FR2359873A1 - Crosslinked n-methylol-acrylamide gels contg. polysaccharide - on polyester film support, for electrophoretic sepn. of lipoprotein(s) - Google Patents

Crosslinked n-methylol-acrylamide gels contg. polysaccharide - on polyester film support, for electrophoretic sepn. of lipoprotein(s)

Info

Publication number
FR2359873A1
FR2359873A1 FR7622803A FR7622803A FR2359873A1 FR 2359873 A1 FR2359873 A1 FR 2359873A1 FR 7622803 A FR7622803 A FR 7622803A FR 7622803 A FR7622803 A FR 7622803A FR 2359873 A1 FR2359873 A1 FR 2359873A1
Authority
FR
France
Prior art keywords
gels
contg
methylol
polyester film
acrylamide
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Granted
Application number
FR7622803A
Other languages
French (fr)
Other versions
FR2359873B1 (en
Inventor
Egisto Boschetti
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Sebia SA
Original Assignee
Sebia SA
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Sebia SA filed Critical Sebia SA
Priority to FR7622803A priority Critical patent/FR2359873A1/en
Publication of FR2359873A1 publication Critical patent/FR2359873A1/en
Application granted granted Critical
Publication of FR2359873B1 publication Critical patent/FR2359873B1/fr
Granted legal-status Critical Current

Links

Classifications

    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N27/00—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means
    • G01N27/26—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means by investigating electrochemical variables; by using electrolysis or electrophoresis
    • G01N27/416—Systems
    • G01N27/447—Systems using electrophoresis
    • G01N27/44704—Details; Accessories
    • G01N27/44747—Composition of gel or of carrier mixture

Abstract

New hydrated or rehydratable gels laminated onto a polyester film comprise a polymer (I) of N-methylol-acrylamide (II) in the structure of which is included a gellable polysaccharide (III) contg. COOH gps. (I) is formed from (II) and a bifunctional allylic or acrylic agent, esp. N,N'-methylene-bis-acrylamide (IV) for insoluble gels; diallyl-tartramide for gels soluble in 2% HIO4, or ethylene diacrylate for gels soluble in alkali soln. (III) is pref. agarose with strong electroendo-osmotic props. contg. only COOH gps. and is used at 0.1-1%. Used for electrophoretic sepn. of plasma and serum lipoproteins in a discontinuous gradient. Any chylomicrons are completely retained near the sample reservoir; pre-beta-lipoproteins are stopped at the gradient boundary; while the beta-, alpha- and pre-alpha components are sepd. in the more conc. gel. Densitometric measurements are easier on plates than on cylinders, and the plates can be dried after use to give supple films which are easily stored.
FR7622803A 1976-07-27 1976-07-27 Crosslinked n-methylol-acrylamide gels contg. polysaccharide - on polyester film support, for electrophoretic sepn. of lipoprotein(s) Granted FR2359873A1 (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
FR7622803A FR2359873A1 (en) 1976-07-27 1976-07-27 Crosslinked n-methylol-acrylamide gels contg. polysaccharide - on polyester film support, for electrophoretic sepn. of lipoprotein(s)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
FR7622803A FR2359873A1 (en) 1976-07-27 1976-07-27 Crosslinked n-methylol-acrylamide gels contg. polysaccharide - on polyester film support, for electrophoretic sepn. of lipoprotein(s)

Publications (2)

Publication Number Publication Date
FR2359873A1 true FR2359873A1 (en) 1978-02-24
FR2359873B1 FR2359873B1 (en) 1978-12-15

Family

ID=9176172

Family Applications (1)

Application Number Title Priority Date Filing Date
FR7622803A Granted FR2359873A1 (en) 1976-07-27 1976-07-27 Crosslinked n-methylol-acrylamide gels contg. polysaccharide - on polyester film support, for electrophoretic sepn. of lipoprotein(s)

Country Status (1)

Country Link
FR (1) FR2359873A1 (en)

Cited By (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5319046A (en) * 1987-06-13 1994-06-07 Elchrom, Ltd. Polymers, and their use as gels for electrophoresis
WO1995007458A1 (en) * 1993-09-09 1995-03-16 Glyko, Inc. Utilization of additives and defined storage systems to increase the stability and performance of electrophoresis media

Non-Patent Citations (1)

* Cited by examiner, † Cited by third party
Title
NEANT *

Cited By (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5319046A (en) * 1987-06-13 1994-06-07 Elchrom, Ltd. Polymers, and their use as gels for electrophoresis
WO1995007458A1 (en) * 1993-09-09 1995-03-16 Glyko, Inc. Utilization of additives and defined storage systems to increase the stability and performance of electrophoresis media

Also Published As

Publication number Publication date
FR2359873B1 (en) 1978-12-15

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