ES467667A1 - Un metodo para la determinacion de peroxidasa en una muestrafisiologica - Google Patents
Un metodo para la determinacion de peroxidasa en una muestrafisiologicaInfo
- Publication number
- ES467667A1 ES467667A1 ES467667A ES467667A ES467667A1 ES 467667 A1 ES467667 A1 ES 467667A1 ES 467667 A ES467667 A ES 467667A ES 467667 A ES467667 A ES 467667A ES 467667 A1 ES467667 A1 ES 467667A1
- Authority
- ES
- Spain
- Prior art keywords
- peroxidase
- mixt
- esp
- pref
- degrees
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Expired
Links
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/26—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving oxidoreductase
- C12Q1/28—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving oxidoreductase involving peroxidase
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q2326/00—Chromogens for determinations of oxidoreductase enzymes
- C12Q2326/50—Phenols; Naphthols; Catechols
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q2326/00—Chromogens for determinations of oxidoreductase enzymes
- C12Q2326/90—Developer
- C12Q2326/96—4-Amino-antipyrine
Landscapes
- Chemical & Material Sciences (AREA)
- Organic Chemistry (AREA)
- Life Sciences & Earth Sciences (AREA)
- Zoology (AREA)
- Wood Science & Technology (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Health & Medical Sciences (AREA)
- Engineering & Computer Science (AREA)
- Microbiology (AREA)
- Immunology (AREA)
- Physics & Mathematics (AREA)
- Molecular Biology (AREA)
- Biotechnology (AREA)
- Biophysics (AREA)
- Analytical Chemistry (AREA)
- Biochemistry (AREA)
- Bioinformatics & Cheminformatics (AREA)
- General Engineering & Computer Science (AREA)
- General Health & Medical Sciences (AREA)
- Genetics & Genomics (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
Abstract
Un método para la determinación de peroxidasa en una muestra, fisiológica cuyo método comprende mezclar en una muestra, fisiológica cuyo método comprende mezclar la muestra, en un tampón que proporcione un valor pH de 3 a 10,5, con un substrato para la peroxidasa, con una mezcla de uno o más compuestos de la fórmula general (I): **(Fórmula)** en donde R representa un átomo de hidrógeno o de metal alcalino y R1, R2, R3, R4 y R5 representan, cada uno, un átomo de hidrógeno, bromo o cloro o un grupo nitro, alquílico inferior o carboxílico, y con 4-aminoantipirina, incubar la mezcla resultante a una temperatura comprendida entre 10ºC y 60ºC y determinar el contenido de peroxidasa presente en la muestra midiendo el aumento de intensidad de color por intervalo de tiempo.
Applications Claiming Priority (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
CH294677 | 1977-03-09 |
Publications (1)
Publication Number | Publication Date |
---|---|
ES467667A1 true ES467667A1 (es) | 1979-09-01 |
Family
ID=4245187
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
ES467667A Expired ES467667A1 (es) | 1977-03-09 | 1978-03-08 | Un metodo para la determinacion de peroxidasa en una muestrafisiologica |
Country Status (12)
Country | Link |
---|---|
JP (1) | JPS53110897A (es) |
AU (1) | AU3382778A (es) |
BE (1) | BE864669A (es) |
DE (1) | DE2809755A1 (es) |
DK (1) | DK104578A (es) |
ES (1) | ES467667A1 (es) |
FR (1) | FR2383441A1 (es) |
IL (1) | IL54187A0 (es) |
IT (1) | IT1095456B (es) |
NL (1) | NL7801413A (es) |
NO (1) | NO780822L (es) |
SE (1) | SE7802667L (es) |
Families Citing this family (14)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US4184923A (en) * | 1977-11-03 | 1980-01-22 | Eastman Kodak Company | Reduction of gentisic acid interference in analytical elements |
DE2965849D1 (en) * | 1978-08-18 | 1983-08-18 | Kyowa Hakko Kogyo Kk | Method and test composition for the determination of a substrate for xanthine-oxidase, including a novel xanthine-oxidase and method for the preparation thereof |
JPS56117798A (en) * | 1980-02-21 | 1981-09-16 | Toyo Jozo Co Ltd | Kit for measuring lipoid peroxide |
JPS5783287A (en) * | 1980-11-14 | 1982-05-25 | Kyowa Hakko Kogyo Co Ltd | Elimination of hydrogen peroxide |
DE3125667C2 (de) * | 1981-06-30 | 1986-01-23 | Boehringer Mannheim Gmbh, 6800 Mannheim | Verfahren und Mittel zum Nachweis von Wasserstoffperoxid |
IT1168043B (it) * | 1981-10-22 | 1987-05-20 | Sclavo Inst Sieroterapeut | Cromogeno per la determinazione colorimetrica dei perossidi e metodo impiegante lo stesso |
WO1983003104A1 (en) * | 1982-03-03 | 1983-09-15 | Carter, Timothy, Joseph, Nicholas | Enhanced luminescent and luminometric assay |
US4460684A (en) * | 1982-08-02 | 1984-07-17 | Miles Laboratories, Inc. | Ascorbate-resistant broad range glucose test composition, test device and method |
US4521511A (en) * | 1982-09-22 | 1985-06-04 | Enzyme Technology Company | Catalyzed colorimetric and fluorometric substrates for peroxidase enzyme determinations |
EP0108526A3 (en) * | 1982-11-01 | 1984-07-11 | Beckman Instruments, Inc. | Method and reagent for the determination of peroxide and precursors thereof |
DE3463395D1 (en) * | 1983-02-11 | 1987-06-04 | Nat Res Dev | Enhanced luminescent or luminometric assay |
CA1252703A (en) * | 1984-02-27 | 1989-04-18 | Nutan B. Shah | Diagnostic saliva test for detecting periodontal disease |
US4828983A (en) * | 1986-07-10 | 1989-05-09 | Eastman Kodak Company | Use of phenols and anilines to increase the rate of peroxidase catalyzed oxidation of leuco dyes |
GB2450351B (en) * | 2007-06-20 | 2012-01-18 | Cozart Bioscience Ltd | Monitoring an Immunoassay |
Family Cites Families (1)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
IT986838B (it) * | 1972-05-12 | 1975-01-30 | Sclavo Inst Sieroterapeut | Complesso di reagenti per la deter minazione enzimatica del glucosio sistema glucosio ossidasi perossi dasi con metodo manuale e automati co con lettura a termine o in cinetica |
-
1978
- 1978-02-07 NL NL7801413A patent/NL7801413A/xx not_active Application Discontinuation
- 1978-02-28 IT IT20739/78A patent/IT1095456B/it active
- 1978-03-03 IL IL54187A patent/IL54187A0/xx unknown
- 1978-03-03 AU AU33827/78A patent/AU3382778A/en active Pending
- 1978-03-07 FR FR7806439A patent/FR2383441A1/fr not_active Withdrawn
- 1978-03-07 JP JP2509578A patent/JPS53110897A/ja active Pending
- 1978-03-07 DE DE19782809755 patent/DE2809755A1/de active Pending
- 1978-03-08 NO NO780822A patent/NO780822L/no unknown
- 1978-03-08 SE SE7802667A patent/SE7802667L/xx unknown
- 1978-03-08 DK DK104578A patent/DK104578A/da not_active Application Discontinuation
- 1978-03-08 ES ES467667A patent/ES467667A1/es not_active Expired
- 1978-03-08 BE BE185748A patent/BE864669A/xx unknown
Also Published As
Publication number | Publication date |
---|---|
JPS53110897A (en) | 1978-09-27 |
FR2383441A1 (fr) | 1978-10-06 |
DK104578A (da) | 1978-09-10 |
BE864669A (fr) | 1978-09-08 |
NO780822L (no) | 1978-09-12 |
SE7802667L (sv) | 1978-09-10 |
AU3382778A (en) | 1979-09-06 |
IT7820739A0 (it) | 1978-02-28 |
NL7801413A (nl) | 1978-09-12 |
IL54187A0 (en) | 1978-06-15 |
IT1095456B (it) | 1985-08-10 |
DE2809755A1 (de) | 1978-09-14 |
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