ES8802262A1 - Procedimiento para la obtencion de un anticuerpo monoclonal contra la hormona luteinizante - Google Patents
Procedimiento para la obtencion de un anticuerpo monoclonal contra la hormona luteinizanteInfo
- Publication number
- ES8802262A1 ES8802262A1 ES552704A ES552704A ES8802262A1 ES 8802262 A1 ES8802262 A1 ES 8802262A1 ES 552704 A ES552704 A ES 552704A ES 552704 A ES552704 A ES 552704A ES 8802262 A1 ES8802262 A1 ES 8802262A1
- Authority
- ES
- Spain
- Prior art keywords
- hormone
- reagent
- determination
- well
- appropriate monoclonal
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Expired
Links
Classifications
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K16/00—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies
- C07K16/18—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies against material from animals or humans
- C07K16/26—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies against material from animals or humans against hormones ; against hormone releasing or inhibiting factors
Landscapes
- Health & Medical Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Immunology (AREA)
- Molecular Biology (AREA)
- Organic Chemistry (AREA)
- Biochemistry (AREA)
- Engineering & Computer Science (AREA)
- General Health & Medical Sciences (AREA)
- Medicinal Chemistry (AREA)
- Biomedical Technology (AREA)
- Hematology (AREA)
- Genetics & Genomics (AREA)
- Biophysics (AREA)
- Endocrinology (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Urology & Nephrology (AREA)
- Physics & Mathematics (AREA)
- Pathology (AREA)
- Cell Biology (AREA)
- Food Science & Technology (AREA)
- Biotechnology (AREA)
- Analytical Chemistry (AREA)
- General Physics & Mathematics (AREA)
- Microbiology (AREA)
- Preparation Of Compounds By Using Micro-Organisms (AREA)
- Peptides Or Proteins (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Investigating Or Analysing Biological Materials (AREA)
- Organic Low-Molecular-Weight Compounds And Preparation Thereof (AREA)
- Medicines Containing Antibodies Or Antigens For Use As Internal Diagnostic Agents (AREA)
Abstract
PROCEDIMIENTO PARA LA DETERMINACION DE LA HORMONA LUTEINIZANTE (LH) MEDIANTE LA OBTENCION PREVIA DE UN ANTICUERPO MONOCLONAL. COMPRENDE LAS SIGUIENTES ETAPAS: A) INMUNIZAR ANIMALES APROPIADOS (POR EJEMPLO RATONES) POR LO MENOS CUATRO VECES, CADA VEZ CON 50 A 200 MG DE LH CON UN INTERVALO DE TIEMPO DE 4 A 6 SEMANAS, UTILIZANDOSE COMO COADYUVANTE HIDROXIDO DE ALUMINIO Y BORDETELLA PERTUSSIO, REALIZANDOSE UNA NUEVA INMUNIZACION CON LAS MISMAS CANTIDADES ALGUNOS DIAS ANTES DEL FUSIONAMIENTO; B) RECUPERAR LINFOTICOS-B A PARTIR DEL BAZO DE LOS ANIMALES INMUNIZADOS; C) FUSIONARLOS CON CELULAS DE MIELOMA; D) SELECCIONAR LAS CELULAS DE HIBRIDOMA FORMADAS SOBRE UN MEDIO DE SELECCION CON HIPOXANTINO Y AZASERINA; E) CLONAR ADICIONALMENTE CULTIVOS QUE CONTIENEN ANTICUERPOS DIRIGIDOS CONTRA LA LH; Y F) RECUPERAR LOS ANTICUERPOS FORMADOS POR LOS CLONES. DE APLICACION EN ANALISIS DE LIQUIDOS CORPORALES PARA EVALUAR EL ESTADO ENDOCRINOLOGICO DE HIPOTALAMO, LA HIPOFISIS Y LAS GONADAS.
Applications Claiming Priority (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
DE19853507848 DE3507848A1 (de) | 1985-03-06 | 1985-03-06 | Verfahren und reagenz zur bestimmung des luteinisierenden hormons sowie hierzu geeignete monoklonale antikoerper |
Publications (2)
Publication Number | Publication Date |
---|---|
ES552704A0 ES552704A0 (es) | 1988-05-01 |
ES8802262A1 true ES8802262A1 (es) | 1988-05-01 |
Family
ID=6264312
Family Applications (2)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
ES552704A Expired ES8802262A1 (es) | 1985-03-06 | 1986-03-06 | Procedimiento para la obtencion de un anticuerpo monoclonal contra la hormona luteinizante |
ES552706A Expired ES8802263A1 (es) | 1985-03-06 | 1986-03-06 | Procedimiento inmunologico para la determinacion de la hormona luteinizante |
Family Applications After (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
ES552706A Expired ES8802263A1 (es) | 1985-03-06 | 1986-03-06 | Procedimiento inmunologico para la determinacion de la hormona luteinizante |
Country Status (10)
Country | Link |
---|---|
EP (1) | EP0193881B1 (es) |
JP (1) | JPS61218599A (es) |
KR (1) | KR890003591B1 (es) |
AT (1) | ATE142023T1 (es) |
AU (1) | AU561726B2 (es) |
CA (1) | CA1273306A (es) |
DE (2) | DE3507848A1 (es) |
ES (2) | ES8802262A1 (es) |
FI (1) | FI91975C (es) |
ZA (1) | ZA861550B (es) |
Families Citing this family (4)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US5622871A (en) | 1987-04-27 | 1997-04-22 | Unilever Patent Holdings B.V. | Capillary immunoassay and device therefor comprising mobilizable particulate labelled reagents |
EP1555529A3 (en) | 1987-04-27 | 2008-06-04 | Inverness Medical Switzerland GmbH | Immunochromatographic specific binding assay device and method. |
US6352862B1 (en) | 1989-02-17 | 2002-03-05 | Unilever Patent Holdings B.V. | Analytical test device for imuno assays and methods of using same |
FR2662804B1 (fr) * | 1990-06-01 | 1994-05-27 | Agronomique Inst Nat Rech | Anticorps polyclonaux anti-lh et leurs applications a la detection et/ou au dosage de la lh, chez de multiples especes animales. |
Family Cites Families (4)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
DE3114362C2 (de) * | 1980-04-11 | 1983-08-18 | Toshiyuki Prof. Nara Hamaoka | Verfahren zur Herstellung eines Antikörpers bzw. Antiserums, Verwendung dieses Verfahrens zur Gewinnung von Säugetierzellen, welche fähig sind, einen solchen Antikörper zu erzeugen und Verwendung des so hergestellten Antikörpers bzw. Antiserums zur Immunoprüfung |
GB2111201A (en) * | 1981-12-10 | 1983-06-29 | Celltech Ltd | Immunoassay of human luteinising hormone |
JP2538646B2 (ja) * | 1988-07-05 | 1996-09-25 | 花王株式会社 | 新規カチオン化合物、それを含有する漂白剤組成物及び漂白洗浄剤組成物 |
JPH0256080A (ja) * | 1988-08-22 | 1990-02-26 | Fuji Electric Co Ltd | バーコードリーダ |
-
1985
- 1985-03-06 DE DE19853507848 patent/DE3507848A1/de not_active Ceased
-
1986
- 1986-02-27 CA CA000502915A patent/CA1273306A/en not_active Expired - Lifetime
- 1986-02-27 AU AU54130/86A patent/AU561726B2/en not_active Ceased
- 1986-02-28 EP EP86102590A patent/EP0193881B1/de not_active Expired - Lifetime
- 1986-02-28 AT AT86102590T patent/ATE142023T1/de not_active IP Right Cessation
- 1986-02-28 DE DE3650561T patent/DE3650561D1/de not_active Expired - Lifetime
- 1986-03-03 ZA ZA861550A patent/ZA861550B/xx unknown
- 1986-03-05 FI FI860936A patent/FI91975C/fi not_active IP Right Cessation
- 1986-03-05 KR KR1019860001523A patent/KR890003591B1/ko not_active IP Right Cessation
- 1986-03-06 ES ES552704A patent/ES8802262A1/es not_active Expired
- 1986-03-06 ES ES552706A patent/ES8802263A1/es not_active Expired
- 1986-03-06 JP JP61047477A patent/JPS61218599A/ja active Granted
Also Published As
Publication number | Publication date |
---|---|
ES552706A0 (es) | 1988-05-01 |
EP0193881A2 (de) | 1986-09-10 |
AU5413086A (en) | 1986-10-02 |
ES552704A0 (es) | 1988-05-01 |
DE3507848A1 (de) | 1986-11-13 |
CA1273306A (en) | 1990-08-28 |
ES8802263A1 (es) | 1988-05-01 |
JPS61218599A (ja) | 1986-09-29 |
FI860936A (fi) | 1986-09-07 |
FI860936A0 (fi) | 1986-03-05 |
KR860007547A (ko) | 1986-10-13 |
FI91975B (fi) | 1994-05-31 |
ATE142023T1 (de) | 1996-09-15 |
EP0193881B1 (de) | 1996-08-28 |
ZA861550B (en) | 1986-11-26 |
DE3650561D1 (de) | 1996-10-02 |
EP0193881A3 (en) | 1988-11-09 |
FI91975C (fi) | 1994-09-12 |
JPH0471518B2 (es) | 1992-11-13 |
KR890003591B1 (ko) | 1989-09-25 |
AU561726B2 (en) | 1987-05-14 |
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Legal Events
Date | Code | Title | Description |
---|---|---|---|
FD1A | Patent lapsed |
Effective date: 20080318 |