FI951082A0 - An improved method for quantifying nucleic acid - Google Patents

An improved method for quantifying nucleic acid

Info

Publication number
FI951082A0
FI951082A0 FI951082A FI951082A FI951082A0 FI 951082 A0 FI951082 A0 FI 951082A0 FI 951082 A FI951082 A FI 951082A FI 951082 A FI951082 A FI 951082A FI 951082 A0 FI951082 A0 FI 951082A0
Authority
FI
Finland
Prior art keywords
nucleic acid
analyte
constructs
construct
improved method
Prior art date
Application number
FI951082A
Other languages
Finnish (fi)
Swedish (sv)
Other versions
FI951082A (en
FI112094B (en
Inventor
Marinus Gerardus Joh Beuningen
Tim Kievits
Thomas Augstinus Maria Beumer
Original Assignee
Akzo Nobel Nv
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Akzo Nobel Nv filed Critical Akzo Nobel Nv
Publication of FI951082A publication Critical patent/FI951082A/en
Publication of FI951082A0 publication Critical patent/FI951082A0/en
Application granted granted Critical
Publication of FI112094B publication Critical patent/FI112094B/en

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844—Nucleic acid amplification reactions
    • C12Q1/6865—Promoter-based amplification, e.g. nucleic acid sequence amplification [NASBA], self-sustained sequence replication [3SR] or transcription-based amplification system [TAS]
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844—Nucleic acid amplification reactions
    • C12Q1/6851—Quantitative amplification
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/70—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving virus or bacteriophage
    • C12Q1/701—Specific hybridization probes
    • C12Q1/702—Specific hybridization probes for retroviruses
    • C12Q1/703—Viruses associated with AIDS

Abstract

The present invention is related to an improved method for the quantification of nucleic acid, which can be performed with a minimal amount of nucleic acid amplification reactions. The method according to the invention for the quantification of analyte nucleic acid in a sample comprises the steps of: adding to the sample different respective amounts of different nucleic acid constructs, each construct being distinguishable from the analyte nucleic acid and capable of being co-amplified with the analyte nucleic acid; subjecting the sample to a nucleic acid amplification procedure, using amplification reagents capable of reacting with both the analyte nucleic acid and the nucleic acid constructs; detecting the relative amounts of amplificates derived from analyte nucleic acid and each nucleic acid construct; calculating the amount of analyte nucleic acid from said relative amounts. Each nucleic acid construct is different; the nucleic acid constructs can be distinguished from one another and from the analyte nuclic acid. The nucleic acid constructs do resemble each other, and the analyte nucleic acid, in that all are capable of reacting with the same amplification reagents.
FI951082A 1993-07-09 1995-03-08 An improved method for quantifying nucleic acid FI112094B (en)

Applications Claiming Priority (6)

Application Number Priority Date Filing Date Title
EP93202015 1993-07-09
EP93202015 1993-07-09
EP93203424 1993-12-06
EP93203424 1993-12-06
EP9402295 1994-07-08
PCT/EP1994/002295 WO1995002067A1 (en) 1993-07-09 1994-07-08 Improved method for the quantification of nucleic acid

Publications (3)

Publication Number Publication Date
FI951082A FI951082A (en) 1995-03-08
FI951082A0 true FI951082A0 (en) 1995-03-08
FI112094B FI112094B (en) 2003-10-31

Family

ID=26133903

Family Applications (1)

Application Number Title Priority Date Filing Date
FI951082A FI112094B (en) 1993-07-09 1995-03-08 An improved method for quantifying nucleic acid

Country Status (10)

Country Link
JP (1) JP3594598B2 (en)
KR (1) KR100312800B1 (en)
AT (1) ATE178946T1 (en)
AU (1) AU668659B2 (en)
CA (1) CA2143857C (en)
DE (1) DE69417863T2 (en)
DK (1) DK0662156T3 (en)
ES (1) ES2131699T3 (en)
FI (1) FI112094B (en)
WO (1) WO1995002067A1 (en)

Families Citing this family (22)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
DE69432419T2 (en) * 1993-09-22 2004-01-08 Igen International, Inc. AN ELECTROCHEMILUMINESCENT DETECTION OF NUCLEIC ACIDS BASED ON THE SELF-SUPPORTING SEQUENCE REPLICATION
CA2159044A1 (en) * 1994-09-26 1996-03-27 Falko-Guenter Falkner Method of quantitating nucleic acids
US5705365A (en) * 1995-06-07 1998-01-06 Gen-Probe Incorporated Kits for determining pre-amplification levels of a nucleic acid target sequence from post-amplification levels of product
US5710029A (en) * 1995-06-07 1998-01-20 Gen-Probe Incorporated Methods for determining pre-amplification levels of a nucleic acid target sequence from post-amplification levels of product
JP3996639B2 (en) * 1995-06-07 2007-10-24 ジェン−プローブ・インコーポレーテッド Method and kit for determining the pre-amplification level of a nucleic acid target sequence from the post-amplification level of a product
GB9519638D0 (en) * 1995-09-26 1995-11-29 Dynal As Method
US5795722A (en) * 1997-03-18 1998-08-18 Visible Genetics Inc. Method and kit for quantitation and nucleic acid sequencing of nucleic acid analytes in a sample
US5677124A (en) * 1996-07-03 1997-10-14 Ambion, Inc. Ribonuclease resistant viral RNA standards
US5939262A (en) 1996-07-03 1999-08-17 Ambion, Inc. Ribonuclease resistant RNA preparation and utilization
US6143496A (en) 1997-04-17 2000-11-07 Cytonix Corporation Method of sampling, amplifying and quantifying segment of nucleic acid, polymerase chain reaction assembly having nanoliter-sized sample chambers, and method of filling assembly
FR2765590B1 (en) * 1997-07-01 2002-08-09 Pasteur Institut SEMI-QUANTIFICATION METHOD OF WHEELS
AU4515699A (en) * 1998-06-26 2000-01-17 Akzo Nobel N.V. Tagging of rna amplicons generated by transcription-based amplification
EP1013776A1 (en) * 1998-12-22 2000-06-28 Universiteit van Amsterdam A sensitive assay for the detection or quantitation of human cytomegalovirus nucleic acid
GB9902422D0 (en) * 1999-02-03 1999-03-24 Lgc Teddington Limited Reference material for nucleic acid amplification
DE60022541T2 (en) * 1999-07-23 2006-06-14 Gen Probe Inc POLYNUCLEOTIDE AMPLIFICATION PROCESS
AT409383B (en) * 1999-12-22 2002-07-25 Baxter Ag METHOD FOR DETECTING AND QUANTIFYING NUCLEIC ACIDS IN A SAMPLE
WO2002024949A1 (en) * 2000-09-22 2002-03-28 Hitachi, Ltd. Method of analyzing nucleic acid
US6312929B1 (en) * 2000-12-22 2001-11-06 Cepheid Compositions and methods enabling a totally internally controlled amplification reaction
JP5564795B2 (en) * 2009-01-06 2014-08-06 株式会社島津製作所 DNA quantification method and gene analysis method
JP2011062119A (en) 2009-09-16 2011-03-31 Seiko Epson Corp Chip for quantitatively determining biological sample
KR20140006963A (en) * 2011-02-25 2014-01-16 노파르티스 아게 Exogenous internal positive control
KR102108855B1 (en) 2017-10-30 2020-05-12 한국표준과학연구원 Method for quantification of nucleic acids wherein stable isotope-labelled nucleic acids are used as internal standards and uses thereof

Family Cites Families (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4942124A (en) * 1987-08-11 1990-07-17 President And Fellows Of Harvard College Multiplex sequencing
DE69233285T2 (en) * 1991-08-02 2004-11-25 bioMérieux B.V. Quantification of nucleic acids
FR2683827B1 (en) * 1991-11-15 1994-03-04 Institut Nal Sante Recherc Medic METHOD FOR DETERMINING THE QUANTITY OF A FRAGMENT OF DNA OF INTEREST BY AN ENZYMATIC AMPLIFICATION METHOD.

Also Published As

Publication number Publication date
AU668659B2 (en) 1996-05-09
JPH08501222A (en) 1996-02-13
DK0662156T3 (en) 1999-10-25
FI951082A (en) 1995-03-08
CA2143857A1 (en) 1995-01-19
CA2143857C (en) 2005-09-20
KR100312800B1 (en) 2002-02-28
FI112094B (en) 2003-10-31
JP3594598B2 (en) 2004-12-02
ES2131699T3 (en) 1999-08-01
AU7492894A (en) 1995-02-06
DE69417863T2 (en) 1999-10-07
ATE178946T1 (en) 1999-04-15
DE69417863D1 (en) 1999-05-20
WO1995002067A1 (en) 1995-01-19

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