ES8502261A1 - Un metodo para determinar ligandos en una muestra. - Google Patents
Un metodo para determinar ligandos en una muestra.Info
- Publication number
- ES8502261A1 ES8502261A1 ES527085A ES527085A ES8502261A1 ES 8502261 A1 ES8502261 A1 ES 8502261A1 ES 527085 A ES527085 A ES 527085A ES 527085 A ES527085 A ES 527085A ES 8502261 A1 ES8502261 A1 ES 8502261A1
- Authority
- ES
- Spain
- Prior art keywords
- reagents
- disclosure relates
- carboxyfluoresceins
- substituted
- fluid
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Expired
Links
Classifications
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07D—HETEROCYCLIC COMPOUNDS
- C07D407/00—Heterocyclic compounds containing two or more hetero rings, at least one ring having oxygen atoms as the only ring hetero atoms, not provided for by group C07D405/00
- C07D407/02—Heterocyclic compounds containing two or more hetero rings, at least one ring having oxygen atoms as the only ring hetero atoms, not provided for by group C07D405/00 containing two hetero rings
- C07D407/12—Heterocyclic compounds containing two or more hetero rings, at least one ring having oxygen atoms as the only ring hetero atoms, not provided for by group C07D405/00 containing two hetero rings linked by a chain containing hetero atoms as chain links
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07D—HETEROCYCLIC COMPOUNDS
- C07D311/00—Heterocyclic compounds containing six-membered rings having one oxygen atom as the only hetero atom, condensed with other rings
- C07D311/02—Heterocyclic compounds containing six-membered rings having one oxygen atom as the only hetero atom, condensed with other rings ortho- or peri-condensed with carbocyclic rings or ring systems
- C07D311/78—Ring systems having three or more relevant rings
- C07D311/80—Dibenzopyrans; Hydrogenated dibenzopyrans
- C07D311/82—Xanthenes
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
- G01N33/531—Production of immunochemical test materials
- G01N33/532—Production of labelled immunochemicals
- G01N33/533—Production of labelled immunochemicals with fluorescent label
Landscapes
- Chemical & Material Sciences (AREA)
- Health & Medical Sciences (AREA)
- Organic Chemistry (AREA)
- Immunology (AREA)
- Life Sciences & Earth Sciences (AREA)
- Engineering & Computer Science (AREA)
- Urology & Nephrology (AREA)
- Biomedical Technology (AREA)
- Hematology (AREA)
- Molecular Biology (AREA)
- Food Science & Technology (AREA)
- General Health & Medical Sciences (AREA)
- Cell Biology (AREA)
- Medicinal Chemistry (AREA)
- Physics & Mathematics (AREA)
- Analytical Chemistry (AREA)
- Biochemistry (AREA)
- Microbiology (AREA)
- General Physics & Mathematics (AREA)
- Pathology (AREA)
- Biotechnology (AREA)
- Investigating Or Analysing Biological Materials (AREA)
- Pyrane Compounds (AREA)
- Investigating Or Analysing Materials By The Use Of Chemical Reactions (AREA)
- Organic Low-Molecular-Weight Compounds And Preparation Thereof (AREA)
Abstract
METODO PARA DETERMINAR LIGANDOS EN UNA MUESTRA DE FLUIDOS BIOLOGICOS TALES COMO SUERO, PLASMA, FLUIDO ESPIRAL, FLUIDO AMNIOTICO Y ORINA.CONSISTE EN SOMETER A UNA REACCION COMPETITIVA LA MUESTRA A ANALIZAR, CON UN TRAZADOR DE FORMULA GENERAL (I) O (II) O CON SUS SALES FISIOLOGICAMENTE ACEPTABLES Y CON UN ANTICUERPO CAPAZ DE RECONOCER ESPECIFICAMENTE DICHO LIGANDO Y DICHO TRAZADOR, PARA DAR LUGAR A LA FORMACION DE LOS CORRESPONDIENTES COMPLEJOS LIGANDO-ANTICUERPO Y TRAZADOR-ANTICUERPO. A CONTINUACION SE EXCITA LA MEZCLA DE REACCION CON LUZ POLARIZADA Y SE MIDE LA POLARIZACION DE LA FLUORESCENCIA EMITIDA, DETERMINANDO CON ELLO LA CONCENTRACION DE LIGANDO PRESENTE EN LA MUESTRA.
Applications Claiming Priority (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
US44006982A | 1982-11-08 | 1982-11-08 |
Publications (2)
Publication Number | Publication Date |
---|---|
ES527085A0 ES527085A0 (es) | 1984-12-16 |
ES8502261A1 true ES8502261A1 (es) | 1984-12-16 |
Family
ID=23747296
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
ES527085A Expired ES8502261A1 (es) | 1982-11-08 | 1983-11-07 | Un metodo para determinar ligandos en una muestra. |
Country Status (4)
Country | Link |
---|---|
EP (1) | EP0108403A3 (es) |
JP (1) | JPS59101481A (es) |
CA (1) | CA1195995A (es) |
ES (1) | ES8502261A1 (es) |
Families Citing this family (6)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US4681859A (en) * | 1984-09-21 | 1987-07-21 | Ortho Diagnostic Systems Inc. | Fluorescence polarization immunoassay for heavy antigens |
IL76447A0 (en) * | 1984-10-25 | 1986-01-31 | Abbott Lab | Fluorescence polarization assay,reagents,and method of making reagents,for determination of digitoxin |
US4698315A (en) * | 1985-04-22 | 1987-10-06 | Hoffmann-La Roche Inc. | Method and kit for determining total digoxin levels involving agent to dissociate digoxin-protein complex |
EP0226730B1 (en) * | 1985-10-15 | 1994-03-02 | Abbott Laboratories | Compounds and assay for tricyclic antidepressants |
DE3628795A1 (de) * | 1986-08-25 | 1988-03-03 | Hoechst Ag | Neue thyroninderivate |
US5352803A (en) * | 1992-03-30 | 1994-10-04 | Abbott Laboratories | 5(6)-methyl substituted fluorescein derivatives |
Family Cites Families (5)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CA1121345A (en) * | 1979-03-05 | 1982-04-06 | Robert A. Yoshida | Method for competitive protein binding assays inhibiting non-specific interference |
US4329461A (en) * | 1980-01-11 | 1982-05-11 | Syva Company | Fluorescent thyroid hormone conjugates and their uses |
EP0047459B1 (de) * | 1980-09-08 | 1984-11-21 | International Diagnostic Technology, Inc. | Fluoreszierendes Reagenz und Immunofluoreszenz-Bestimmungsmethode |
AU555213B2 (en) * | 1981-02-17 | 1986-09-18 | Abbott Laboratories | N-substituted-amido-fluoresein derivatives |
NZ199629A (en) * | 1981-02-17 | 1984-11-09 | Abbott Lab | Fluorescent polarisation immunoassay and certain substituted carboxy fluoresceins |
-
1983
- 1983-11-02 CA CA000440276A patent/CA1195995A/en not_active Expired
- 1983-11-04 EP EP83111010A patent/EP0108403A3/en not_active Withdrawn
- 1983-11-07 ES ES527085A patent/ES8502261A1/es not_active Expired
- 1983-11-08 JP JP20844183A patent/JPS59101481A/ja active Pending
Also Published As
Publication number | Publication date |
---|---|
EP0108403A2 (en) | 1984-05-16 |
JPS59101481A (ja) | 1984-06-12 |
CA1195995A (en) | 1985-10-29 |
EP0108403A3 (en) | 1984-07-04 |
ES527085A0 (es) | 1984-12-16 |
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Legal Events
Date | Code | Title | Description |
---|---|---|---|
FD1A | Patent lapsed |
Effective date: 19960603 |