ES355019A1 - Improvements in or relating to the production of nitrogenous material - Google Patents
Improvements in or relating to the production of nitrogenous materialInfo
- Publication number
- ES355019A1 ES355019A1 ES355019A ES355019A ES355019A1 ES 355019 A1 ES355019 A1 ES 355019A1 ES 355019 A ES355019 A ES 355019A ES 355019 A ES355019 A ES 355019A ES 355019 A1 ES355019 A1 ES 355019A1
- Authority
- ES
- Spain
- Prior art keywords
- cells
- hours
- formic acid
- extract
- phase
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Expired
Links
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
- C12P19/00—Preparation of compounds containing saccharide radicals
- C12P19/02—Monosaccharides
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
- C12N1/26—Processes using, or culture media containing, hydrocarbons
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
- C12P19/00—Preparation of compounds containing saccharide radicals
- C12P19/26—Preparation of nitrogen-containing carbohydrates
- C12P19/28—N-glycosides
- C12P19/42—Cobalamins, i.e. vitamin B12, LLD factor
Landscapes
- Chemical & Material Sciences (AREA)
- Organic Chemistry (AREA)
- Life Sciences & Earth Sciences (AREA)
- Engineering & Computer Science (AREA)
- Health & Medical Sciences (AREA)
- Wood Science & Technology (AREA)
- Zoology (AREA)
- Genetics & Genomics (AREA)
- Biotechnology (AREA)
- Bioinformatics & Cheminformatics (AREA)
- General Health & Medical Sciences (AREA)
- Microbiology (AREA)
- General Engineering & Computer Science (AREA)
- Biochemistry (AREA)
- Chemical Kinetics & Catalysis (AREA)
- General Chemical & Material Sciences (AREA)
- Molecular Biology (AREA)
- Oil, Petroleum & Natural Gas (AREA)
- Medicinal Chemistry (AREA)
- Tropical Medicine & Parasitology (AREA)
- Virology (AREA)
- Biomedical Technology (AREA)
- Preparation Of Compounds By Using Micro-Organisms (AREA)
- Peptides Or Proteins (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
Abstract
In the recovery of a nitrogenous product from the cells of micro-organisms, the cells are extracted with a solvent which consists of or contains formic acid, the extraction being carried out under conditions such that a substantial proportion of the free protein content of the cells and of compounds present in the cells having carbohydrate-to-protein bonds pass into the extract phase and such that a substantial proportion of the free carbohydrate content of the cells remains in solid phase, thereafter the extract phase is separated from the solid phase, the extract phase, which contains compounds having carbohydrate-to-protein bonds, is maintained at such temperatures for such a period of time that the bonds are disrupted, and there is recovered from the treated extract phase a nitrogenous product. The micro-organism cells are typically obtained by the aerobic culture of specified normal paraffin-consuming yeasts, moulds or bacteria on a hydrocarbon substrate. Under preferred conditions of operation the micro-organism is in a dry state when subjected to the extraction, formic acid is employed in the anhydrous state or in aqueous dilution containing at least 30% of formic acid and the extraction is carried out at a temperature below 60 C. with a contact time of at least 2 hours. Disruption of the carbohydrate-to-protein bonds may be achieved by subjecting the extract to elevated temperature (60-100 C.) or maintaining the extract for a prolonged period (2-50 hours) at ambient temperature. According to the examples, dry Candida yeast was treated with 85% w./w. aqueous formic acid at 20 C. for 16 hours. The extract phase was maintained under stirring at 20 C. for 48 hours (or at 80 C. for 2 hours) and the formic acid was then removed by vacuum distillation. From the solid residue there was obtained an aqueous phase containing 18% by weight of the original nitrogen of the yeast and a solid phase from which there was obtained by further extraction and precipitation proteinaceous material (12.5% by wt. of nitrogen) constituting 52% by wt. of the original nitrogen of the yeast.
Applications Claiming Priority (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
GB2609367A GB1224172A (en) | 1967-06-06 | 1967-06-06 | Improvements in or relating to the production of nitrogenous material |
Publications (1)
Publication Number | Publication Date |
---|---|
ES355019A1 true ES355019A1 (en) | 1969-11-16 |
Family
ID=10238256
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
ES355019A Expired ES355019A1 (en) | 1967-06-06 | 1968-06-04 | Improvements in or relating to the production of nitrogenous material |
Country Status (4)
Country | Link |
---|---|
BE (1) | BE716165A (en) |
ES (1) | ES355019A1 (en) |
FR (1) | FR1567571A (en) |
GB (1) | GB1224172A (en) |
Families Citing this family (1)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
NO176323C (en) * | 1988-11-18 | 1995-03-15 | Mikalsen Ester | Process for Extraction of Astaxanthin, Related Carotenoids and Astaxanthin Esters from Krill, Shrimp and Other Crustaceans |
-
1967
- 1967-06-06 GB GB2609367A patent/GB1224172A/en not_active Expired
-
1968
- 1968-05-30 FR FR1567571D patent/FR1567571A/fr not_active Expired
- 1968-06-04 ES ES355019A patent/ES355019A1/en not_active Expired
- 1968-06-06 BE BE716165D patent/BE716165A/xx unknown
Also Published As
Publication number | Publication date |
---|---|
BE716165A (en) | 1968-12-06 |
GB1224172A (en) | 1971-03-03 |
FR1567571A (en) | 1969-05-16 |
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