FR2948665B1 - Procede pour la purification de proteines de la coagulation a domaine gla actives - Google Patents

Procede pour la purification de proteines de la coagulation a domaine gla actives

Info

Publication number
FR2948665B1
FR2948665B1 FR0955413A FR0955413A FR2948665B1 FR 2948665 B1 FR2948665 B1 FR 2948665B1 FR 0955413 A FR0955413 A FR 0955413A FR 0955413 A FR0955413 A FR 0955413A FR 2948665 B1 FR2948665 B1 FR 2948665B1
Authority
FR
France
Prior art keywords
domaine
purification
coagulation proteins
active gla
gla
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired - Fee Related
Application number
FR0955413A
Other languages
English (en)
Other versions
FR2948665A1 (fr
Inventor
Gerald Perret
Nicolas Bihoreau
Laurent Siret
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
LFB SA
Original Assignee
LFB Biotechnologies SAS
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Priority to FR0955413A priority Critical patent/FR2948665B1/fr
Application filed by LFB Biotechnologies SAS filed Critical LFB Biotechnologies SAS
Priority to EP10762958A priority patent/EP2459719A1/fr
Priority to CN201080043798.8A priority patent/CN102666857B/zh
Priority to KR1020127005553A priority patent/KR20120105419A/ko
Priority to PCT/FR2010/051629 priority patent/WO2011012831A1/fr
Priority to AU2010277421A priority patent/AU2010277421B2/en
Priority to CA2771850A priority patent/CA2771850C/fr
Priority to US13/392,989 priority patent/US9212365B2/en
Publication of FR2948665A1 publication Critical patent/FR2948665A1/fr
Application granted granted Critical
Publication of FR2948665B1 publication Critical patent/FR2948665B1/fr
Priority to IL218261A priority patent/IL218261A0/en
Priority to US14/936,896 priority patent/US9850477B2/en
Expired - Fee Related legal-status Critical Current
Anticipated expiration legal-status Critical

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/48—Hydrolases (3) acting on peptide bonds (3.4)
    • C12N9/50—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25)
    • C12N9/64—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from animal tissue
    • C12N9/6421—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from animal tissue from mammals
    • C12N9/6424—Serine endopeptidases (3.4.21)
    • C12N9/6432—Coagulation factor Xa (3.4.21.6)
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
    • C07K14/745—Blood coagulation or fibrinolysis factors
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C12N15/115—Aptamers, i.e. nucleic acids binding a target molecule specifically and with high affinity without hybridising therewith ; Nucleic acids binding to non-nucleic acids, e.g. aptamers
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/48—Hydrolases (3) acting on peptide bonds (3.4)
    • C12N9/50—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25)
    • C12N9/64—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from animal tissue
    • C12N9/6421—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from animal tissue from mammals
    • C12N9/6424—Serine endopeptidases (3.4.21)
    • C12N9/6437—Coagulation factor VIIa (3.4.21.21)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/48—Hydrolases (3) acting on peptide bonds (3.4)
    • C12N9/50—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25)
    • C12N9/64—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from animal tissue
    • C12N9/6421—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from animal tissue from mammals
    • C12N9/6424—Serine endopeptidases (3.4.21)
    • C12N9/644—Coagulation factor IXa (3.4.21.22)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2310/00—Structure or type of the nucleic acid
    • C12N2310/10—Type of nucleic acid
    • C12N2310/16—Aptamers
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2310/00—Structure or type of the nucleic acid
    • C12N2310/30—Chemical structure
    • C12N2310/35—Nature of the modification
    • C12N2310/351—Conjugate
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2330/00—Production
    • C12N2330/30—Production chemically synthesised
    • C12N2330/31—Libraries, arrays
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y304/00—Hydrolases acting on peptide bonds, i.e. peptidases (3.4)
    • C12Y304/21—Serine endopeptidases (3.4.21)
    • C12Y304/21006—Coagulation factor Xa (3.4.21.6)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y304/00—Hydrolases acting on peptide bonds, i.e. peptidases (3.4)
    • C12Y304/21—Serine endopeptidases (3.4.21)
    • C12Y304/21021—Coagulation factor VIIa (3.4.21.21)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y304/00—Hydrolases acting on peptide bonds, i.e. peptidases (3.4)
    • C12Y304/21—Serine endopeptidases (3.4.21)
    • C12Y304/21022—Coagulation factor IXa (3.4.21.22)
FR0955413A 2009-07-31 2009-07-31 Procede pour la purification de proteines de la coagulation a domaine gla actives Expired - Fee Related FR2948665B1 (fr)

Priority Applications (10)

Application Number Priority Date Filing Date Title
FR0955413A FR2948665B1 (fr) 2009-07-31 2009-07-31 Procede pour la purification de proteines de la coagulation a domaine gla actives
US13/392,989 US9212365B2 (en) 2009-07-31 2010-07-30 Method for purifying active GLA-domain coagulation proteins
KR1020127005553A KR20120105419A (ko) 2009-07-31 2010-07-30 활성 gla―도메인 응고 단백질 정제 방법
PCT/FR2010/051629 WO2011012831A1 (fr) 2009-07-31 2010-07-30 Procede pour la purification de proteines de la coagulation a domaine gla actives
AU2010277421A AU2010277421B2 (en) 2009-07-31 2010-07-30 Method for purifying active GLA-domain coagulation proteins
CA2771850A CA2771850C (fr) 2009-07-31 2010-07-30 Procede pour la purification de proteines de la coagulation a domaine gla actives
EP10762958A EP2459719A1 (fr) 2009-07-31 2010-07-30 Procede pour la purification de proteines de la coagulation a domaine gla actives
CN201080043798.8A CN102666857B (zh) 2009-07-31 2010-07-30 纯化活性gla结构域凝固蛋白的方法
IL218261A IL218261A0 (en) 2009-07-31 2012-02-22 Method for purifying active gla-domain coagulation proteins
US14/936,896 US9850477B2 (en) 2009-07-31 2015-11-10 Method for purifying active GLA-domain coagulation proteins

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
FR0955413A FR2948665B1 (fr) 2009-07-31 2009-07-31 Procede pour la purification de proteines de la coagulation a domaine gla actives

Publications (2)

Publication Number Publication Date
FR2948665A1 FR2948665A1 (fr) 2011-02-04
FR2948665B1 true FR2948665B1 (fr) 2011-09-23

Family

ID=41724677

Family Applications (1)

Application Number Title Priority Date Filing Date
FR0955413A Expired - Fee Related FR2948665B1 (fr) 2009-07-31 2009-07-31 Procede pour la purification de proteines de la coagulation a domaine gla actives

Country Status (9)

Country Link
US (2) US9212365B2 (fr)
EP (1) EP2459719A1 (fr)
KR (1) KR20120105419A (fr)
CN (1) CN102666857B (fr)
AU (1) AU2010277421B2 (fr)
CA (1) CA2771850C (fr)
FR (1) FR2948665B1 (fr)
IL (1) IL218261A0 (fr)
WO (1) WO2011012831A1 (fr)

Families Citing this family (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
FR3050992A1 (fr) * 2016-05-06 2017-11-10 Lab Francais Du Fractionnement Mutants du facteur x
FR3077296A1 (fr) 2018-02-01 2019-08-02 Laboratoire Francais Du Fractionnement Et Des Biotechnologies Dimeres de variants du facteur x
CN109400670A (zh) * 2018-10-30 2019-03-01 廖世奇 一种核酸适配体纯化基因工程中表达蛋白的方法及试剂盒

Family Cites Families (18)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
DE122007000007I1 (de) 1986-04-09 2007-05-16 Genzyme Corp Genetisch transformierte Tiere, die ein gewünschtes Protein in Milch absondern
DE3826792C1 (fr) * 1988-08-06 1989-07-06 Biotest Pharma Gmbh, 6072 Dreieich, De
EP1493825A3 (fr) 1990-06-11 2005-02-09 Gilead Sciences, Inc. Procédé pour la production de ligands constitués par des acides nucléiques
US5580737A (en) 1990-06-11 1996-12-03 Nexstar Pharmaceuticals, Inc. High-affinity nucleic acid ligands that discriminate between theophylline and caffeine
FR2677652B1 (fr) 1991-06-12 2005-05-27 Agronomique Inst Nat Rech Procede de preparation d'une proteine d'interet dans le lait d'un animal transgenique, produit obtenu et cellule eucaryote utilisee.
AU689087B2 (en) 1992-09-29 1998-03-26 Nexstar Pharmaceuticals, Inc. Nucleic acid ligands and methods for producing the same
US5880327A (en) 1994-09-21 1999-03-09 American National Red Cross Transgenic mammals expressing human coagulation factor VIII
SE0000675D0 (sv) * 2000-03-02 2000-03-02 Protease Ab Monoclonal antibodies
US7312325B2 (en) * 2000-09-26 2007-12-25 Duke University RNA aptamers and methods for identifying the same
IL159053A0 (en) 2001-05-25 2004-05-12 Univ Duke Modulators of pharmacological agents
US8367627B2 (en) * 2005-09-15 2013-02-05 Duke University Focused libraries, functional profiling, laser SELEX, and DESELEX
CA2661668A1 (fr) 2006-09-01 2008-03-06 Vib Vzw Compositions d'ecran solaire
WO2008038696A1 (fr) 2006-09-27 2008-04-03 National University Corporation Tokyo University Of Agriculture And Technology Procédé de dosage d'une substance cible dans un échantillon, aptamère et son procédé de fabrication
KR100896987B1 (ko) 2007-03-14 2009-05-14 한국과학기술연구원 앱타머를 이용한 표적 단백질 검출 방법 및 검출키트
FR2942231B1 (fr) * 2009-02-19 2015-03-20 Lfb Biotechnologies Acides nucleiques se liant specifiquement au facteur vii/viia humain, et utilisations
FR2942232B1 (fr) * 2009-02-19 2015-03-13 Lfb Biotechnologies Moyens pour la purification d'une proteine de la coagulation et procedes pour sa mise en oeuvre
FR2942233B1 (fr) * 2009-02-19 2015-03-13 Lfb Biotechnologies Moyens pour la purification d'une proteine du plasma sanguin, et procedes pour sa mise en oeuvre
FR2948664B1 (fr) * 2009-07-31 2013-11-01 Lfb Biotechnologies Procede pour la purification de proteines de la coagulation a domaine gla

Also Published As

Publication number Publication date
US9850477B2 (en) 2017-12-26
CA2771850C (fr) 2020-02-18
US20120271039A1 (en) 2012-10-25
CN102666857B (zh) 2017-03-08
US9212365B2 (en) 2015-12-15
WO2011012831A1 (fr) 2011-02-03
US20160138001A1 (en) 2016-05-19
KR20120105419A (ko) 2012-09-25
AU2010277421A1 (en) 2012-03-15
IL218261A0 (en) 2012-04-30
FR2948665A1 (fr) 2011-02-04
CN102666857A (zh) 2012-09-12
CA2771850A1 (fr) 2011-02-03
EP2459719A1 (fr) 2012-06-06
AU2010277421B2 (en) 2015-10-22

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Effective date: 20130827

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