ES8502261A1 - Un metodo para determinar ligandos en una muestra. - Google Patents

Un metodo para determinar ligandos en una muestra.

Info

Publication number
ES8502261A1
ES8502261A1 ES527085A ES527085A ES8502261A1 ES 8502261 A1 ES8502261 A1 ES 8502261A1 ES 527085 A ES527085 A ES 527085A ES 527085 A ES527085 A ES 527085A ES 8502261 A1 ES8502261 A1 ES 8502261A1
Authority
ES
Spain
Prior art keywords
reagents
disclosure relates
carboxyfluoresceins
substituted
fluid
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired
Application number
ES527085A
Other languages
English (en)
Other versions
ES527085A0 (es
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Abbott Laboratories
Original Assignee
Abbott Laboratories
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Abbott Laboratories filed Critical Abbott Laboratories
Publication of ES527085A0 publication Critical patent/ES527085A0/es
Publication of ES8502261A1 publication Critical patent/ES8502261A1/es
Expired legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07D—HETEROCYCLIC COMPOUNDS
    • C07D407/00—Heterocyclic compounds containing two or more hetero rings, at least one ring having oxygen atoms as the only ring hetero atoms, not provided for by group C07D405/00
    • C07D407/02—Heterocyclic compounds containing two or more hetero rings, at least one ring having oxygen atoms as the only ring hetero atoms, not provided for by group C07D405/00 containing two hetero rings
    • C07D407/12—Heterocyclic compounds containing two or more hetero rings, at least one ring having oxygen atoms as the only ring hetero atoms, not provided for by group C07D405/00 containing two hetero rings linked by a chain containing hetero atoms as chain links
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07D—HETEROCYCLIC COMPOUNDS
    • C07D311/00—Heterocyclic compounds containing six-membered rings having one oxygen atom as the only hetero atom, condensed with other rings
    • C07D311/02—Heterocyclic compounds containing six-membered rings having one oxygen atom as the only hetero atom, condensed with other rings ortho- or peri-condensed with carbocyclic rings or ring systems
    • C07D311/78—Ring systems having three or more relevant rings
    • C07D311/80—Dibenzopyrans; Hydrogenated dibenzopyrans
    • C07D311/82—Xanthenes
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/531—Production of immunochemical test materials
    • G01N33/532—Production of labelled immunochemicals
    • G01N33/533—Production of labelled immunochemicals with fluorescent label

Landscapes

  • Chemical & Material Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Organic Chemistry (AREA)
  • Immunology (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Urology & Nephrology (AREA)
  • Biomedical Technology (AREA)
  • Hematology (AREA)
  • Molecular Biology (AREA)
  • Food Science & Technology (AREA)
  • General Health & Medical Sciences (AREA)
  • Cell Biology (AREA)
  • Medicinal Chemistry (AREA)
  • Physics & Mathematics (AREA)
  • Analytical Chemistry (AREA)
  • Biochemistry (AREA)
  • Microbiology (AREA)
  • General Physics & Mathematics (AREA)
  • Pathology (AREA)
  • Biotechnology (AREA)
  • Investigating Or Analysing Biological Materials (AREA)
  • Pyrane Compounds (AREA)
  • Investigating Or Analysing Materials By The Use Of Chemical Reactions (AREA)
  • Organic Low-Molecular-Weight Compounds And Preparation Thereof (AREA)

Abstract

METODO PARA DETERMINAR LIGANDOS EN UNA MUESTRA DE FLUIDOS BIOLOGICOS TALES COMO SUERO, PLASMA, FLUIDO ESPIRAL, FLUIDO AMNIOTICO Y ORINA.CONSISTE EN SOMETER A UNA REACCION COMPETITIVA LA MUESTRA A ANALIZAR, CON UN TRAZADOR DE FORMULA GENERAL (I) O (II) O CON SUS SALES FISIOLOGICAMENTE ACEPTABLES Y CON UN ANTICUERPO CAPAZ DE RECONOCER ESPECIFICAMENTE DICHO LIGANDO Y DICHO TRAZADOR, PARA DAR LUGAR A LA FORMACION DE LOS CORRESPONDIENTES COMPLEJOS LIGANDO-ANTICUERPO Y TRAZADOR-ANTICUERPO. A CONTINUACION SE EXCITA LA MEZCLA DE REACCION CON LUZ POLARIZADA Y SE MIDE LA POLARIZACION DE LA FLUORESCENCIA EMITIDA, DETERMINANDO CON ELLO LA CONCENTRACION DE LIGANDO PRESENTE EN LA MUESTRA.
ES527085A 1982-11-08 1983-11-07 Un metodo para determinar ligandos en una muestra. Expired ES8502261A1 (es)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
US44006982A 1982-11-08 1982-11-08

Publications (2)

Publication Number Publication Date
ES527085A0 ES527085A0 (es) 1984-12-16
ES8502261A1 true ES8502261A1 (es) 1984-12-16

Family

ID=23747296

Family Applications (1)

Application Number Title Priority Date Filing Date
ES527085A Expired ES8502261A1 (es) 1982-11-08 1983-11-07 Un metodo para determinar ligandos en una muestra.

Country Status (4)

Country Link
EP (1) EP0108403A3 (es)
JP (1) JPS59101481A (es)
CA (1) CA1195995A (es)
ES (1) ES8502261A1 (es)

Families Citing this family (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4681859A (en) * 1984-09-21 1987-07-21 Ortho Diagnostic Systems Inc. Fluorescence polarization immunoassay for heavy antigens
IL76447A0 (en) * 1984-10-25 1986-01-31 Abbott Lab Fluorescence polarization assay,reagents,and method of making reagents,for determination of digitoxin
US4698315A (en) * 1985-04-22 1987-10-06 Hoffmann-La Roche Inc. Method and kit for determining total digoxin levels involving agent to dissociate digoxin-protein complex
EP0226730B1 (en) * 1985-10-15 1994-03-02 Abbott Laboratories Compounds and assay for tricyclic antidepressants
DE3628795A1 (de) * 1986-08-25 1988-03-03 Hoechst Ag Neue thyroninderivate
US5352803A (en) * 1992-03-30 1994-10-04 Abbott Laboratories 5(6)-methyl substituted fluorescein derivatives

Family Cites Families (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CA1121345A (en) * 1979-03-05 1982-04-06 Robert A. Yoshida Method for competitive protein binding assays inhibiting non-specific interference
US4329461A (en) * 1980-01-11 1982-05-11 Syva Company Fluorescent thyroid hormone conjugates and their uses
EP0047459B1 (de) * 1980-09-08 1984-11-21 International Diagnostic Technology, Inc. Fluoreszierendes Reagenz und Immunofluoreszenz-Bestimmungsmethode
AU555213B2 (en) * 1981-02-17 1986-09-18 Abbott Laboratories N-substituted-amido-fluoresein derivatives
NZ199629A (en) * 1981-02-17 1984-11-09 Abbott Lab Fluorescent polarisation immunoassay and certain substituted carboxy fluoresceins

Also Published As

Publication number Publication date
EP0108403A2 (en) 1984-05-16
JPS59101481A (ja) 1984-06-12
CA1195995A (en) 1985-10-29
EP0108403A3 (en) 1984-07-04
ES527085A0 (es) 1984-12-16

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Legal Events

Date Code Title Description
FD1A Patent lapsed

Effective date: 19960603