ES8800725A1 - Un procedimiento para producir un anticuerpo monoclonal - Google Patents

Un procedimiento para producir un anticuerpo monoclonal

Info

Publication number
ES8800725A1
ES8800725A1 ES551704A ES551704A ES8800725A1 ES 8800725 A1 ES8800725 A1 ES 8800725A1 ES 551704 A ES551704 A ES 551704A ES 551704 A ES551704 A ES 551704A ES 8800725 A1 ES8800725 A1 ES 8800725A1
Authority
ES
Spain
Prior art keywords
plasminogen activator
monoclonal antibodies
tissue plasminogen
human normal
normal cells
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired
Application number
ES551704A
Other languages
English (en)
Other versions
ES551704A0 (es
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Kowa Co Ltd
Original Assignee
Kowa Co Ltd
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Priority claimed from JP60020983A external-priority patent/JP2610808B2/ja
Priority claimed from JP60021862A external-priority patent/JPS61183299A/ja
Application filed by Kowa Co Ltd filed Critical Kowa Co Ltd
Publication of ES551704A0 publication Critical patent/ES551704A0/es
Publication of ES8800725A1 publication Critical patent/ES8800725A1/es
Expired legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/48—Hydrolases (3) acting on peptide bonds (3.4)
    • C12N9/50—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25)
    • C12N9/64—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from animal tissue
    • C12N9/6421—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from animal tissue from mammals
    • C12N9/6424—Serine endopeptidases (3.4.21)
    • C12N9/6456—Plasminogen activators
    • C12N9/6459—Plasminogen activators t-plasminogen activator (3.4.21.68), i.e. tPA
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K16/00—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies
    • C07K16/40—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies against enzymes
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y304/00—Hydrolases acting on peptide bonds, i.e. peptidases (3.4)
    • C12Y304/21—Serine endopeptidases (3.4.21)
    • C12Y304/21069—Protein C activated (3.4.21.69)

Landscapes

  • Chemical & Material Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Organic Chemistry (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Engineering & Computer Science (AREA)
  • Wood Science & Technology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biochemistry (AREA)
  • Zoology (AREA)
  • General Health & Medical Sciences (AREA)
  • Molecular Biology (AREA)
  • General Engineering & Computer Science (AREA)
  • Biomedical Technology (AREA)
  • Medicinal Chemistry (AREA)
  • Biophysics (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Immunology (AREA)
  • Biotechnology (AREA)
  • Microbiology (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Peptides Or Proteins (AREA)

Abstract

METODO DE PRODUCIR UN ANTICUERPO MONOCLONAL. CONSISTE EN: A) HABILITAR UNA CELULA HIBRIDA DE CELULAS DE MIELOMA DE RATONES Y ESPLENOCITOS DE RATONES INMUNIZADOS CON EL ACTIVADOR DE PLASMINOGENO DE TEJIDO, SIENDO LA CELULA HIBRIDA EL HIBRIDOMA X-21 QUE TIENE IFO 50086; B) CLONAR LA CELULA HIBRIDA PARA OBTENER CELULAS HIBRIDAS PRODUCTORAS DE ANTICUERPOS MONOCLONALES; C) CULTIVAR EN UN MEDIO DE CULTIVO LAS CELULAS HIBRIDAS PRODUCTORAS DE ANTICUERPOS MONOCLONALES; Y D) RECOGER UN ANTICUERPO MONOCLONAL SEPARANDOLO DE UN LIQUIDO SOBRENADANTE CONTENIDO EN EL MEDIO DE CULTIVO. TIENE UTILIDAD PARA PREPARAR COMPUESTOS FARMACEUTICOS.
ES551704A 1985-02-07 1986-02-06 Un procedimiento para producir un anticuerpo monoclonal Expired ES8800725A1 (es)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
JP60020983A JP2610808B2 (ja) 1985-02-07 1985-02-07 人正常細胞由来の組織型プラスミノーゲンアクチベーターに対するモノクロナル抗体を用いる免疫学的測定試薬
JP60021862A JPS61183299A (ja) 1985-02-08 1985-02-08 人正常細胞由来の組織型プラスミノ−ゲンアクチベ−タ−に対する新規なモノクロナル抗体およびそれによる精製法ならびに検出法

Publications (2)

Publication Number Publication Date
ES551704A0 ES551704A0 (es) 1987-11-16
ES8800725A1 true ES8800725A1 (es) 1987-11-16

Family

ID=26357991

Family Applications (2)

Application Number Title Priority Date Filing Date
ES551704A Expired ES8800725A1 (es) 1985-02-07 1986-02-06 Un procedimiento para producir un anticuerpo monoclonal
ES557082A Expired ES8707565A1 (es) 1985-02-07 1986-09-26 Un procedimiento para producir un anticuerpo monoclonal

Family Applications After (1)

Application Number Title Priority Date Filing Date
ES557082A Expired ES8707565A1 (es) 1985-02-07 1986-09-26 Un procedimiento para producir un anticuerpo monoclonal

Country Status (4)

Country Link
EP (1) EP0190711B1 (es)
CA (1) CA1293459C (es)
DE (1) DE3663030D1 (es)
ES (2) ES8800725A1 (es)

Families Citing this family (10)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JPS61221128A (ja) * 1985-03-26 1986-10-01 Snow Brand Milk Prod Co Ltd プラスミノ−ゲン活性化因子に対するモノクロ−ナル抗体とその調製方法及び該モノクロ−ナル抗体の使用方法
US5041381A (en) 1986-07-03 1991-08-20 Schering Corporation Monoclonal antibodies against human interleukin-4 and hybridomas producing the same
US4833085A (en) * 1986-08-13 1989-05-23 Monsanto Company Monoclonal antibody specific for human colon fibroblast-derived t-PA
FI89634C (fi) * 1987-07-10 1993-10-25 Yamanouchi Pharma Co Ltd Foerfarande, anordning och kitt foer maetning av vaevnadsplasminogenaktivator-aktivitet
US5411864A (en) * 1987-11-05 1995-05-02 Genentech, Inc. Method of purifying recombinant proteins from corresponding host cell proteins
US5225540A (en) * 1988-04-26 1993-07-06 Du Pont Merck Pharmaceutical Company Monoclonal antibodies to tissue plasminogen activator (t-pa) which prolong its functional half-life
DE3911794A1 (de) * 1989-04-11 1990-10-18 Thomae Gmbh Dr K Monoklonale antikoerper gegen rekombinaten humanen gewebeplasminogenaktivator mit definierter epitopspezifitaet, deren herstellung und deren verwendung
DK182289D0 (da) * 1989-04-14 1989-04-14 Francesco Blasi Modifikation af proteiner
WO2014116706A1 (en) * 2013-01-22 2014-07-31 University Of Tennessee Research Foundation Tissue plasminogen activator antibodies and methods of use thereof
CN116068179B (zh) * 2022-07-20 2024-03-19 华中科技大学同济医学院附属同济医院 一种用于检测人组织型纤溶酶原激活物的胶体金试纸条

Family Cites Families (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
DE3222084A1 (de) * 1982-06-11 1983-12-15 Bayer Ag, 5090 Leverkusen Monoklonale antikoerper
JPS5951220A (ja) * 1982-08-02 1984-03-24 Asahi Chem Ind Co Ltd 新規なプラスミノ−ゲン・アクチベ−タ−およびその製法ならびにこれを含有する薬剤
DE3584902D1 (de) * 1984-02-29 1992-01-30 Asahi Chemical Ind Waessrige loesung eines darin in erhoehter konzentration aufgeloesten gewebe-plasminogen-aktivators und herstellungsverfahren.

Also Published As

Publication number Publication date
EP0190711A3 (en) 1986-11-05
ES551704A0 (es) 1987-11-16
ES8707565A1 (es) 1987-08-01
EP0190711B1 (en) 1989-04-26
ES557082A0 (es) 1987-08-01
CA1293459C (en) 1991-12-24
DE3663030D1 (en) 1989-06-01
EP0190711A2 (en) 1986-08-13

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Legal Events

Date Code Title Description
FD1A Patent lapsed

Effective date: 19990201