FR2100517A1 - Purification of lysozyme - by passage through a column of polyacrylam /agarose gel - Google Patents

Purification of lysozyme - by passage through a column of polyacrylam /agarose gel

Info

Publication number
FR2100517A1
FR2100517A1 FR7114089A FR7114089A FR2100517A1 FR 2100517 A1 FR2100517 A1 FR 2100517A1 FR 7114089 A FR7114089 A FR 7114089A FR 7114089 A FR7114089 A FR 7114089A FR 2100517 A1 FR2100517 A1 FR 2100517A1
Authority
FR
France
Prior art keywords
lysozyme
column
purification
gel
agarose gel
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Granted
Application number
FR7114089A
Other languages
French (fr)
Other versions
FR2100517B1 (en
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
SOGERAS
Original Assignee
SOGERAS
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by SOGERAS filed Critical SOGERAS
Priority to FR7114089A priority Critical patent/FR2100517A1/en
Publication of FR2100517A1 publication Critical patent/FR2100517A1/en
Application granted granted Critical
Publication of FR2100517B1 publication Critical patent/FR2100517B1/fr
Granted legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/24—Hydrolases (3) acting on glycosyl compounds (3.2)
    • C12N9/2402—Hydrolases (3) acting on glycosyl compounds (3.2) hydrolysing O- and S- glycosyl compounds (3.2.1)
    • C12N9/2462—Lysozyme (3.2.1.17)

Landscapes

  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Organic Chemistry (AREA)
  • Zoology (AREA)
  • Engineering & Computer Science (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Wood Science & Technology (AREA)
  • Microbiology (AREA)
  • Biotechnology (AREA)
  • Biomedical Technology (AREA)
  • Molecular Biology (AREA)
  • Biochemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Medicinal Chemistry (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)
  • Peptides Or Proteins (AREA)

Abstract

Purification is by passing a soln. of lysozyme contaminated with protein impurities through a column packed with a polyacrylamide/agarose gel in pearl form, and collecting the eluate only after a vol. of soln. equal to the vol. of the pearls has passed through the column. Protein rapidly passes through the gel which acts as a molecular sieve. Lysozyme is retained for some time and then issues freed from protein. The prefd. gel pearls are of 100-160 mu m in dia. and contain 6% acrylamide and 4% agarose. Lysozyme is sepd. from egg albumin readily using this method.
FR7114089A 1971-04-21 1971-04-21 Purification of lysozyme - by passage through a column of polyacrylam /agarose gel Granted FR2100517A1 (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
FR7114089A FR2100517A1 (en) 1971-04-21 1971-04-21 Purification of lysozyme - by passage through a column of polyacrylam /agarose gel

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
FR7114089A FR2100517A1 (en) 1971-04-21 1971-04-21 Purification of lysozyme - by passage through a column of polyacrylam /agarose gel

Publications (2)

Publication Number Publication Date
FR2100517A1 true FR2100517A1 (en) 1972-03-24
FR2100517B1 FR2100517B1 (en) 1973-06-08

Family

ID=9075646

Family Applications (1)

Application Number Title Priority Date Filing Date
FR7114089A Granted FR2100517A1 (en) 1971-04-21 1971-04-21 Purification of lysozyme - by passage through a column of polyacrylam /agarose gel

Country Status (1)

Country Link
FR (1) FR2100517A1 (en)

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP0180164A2 (en) * 1984-10-26 1986-05-07 Reanal Finomvegyszergyar Method for the preparation of lysozyme enzyme

Cited By (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP0180164A2 (en) * 1984-10-26 1986-05-07 Reanal Finomvegyszergyar Method for the preparation of lysozyme enzyme
EP0180164A3 (en) * 1984-10-26 1987-08-05 Reanal Finomvegyszergyar Method for the preparation of lysozyme enzyme

Also Published As

Publication number Publication date
FR2100517B1 (en) 1973-06-08

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