FR2726574B1 - Procede de dissociation des cellules eucaryotes en culture - Google Patents
Procede de dissociation des cellules eucaryotes en cultureInfo
- Publication number
- FR2726574B1 FR2726574B1 FR9413249A FR9413249A FR2726574B1 FR 2726574 B1 FR2726574 B1 FR 2726574B1 FR 9413249 A FR9413249 A FR 9413249A FR 9413249 A FR9413249 A FR 9413249A FR 2726574 B1 FR2726574 B1 FR 2726574B1
- Authority
- FR
- France
- Prior art keywords
- dissociation
- culture
- eukaryotic cells
- eukaryotic
- cells
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Expired - Fee Related
Links
- 238000010494 dissociation reaction Methods 0.000 title 1
- 230000005593 dissociations Effects 0.000 title 1
- 238000000034 method Methods 0.000 title 1
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
- C12N5/0068—General culture methods using substrates
- C12N5/0075—General culture methods using substrates using microcarriers
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N1/00—Preservation of bodies of humans or animals, or parts thereof
- A01N1/02—Preservation of living parts
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N1/00—Preservation of bodies of humans or animals, or parts thereof
- A01N1/02—Preservation of living parts
- A01N1/0205—Chemical aspects
- A01N1/021—Preservation or perfusion media, liquids, solids or gases used in the preservation of cells, tissue, organs or bodily fluids
- A01N1/0221—Freeze-process protecting agents, i.e. substances protecting cells from effects of the physical process, e.g. cryoprotectants, osmolarity regulators like oncotic agents
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
- C12N5/0068—General culture methods using substrates
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N2501/00—Active agents used in cell culture processes, e.g. differentation
- C12N2501/90—Polysaccharides
- C12N2501/91—Heparin
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N2509/00—Methods for the dissociation of cells, e.g. specific use of enzymes
Landscapes
- Life Sciences & Earth Sciences (AREA)
- Engineering & Computer Science (AREA)
- Health & Medical Sciences (AREA)
- Wood Science & Technology (AREA)
- Zoology (AREA)
- Genetics & Genomics (AREA)
- Chemical & Material Sciences (AREA)
- Organic Chemistry (AREA)
- Biomedical Technology (AREA)
- Biotechnology (AREA)
- Bioinformatics & Cheminformatics (AREA)
- General Health & Medical Sciences (AREA)
- Biochemistry (AREA)
- Microbiology (AREA)
- Cell Biology (AREA)
- General Engineering & Computer Science (AREA)
- Dentistry (AREA)
- Environmental Sciences (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
Priority Applications (5)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
FR9413249A FR2726574B1 (fr) | 1994-11-04 | 1994-11-04 | Procede de dissociation des cellules eucaryotes en culture |
PCT/FR1995/001435 WO1996014395A1 (fr) | 1994-11-04 | 1995-10-31 | Procede de dissociation des cellules eucaryotes en culture |
JP8515089A JPH10510144A (ja) | 1994-11-04 | 1995-10-31 | 培養真核細胞の解離方法 |
EP95939309A EP0789756A1 (fr) | 1994-11-04 | 1995-10-31 | Procede de dissociation des cellules eucaryotes en culture |
US08/836,596 US5906939A (en) | 1994-11-04 | 1995-10-31 | Cultured fibroblast or epithelial cell dissociation method using sulfated polysaccharide and chelator |
Applications Claiming Priority (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
FR9413249A FR2726574B1 (fr) | 1994-11-04 | 1994-11-04 | Procede de dissociation des cellules eucaryotes en culture |
Publications (2)
Publication Number | Publication Date |
---|---|
FR2726574A1 FR2726574A1 (fr) | 1996-05-10 |
FR2726574B1 true FR2726574B1 (fr) | 1997-01-17 |
Family
ID=9468535
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
FR9413249A Expired - Fee Related FR2726574B1 (fr) | 1994-11-04 | 1994-11-04 | Procede de dissociation des cellules eucaryotes en culture |
Country Status (5)
Country | Link |
---|---|
US (1) | US5906939A (fr) |
EP (1) | EP0789756A1 (fr) |
JP (1) | JPH10510144A (fr) |
FR (1) | FR2726574B1 (fr) |
WO (1) | WO1996014395A1 (fr) |
Families Citing this family (4)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
JP2000517188A (ja) | 1996-08-30 | 2000-12-26 | ライフ テクノロジーズ,インコーポレイテッド | 無血清哺乳動物細胞培養培地およびその使用 |
EP1484409B1 (fr) * | 1996-12-16 | 2009-04-08 | Eisai R&D Management Co., Ltd. | Construction d'AND comprenant un gène de résistance à un médicament et un gène étranger |
JP4698112B2 (ja) * | 2000-03-03 | 2011-06-08 | 一般財団法人化学及血清療法研究所 | 無血清培養かつ浮遊培養可能な細胞及び当該細胞を用いたワクチン用ウイルスの製造法 |
WO2006109367A1 (fr) * | 2005-04-05 | 2006-10-19 | Obihiro University Of Agriculture And Veterinary Medicine | Méthode de libération cellulaire, solution de libération cellulaire, méthode de culture cellulaire, milieu de culture cellulaire, solution cellulaire, préparation de solution cellulaire, méthode de colonisation cellulaire et solution de colonisation cellulaire |
Family Cites Families (4)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
EP0142344A3 (fr) * | 1983-11-10 | 1986-10-08 | The Wistar Institute Of Anatomy And Biology | Milieu pour le clonage et la culture en série à long terme de cellules endothéliales humaines |
US5024947A (en) * | 1987-07-24 | 1991-06-18 | Cetus Corporation | Serum free media for the growth on insect cells and expression of products thereby |
DE3938907C2 (de) * | 1989-11-24 | 1999-11-04 | Dade Behring Marburg Gmbh | Mittel zum Lagern und Suspendieren von Zellen, insbesondere Erythrozyten |
US5348877A (en) * | 1993-03-12 | 1994-09-20 | Boyce Thompson Institute For Plant Research, Inc. | Method of adapting anchorage-dependent cell lines to suspension conditions |
-
1994
- 1994-11-04 FR FR9413249A patent/FR2726574B1/fr not_active Expired - Fee Related
-
1995
- 1995-10-31 JP JP8515089A patent/JPH10510144A/ja active Pending
- 1995-10-31 US US08/836,596 patent/US5906939A/en not_active Expired - Lifetime
- 1995-10-31 EP EP95939309A patent/EP0789756A1/fr not_active Withdrawn
- 1995-10-31 WO PCT/FR1995/001435 patent/WO1996014395A1/fr not_active Application Discontinuation
Also Published As
Publication number | Publication date |
---|---|
EP0789756A1 (fr) | 1997-08-20 |
WO1996014395A1 (fr) | 1996-05-17 |
FR2726574A1 (fr) | 1996-05-10 |
US5906939A (en) | 1999-05-25 |
JPH10510144A (ja) | 1998-10-06 |
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Legal Events
Date | Code | Title | Description |
---|---|---|---|
ST | Notification of lapse |