ES8504927A1 - Un procedimiento para la separacion de los enzimas proteasa y amilasa extracelulares - Google Patents
Un procedimiento para la separacion de los enzimas proteasa y amilasa extracelularesInfo
- Publication number
- ES8504927A1 ES8504927A1 ES530897A ES530897A ES8504927A1 ES 8504927 A1 ES8504927 A1 ES 8504927A1 ES 530897 A ES530897 A ES 530897A ES 530897 A ES530897 A ES 530897A ES 8504927 A1 ES8504927 A1 ES 8504927A1
- Authority
- ES
- Spain
- Prior art keywords
- fermentation
- separation
- produced during
- amylase
- producing microorganisms
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Expired
Links
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
- C12N9/14—Hydrolases (3)
- C12N9/24—Hydrolases (3) acting on glycosyl compounds (3.2)
- C12N9/2402—Hydrolases (3) acting on glycosyl compounds (3.2) hydrolysing O- and S- glycosyl compounds (3.2.1)
- C12N9/2405—Glucanases
- C12N9/2408—Glucanases acting on alpha -1,4-glucosidic bonds
- C12N9/2411—Amylases
- C12N9/2414—Alpha-amylase (3.2.1.1.)
- C12N9/2417—Alpha-amylase (3.2.1.1.) from microbiological source
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
- C12N9/14—Hydrolases (3)
- C12N9/48—Hydrolases (3) acting on peptide bonds (3.4)
- C12N9/50—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25)
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
- C12N9/14—Hydrolases (3)
- C12N9/48—Hydrolases (3) acting on peptide bonds (3.4)
- C12N9/50—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25)
- C12N9/52—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from bacteria or Archaea
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
- C12N9/14—Hydrolases (3)
- C12N9/48—Hydrolases (3) acting on peptide bonds (3.4)
- C12N9/50—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25)
- C12N9/52—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from bacteria or Archaea
- C12N9/54—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from bacteria or Archaea bacteria being Bacillus
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
- Y10S—TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y10S435/00—Chemistry: molecular biology and microbiology
- Y10S435/814—Enzyme separation or purification
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
- Y10S—TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y10S435/00—Chemistry: molecular biology and microbiology
- Y10S435/814—Enzyme separation or purification
- Y10S435/816—Enzyme separation or purification by solubility
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
- Y10S—TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y10S435/00—Chemistry: molecular biology and microbiology
- Y10S435/8215—Microorganisms
- Y10S435/822—Microorganisms using bacteria or actinomycetales
- Y10S435/832—Bacillus
Landscapes
- Life Sciences & Earth Sciences (AREA)
- Health & Medical Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Engineering & Computer Science (AREA)
- Zoology (AREA)
- Organic Chemistry (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Genetics & Genomics (AREA)
- Wood Science & Technology (AREA)
- Molecular Biology (AREA)
- Biomedical Technology (AREA)
- Biotechnology (AREA)
- Microbiology (AREA)
- Biochemistry (AREA)
- General Engineering & Computer Science (AREA)
- General Health & Medical Sciences (AREA)
- Medicinal Chemistry (AREA)
- Enzymes And Modification Thereof (AREA)
- Preparation Of Compounds By Using Micro-Organisms (AREA)
- Detergent Compositions (AREA)
Abstract
PROCEDIMIENTO PARA LA SEPARACION DE LOS ENZIMAS PROTEASA Y AMILASA EXTRACELULARES.COMPRENDE: A) AGREGAR AL FILTRADO DE UN CALDO DE FERMENTACION DE UN MICROORGANISMO PRODUCTOR DE ENZIMAS (A) Y (B) UN POLIMERO (C) DE BAJO PESO MOLECULAR Y UN POLIMERO CATIONICO DE ALTO PESO MOLECULAR, SELECCIONADO ENTRE EL GRUPO FORMADO POR (D) Y (E) O UNA MEZCLA DE AMBOS; B) AGITAR LOS DOS POLIMEROS Y EL FILTRADO Y PRODUCIR LA SEPARACION DE FASES, ENTRE LA FASE QUE CONTIENE AL (C) RICA EN (A) CON RESPECTO A (B) Y UNA FASE QUE CONTIENE AL (D) O AL (E) RICA EN (B) CON RESPECTO A (A); C) SEPARAR LAS FASES FORMADAS EN LA ETAPA (C); D) RECUPERAR LOS ENZIMAS DE LA FASE QUE CONTIENE (C), PARA OBTENER UN PRODUCTO DE (A)/(B) RICO EN (A); Y E) RECUPERAR LOS ENZIMAS DE LA FASE QUE CONTIENE AL (D) O AL (E), PARA OBTENER UN PRODUCTO DE (A)/(B) RICO EN (B); SIENDO: (A) PROTEASA; (B) AMILASA; (C) POLIETILENGLICOL; (D) COPOLIMEROS DE EPIHALOHIDRINA/POLIAMINA Y (E) COPOLIMEROS DE DEXTRANO.
Applications Claiming Priority (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
US06/480,245 US4508825A (en) | 1983-03-30 | 1983-03-30 | Method for the separation of extracellular amylase and protease produced during the fermentation of enzyme producing microorganisms |
Publications (2)
Publication Number | Publication Date |
---|---|
ES8504927A1 true ES8504927A1 (es) | 1985-05-01 |
ES530897A0 ES530897A0 (es) | 1985-05-01 |
Family
ID=23907225
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
ES530897A Granted ES530897A0 (es) | 1983-03-30 | 1984-03-23 | Un procedimiento para la separacion de los enzimas proteasa y amilasa extracelulares |
Country Status (6)
Country | Link |
---|---|
US (1) | US4508825A (es) |
EP (1) | EP0121185B1 (es) |
DE (1) | DE3479038D1 (es) |
DK (1) | DK120284A (es) |
ES (1) | ES530897A0 (es) |
MX (1) | MX7640E (es) |
Families Citing this family (18)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
FR2545501B1 (fr) * | 1983-05-06 | 1985-08-02 | Elf Bio Rech | Procede de purification de la dextrane-saccharase |
US4690892A (en) * | 1985-04-29 | 1987-09-01 | Union Carbide Corporation | Process for the recycle of phase-forming liquids of multi-phase aqueous systems |
US4728613A (en) * | 1985-09-04 | 1988-03-01 | Miles Laboratories, Inc. | Method for the recovery of extracellular enzymes from whole fermentation beer |
EP0262651A3 (en) * | 1986-09-30 | 1990-06-20 | Union Carbide Corporation | Isolation of enzyme values from solution |
US5139943A (en) * | 1989-06-13 | 1992-08-18 | Genencor International, Inc. | Processes for the recovery of microbially produced chymosin |
US5151358A (en) * | 1989-06-13 | 1992-09-29 | Genencor International, Inc. | Processes for the recovery of naturally produced chymosin |
US4997759A (en) * | 1990-02-05 | 1991-03-05 | American Cyanamid Company | Use of blends of mannich acrylamide polymers and dimethyldiallylammonium halide polymers for flocculating enzyme broth streams |
SG45199A1 (en) * | 1990-04-05 | 1998-01-16 | Kao Corp | Detergent composition |
US5290474A (en) * | 1990-10-05 | 1994-03-01 | Genencor International, Inc. | Detergent composition for treating cotton-containing fabrics containing a surfactant and a cellulase composition containing endolucanase III from trichoderma ssp |
US5328841A (en) * | 1990-10-05 | 1994-07-12 | Genencor International, Inc. | Methods for isolating EG III cellulase component and EG III cellulase in polyethylene glycol using inorganic salt and polyethylene glycol |
CA2093422C (en) * | 1990-10-05 | 2001-04-03 | DETERGENT COMPOSITIONS CONTAINING LOW CBH I CONTENT CELLULASE COMPOSITIONS | |
EP0581002A2 (en) * | 1992-07-30 | 1994-02-02 | Cytec Technology Corp. | Use of blends of mannich acrylamide polymers and quaternary polyamines for flocculating enzyme broth streams |
JP3560653B2 (ja) * | 1993-09-30 | 2004-09-02 | ヒゲタ醤油株式会社 | イツリンaの製造法及び抗深在性真菌症剤 |
JPH10500573A (ja) * | 1994-05-23 | 1998-01-20 | イー・アイ・デユポン・ドウ・ヌムール・アンド・カンパニー | 複合マトリックスからの汚染微生物の迅速な分離および同定方法 |
MY127310A (en) * | 2000-04-28 | 2006-11-30 | Sumitomo Rubber Ind | Deproteinizing agent, deproteinized natural rubber latex using the same, and method of producing rubber product |
WO2006003134A1 (en) * | 2004-06-29 | 2006-01-12 | Ares Trading S.A. | Process for the purification of il-18 binding protein |
US20070148302A1 (en) * | 2005-12-27 | 2007-06-28 | Ricardo Villota | Nutritionally enhanced pasta |
US8268975B2 (en) | 2009-04-03 | 2012-09-18 | Dow Agrosciences Llc | Demulsification compositions, systems and methods for demulsifying and separating aqueous emulsions |
Family Cites Families (4)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US2810677A (en) * | 1955-06-24 | 1957-10-22 | Corn Prod Refining Co | Recovery and purification of enzymes |
DE2512735A1 (de) * | 1975-03-22 | 1976-09-30 | Henkel & Cie Gmbh | Verfahren zur gewinnung von proteinen aus waessrigen protein- loesungen |
DE2639129C3 (de) * | 1976-08-31 | 1979-10-04 | Gesellschaft Fuer Biotechnologische Forschung Mbh (Gbf), 3300 Braunschweig | Verfahren zur Abtrennung von Enzymen |
GB1589581A (en) * | 1976-04-14 | 1981-05-13 | Biotechnolog Forschung Gmbh | Process for the separation of enzymes |
-
1983
- 1983-03-30 US US06/480,245 patent/US4508825A/en not_active Expired - Lifetime
-
1984
- 1984-02-28 DK DK120284A patent/DK120284A/da not_active Application Discontinuation
- 1984-02-28 MX MX84940U patent/MX7640E/es unknown
- 1984-03-22 EP EP84103170A patent/EP0121185B1/en not_active Expired
- 1984-03-22 DE DE8484103170T patent/DE3479038D1/de not_active Expired
- 1984-03-23 ES ES530897A patent/ES530897A0/es active Granted
Also Published As
Publication number | Publication date |
---|---|
EP0121185A3 (en) | 1987-02-25 |
DE3479038D1 (en) | 1989-08-24 |
DK120284D0 (da) | 1984-02-28 |
DK120284A (da) | 1984-10-01 |
US4508825A (en) | 1985-04-02 |
EP0121185A2 (en) | 1984-10-10 |
MX7640E (es) | 1990-05-16 |
ES530897A0 (es) | 1985-05-01 |
EP0121185B1 (en) | 1989-07-19 |
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Legal Events
Date | Code | Title | Description |
---|---|---|---|
PC1A | Transfer granted | ||
FD1A | Patent lapsed |
Effective date: 20040216 |