FR2100517A1 - Purification of lysozyme - by passage through a column of polyacrylam /agarose gel - Google Patents
Purification of lysozyme - by passage through a column of polyacrylam /agarose gelInfo
- Publication number
- FR2100517A1 FR2100517A1 FR7114089A FR7114089A FR2100517A1 FR 2100517 A1 FR2100517 A1 FR 2100517A1 FR 7114089 A FR7114089 A FR 7114089A FR 7114089 A FR7114089 A FR 7114089A FR 2100517 A1 FR2100517 A1 FR 2100517A1
- Authority
- FR
- France
- Prior art keywords
- lysozyme
- column
- purification
- gel
- agarose gel
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Granted
Links
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
- C12N9/14—Hydrolases (3)
- C12N9/24—Hydrolases (3) acting on glycosyl compounds (3.2)
- C12N9/2402—Hydrolases (3) acting on glycosyl compounds (3.2) hydrolysing O- and S- glycosyl compounds (3.2.1)
- C12N9/2462—Lysozyme (3.2.1.17)
Landscapes
- Life Sciences & Earth Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Health & Medical Sciences (AREA)
- Genetics & Genomics (AREA)
- Organic Chemistry (AREA)
- Zoology (AREA)
- Engineering & Computer Science (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Wood Science & Technology (AREA)
- Microbiology (AREA)
- Biotechnology (AREA)
- Biomedical Technology (AREA)
- Molecular Biology (AREA)
- Biochemistry (AREA)
- General Engineering & Computer Science (AREA)
- General Health & Medical Sciences (AREA)
- Medicinal Chemistry (AREA)
- Enzymes And Modification Thereof (AREA)
- Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)
- Peptides Or Proteins (AREA)
Abstract
Purification is by passing a soln. of lysozyme contaminated with protein impurities through a column packed with a polyacrylamide/agarose gel in pearl form, and collecting the eluate only after a vol. of soln. equal to the vol. of the pearls has passed through the column. Protein rapidly passes through the gel which acts as a molecular sieve. Lysozyme is retained for some time and then issues freed from protein. The prefd. gel pearls are of 100-160 mu m in dia. and contain 6% acrylamide and 4% agarose. Lysozyme is sepd. from egg albumin readily using this method.
Priority Applications (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
FR7114089A FR2100517A1 (en) | 1971-04-21 | 1971-04-21 | Purification of lysozyme - by passage through a column of polyacrylam /agarose gel |
Applications Claiming Priority (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
FR7114089A FR2100517A1 (en) | 1971-04-21 | 1971-04-21 | Purification of lysozyme - by passage through a column of polyacrylam /agarose gel |
Publications (2)
Publication Number | Publication Date |
---|---|
FR2100517A1 true FR2100517A1 (en) | 1972-03-24 |
FR2100517B1 FR2100517B1 (en) | 1973-06-08 |
Family
ID=9075646
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
FR7114089A Granted FR2100517A1 (en) | 1971-04-21 | 1971-04-21 | Purification of lysozyme - by passage through a column of polyacrylam /agarose gel |
Country Status (1)
Country | Link |
---|---|
FR (1) | FR2100517A1 (en) |
Cited By (1)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
EP0180164A2 (en) * | 1984-10-26 | 1986-05-07 | Reanal Finomvegyszergyar | Method for the preparation of lysozyme enzyme |
-
1971
- 1971-04-21 FR FR7114089A patent/FR2100517A1/en active Granted
Cited By (2)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
EP0180164A2 (en) * | 1984-10-26 | 1986-05-07 | Reanal Finomvegyszergyar | Method for the preparation of lysozyme enzyme |
EP0180164A3 (en) * | 1984-10-26 | 1987-08-05 | Reanal Finomvegyszergyar | Method for the preparation of lysozyme enzyme |
Also Published As
Publication number | Publication date |
---|---|
FR2100517B1 (en) | 1973-06-08 |
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Legal Events
Date | Code | Title | Description |
---|---|---|---|
CL | Concession to grant licenses | ||
CD | Change of name or company name | ||
CD | Change of name or company name | ||
TP | Transmission of property | ||
ST | Notification of lapse |