ES8602123A1 - Un metodo para clonar un gen que codifica la cobre-meta-lotioneina de levadura - Google Patents
Un metodo para clonar un gen que codifica la cobre-meta-lotioneina de levaduraInfo
- Publication number
- ES8602123A1 ES8602123A1 ES84534931A ES534931A ES8602123A1 ES 8602123 A1 ES8602123 A1 ES 8602123A1 ES 84534931 A ES84534931 A ES 84534931A ES 534931 A ES534931 A ES 534931A ES 8602123 A1 ES8602123 A1 ES 8602123A1
- Authority
- ES
- Spain
- Prior art keywords
- copper
- metallothionein gene
- yeast
- yeast copper
- metallothionein
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Expired
Links
Classifications
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
- C07K14/825—Metallothioneins
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
- C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
- C12N15/80—Vectors or expression systems specially adapted for eukaryotic hosts for fungi
- C12N15/81—Vectors or expression systems specially adapted for eukaryotic hosts for fungi for yeasts
Landscapes
- Life Sciences & Earth Sciences (AREA)
- Health & Medical Sciences (AREA)
- Genetics & Genomics (AREA)
- Chemical & Material Sciences (AREA)
- Organic Chemistry (AREA)
- Engineering & Computer Science (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Mycology (AREA)
- Biotechnology (AREA)
- General Engineering & Computer Science (AREA)
- Molecular Biology (AREA)
- Biophysics (AREA)
- Biomedical Technology (AREA)
- General Health & Medical Sciences (AREA)
- Wood Science & Technology (AREA)
- Zoology (AREA)
- Biochemistry (AREA)
- Physics & Mathematics (AREA)
- Microbiology (AREA)
- Plant Pathology (AREA)
- Gastroenterology & Hepatology (AREA)
- Medicinal Chemistry (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
Abstract
METODO PARA LA CLONACION DE TODA O UN PARTE DE LA UNIDAD DE EXPRESION DEL GEN DE COBRE-METALOTIONEINA DE LEVADURA. COMPRENDE TRATAR ADN CROMOSOMICO PROCEDENTE DE UNA CELULA DE LEVADURA, QUE PRODUCE COBRE-METALOTIONEINA, CON UNA O MAS ENDONUCLEASAS DE RESTRICCION; LIGAR EL FRAGMENTO DE ADN ESCINDIDO A UN VECTOR DE CLONACION DE ADN PARA INSERTARLE EL FRAGMENTO DE ADN ESCINDIDO; TRANSFORMAR UNA CELULA DE LEVADURA HOSPEDANTE CON EL VECTOR QUE TIENE INSERTADO EL CITADO FRAGMENTO DE ADN; Y SELECCIONAR LOS TRANSFORMANTES QUE LLEVAN TODA O PARTE DE LA UNIDAD DE EXPRESION DE DICHO GEN. LAS METALOTIONEINAS TIENEN APLICACIONES PARA QUELAR METALES DE TRANSICION EN CORRIENTES RESIDUALES INDUSTRIALES, Y PARA EL TRATRAMIENTO DE ENVENENAMIENTO POR METALES.
Applications Claiming Priority (2)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
US52066883A | 1983-08-08 | 1983-08-08 | |
US58465784A | 1984-03-02 | 1984-03-02 |
Publications (2)
Publication Number | Publication Date |
---|---|
ES534931A0 ES534931A0 (es) | 1985-11-01 |
ES8602123A1 true ES8602123A1 (es) | 1985-11-01 |
Family
ID=27060223
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
ES84534931A Expired ES8602123A1 (es) | 1983-08-08 | 1984-08-07 | Un metodo para clonar un gen que codifica la cobre-meta-lotioneina de levadura |
Country Status (6)
Country | Link |
---|---|
EP (1) | EP0134773A1 (es) |
AU (1) | AU3163784A (es) |
DK (1) | DK381684A (es) |
ES (1) | ES8602123A1 (es) |
GR (1) | GR80044B (es) |
PT (1) | PT79046B (es) |
Families Citing this family (6)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US4940661A (en) * | 1984-05-17 | 1990-07-10 | Genentech, Inc. | Metallothionein transcription control sequences and use thereof |
US4935350A (en) * | 1985-11-18 | 1990-06-19 | Amgen | Materials and methods for controlling plasmid copy number and stability |
AU614959B2 (en) * | 1987-05-07 | 1991-09-19 | Commonwealth Scientific And Industrial Research Organisation | Expression system |
TW282490B (es) * | 1992-12-15 | 1996-08-01 | Ciba Geigy Ag | |
EP0689433A4 (en) * | 1993-03-16 | 1999-10-20 | Univ Nebraska | NOVEL METAL BINDING AGENTS, METHODS AND COMPOSITIONS FOR USE IN TREATING METAL TOXICITY |
CN1062306C (zh) * | 1996-02-06 | 2001-02-21 | 北京大学 | 酵母菌Cu-金属硫蛋白及其生产方法 |
Family Cites Families (1)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
EP0078154A3 (en) * | 1981-10-28 | 1985-01-09 | The Regents Of The University Of California | Plasmids and cells for heterologous expression of metallothioneins and processes for producing and using the metallothionein |
-
1984
- 1984-08-06 AU AU31637/84A patent/AU3163784A/en not_active Abandoned
- 1984-08-07 EP EP84870112A patent/EP0134773A1/en not_active Withdrawn
- 1984-08-07 GR GR80044A patent/GR80044B/el unknown
- 1984-08-07 ES ES84534931A patent/ES8602123A1/es not_active Expired
- 1984-08-08 PT PT7904684A patent/PT79046B/pt unknown
- 1984-08-08 DK DK381684A patent/DK381684A/da not_active Application Discontinuation
Also Published As
Publication number | Publication date |
---|---|
DK381684D0 (da) | 1984-08-08 |
PT79046B (en) | 1986-11-13 |
EP0134773A1 (en) | 1985-03-20 |
ES534931A0 (es) | 1985-11-01 |
DK381684A (da) | 1985-02-09 |
AU3163784A (en) | 1985-02-14 |
PT79046A (en) | 1984-09-01 |
GR80044B (en) | 1984-12-10 |
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