS
    • C12R2001/00—Microorganisms ; Processes using microorganisms
    • C12R2001/645—Fungi ; Processes using fungi
    • C12R2001/85—Saccharomyces
    • C12R2001/865—Saccharomyces cerevisiae
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y401/00—Carbon-carbon lyases (4.1)
    • C12Y401/01—Carboxy-lyases (4.1.1)
    • C12Y401/0108—4-Hydroxyphenylpyruvate decarboxylase (4.1.1.80)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y403/00—Carbon-nitrogen lyases (4.3)
    • C12Y403/01—Ammonia-lyases (4.3.1)
    • C12Y403/01023—Tyrosine ammonia-lyase (4.3.1.23)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y403/00—Carbon-nitrogen lyases (4.3)
    • C12Y403/01—Ammonia-lyases (4.3.1)
    • C12Y403/01024—Phenylalanine ammonia-lyase (4.3.1.24)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y602/00—Ligases forming carbon-sulfur bonds (6.2)
    • C12Y602/01—Acid-Thiol Ligases (6.2.1)
    • C12Y602/01012—4-Coumarate-CoA ligase (6.2.1.12)

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  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Organic Chemistry (AREA)
  • Engineering & Computer Science (AREA)
  • Genetics & Genomics (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • Biomedical Technology (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Microbiology (AREA)
  • Molecular Biology (AREA)
  • Medicinal Chemistry (AREA)
  • Mycology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • General Chemical & Material Sciences (AREA)
  • Botany (AREA)
  • Tropical Medicine & Parasitology (AREA)
  • Virology (AREA)
  • Plant Pathology (AREA)
  • Biophysics (AREA)
  • Physics & Mathematics (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)

Abstract

La présente invention concerne un microorganisme fongique modifié génétiquement pour la production de frambinone, ledit microorganisme présentant les caractéristiques suivantes : - la capacité à produire de la frambinone à partir de tyrosine ; et - une capacité faible ou une incapacité à dégrader la tyrosine en tyrosol, en p-hydroxyphényl acétaldéhyde et/ou en p-hydroxyphényl acétate, et son utilisation pour la production de frambinone.
FR1655089A 2016-06-03 2016-06-03 Production de frambinone par un microorganisme fongique recombinant Active FR3052170B1 (fr)

Priority Applications (5)

Application Number Priority Date Filing Date Title
FR1655089A FR3052170B1 (fr) 2016-06-03 2016-06-03 Production de frambinone par un microorganisme fongique recombinant
US16/302,527 US10793880B2 (en) 2016-06-03 2017-06-02 Production of frambinone by a recombinant fungal microorganism
EP17732985.1A EP3464556A1 (fr) 2016-06-03 2017-06-02 Production de frambinone par un microorganisme fongique recombinant
PCT/FR2017/051407 WO2017207950A1 (fr) 2016-06-03 2017-06-02 Production de frambinone par un microorganisme fongique recombinant
US17/004,863 US11345936B2 (en) 2016-06-03 2020-08-27 Production of frambinone by a recombinant fungal microorganism

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
FR1655089A FR3052170B1 (fr) 2016-06-03 2016-06-03 Production de frambinone par un microorganisme fongique recombinant

Publications (2)

Publication Number Publication Date
FR3052170A1 FR3052170A1 (fr) 2017-12-08
FR3052170B1 true FR3052170B1 (fr) 2021-01-22

Family

ID=57045072

Family Applications (1)

Application Number Title Priority Date Filing Date
FR1655089A Active FR3052170B1 (fr) 2016-06-03 2016-06-03 Production de frambinone par un microorganisme fongique recombinant

Country Status (4)

Country Link
US (2) US10793880B2 (fr)
EP (1) EP3464556A1 (fr)
FR (1) FR3052170B1 (fr)
WO (1) WO2017207950A1 (fr)

Families Citing this family (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2019097049A1 (fr) * 2017-11-20 2019-05-23 Axxence Holding B.V. Production d'un composé aromatique dans une cellule hôte
CN108753852B (zh) * 2018-06-22 2021-10-26 江南大学 一种生物法制备覆盆子酮的方法
CN111748534B (zh) * 2020-08-10 2022-05-10 天津中医药大学 一种SmC4H蛋白及其构建与表达方法
FR3122882A1 (fr) * 2021-05-12 2022-11-18 Bgene Genetics Biosynthèse de composés phénylpropanoïdes

Family Cites Families (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US6521748B2 (en) 1999-08-06 2003-02-18 E. I. Du Pont De Nemours And Company Polynucleotide encoding a mutant Rhodotorula glutinis tyrosine ammonia lyase polypeptide
GB2416770A (en) 2004-07-28 2006-02-08 Danisco Synthesis of benzalacetone/raspberry ketone by chalcone synthase
GB2416769A (en) * 2004-07-28 2006-02-08 Danisco Biosynthesis of raspberry ketone
EP2957629A1 (fr) * 2014-06-18 2015-12-23 Rhodia Opérations Production améliorée de vanilloïdes par fermentation

Also Published As

Publication number Publication date
US20190309330A1 (en) 2019-10-10
US11345936B2 (en) 2022-05-31
US20200392545A1 (en) 2020-12-17
FR3052170A1 (fr) 2017-12-08
EP3464556A1 (fr) 2019-04-10
US10793880B2 (en) 2020-10-06
WO2017207950A1 (fr) 2017-12-07

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