ES8800725A1 - Un procedimiento para producir un anticuerpo monoclonal - Google Patents
Un procedimiento para producir un anticuerpo monoclonalInfo
- Publication number
- ES8800725A1 ES8800725A1 ES551704A ES551704A ES8800725A1 ES 8800725 A1 ES8800725 A1 ES 8800725A1 ES 551704 A ES551704 A ES 551704A ES 551704 A ES551704 A ES 551704A ES 8800725 A1 ES8800725 A1 ES 8800725A1
- Authority
- ES
- Spain
- Prior art keywords
- plasminogen activator
- monoclonal antibodies
- tissue plasminogen
- human normal
- normal cells
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Expired
Links
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
- C12N9/14—Hydrolases (3)
- C12N9/48—Hydrolases (3) acting on peptide bonds (3.4)
- C12N9/50—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25)
- C12N9/64—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from animal tissue
- C12N9/6421—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from animal tissue from mammals
- C12N9/6424—Serine endopeptidases (3.4.21)
- C12N9/6456—Plasminogen activators
- C12N9/6459—Plasminogen activators t-plasminogen activator (3.4.21.68), i.e. tPA
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K16/00—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies
- C07K16/40—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies against enzymes
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Y—ENZYMES
- C12Y304/00—Hydrolases acting on peptide bonds, i.e. peptidases (3.4)
- C12Y304/21—Serine endopeptidases (3.4.21)
- C12Y304/21069—Protein C activated (3.4.21.69)
Landscapes
- Chemical & Material Sciences (AREA)
- Health & Medical Sciences (AREA)
- Organic Chemistry (AREA)
- Life Sciences & Earth Sciences (AREA)
- Genetics & Genomics (AREA)
- Engineering & Computer Science (AREA)
- Wood Science & Technology (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Biochemistry (AREA)
- Zoology (AREA)
- General Health & Medical Sciences (AREA)
- Molecular Biology (AREA)
- General Engineering & Computer Science (AREA)
- Biomedical Technology (AREA)
- Medicinal Chemistry (AREA)
- Biophysics (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Immunology (AREA)
- Biotechnology (AREA)
- Microbiology (AREA)
- Preparation Of Compounds By Using Micro-Organisms (AREA)
- Peptides Or Proteins (AREA)
Abstract
METODO DE PRODUCIR UN ANTICUERPO MONOCLONAL. CONSISTE EN: A) HABILITAR UNA CELULA HIBRIDA DE CELULAS DE MIELOMA DE RATONES Y ESPLENOCITOS DE RATONES INMUNIZADOS CON EL ACTIVADOR DE PLASMINOGENO DE TEJIDO, SIENDO LA CELULA HIBRIDA EL HIBRIDOMA X-21 QUE TIENE IFO 50086; B) CLONAR LA CELULA HIBRIDA PARA OBTENER CELULAS HIBRIDAS PRODUCTORAS DE ANTICUERPOS MONOCLONALES; C) CULTIVAR EN UN MEDIO DE CULTIVO LAS CELULAS HIBRIDAS PRODUCTORAS DE ANTICUERPOS MONOCLONALES; Y D) RECOGER UN ANTICUERPO MONOCLONAL SEPARANDOLO DE UN LIQUIDO SOBRENADANTE CONTENIDO EN EL MEDIO DE CULTIVO. TIENE UTILIDAD PARA PREPARAR COMPUESTOS FARMACEUTICOS.
Applications Claiming Priority (2)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
JP60020983A JP2610808B2 (ja) | 1985-02-07 | 1985-02-07 | 人正常細胞由来の組織型プラスミノーゲンアクチベーターに対するモノクロナル抗体を用いる免疫学的測定試薬 |
JP60021862A JPS61183299A (ja) | 1985-02-08 | 1985-02-08 | 人正常細胞由来の組織型プラスミノ−ゲンアクチベ−タ−に対する新規なモノクロナル抗体およびそれによる精製法ならびに検出法 |
Publications (2)
Publication Number | Publication Date |
---|---|
ES551704A0 ES551704A0 (es) | 1987-11-16 |
ES8800725A1 true ES8800725A1 (es) | 1987-11-16 |
Family
ID=26357991
Family Applications (2)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
ES551704A Expired ES8800725A1 (es) | 1985-02-07 | 1986-02-06 | Un procedimiento para producir un anticuerpo monoclonal |
ES557082A Expired ES8707565A1 (es) | 1985-02-07 | 1986-09-26 | Un procedimiento para producir un anticuerpo monoclonal |
Family Applications After (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
ES557082A Expired ES8707565A1 (es) | 1985-02-07 | 1986-09-26 | Un procedimiento para producir un anticuerpo monoclonal |
Country Status (4)
Country | Link |
---|---|
EP (1) | EP0190711B1 (es) |
CA (1) | CA1293459C (es) |
DE (1) | DE3663030D1 (es) |
ES (2) | ES8800725A1 (es) |
Families Citing this family (10)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
JPS61221128A (ja) * | 1985-03-26 | 1986-10-01 | Snow Brand Milk Prod Co Ltd | プラスミノ−ゲン活性化因子に対するモノクロ−ナル抗体とその調製方法及び該モノクロ−ナル抗体の使用方法 |
US5041381A (en) | 1986-07-03 | 1991-08-20 | Schering Corporation | Monoclonal antibodies against human interleukin-4 and hybridomas producing the same |
US4833085A (en) * | 1986-08-13 | 1989-05-23 | Monsanto Company | Monoclonal antibody specific for human colon fibroblast-derived t-PA |
FI89634C (fi) * | 1987-07-10 | 1993-10-25 | Yamanouchi Pharma Co Ltd | Foerfarande, anordning och kitt foer maetning av vaevnadsplasminogenaktivator-aktivitet |
US5411864A (en) * | 1987-11-05 | 1995-05-02 | Genentech, Inc. | Method of purifying recombinant proteins from corresponding host cell proteins |
US5225540A (en) * | 1988-04-26 | 1993-07-06 | Du Pont Merck Pharmaceutical Company | Monoclonal antibodies to tissue plasminogen activator (t-pa) which prolong its functional half-life |
DE3911794A1 (de) * | 1989-04-11 | 1990-10-18 | Thomae Gmbh Dr K | Monoklonale antikoerper gegen rekombinaten humanen gewebeplasminogenaktivator mit definierter epitopspezifitaet, deren herstellung und deren verwendung |
DK182289D0 (da) * | 1989-04-14 | 1989-04-14 | Francesco Blasi | Modifikation af proteiner |
WO2014116706A1 (en) * | 2013-01-22 | 2014-07-31 | University Of Tennessee Research Foundation | Tissue plasminogen activator antibodies and methods of use thereof |
CN116068179B (zh) * | 2022-07-20 | 2024-03-19 | 华中科技大学同济医学院附属同济医院 | 一种用于检测人组织型纤溶酶原激活物的胶体金试纸条 |
Family Cites Families (3)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
DE3222084A1 (de) * | 1982-06-11 | 1983-12-15 | Bayer Ag, 5090 Leverkusen | Monoklonale antikoerper |
JPS5951220A (ja) * | 1982-08-02 | 1984-03-24 | Asahi Chem Ind Co Ltd | 新規なプラスミノ−ゲン・アクチベ−タ−およびその製法ならびにこれを含有する薬剤 |
DE3584902D1 (de) * | 1984-02-29 | 1992-01-30 | Asahi Chemical Ind | Waessrige loesung eines darin in erhoehter konzentration aufgeloesten gewebe-plasminogen-aktivators und herstellungsverfahren. |
-
1986
- 1986-02-04 DE DE8686101395T patent/DE3663030D1/de not_active Expired
- 1986-02-04 EP EP86101395A patent/EP0190711B1/en not_active Expired
- 1986-02-05 CA CA000501129A patent/CA1293459C/en not_active Expired - Fee Related
- 1986-02-06 ES ES551704A patent/ES8800725A1/es not_active Expired
- 1986-09-26 ES ES557082A patent/ES8707565A1/es not_active Expired
Also Published As
Publication number | Publication date |
---|---|
EP0190711A3 (en) | 1986-11-05 |
ES551704A0 (es) | 1987-11-16 |
ES8707565A1 (es) | 1987-08-01 |
EP0190711B1 (en) | 1989-04-26 |
ES557082A0 (es) | 1987-08-01 |
CA1293459C (en) | 1991-12-24 |
DE3663030D1 (en) | 1989-06-01 |
EP0190711A2 (en) | 1986-08-13 |
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Legal Events
Date | Code | Title | Description |
---|---|---|---|
FD1A | Patent lapsed |
Effective date: 19990201 |