FR2422719A1 - PROCESS ALLOWING THE MICROBIAL EXPRESSION OF POLYPEPTIDES AND APPROPRIATE MEANS - Google Patents
PROCESS ALLOWING THE MICROBIAL EXPRESSION OF POLYPEPTIDES AND APPROPRIATE MEANSInfo
- Publication number
- FR2422719A1 FR2422719A1 FR7916726A FR7916726A FR2422719A1 FR 2422719 A1 FR2422719 A1 FR 2422719A1 FR 7916726 A FR7916726 A FR 7916726A FR 7916726 A FR7916726 A FR 7916726A FR 2422719 A1 FR2422719 A1 FR 2422719A1
- Authority
- FR
- France
- Prior art keywords
- expression
- polypeptides
- appropriate means
- process allowing
- microbial expression
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Granted
Links
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
- C12N15/62—DNA sequences coding for fusion proteins
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07H—SUGARS; DERIVATIVES THEREOF; NUCLEOSIDES; NUCLEOTIDES; NUCLEIC ACIDS
- C07H21/00—Compounds containing two or more mononucleotide units having separate phosphate or polyphosphate groups linked by saccharide radicals of nucleoside groups, e.g. nucleic acids
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07H—SUGARS; DERIVATIVES THEREOF; NUCLEOSIDES; NUCLEOTIDES; NUCLEIC ACIDS
- C07H21/00—Compounds containing two or more mononucleotide units having separate phosphate or polyphosphate groups linked by saccharide radicals of nucleoside groups, e.g. nucleic acids
- C07H21/04—Compounds containing two or more mononucleotide units having separate phosphate or polyphosphate groups linked by saccharide radicals of nucleoside groups, e.g. nucleic acids with deoxyribosyl as saccharide radical
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
- C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
- C07K14/575—Hormones
- C07K14/62—Insulins
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
- C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
- C07K14/575—Hormones
- C07K14/655—Somatostatins
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K2319/00—Fusion polypeptide
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K2319/00—Fusion polypeptide
- C07K2319/61—Fusion polypeptide containing an enzyme fusion for detection (lacZ, luciferase)
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K2319/00—Fusion polypeptide
- C07K2319/70—Fusion polypeptide containing domain for protein-protein interaction
- C07K2319/74—Fusion polypeptide containing domain for protein-protein interaction containing a fusion for binding to a cell surface receptor
- C07K2319/75—Fusion polypeptide containing domain for protein-protein interaction containing a fusion for binding to a cell surface receptor containing a fusion for activation of a cell surface receptor, e.g. thrombopoeitin, NPY and other peptide hormones
Landscapes
- Health & Medical Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Organic Chemistry (AREA)
- Genetics & Genomics (AREA)
- Molecular Biology (AREA)
- Biochemistry (AREA)
- Engineering & Computer Science (AREA)
- General Health & Medical Sciences (AREA)
- Zoology (AREA)
- Biotechnology (AREA)
- Biomedical Technology (AREA)
- Biophysics (AREA)
- Medicinal Chemistry (AREA)
- General Engineering & Computer Science (AREA)
- Gastroenterology & Hepatology (AREA)
- Toxicology (AREA)
- Endocrinology (AREA)
- Wood Science & Technology (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Plant Pathology (AREA)
- Diabetes (AREA)
- Microbiology (AREA)
- Physics & Mathematics (AREA)
- Preparation Of Compounds By Using Micro-Organisms (AREA)
- Medicines Containing Antibodies Or Antigens For Use As Internal Diagnostic Agents (AREA)
Abstract
L'invention décrit des vecteurs de clonage microbiens recombinants comprenant de l'ADN hétérologue codant l'expression d'une polypeptide (la somatostatine sur le schéma général de la figure 1), comprenant des plasmides permettant la transformation des hôtes bactériens; des vecteurs de clonage codant l'expression d'un haptène polypeptidique et d'une protéine additionnelle conférant un caractère immunogène au produit qui peut ou non être coupé en ses deux éléments; et des procédés de préparation de gènes de structure synthétiques. Application, entre autres, à la synthèse d'hormones et de vaccins.The invention describes recombinant microbial cloning vectors comprising heterologous DNA encoding the expression of a polypeptide (somatostatin in the general scheme of FIG. 1), comprising plasmids allowing the transformation of bacterial hosts; cloning vectors encoding the expression of a polypeptide hapten and of an additional protein conferring immunogenicity on the product which may or may not be cut into its two elements; and methods of preparing synthetic structural genes. Application, among others, to the synthesis of hormones and vaccines.
Applications Claiming Priority (3)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
US84969277A | 1977-11-08 | 1977-11-08 | |
US84959177A | 1977-11-08 | 1977-11-08 | |
US84969177A | 1977-11-08 | 1977-11-08 |
Publications (2)
Publication Number | Publication Date |
---|---|
FR2422719A1 true FR2422719A1 (en) | 1979-11-09 |
FR2422719B1 FR2422719B1 (en) | 1984-12-28 |
Family
ID=27420338
Family Applications (3)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
FR7831350A Expired FR2422717B1 (en) | 1977-11-08 | 1978-11-06 | PROCESS FOR THE MICROBIAL EXPRESSION OF POLYPEPTIDES AND APPROPRIATE MEANS |
FR7916726A Granted FR2422719A1 (en) | 1977-11-08 | 1979-06-28 | PROCESS ALLOWING THE MICROBIAL EXPRESSION OF POLYPEPTIDES AND APPROPRIATE MEANS |
FR7916725A Granted FR2422718A1 (en) | 1977-11-08 | 1979-06-28 | PROCESS ALLOWING THE MOCROBIAL EXPRESSION OF POLYPEPTIDES AND APPROPRIATE MEANS |
Family Applications Before (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
FR7831350A Expired FR2422717B1 (en) | 1977-11-08 | 1978-11-06 | PROCESS FOR THE MICROBIAL EXPRESSION OF POLYPEPTIDES AND APPROPRIATE MEANS |
Family Applications After (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
FR7916725A Granted FR2422718A1 (en) | 1977-11-08 | 1979-06-28 | PROCESS ALLOWING THE MOCROBIAL EXPRESSION OF POLYPEPTIDES AND APPROPRIATE MEANS |
Country Status (7)
Country | Link |
---|---|
AU (2) | AU527165B2 (en) |
BR (1) | BR7807290A (en) |
FR (3) | FR2422717B1 (en) |
LU (1) | LU80489A1 (en) |
OA (1) | OA06002A (en) |
RO (1) | RO80052B (en) |
ZM (1) | ZM9578A1 (en) |
Families Citing this family (6)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
DK159976C (en) * | 1979-06-01 | 1991-05-27 | Searle & Co | PLASMID VECTORS, METHOD OF PRODUCING THEREOF, BACTERY CELLS TRANSFORMED WITH PLASMID VECTORS, AND EXPRESSION OF PROTEIN IN TRANSFORMED BACTERY CELLS |
US5254463A (en) * | 1981-09-18 | 1993-10-19 | Genentech, Inc. | Method for expression of bovine growth hormone |
US4645828A (en) * | 1984-03-23 | 1987-02-24 | Oncogen | Platelet related growth regulator |
AU580064B2 (en) * | 1984-06-04 | 1988-12-22 | Lubrizol Genetics Inc. | Nitrogen fixation regulator genes |
US5489529A (en) * | 1984-07-19 | 1996-02-06 | De Boer; Herman A. | DNA for expression of bovine growth hormone |
DK175535B1 (en) * | 1987-03-04 | 2004-11-29 | Daiichi Suntory Pharma Co Ltd | Process for preparing a physiologically active cysteine-containing peptide |
Citations (1)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
FR2281426A1 (en) * | 1974-08-08 | 1976-03-05 | Ici Ltd | PROCESS FOR MODIFICATION OF DESOXY-RIBONUCLEIC ACID AND PRODUCTS OBTAINED |
Family Cites Families (8)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
NZ187300A (en) * | 1977-05-27 | 1982-08-17 | Univ California | Dna transfer vector and micro-organism modified to contain a nucleotide sequence equivalent to the gene of a higher organism |
ZA782933B (en) * | 1977-09-23 | 1979-05-30 | Univ California | Purification of nucleotide sequences suitable for expression in bacteria |
BR7807288A (en) * | 1977-11-08 | 1979-06-12 | Genentech Inc | POLYNUCLEOTIDE SYNTHESIS PROCESS |
FR2422685A1 (en) * | 1978-04-14 | 1979-11-09 | Pasteur Institut | VECTORS, DERIVED FROM LAMBDA BACTERIOPHAGUS, ALLOWING THE INSERTION AND EXPRESSION OF A GENE, PROCARYOTE OR EUKARYOTE, UNDER THE CONTROL OF THE PROMOTER AND THE OPERATOR OF THE LACTOSE OPERON OF ESCHERICHIA COLI BACTERIA |
FR2428075A1 (en) * | 1978-06-08 | 1980-01-04 | Pasteur Institut | PROCESS FOR THE PRODUCTION OF PROTEINS BY EXPRESSION OF THE CORRESPONDING GENES IN BACTERIA AND VECTORS LIKELY TO BE USED IN SUCH METHODS |
US4411994A (en) * | 1978-06-08 | 1983-10-25 | The President And Fellows Of Harvard College | Protein synthesis |
FI792481A (en) * | 1978-08-11 | 1980-02-12 | Univ California | SYNTHESIS OF ENCAROYTIC PROTEIN GENOM ANVAENDNING AV MICRO-ORGANISM |
GB2033905B (en) * | 1978-08-21 | 1982-10-13 | Upjohn Co | Bacterial preparation of proteins |
-
1978
- 1978-11-06 RO RO95604A patent/RO80052B/en unknown
- 1978-11-06 BR BR7807290A patent/BR7807290A/en unknown
- 1978-11-06 AU AU41361/78A patent/AU527165B2/en not_active Expired
- 1978-11-06 FR FR7831350A patent/FR2422717B1/en not_active Expired
- 1978-11-08 LU LU80489A patent/LU80489A1/en unknown
- 1978-11-08 OA OA56652A patent/OA06002A/en unknown
- 1978-11-08 ZM ZM95/78A patent/ZM9578A1/en unknown
-
1979
- 1979-06-28 FR FR7916726A patent/FR2422719A1/en active Granted
- 1979-06-28 FR FR7916725A patent/FR2422718A1/en active Granted
-
1981
- 1981-08-24 AU AU74484/81A patent/AU546092B2/en not_active Expired
Patent Citations (1)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
FR2281426A1 (en) * | 1974-08-08 | 1976-03-05 | Ici Ltd | PROCESS FOR MODIFICATION OF DESOXY-RIBONUCLEIC ACID AND PRODUCTS OBTAINED |
Non-Patent Citations (5)
Title |
---|
CHEMICAL AND ENGINEERING NEWS, volume 50, no. 45, 7 novembre 1977 "Human gene in E.coli: it works", page 4 * |
NATURE, volume 263, 28 octobre 1976 K.J. MARIANS et al. "Cloned synthetic lac operator DNA is biologically active", pages 744-748 * |
PROC. NATL. ACAD. SCI, volume 73, no. 9, septembre 1976 (US) R. HIGUCHI et al. "A general method for cloning eukaryotic structural gene sequences", pages 3146-3150 * |
SCIENCE, volume 196, no. 4296, 11 juin 1977, A. ULLRICH et al. "Rat insulin genes: Construction of plasmids containing the coding sequences", pages 1 313-1319 * |
SCIENCE, volume 198, no. 4321, 9 décembre 1977, K. ITAKURA et al. "Expression in Escherichia coli of a chemically synthesized gene for the hormone somatostatin" pages 1056-1063 * |
Also Published As
Publication number | Publication date |
---|---|
LU80489A1 (en) | 1979-03-22 |
FR2422717B1 (en) | 1985-12-06 |
RO80052B (en) | 1985-03-30 |
AU7448481A (en) | 1982-01-14 |
ZM9578A1 (en) | 1980-03-21 |
AU527165B2 (en) | 1983-02-17 |
FR2422718B1 (en) | 1984-05-18 |
OA06002A (en) | 1981-06-30 |
AU546092B2 (en) | 1985-08-15 |
FR2422717A1 (en) | 1979-11-09 |
FR2422718A1 (en) | 1979-11-09 |
AU4136178A (en) | 1979-05-17 |
BR7807290A (en) | 1979-06-12 |
RO80052A (en) | 1985-03-15 |
FR2422719B1 (en) | 1984-12-28 |
Similar Documents
Publication | Publication Date | Title |
---|---|---|
NL300197I1 (en) | ||
EP0518650A3 (en) | Sequence specific dna binding by p53 | |
EP0217919A4 (en) | Type-specific papillomavirus dna sequences and peptides. | |
NZ502391A (en) | Mammalian cytokine: interleukin-B30 and related reagents | |
NZ218332A (en) | Il-4(interleukin-4), recombinant production, dna, vectors and pharmaceutical composition | |
ATE133681T1 (en) | MYCOBACTERIAL RECOMBINANTS AND PEPTIDES | |
ATE54167T1 (en) | PROMOTERS FOR THE EXPRESSION OF FOREIGN GENES IN YEAST, PLASMIDS CONTAINING THESE PROMOTERS AND THEIR USE TO PRODUCTION POLYPEPTIDES. | |
EP1005540A4 (en) | Ikk-beta proteins, nucleic acids and methods | |
ES8500325A1 (en) | Preparation of functional human urokinase polypeptides. | |
ES8802249A1 (en) | Molecular cloning and characterization of a further gene sequence coding for human relaxin. | |
DE68922917D1 (en) | KEX2 endoprotease and process for its preparation. | |
CA2166691A1 (en) | Bacillus thuringiensis transposon tn5401 and its use in a site-specific recombination system for bacillus thuringiensis strain development | |
ES8801671A1 (en) | Molecular cloning and characterization of a gene sequence coding for human relaxin. | |
AU7565991A (en) | Variant cd44 surface proteins, dna sequences coding them, antibodies against these proteins and their use in diagnosis and therapy | |
AU8223387A (en) | Horse gamma-interferon | |
FR2422718A1 (en) | PROCESS ALLOWING THE MOCROBIAL EXPRESSION OF POLYPEPTIDES AND APPROPRIATE MEANS | |
IE801912L (en) | Gaming machine | |
DE3585578D1 (en) | HEPATITIS SURFACE ANTI-PARTICLE VACCINE. | |
KE3747A (en) | Recombinant dna,hosts transformed with it and polypeptides produced by the hosts;processes for their preparation;detection processes using the polypeptides;compositions and means containing the polypeptides;dna sequences | |
EP0261534A3 (en) | Improved gene expression in yeast | |
DE3380495D1 (en) | Molecular cloning and characterization of the gene sequence coding for porcine relaxin | |
ATE103979T1 (en) | HUMAN LYMPHOTOXIN. | |
DE3787565D1 (en) | PLASMIDES THAT GIVE PHAGE SENSITIVITY TO BACTERIA. | |
Peberdy | Genetic engineering in relation to enzymes. | |
Song et al. | Chromosomal Mapping of the Gene Encoding Deoxycytidine-Cytidine Deaminase in Bacillus subtilis |
Legal Events
Date | Code | Title | Description |
---|---|---|---|
ST | Notification of lapse |