FR2833017B1 - METHODS FOR SCREENING MOLECULES MODULATING GENE EXPRESSION, CELL LINES, PREPARATION AND USES - Google Patents

METHODS FOR SCREENING MOLECULES MODULATING GENE EXPRESSION, CELL LINES, PREPARATION AND USES

Info

Publication number
FR2833017B1
FR2833017B1 FR0115581A FR0115581A FR2833017B1 FR 2833017 B1 FR2833017 B1 FR 2833017B1 FR 0115581 A FR0115581 A FR 0115581A FR 0115581 A FR0115581 A FR 0115581A FR 2833017 B1 FR2833017 B1 FR 2833017B1
Authority
FR
France
Prior art keywords
preparation
methods
cell lines
gene expression
modulating gene
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired - Fee Related
Application number
FR0115581A
Other languages
French (fr)
Other versions
FR2833017A1 (en
Inventor
Clarence Deffaud
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Individual
Original Assignee
Individual
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Individual filed Critical Individual
Priority to FR0115581A priority Critical patent/FR2833017B1/en
Priority to AU2002365698A priority patent/AU2002365698A1/en
Priority to PCT/FR2002/004124 priority patent/WO2003048370A1/en
Publication of FR2833017A1 publication Critical patent/FR2833017A1/en
Application granted granted Critical
Publication of FR2833017B1 publication Critical patent/FR2833017B1/en
Anticipated expiration legal-status Critical
Expired - Fee Related legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1034—Isolating an individual clone by screening libraries
    • C12N15/1086—Preparation or screening of expression libraries, e.g. reporter assays
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/85—Vectors or expression systems specially adapted for eukaryotic hosts for animal cells
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2840/00—Vectors comprising a special translation-regulating system
    • C12N2840/20—Vectors comprising a special translation-regulating system translation of more than one cistron
    • C12N2840/203—Vectors comprising a special translation-regulating system translation of more than one cistron having an IRES
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2840/00—Vectors comprising a special translation-regulating system
    • C12N2840/20—Vectors comprising a special translation-regulating system translation of more than one cistron
    • C12N2840/203—Vectors comprising a special translation-regulating system translation of more than one cistron having an IRES
    • C12N2840/206—Vectors comprising a special translation-regulating system translation of more than one cistron having an IRES having multiple IRES

Landscapes

  • Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Chemical & Material Sciences (AREA)
  • Biomedical Technology (AREA)
  • Organic Chemistry (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Microbiology (AREA)
  • Plant Pathology (AREA)
  • Molecular Biology (AREA)
  • Physics & Mathematics (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Biophysics (AREA)
  • Bioinformatics & Computational Biology (AREA)
  • Crystallography & Structural Chemistry (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
FR0115581A 2001-12-03 2001-12-03 METHODS FOR SCREENING MOLECULES MODULATING GENE EXPRESSION, CELL LINES, PREPARATION AND USES Expired - Fee Related FR2833017B1 (en)

Priority Applications (3)

Application Number Priority Date Filing Date Title
FR0115581A FR2833017B1 (en) 2001-12-03 2001-12-03 METHODS FOR SCREENING MOLECULES MODULATING GENE EXPRESSION, CELL LINES, PREPARATION AND USES
AU2002365698A AU2002365698A1 (en) 2001-12-03 2002-12-02 Method for screening molecules modulating gene expression, cell lines, preparation and uses
PCT/FR2002/004124 WO2003048370A1 (en) 2001-12-03 2002-12-02 Method for screening molecules modulating gene expression, cell lines, preparation and uses

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
FR0115581A FR2833017B1 (en) 2001-12-03 2001-12-03 METHODS FOR SCREENING MOLECULES MODULATING GENE EXPRESSION, CELL LINES, PREPARATION AND USES

Publications (2)

Publication Number Publication Date
FR2833017A1 FR2833017A1 (en) 2003-06-06
FR2833017B1 true FR2833017B1 (en) 2005-02-04

Family

ID=8870031

Family Applications (1)

Application Number Title Priority Date Filing Date
FR0115581A Expired - Fee Related FR2833017B1 (en) 2001-12-03 2001-12-03 METHODS FOR SCREENING MOLECULES MODULATING GENE EXPRESSION, CELL LINES, PREPARATION AND USES

Country Status (3)

Country Link
AU (1) AU2002365698A1 (en)
FR (1) FR2833017B1 (en)
WO (1) WO2003048370A1 (en)

Family Cites Families (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5733726A (en) * 1995-06-07 1998-03-31 Emory University Cytotoxicity-based genetic selection system (TOXSEL)
EP0974649A1 (en) * 1998-06-22 2000-01-26 Universität Zürich Screening system
US6171821B1 (en) * 1998-07-24 2001-01-09 Apoptogen, Inc. XIAP IRES and uses thereof

Also Published As

Publication number Publication date
AU2002365698A1 (en) 2003-06-17
WO2003048370A1 (en) 2003-06-12
FR2833017A1 (en) 2003-06-06

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Legal Events

Date Code Title Description
ST Notification of lapse

Effective date: 20060831