ES8401136A1 - Un metodo enzimatico mejorado para el analisis cinetico de fluidos biologicos. - Google Patents

Un metodo enzimatico mejorado para el analisis cinetico de fluidos biologicos.

Info

Publication number
ES8401136A1
ES8401136A1 ES515307A ES515307A ES8401136A1 ES 8401136 A1 ES8401136 A1 ES 8401136A1 ES 515307 A ES515307 A ES 515307A ES 515307 A ES515307 A ES 515307A ES 8401136 A1 ES8401136 A1 ES 8401136A1
Authority
ES
Spain
Prior art keywords
sensitivity
increasing
assays
improved
cyclodextrin
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired
Application number
ES515307A
Other languages
English (en)
Other versions
ES515307A0 (es
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
American Hospital Supply Corp
Original Assignee
American Hospital Supply Corp
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by American Hospital Supply Corp filed Critical American Hospital Supply Corp
Publication of ES515307A0 publication Critical patent/ES515307A0/es
Publication of ES8401136A1 publication Critical patent/ES8401136A1/es
Expired legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/34—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving hydrolase
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/34—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving hydrolase
    • C12Q1/40—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving hydrolase involving amylase
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/52—Use of compounds or compositions for colorimetric, spectrophotometric or fluorometric investigation, e.g. use of reagent paper and including single- and multilayer analytical elements
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2334/00—O-linked chromogens for determinations of hydrolase enzymes, e.g. glycosidases, phosphatases, esterases
    • C12Q2334/10—O-linked chromogens for determinations of hydrolase enzymes, e.g. glycosidases, phosphatases, esterases p-Nitrophenol derivatives
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N2333/00—Assays involving biological materials from specific organisms or of a specific nature
    • G01N2333/90—Enzymes; Proenzymes
    • G01N2333/914—Hydrolases (3)
    • G01N2333/924—Hydrolases (3) acting on glycosyl compounds (3.2)
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N2400/00—Assays, e.g. immunoassays or enzyme assays, involving carbohydrates
    • G01N2400/10—Polysaccharides, i.e. having more than five saccharide radicals attached to each other by glycosidic linkages; Derivatives thereof, e.g. ethers, esters
    • G01N2400/12—Homoglycans, i.e. polysaccharides having a main chain consisting of one single sugar
    • G01N2400/14—Homoglycans, i.e. polysaccharides having a main chain consisting of one single sugar alpha-D-Glucans, i.e. having alpha 1,n (n=3,4,6) linkages between saccharide units, e.g. pullulan
    • G01N2400/18—Cyclodextrin
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
    • Y10T—TECHNICAL SUBJECTS COVERED BY FORMER US CLASSIFICATION
    • Y10T436/00—Chemistry: analytical and immunological testing
    • Y10T436/14—Heterocyclic carbon compound [i.e., O, S, N, Se, Te, as only ring hetero atom]
    • Y10T436/142222—Hetero-O [e.g., ascorbic acid, etc.]
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
    • Y10T—TECHNICAL SUBJECTS COVERED BY FORMER US CLASSIFICATION
    • Y10T436/00—Chemistry: analytical and immunological testing
    • Y10T436/14—Heterocyclic carbon compound [i.e., O, S, N, Se, Te, as only ring hetero atom]
    • Y10T436/142222—Hetero-O [e.g., ascorbic acid, etc.]
    • Y10T436/143333—Saccharide [e.g., DNA, etc.]
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
    • Y10T—TECHNICAL SUBJECTS COVERED BY FORMER US CLASSIFICATION
    • Y10T436/00—Chemistry: analytical and immunological testing
    • Y10T436/14—Heterocyclic carbon compound [i.e., O, S, N, Se, Te, as only ring hetero atom]
    • Y10T436/142222—Hetero-O [e.g., ascorbic acid, etc.]
    • Y10T436/143333—Saccharide [e.g., DNA, etc.]
    • Y10T436/144444—Glucose

Landscapes

  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Organic Chemistry (AREA)
  • Immunology (AREA)
  • Zoology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Molecular Biology (AREA)
  • Wood Science & Technology (AREA)
  • Biotechnology (AREA)
  • Hematology (AREA)
  • Microbiology (AREA)
  • Analytical Chemistry (AREA)
  • Physics & Mathematics (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • General Engineering & Computer Science (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Genetics & Genomics (AREA)
  • Biophysics (AREA)
  • Biomedical Technology (AREA)
  • Urology & Nephrology (AREA)
  • Cell Biology (AREA)
  • Food Science & Technology (AREA)
  • Medicinal Chemistry (AREA)
  • General Physics & Mathematics (AREA)
  • Pathology (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)

Abstract

METODO ENZIMATICO PARA EL ANALISIS CINETICO DE FLUIDOS BIOLOGICOS.COMPRENDE LAS SIGUIENTES OPERACIONES: PRIMERA, SE EFECTUA LA HIDROLISIS DE UN INDICADOR DE NITROFENOL A PARTIR DE SU SUSTRATO, A UN PH NEUTRO O APROXIMADAMENTE NEUTRO; SEGUNDA, SE AÑADE AL FLUIDO BIOLOGICO UNA CANTIDAD EFECTIVA DE SENSIBILIZACION DE CICLODEXTRINA AL OBJETO DE EFECTUAR UN DESPLAZAMIENTO HIPOCROMICO EN LA LONGITUD DE ONDA DEL HOMOLOGO NO IONIZADO DEL COMPUESTO INDICADOR, AUMENTANDO ASI LA PROPORCION DE INDICADOR HOMOLOGO IONIZADO EN DICHO FLUIDO BIOLOGICO; Y POR ULTIMO, SE MIDE EL NIVEL DE INDICADOR IONIZADOEN EL FLUIDO BIOLOGICO A LOS MAXIMOS DE ABSORBENCIA DE DICHO INDICADOR IONICO.
ES515307A 1981-08-28 1982-08-27 Un metodo enzimatico mejorado para el analisis cinetico de fluidos biologicos. Expired ES8401136A1 (es)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
US06/297,483 US4451563A (en) 1981-08-28 1981-08-28 Method for increasing the sensitivity of assays

Publications (2)

Publication Number Publication Date
ES515307A0 ES515307A0 (es) 1983-11-16
ES8401136A1 true ES8401136A1 (es) 1983-11-16

Family

ID=23146505

Family Applications (1)

Application Number Title Priority Date Filing Date
ES515307A Expired ES8401136A1 (es) 1981-08-28 1982-08-27 Un metodo enzimatico mejorado para el analisis cinetico de fluidos biologicos.

Country Status (7)

Country Link
US (1) US4451563A (es)
EP (1) EP0086821B1 (es)
JP (1) JPS58501357A (es)
CA (1) CA1201368A (es)
DE (1) DE3279569D1 (es)
ES (1) ES8401136A1 (es)
WO (1) WO1983000876A1 (es)

Families Citing this family (21)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4511651A (en) * 1982-07-30 1985-04-16 American Monitor Corporation Reagent composition and assay for the determination of γ-glutamyltransferase activity
JPS59219270A (ja) * 1983-05-30 1984-12-10 Wako Pure Chem Ind Ltd テトラゾリウム塩を含有する溶液の安定化方法
DE3328616A1 (de) * 1983-08-08 1985-02-28 Boehringer Mannheim Gmbh, 6800 Mannheim Oligoglucosidderivate
EP0134291B1 (de) * 1983-09-07 1986-08-13 American Hospital Supply Corporation Rekonstituierbares Trockenreagenz für diagnostische Zwecke und Verfahren zu seiner Herstellung
JPS61112092A (ja) * 1984-11-07 1986-05-30 Meiji Seika Kaisha Ltd 新規グルコサミン誘導体及びこれを基質として用いるＮ−アセチル−β−Ｄ−グルコサミニダ−ゼの活性測定試薬
US5011964A (en) * 1985-03-07 1991-04-30 Cornell Research Foundation, Inc. Novel diacylglycerophosphoric acid esters and use thereof as substrates in enzyme assays
GB8505899D0 (en) * 1985-03-07 1985-04-11 Boots Celltech Diagnostics Assay reagents
US4963479A (en) * 1986-10-07 1990-10-16 Hoechst Celanese Corporation Reagent system for an alpha-amylase assay containing aromatic substituted glycoside
US5158872A (en) * 1986-10-07 1992-10-27 Hoechst Celanese Corporation Aromatic substituted glycoside
JP2741031B2 (ja) * 1987-05-12 1998-04-15 株式会社シノテスト アミラーゼ測定方法
JP2681635B2 (ja) * 1987-05-13 1997-11-26 オリエンタル酵母工業株式会社 分析感度及び分析精度を向上させる方法
DE3743908A1 (de) * 1987-12-23 1989-07-06 Boehringer Mannheim Gmbh Neue oligoglucoside
US5032503A (en) * 1988-06-22 1991-07-16 Microgenics Corporation Liquid single reagent for air enzyme complementation assay
EP0407574A1 (en) * 1988-11-10 1991-01-16 Oriental Yeast Co., Ltd. Method for improving sensitivity and accuracy of analysis
US5086001A (en) * 1989-12-01 1992-02-04 Baxter International, Inc. Automated test method for evaluating the physical compatibility of intravenous drugs in solutions
JPH0484920U (es) * 1990-11-30 1992-07-23
US5874229A (en) * 1995-08-07 1999-02-23 Kyoto Daiichi Kagaku Co., Ltd. Method for avoiding influence of hemoglobin
GB9727355D0 (en) * 1997-12-24 1998-02-25 Kalibrant Limited A composition for use in fluorescence assay systems
DE10032290C2 (de) * 2000-07-03 2002-05-08 Roche Diagnostics Gmbh Adsorptionsmittelhaltige Kontrollflüssigkeit
JP5614281B2 (ja) * 2008-04-14 2014-10-29 和光純薬工業株式会社 フェニルホスホリルコリン誘導体
ITMI20121636A1 (it) * 2012-10-01 2014-04-02 Andrea Mozzarelli Metodo per la determinazione dell'attivita' di alfa-amilasi

Family Cites Families (12)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4121975A (en) * 1976-08-20 1978-10-24 Syva Company Pretreatment of samples for polyiodothyronine assays
US4102747A (en) * 1977-07-28 1978-07-25 American Hospital Supply Corporation Amylase determination
JPS5443791A (en) * 1977-09-13 1979-04-06 Seishin Seiyaku Kk Method and reagent for measuring hydrogen peroxide
US4233403A (en) * 1978-02-27 1980-11-11 E. I. Du Pont De Nemours And Company Amylase assay
US4225672A (en) * 1979-02-27 1980-09-30 Hall Leo M Method for producing maltooligosaccharide glycosides
US4294923A (en) * 1979-04-23 1981-10-13 Smith Robert E Substrates and method for determining enzymes
US4252783A (en) * 1979-06-18 1981-02-24 Syva Company Reducing fluorescent background in fluorescent immunoassays
GB2064527B (en) * 1979-12-08 1984-05-02 Nippon Shinyaku Co Ltd Moranoline derivatives and process for preparation thereof
DE3000292A1 (de) * 1980-01-05 1981-07-09 Behringwerke Ag, 3550 Marburg Indoxylmaltodextrine, verfahren zu ihrer herstellung und ihre verwendung
JPS5768798A (en) * 1980-10-14 1982-04-27 Toyo Jozo Co Ltd Novel measurement of amylase activity
JPS5774099A (en) * 1980-10-28 1982-05-10 Kanto Kagaku Kk Reagent for measuring activity of gamma-cultanyl transpeptidase
JPS57102198A (en) * 1980-12-15 1982-06-25 Toyobo Co Ltd Measuring method of amylase activity

Also Published As

Publication number Publication date
JPS58501357A (ja) 1983-08-18
DE3279569D1 (en) 1989-04-27
EP0086821B1 (en) 1989-03-22
ES515307A0 (es) 1983-11-16
CA1201368A (en) 1986-03-04
EP0086821A4 (en) 1984-06-05
WO1983000876A1 (en) 1983-03-17
US4451563A (en) 1984-05-29
JPH0229320B2 (es) 1990-06-28
EP0086821A1 (en) 1983-08-31

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