FR2739859B1 - Polypeptide precurseur d'adn gyrase contenant une inteine - Google Patents

Polypeptide precurseur d'adn gyrase contenant une inteine

Info

Publication number
FR2739859B1
FR2739859B1 FR9512162A FR9512162A FR2739859B1 FR 2739859 B1 FR2739859 B1 FR 2739859B1 FR 9512162 A FR9512162 A FR 9512162A FR 9512162 A FR9512162 A FR 9512162A FR 2739859 B1 FR2739859 B1 FR 2739859B1
Authority
FR
France
Prior art keywords
intein
containing dna
precursor polypeptide
dna gyrase
gyrase
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired - Fee Related
Application number
FR9512162A
Other languages
English (en)
Other versions
FR2739859A1 (fr
Inventor
Halida Fsihi
Stewart Cole
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Institut Pasteur de Lille
Original Assignee
Institut Pasteur de Lille
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Institut Pasteur de Lille filed Critical Institut Pasteur de Lille
Priority to FR9512162A priority Critical patent/FR2739859B1/fr
Publication of FR2739859A1 publication Critical patent/FR2739859A1/fr
Application granted granted Critical
Publication of FR2739859B1 publication Critical patent/FR2739859B1/fr
Anticipated expiration legal-status Critical
Expired - Fee Related legal-status Critical Current

Links

Classifications

    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/90Isomerases (5.)
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09Recombinant DNA-technology
    • C12N15/63Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/67General methods for enhancing the expression
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14Hydrolases (3)
    • C12N9/16Hydrolases (3) acting on ester bonds (3.1)
    • C12N9/22Ribonucleases RNAses, DNAses
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12PFERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P21/00Preparation of peptides or proteins
    • C12P21/02Preparation of peptides or proteins having a known sequence of two or more amino acids, e.g. glutathione
    • AHUMAN NECESSITIES
    • A61MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61KPREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K38/00Medicinal preparations containing peptides
    • AHUMAN NECESSITIES
    • A61MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61KPREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K48/00Medicinal preparations containing genetic material which is inserted into cells of the living body to treat genetic diseases; Gene therapy

Landscapes

  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Organic Chemistry (AREA)
  • Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • Molecular Biology (AREA)
  • Microbiology (AREA)
  • Biomedical Technology (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Medicinal Chemistry (AREA)
  • Physics & Mathematics (AREA)
  • Biophysics (AREA)
  • Plant Pathology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • General Chemical & Material Sciences (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Peptides Or Proteins (AREA)
FR9512162A 1995-10-17 1995-10-17 Polypeptide precurseur d'adn gyrase contenant une inteine Expired - Fee Related FR2739859B1 (fr)

Priority Applications (1)

Application Number Priority Date Filing Date Title
FR9512162A FR2739859B1 (fr) 1995-10-17 1995-10-17 Polypeptide precurseur d'adn gyrase contenant une inteine

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
FR9512162A FR2739859B1 (fr) 1995-10-17 1995-10-17 Polypeptide precurseur d'adn gyrase contenant une inteine

Publications (2)

Publication Number Publication Date
FR2739859A1 FR2739859A1 (fr) 1997-04-18
FR2739859B1 true FR2739859B1 (fr) 1998-01-02

Family

ID=9483602

Family Applications (1)

Application Number Title Priority Date Filing Date
FR9512162A Expired - Fee Related FR2739859B1 (fr) 1995-10-17 1995-10-17 Polypeptide precurseur d'adn gyrase contenant une inteine

Country Status (1)

Country Link
FR (1) FR2739859B1 (fr)

Families Citing this family (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US6521425B2 (en) * 1997-03-05 2003-02-18 New England Biolabs, Inc. Screening and use of reagents which block or activate intein splicing utilizing natural or homologous exteins
DE19916227A1 (de) * 1999-04-10 2000-11-02 Merlin Mikrobiolog Diag Gmbh Verfahren zur genotypischen Klassifizierung
NZ511799A (en) * 2001-05-18 2004-01-30 Univ Otago A eukaryotic intein (Cne PRP8) from Cryptococcus neoformans and potential use as a molecular target for treatment of severe microbial infection

Family Cites Families (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5070192A (en) * 1988-03-23 1991-12-03 The Johns Hopkins University Cloned human topoisomerase i: cdna expression, and use for autoantibody detection
US5427928A (en) * 1993-03-24 1995-06-27 Slesarev; Alexei I. Thermostable DNA Topoisomerase V

Also Published As

Publication number Publication date
FR2739859A1 (fr) 1997-04-18

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Legal Events

Date Code Title Description
ST Notification of lapse