FR2422685A1 - Vecteurs, derives du bacteriophage lambda, permettant l'insertion et l'expression d'un gene, procaryote ou eucaryote, sous le controle du promoteur et de l'operateur de l'operon lactose de la bacterie escherichia coli - Google Patents

Vecteurs, derives du bacteriophage lambda, permettant l'insertion et l'expression d'un gene, procaryote ou eucaryote, sous le controle du promoteur et de l'operateur de l'operon lactose de la bacterie escherichia coli

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Publication number
FR2422685A1
FR2422685A1 FR7811109A FR7811109A FR2422685A1 FR 2422685 A1 FR2422685 A1 FR 2422685A1 FR 7811109 A FR7811109 A FR 7811109A FR 7811109 A FR7811109 A FR 7811109A FR 2422685 A1 FR2422685 A1 FR 2422685A1
Authority
FR
France
Prior art keywords
gene
promoter
expression
escherichia coli
bacteriophagus
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Granted
Application number
FR7811109A
Other languages
English (en)
Other versions
FR2422685B1 (fr
Inventor
Pierre Tiollais
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Institut Pasteur de Lille
Original Assignee
Institut Pasteur de Lille
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Institut Pasteur de Lille filed Critical Institut Pasteur de Lille
Priority to FR7811109A priority Critical patent/FR2422685A1/fr
Priority to GB7913033A priority patent/GB2018778B/en
Priority to NL7902910A priority patent/NL7902910A/xx
Priority to DE19792915081 priority patent/DE2915081A1/de
Priority to JP4443179A priority patent/JPS54141790A/ja
Priority to BE0/194621A priority patent/BE875596A/fr
Priority to IT21839/79A priority patent/IT1119726B/it
Publication of FR2422685A1 publication Critical patent/FR2422685A1/fr
Application granted granted Critical
Publication of FR2422685B1 publication Critical patent/FR2422685B1/fr
Granted legal-status Critical Current

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Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor

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  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Genetics & Genomics (AREA)
  • Wood Science & Technology (AREA)
  • Biomedical Technology (AREA)
  • Organic Chemistry (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • Chemical & Material Sciences (AREA)
  • Zoology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biophysics (AREA)
  • Microbiology (AREA)
  • Plant Pathology (AREA)
  • Molecular Biology (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Physics & Mathematics (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Saccharide Compounds (AREA)

Abstract

ADN modifié de bactériophage lambda . Il comprend des mutations Qam73 et Sam7 ayant pour effet de prévenir ou retarder l'expression des gènes tardifs, un fragment d'ADN comportant une partie au moins du gène Z de Escherichia coli et un promoteur d'un opéron bactérien, notamment de l'opéron lactose dans une partie non essentielle du génome du phase, dans le gène Z, un site de coupure par une endonucléase telle que Ecori, à l'exclusion de tout autre site du même type dans l'ADN de phage. Application à l'induction par une bactérie de synthèse protéique dirigée.
FR7811109A 1978-04-14 1978-04-14 Vecteurs, derives du bacteriophage lambda, permettant l'insertion et l'expression d'un gene, procaryote ou eucaryote, sous le controle du promoteur et de l'operateur de l'operon lactose de la bacterie escherichia coli Granted FR2422685A1 (fr)

Priority Applications (7)

Application Number Priority Date Filing Date Title
FR7811109A FR2422685A1 (fr) 1978-04-14 1978-04-14 Vecteurs, derives du bacteriophage lambda, permettant l'insertion et l'expression d'un gene, procaryote ou eucaryote, sous le controle du promoteur et de l'operateur de l'operon lactose de la bacterie escherichia coli
GB7913033A GB2018778B (en) 1978-04-14 1979-04-12 Vectors derived from lambda bacteriophage
NL7902910A NL7902910A (nl) 1978-04-14 1979-04-12 Gemodificeerd desoxyribonucleinezuur uit lambda-bacte- riofagen.
DE19792915081 DE2915081A1 (de) 1978-04-14 1979-04-12 Modifizierte dna des phagen lambda und ihre verwendung zur herstellung von proteinen
JP4443179A JPS54141790A (en) 1978-04-14 1979-04-13 Modified dna and method of manufacturing hybrid protein using it
BE0/194621A BE875596A (fr) 1978-04-14 1979-04-13 Vecteurs, derives du bacteriophage lambda, permettant l'insertion et l'expression d'un gene, procaryote ou eucaryote
IT21839/79A IT1119726B (it) 1978-04-14 1979-04-13 Vettori,derivati dal batteriofago lambada,che permettono l'inserimento e l'espressivita' di un gene,procariota o eucariota,sotto il controllo del promotore e dell'operatore dell'operon lattosio del batterio escherichia coli

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
FR7811109A FR2422685A1 (fr) 1978-04-14 1978-04-14 Vecteurs, derives du bacteriophage lambda, permettant l'insertion et l'expression d'un gene, procaryote ou eucaryote, sous le controle du promoteur et de l'operateur de l'operon lactose de la bacterie escherichia coli

Publications (2)

Publication Number Publication Date
FR2422685A1 true FR2422685A1 (fr) 1979-11-09
FR2422685B1 FR2422685B1 (fr) 1980-10-31

Family

ID=9207171

Family Applications (1)

Application Number Title Priority Date Filing Date
FR7811109A Granted FR2422685A1 (fr) 1978-04-14 1978-04-14 Vecteurs, derives du bacteriophage lambda, permettant l'insertion et l'expression d'un gene, procaryote ou eucaryote, sous le controle du promoteur et de l'operateur de l'operon lactose de la bacterie escherichia coli

Country Status (7)

Country Link
JP (1) JPS54141790A (fr)
BE (1) BE875596A (fr)
DE (1) DE2915081A1 (fr)
FR (1) FR2422685A1 (fr)
GB (1) GB2018778B (fr)
IT (1) IT1119726B (fr)
NL (1) NL7902910A (fr)

Cited By (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
FR2422718A1 (fr) * 1977-11-08 1979-11-09 Genentech Inc Procede permettant l'expression mocrobienne de polypeptides et moyens appropries
FR2480779A2 (fr) * 1979-08-30 1981-10-23 Anvar Vecteur contenant une sequence nucleotidique de l'antigene de surface du virus de l'hepatite b et procede de fabrication d'une molecule immunogene mettant en oeuvre ce vecteur

Families Citing this family (8)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US6268122B1 (en) 1978-12-22 2001-07-31 Biogen, Inc. Recombinant DNA molecules and their method of production
US4874702A (en) * 1980-09-08 1989-10-17 Biogen, Inc. Vectors and methods for making such vectors and for expressive cloned genes
AU8746582A (en) * 1981-09-02 1983-03-10 Biogen N.V. Hepatitis b virus e type antigen
US4460689A (en) * 1982-04-15 1984-07-17 Merck & Co., Inc. DNA Cloning vector TG1, derivatives, and processes of making
US4508826A (en) * 1982-04-15 1985-04-02 Merck & Co., Inc. Bacteriophage DNA cloning vector TG1 and microorganisms containing TG1
JPS58212781A (ja) * 1982-06-02 1983-12-10 Noda Sangyo Kagaku Kenkyusho 新種バクテリオフア−ジ及びその育種法
ATE60803T1 (de) * 1983-06-27 1991-02-15 Genentech Inc Uebertragbare induzierbare kontrollsysteme, diese enthaltende expressionsvektoren, mit diesen transformierte mikroorganismen und ihre verwendung bei der expression von exogenem protein.
CA1278540C (fr) * 1983-07-22 1991-01-02 Eli Lilly And Company Gene modifie conferant une resistance a un antibiotique

Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
DE2644432A1 (de) * 1976-09-27 1978-03-30 Research Corp Genetisch modifizierte mikroorganismen und verfahren zu ihrer herstellung

Patent Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
DE2644432A1 (de) * 1976-09-27 1978-03-30 Research Corp Genetisch modifizierte mikroorganismen und verfahren zu ihrer herstellung

Cited By (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
FR2422718A1 (fr) * 1977-11-08 1979-11-09 Genentech Inc Procede permettant l'expression mocrobienne de polypeptides et moyens appropries
FR2480779A2 (fr) * 1979-08-30 1981-10-23 Anvar Vecteur contenant une sequence nucleotidique de l'antigene de surface du virus de l'hepatite b et procede de fabrication d'une molecule immunogene mettant en oeuvre ce vecteur

Also Published As

Publication number Publication date
GB2018778A (en) 1979-10-24
BE875596A (fr) 1979-10-15
NL7902910A (nl) 1979-10-16
IT7921839A0 (it) 1979-04-13
IT1119726B (it) 1986-03-10
DE2915081A1 (de) 1979-10-25
JPS54141790A (en) 1979-11-05
GB2018778B (en) 1982-08-25
FR2422685B1 (fr) 1980-10-31

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