FI873153A0 - Process for Preparation of Human Antithrombin III (ATIII) in a Mammalian Cell Culture and Useful Vectors and Mammalian Host Cells - Google Patents
Process for Preparation of Human Antithrombin III (ATIII) in a Mammalian Cell Culture and Useful Vectors and Mammalian Host CellsInfo
- Publication number
- FI873153A0 FI873153A0 FI873153A FI873153A FI873153A0 FI 873153 A0 FI873153 A0 FI 873153A0 FI 873153 A FI873153 A FI 873153A FI 873153 A FI873153 A FI 873153A FI 873153 A0 FI873153 A0 FI 873153A0
- Authority
- FI
- Finland
- Prior art keywords
- atiii
- antithrombin iii
- preparation
- cell culture
- host cells
- Prior art date
Links
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
- C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
- C12N15/85—Vectors or expression systems specially adapted for eukaryotic hosts for animal cells
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
- C07K14/81—Protease inhibitors
- C07K14/8107—Endopeptidase (E.C. 3.4.21-99) inhibitors
- C07K14/811—Serine protease (E.C. 3.4.21) inhibitors
- C07K14/8121—Serpins
- C07K14/8128—Antithrombin III
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N2800/00—Nucleic acids vectors
- C12N2800/10—Plasmid DNA
- C12N2800/108—Plasmid DNA episomal vectors
Abstract
Human antithrombin III (ATIII) which has progressive inhibitory activity and is stimulated by heparin is advantageously obtained by expression after cotransfection of mammalian cells whose dihydrofolate reductase (DHFR) activity is deficient (dhfr<->) with a) vectors which contain the ATIII cDNA and b) vectors which harbour the DHFR gene, especially after gene amplification with methotrexate.
Applications Claiming Priority (2)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
DE3624453 | 1986-07-19 | ||
DE19863624453 DE3624453A1 (en) | 1986-07-19 | 1986-07-19 | METHOD FOR THE PRODUCTION OF HUMAN ANTITHROMBIN III (ATIII), VECTORS AND Host CELLS SUITABLE FOR THEM, BIOLOGICALLY ACTIVE ATIII, AND THE MEDICINAL PRODUCT CONTAINING THEM |
Publications (4)
Publication Number | Publication Date |
---|---|
FI873153A0 true FI873153A0 (en) | 1987-07-16 |
FI873153A FI873153A (en) | 1988-01-20 |
FI94425B FI94425B (en) | 1995-05-31 |
FI94425C FI94425C (en) | 1995-09-11 |
Family
ID=6305549
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
FI873153A FI94425C (en) | 1986-07-19 | 1987-07-16 | Method for the production of human antithrombin III (ATIII) in mammalian cell culture and suitable vectors and mammalian host cells |
Country Status (11)
Country | Link |
---|---|
EP (1) | EP0256302B1 (en) |
JP (2) | JP2721158B2 (en) |
AT (1) | ATE85348T1 (en) |
AU (1) | AU610830B2 (en) |
CA (1) | CA1305932C (en) |
DE (2) | DE3624453A1 (en) |
DK (1) | DK174993B1 (en) |
ES (1) | ES2053476T3 (en) |
FI (1) | FI94425C (en) |
GR (1) | GR3007700T3 (en) |
PT (1) | PT85346B (en) |
Families Citing this family (3)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
JPH03215430A (en) * | 1990-01-19 | 1991-09-20 | Kita Kiyoshi | Anti-coagulant for articular cavity |
DE3901917A1 (en) * | 1989-01-24 | 1990-07-26 | Behringwerke Ag | MUTANTS OF HUMAN ANTITHROMBIN III |
MXPA04005191A (en) * | 2001-11-28 | 2005-04-29 | Sandoz Ag | Method for producing a recombinant polypeptide. |
Family Cites Families (7)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
JPS597693B2 (en) * | 1978-01-07 | 1984-02-20 | 株式会社ミドリ十字 | Antithrombin preparation and its manufacturing method |
JPS5846100A (en) * | 1981-09-11 | 1983-03-17 | Agency Of Ind Science & Technol | Plasmid vector having three kinds of marker and its preparation |
GB2116183B (en) * | 1982-03-03 | 1985-06-05 | Genentech Inc | Human antithrombin iii dna sequences therefore expression vehicles and cloning vectors containing such sequences and cell cultures transformed thereby a process for expressing human antithrombin iii and pharmaceutical compositions comprising it |
DE3382068D1 (en) * | 1982-03-15 | 1991-01-31 | Univ Columbia | METHOD FOR INTRODUCING CLONED AMPLIFICABLE GENES IN EUKARYOTIC CELLS AND FOR THE PRODUCTION OF PROTEIN PRODUCTS. |
IL73854A0 (en) * | 1983-12-27 | 1985-03-31 | Genetics Inst | Vectors containing accessory dna for transformation of eucaryotic cells |
IL71691A (en) * | 1984-04-27 | 1991-04-15 | Yeda Res & Dev | Production of interferon-ypsilon |
US4656254A (en) * | 1985-12-02 | 1987-04-07 | Miles Laboratories, Inc. | Method of preparing alpha-1-proteinase inhibitor and antithrombin III |
-
1986
- 1986-07-19 DE DE19863624453 patent/DE3624453A1/en not_active Withdrawn
-
1987
- 1987-07-11 ES ES87110027T patent/ES2053476T3/en not_active Expired - Lifetime
- 1987-07-11 DE DE8787110027T patent/DE3783968D1/en not_active Expired - Lifetime
- 1987-07-11 AT AT87110027T patent/ATE85348T1/en not_active IP Right Cessation
- 1987-07-11 EP EP87110027A patent/EP0256302B1/en not_active Expired - Lifetime
- 1987-07-16 PT PT85346A patent/PT85346B/en unknown
- 1987-07-16 FI FI873153A patent/FI94425C/en not_active IP Right Cessation
- 1987-07-17 CA CA000542428A patent/CA1305932C/en not_active Expired - Lifetime
- 1987-07-17 AU AU75773/87A patent/AU610830B2/en not_active Expired
- 1987-07-17 DK DK198703751A patent/DK174993B1/en not_active IP Right Cessation
- 1987-07-18 JP JP62179879A patent/JP2721158B2/en not_active Expired - Lifetime
-
1993
- 1993-04-27 GR GR930400430T patent/GR3007700T3/el unknown
-
1996
- 1996-10-16 JP JP8273823A patent/JP2798659B2/en not_active Expired - Lifetime
Also Published As
Publication number | Publication date |
---|---|
FI94425B (en) | 1995-05-31 |
ES2053476T3 (en) | 1994-08-01 |
FI873153A (en) | 1988-01-20 |
ATE85348T1 (en) | 1993-02-15 |
EP0256302A2 (en) | 1988-02-24 |
AU7577387A (en) | 1988-03-03 |
CA1305932C (en) | 1992-08-04 |
DE3624453A1 (en) | 1988-01-28 |
AU610830B2 (en) | 1991-05-30 |
JP2798659B2 (en) | 1998-09-17 |
JPH09117293A (en) | 1997-05-06 |
EP0256302A3 (en) | 1989-11-02 |
JP2721158B2 (en) | 1998-03-04 |
DK375187A (en) | 1988-01-20 |
PT85346B (en) | 1990-06-29 |
DK174993B1 (en) | 2004-04-13 |
PT85346A (en) | 1987-08-01 |
JPS6344898A (en) | 1988-02-25 |
GR3007700T3 (en) | 1993-08-31 |
EP0256302B1 (en) | 1993-02-03 |
DK375187D0 (en) | 1987-07-17 |
FI94425C (en) | 1995-09-11 |
DE3783968D1 (en) | 1993-03-18 |
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Legal Events
Date | Code | Title | Description |
---|---|---|---|
BB | Publication of examined application | ||
FG | Patent granted |
Owner name: BEHRINGWERKE AKTIENGESELLSCHAFT |
|
MA | Patent expired |