ES8801551A1 - Un procedimiento para preparar un vector de expresion replicable. - Google Patents
Un procedimiento para preparar un vector de expresion replicable.Info
- Publication number
- ES8801551A1 ES8801551A1 ES557675A ES557675A ES8801551A1 ES 8801551 A1 ES8801551 A1 ES 8801551A1 ES 557675 A ES557675 A ES 557675A ES 557675 A ES557675 A ES 557675A ES 8801551 A1 ES8801551 A1 ES 8801551A1
- Authority
- ES
- Spain
- Prior art keywords
- fibrinolytic enzyme
- modified
- region
- growth factor
- plasminogen activator
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Expired
Links
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
- C12N9/14—Hydrolases (3)
- C12N9/48—Hydrolases (3) acting on peptide bonds (3.4)
- C12N9/50—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25)
- C12N9/64—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from animal tissue
- C12N9/6421—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from animal tissue from mammals
- C12N9/6424—Serine endopeptidases (3.4.21)
- C12N9/6456—Plasminogen activators
- C12N9/6459—Plasminogen activators t-plasminogen activator (3.4.21.68), i.e. tPA
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Y—ENZYMES
- C12Y304/00—Hydrolases acting on peptide bonds, i.e. peptidases (3.4)
- C12Y304/21—Serine endopeptidases (3.4.21)
- C12Y304/21069—Protein C activated (3.4.21.69)
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K38/00—Medicinal preparations containing peptides
Landscapes
- Health & Medical Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Engineering & Computer Science (AREA)
- Organic Chemistry (AREA)
- Life Sciences & Earth Sciences (AREA)
- Zoology (AREA)
- Genetics & Genomics (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Wood Science & Technology (AREA)
- General Health & Medical Sciences (AREA)
- Biochemistry (AREA)
- General Engineering & Computer Science (AREA)
- Biomedical Technology (AREA)
- Microbiology (AREA)
- Biotechnology (AREA)
- Medicinal Chemistry (AREA)
- Molecular Biology (AREA)
- Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)
- Enzymes And Modification Thereof (AREA)
Abstract
METODO DE PREPARACION DE VECTORES DE EXPRESION REPLICABLES. CONSISTE EN ESCINDIR UN VECTOR COMPATIBLE CON UNA CELULA HUESPED, CON UN ENZIMA DE RESTRICCION PARA OBTENER UN SEGMENTO DE ADN LINEAL QUE TIENE UN REPLICON INTACTO; Y COMBINAR EN CONDICIONES DE LIGACION EN PRESENCIA DE UNA LIGASA EL SEGMENTO LINEAL ASI FORMADO CON UN POLIMERO DE ADN QUE CONTIENE UNA SECUENCIA DE NUCLEOTIDOS QUE CODIFICA UN ACTIVANTE DE PLASMINOGENO DE TIPO TEJIDO MODIFICADO EN LA REGION DEL DOMINIO DEL FACTOR DE CRECIMIENTO. ESTOS COMPUESTOS TIENEN APLICACIONES FARMACOLOGICAS PARA EL TRATAMIENTO DE ENFERMEDADES TROMBOTICAS.
Applications Claiming Priority (2)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
GB858508972A GB8508972D0 (en) | 1985-04-04 | 1985-04-04 | Compounds |
GB868604852A GB8604852D0 (en) | 1986-02-27 | 1986-02-27 | Compounds |
Publications (2)
Publication Number | Publication Date |
---|---|
ES8801551A1 true ES8801551A1 (es) | 1987-12-16 |
ES557675A0 ES557675A0 (es) | 1987-12-16 |
Family
ID=26289098
Family Applications (5)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
ES553651A Expired ES8801374A1 (es) | 1985-04-04 | 1986-04-02 | Un procedimiento para preparar un activante de plasminogeno. |
ES557675A Expired ES8801551A1 (es) | 1985-04-04 | 1987-08-17 | Un procedimiento para preparar un vector de expresion replicable. |
ES557674A Expired ES8802442A1 (es) | 1985-04-04 | 1987-08-17 | Un procedimiento para preparar un polimero de adn. |
ES557677A Expired ES8801552A1 (es) | 1985-04-04 | 1987-08-17 | Un procedimiento para preparar una proteina hibrida fibrinoliticamente activa. |
ES557676A Expired ES8802537A1 (es) | 1985-04-04 | 1987-08-17 | Un procedimiento para preparar una celula huesped con un vector de expresion correspondiente a un activante de plasminogenos de tipo tejido modificado. |
Family Applications Before (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
ES553651A Expired ES8801374A1 (es) | 1985-04-04 | 1986-04-02 | Un procedimiento para preparar un activante de plasminogeno. |
Family Applications After (3)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
ES557674A Expired ES8802442A1 (es) | 1985-04-04 | 1987-08-17 | Un procedimiento para preparar un polimero de adn. |
ES557677A Expired ES8801552A1 (es) | 1985-04-04 | 1987-08-17 | Un procedimiento para preparar una proteina hibrida fibrinoliticamente activa. |
ES557676A Expired ES8802537A1 (es) | 1985-04-04 | 1987-08-17 | Un procedimiento para preparar una celula huesped con un vector de expresion correspondiente a un activante de plasminogenos de tipo tejido modificado. |
Country Status (6)
Country | Link |
---|---|
EP (1) | EP0207589A1 (es) |
DK (1) | DK149786A (es) |
ES (5) | ES8801374A1 (es) |
GR (1) | GR860875B (es) |
NZ (1) | NZ215660A (es) |
PT (1) | PT82326B (es) |
Families Citing this family (33)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
ATE85081T1 (de) * | 1985-12-20 | 1993-02-15 | Upjohn Co | Analoge des gewebespezifischen plasminogenaktivators. |
US5106741A (en) * | 1985-12-20 | 1992-04-21 | The Upjohn Company | Tissue plasminogen activator (TPA) analogs |
US5002887A (en) * | 1986-01-31 | 1991-03-26 | Genetics Institute, Inc. | Truncated thrombolytic proteins |
US5258298A (en) * | 1986-01-31 | 1993-11-02 | Genetics Institute, Inc. | Deletion and glycosylation mutant of human tissue plasminogen activator |
US5837518A (en) * | 1986-01-31 | 1998-11-17 | Genetics Institute, Inc. | Thrombolytic proteins |
EP0234051B1 (en) * | 1986-02-17 | 1991-12-11 | Stichting Centraal Laboratorium van de Bloedtransfusiedienst van het Nederlandse Rode Kruis | Tissue-type plasminogen activator mutants; recombinant genetic information coding therefor and process for preparing said mutants; their use and pharmaceutical compositions |
AU7090887A (en) * | 1986-04-02 | 1987-10-08 | Beecham Group Plc | Plasminogen activators modified in first kringle domain |
PT84589B (en) * | 1986-04-02 | 1989-05-09 | Beecham Group Plc | Process for preparing a fibrinolytic enzyme |
PT84587B (pt) * | 1986-04-02 | 1989-11-30 | Beecham Group Plc | Processo para preparacao de uma enzima fibrinolitica |
DE3643158A1 (de) * | 1986-04-21 | 1987-11-19 | Boehringer Mannheim Gmbh | Gewebs-plasminogen-aktivator(tpa) - derivat und seine herstellung |
EP0266032A1 (en) * | 1986-08-29 | 1988-05-04 | Beecham Group Plc | Modified fibrinolytic enzyme |
US5580559A (en) * | 1986-12-05 | 1996-12-03 | Ciba-Geigy Corporation | Hybrid plasminogen activator |
US5242819A (en) * | 1986-12-05 | 1993-09-07 | Ciba-Geigy Corporation | DNA molecules encoding hybrid proteins of tissue plasminogen activator and urokinase |
GB8701811D0 (en) * | 1987-01-28 | 1987-03-04 | Beecham Group Plc | Compounds |
WO1988005822A1 (en) * | 1987-01-30 | 1988-08-11 | American Home Products Corporation | Des-epidermal growth factor plasminogen activators |
NL8701021A (nl) * | 1987-04-29 | 1988-11-16 | Stichting Centraal Lab | Humane t-pa(u-pa) substitutie-mutant eiwitten, daarvoor coderend recombinant dna, getransfecteerde gastheercellen, bereiding van de mutant eiwitten, en farmaceutische preparaten. |
DK302388A (da) * | 1987-06-04 | 1989-02-03 | Zymogenetics Inc | T-pa analoger med dodificeret vaekstfaktordomaene |
IE64782B1 (en) * | 1987-06-04 | 1995-09-06 | Eisai Co Ltd | Mutant t-PA with kringle replacement |
SE8702562L (sv) * | 1987-06-18 | 1988-12-19 | Kabigen Ab | Nya fibrinolytiska enzymer |
JPH02504102A (ja) * | 1987-06-30 | 1990-11-29 | ジェネンテク,インコーポレイテッド | 血管障害治療のための改良法 |
AU613942B2 (en) * | 1987-06-30 | 1991-08-15 | Genentech Inc. | Improved processes for the treatment of vascular disease |
US4935237A (en) * | 1988-03-21 | 1990-06-19 | Genentech, Inc. | Processes for the preparation of t-PA mutants |
AU2081788A (en) * | 1987-07-06 | 1989-01-30 | Genetics Institute Inc. | Novel thrombolytic proteins |
GB8721023D0 (en) * | 1987-09-07 | 1987-10-14 | Lepetit Spa | Modified plasminogen activators |
US5100666A (en) * | 1987-10-09 | 1992-03-31 | Monsanto Company | Modified tissue plasminogen activator K2K2SP |
US5244676A (en) * | 1987-10-09 | 1993-09-14 | Monsanto Company | Modified tissue plasminogen activator with modified glycosylation site |
US5037646A (en) * | 1988-04-29 | 1991-08-06 | Genentech, Inc. | Processes for the treatment of vascular disease |
US5346824A (en) * | 1988-05-20 | 1994-09-13 | Genentech, Inc. | DNA encoding variants of tissue plasminogen activators and expression vectors and hosts thereof |
US5270198A (en) * | 1988-05-20 | 1993-12-14 | Genentech, Inc. | DNA molecules encoding variants of tissue plasminogen activators, vectors, and host cells |
US5262170A (en) * | 1988-09-02 | 1993-11-16 | Genentech, Inc. | Tissue plasminogen activator having zymogenic or fibrin specific properties and substituted at amino acid positions 296-299, DNA molecules encoding them, vectors, and host cells |
US5714145A (en) * | 1988-09-02 | 1998-02-03 | Genentech, Inc. | Tissue plasminogen activator having zymogenic or fibrin specific properties |
DE10199044I2 (de) * | 1992-06-03 | 2006-02-09 | Genentech Inc | Varianten des Gewebeplasminogenaktivators mit verbesserter Therapeutischer wirkung |
GB9614871D0 (en) | 1996-07-15 | 1996-09-04 | Smithkline Beecham Plc | Compounds |
Family Cites Families (2)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
IL68561A (en) * | 1982-05-05 | 1991-01-31 | Genentech Inc | Preparation of polypeptide with human tissue plasminogen activator function,processes for making it,and dna and transformed cell intermediates thereof |
IE56716B1 (en) * | 1983-01-19 | 1991-11-20 | Genentech Inc | Method for producing human tpa and expression vectors therefor |
-
1986
- 1986-04-02 ES ES553651A patent/ES8801374A1/es not_active Expired
- 1986-04-02 NZ NZ21566086A patent/NZ215660A/xx unknown
- 1986-04-02 EP EP19860302425 patent/EP0207589A1/en not_active Ceased
- 1986-04-02 GR GR860875A patent/GR860875B/el unknown
- 1986-04-02 PT PT8232686A patent/PT82326B/pt not_active IP Right Cessation
- 1986-04-02 DK DK149786A patent/DK149786A/da not_active Application Discontinuation
-
1987
- 1987-08-17 ES ES557675A patent/ES8801551A1/es not_active Expired
- 1987-08-17 ES ES557674A patent/ES8802442A1/es not_active Expired
- 1987-08-17 ES ES557677A patent/ES8801552A1/es not_active Expired
- 1987-08-17 ES ES557676A patent/ES8802537A1/es not_active Expired
Also Published As
Publication number | Publication date |
---|---|
ES553651A0 (es) | 1988-01-01 |
AU5551486A (en) | 1986-10-09 |
NZ215660A (en) | 1990-03-27 |
EP0207589A1 (en) | 1987-01-07 |
DK149786A (da) | 1986-10-04 |
ES557677A0 (es) | 1987-12-16 |
ES557676A0 (es) | 1988-07-16 |
ES8801552A1 (es) | 1987-12-16 |
ES8802442A1 (es) | 1988-06-01 |
AU596093B2 (en) | 1990-04-26 |
ES8801374A1 (es) | 1988-01-01 |
DK149786D0 (da) | 1986-04-02 |
PT82326B (pt) | 1988-10-14 |
ES557674A0 (es) | 1988-06-01 |
PT82326A (en) | 1986-05-01 |
GR860875B (en) | 1986-07-29 |
ES8802537A1 (es) | 1988-07-16 |
ES557675A0 (es) | 1987-12-16 |
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Legal Events
Date | Code | Title | Description |
---|---|---|---|
FD1A | Patent lapsed |
Effective date: 19960603 |