FR2403559A1 - Haemagglutination reagent for determn. of cholera toxin - comprising specific ganglioside coupled to erythrocytes - Google Patents

Haemagglutination reagent for determn. of cholera toxin - comprising specific ganglioside coupled to erythrocytes

Info

Publication number
FR2403559A1
FR2403559A1 FR7728164A FR7728164A FR2403559A1 FR 2403559 A1 FR2403559 A1 FR 2403559A1 FR 7728164 A FR7728164 A FR 7728164A FR 7728164 A FR7728164 A FR 7728164A FR 2403559 A1 FR2403559 A1 FR 2403559A1
Authority
FR
France
Prior art keywords
cholera toxin
determn
coupled
haemagglutination
erythrocytes
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Granted
Application number
FR7728164A
Other languages
French (fr)
Other versions
FR2403559B1 (en
Inventor
Jean-Louis Tayot
Marie-Claude Mynard
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Institut Merieux SA
Original Assignee
Institut Merieux SA
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Institut Merieux SA filed Critical Institut Merieux SA
Priority to FR7728164A priority Critical patent/FR2403559A1/en
Publication of FR2403559A1 publication Critical patent/FR2403559A1/en
Application granted granted Critical
Publication of FR2403559B1 publication Critical patent/FR2403559B1/fr
Granted legal-status Critical Current

Links

Classifications

    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/543—Immunoassay; Biospecific binding assay; Materials therefor with an insoluble carrier for immobilising immunochemicals
    • G01N33/554—Immunoassay; Biospecific binding assay; Materials therefor with an insoluble carrier for immobilising immunochemicals the carrier being a biological cell or cell fragment, e.g. bacteria, yeast cells
    • G01N33/555—Red blood cell

Landscapes

  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Immunology (AREA)
  • Engineering & Computer Science (AREA)
  • Cell Biology (AREA)
  • Hematology (AREA)
  • Biomedical Technology (AREA)
  • Molecular Biology (AREA)
  • Chemical & Material Sciences (AREA)
  • Urology & Nephrology (AREA)
  • Microbiology (AREA)
  • Physics & Mathematics (AREA)
  • Biotechnology (AREA)
  • Food Science & Technology (AREA)
  • Medicinal Chemistry (AREA)
  • Mycology (AREA)
  • Analytical Chemistry (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • General Physics & Mathematics (AREA)
  • Pathology (AREA)
  • Investigating Or Analysing Biological Materials (AREA)
  • Medicines Containing Material From Animals Or Micro-Organisms (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)

Abstract

Biological reagents and their use for the determn. of cholera toxin (choleragen or choleragenoid), cholera toxin antibodies and ganglioside GM1 (I) or its derivs. are claimed. The reagents comprise erythrocytes treated with a carbonyl cpd. and coupled with (I) or its derivs., and opt. coupled with a sub-agglutinating amt. of cholera toxin. The reagents have a long shelf-life (>=1 year at 4 degrees C), can be freeze-dried, can be prepd. using small amts. of crude (I), and have high sensitivity for cholera toxin determn. (a few ng/ml).
FR7728164A 1977-09-19 1977-09-19 Haemagglutination reagent for determn. of cholera toxin - comprising specific ganglioside coupled to erythrocytes Granted FR2403559A1 (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
FR7728164A FR2403559A1 (en) 1977-09-19 1977-09-19 Haemagglutination reagent for determn. of cholera toxin - comprising specific ganglioside coupled to erythrocytes

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
FR7728164A FR2403559A1 (en) 1977-09-19 1977-09-19 Haemagglutination reagent for determn. of cholera toxin - comprising specific ganglioside coupled to erythrocytes

Publications (2)

Publication Number Publication Date
FR2403559A1 true FR2403559A1 (en) 1979-04-13
FR2403559B1 FR2403559B1 (en) 1982-12-17

Family

ID=9195523

Family Applications (1)

Application Number Title Priority Date Filing Date
FR7728164A Granted FR2403559A1 (en) 1977-09-19 1977-09-19 Haemagglutination reagent for determn. of cholera toxin - comprising specific ganglioside coupled to erythrocytes

Country Status (1)

Country Link
FR (1) FR2403559A1 (en)

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
FR2577048A1 (en) * 1985-02-05 1986-08-08 Pasteur Institut REAGENT FOR HEMAGGLUTINATION ASSAY OF ANTIBODIES AGAINST BACTERIAL TOXINS, PREPARATION METHOD AND APPLICATION THEREOF

Non-Patent Citations (1)

* Cited by examiner, † Cited by third party
Title
CA1970 *

Cited By (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
FR2577048A1 (en) * 1985-02-05 1986-08-08 Pasteur Institut REAGENT FOR HEMAGGLUTINATION ASSAY OF ANTIBODIES AGAINST BACTERIAL TOXINS, PREPARATION METHOD AND APPLICATION THEREOF
EP0193439A1 (en) * 1985-02-05 1986-09-03 Institut Pasteur Reagent for the determination by haemagglutination of antibodies against bacterial toxins, method for its preparation and its use

Also Published As

Publication number Publication date
FR2403559B1 (en) 1982-12-17

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Legal Events

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