ES2663596T3 - Cepa de algas modificada y método de acumulación de triacilglicerol utilizando dicha cepa - Google Patents

Cepa de algas modificada y método de acumulación de triacilglicerol utilizando dicha cepa Download PDF

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Publication number
ES2663596T3
ES2663596T3 ES14799885.0T ES14799885T ES2663596T3 ES 2663596 T3 ES2663596 T3 ES 2663596T3 ES 14799885 T ES14799885 T ES 14799885T ES 2663596 T3 ES2663596 T3 ES 2663596T3
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Prior art keywords
strain
accumulation method
modified
triacylglycerol accumulation
modified seaweed
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Active
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ES14799885.0T
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English (en)
Inventor
Pierre Calleja
Marina LETERRIER
Eric Marechal
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Centre National de la Recherche Scientifique CNRS
Fermentalg SA
Commissariat a lEnergie Atomique et aux Energies Alternatives CEA
Original Assignee
Centre National de la Recherche Scientifique CNRS
Commissariat a lEnergie Atomique CEA
Fermentalg SA
Commissariat a lEnergie Atomique et aux Energies Alternatives CEA
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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P7/00—Preparation of oxygen-containing organic compounds
    • C12P7/64—Fats; Fatty oils; Ester-type waxes; Higher fatty acids, i.e. having at least seven carbon atoms in an unbroken chain bound to a carboxyl group; Oxidised oils or fats
    • C12P7/6436—Fatty acid esters
    • C12P7/6445—Glycerides
    • C12P7/6463—Glycerides obtained from glyceride producing microorganisms, e.g. single cell oil
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
    • C12N1/12—Unicellular algae; Culture media therefor
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/10—Transferases (2.)
    • C12N9/1025—Acyltransferases (2.3)
    • C12N9/1029—Acyltransferases (2.3) transferring groups other than amino-acyl groups (2.3.1)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y203/00—Acyltransferases (2.3)
    • C12Y203/01—Acyltransferases (2.3) transferring groups other than amino-acyl groups (2.3.1)
    • C12Y203/01051—1-Acylglycerol-3-phosphate O-acyltransferase (2.3.1.51)

Landscapes

  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Organic Chemistry (AREA)
  • Genetics & Genomics (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Microbiology (AREA)
  • Biochemistry (AREA)
  • Biomedical Technology (AREA)
  • Medicinal Chemistry (AREA)
  • Cell Biology (AREA)
  • Molecular Biology (AREA)
  • Oil, Petroleum & Natural Gas (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • General Chemical & Material Sciences (AREA)
  • Tropical Medicine & Parasitology (AREA)
  • Virology (AREA)
  • Botany (AREA)
  • Physics & Mathematics (AREA)
  • Biophysics (AREA)
  • Plant Pathology (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)

Abstract

Cepa modificada de una especie perteneciente al reino Chromalveolata, en la que se modificó la actividad de la proteína CGI-58 para permitir la acumulación de aceite en la cepa, ventajosamente una acumulación de triacilglicerol, en el que la cepa es una cepa genéticamente modificada en la que la expresión del gen CGI-58 se silencia o atenúa.

Description

imagen1
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Claims (1)

  1. imagen1
ES14799885.0T 2013-07-19 2014-07-21 Cepa de algas modificada y método de acumulación de triacilglicerol utilizando dicha cepa Active ES2663596T3 (es)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US201361856378P 2013-07-19 2013-07-19
US201361856378P 2013-07-19
PCT/IB2014/002252 WO2015008160A2 (en) 2013-07-19 2014-07-21 Modified algae strain and method of triacylglycerol accumulation using said strain

Publications (1)

Publication Number Publication Date
ES2663596T3 true ES2663596T3 (es) 2018-04-16

Family

ID=51905311

Family Applications (1)

Application Number Title Priority Date Filing Date
ES14799885.0T Active ES2663596T3 (es) 2013-07-19 2014-07-21 Cepa de algas modificada y método de acumulación de triacilglicerol utilizando dicha cepa

Country Status (8)

Country Link
US (1) US9938545B2 (es)
EP (1) EP3022290B1 (es)
JP (1) JP6568061B2 (es)
DK (1) DK3022290T3 (es)
ES (1) ES2663596T3 (es)
NO (1) NO3111086T3 (es)
PT (1) PT3022290T (es)
WO (1) WO2015008160A2 (es)

Families Citing this family (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
ITUB20159221A1 (it) * 2015-12-23 2017-06-23 Univ Degli Studi Padova Microalga con migliore accumulo di triacilglicerolo (TAG) mediante modificazione genetica di trasportatori/translocatori di trioso fosfati (TPT)
AU2017213137A1 (en) * 2016-01-29 2018-08-02 Total Raffinage Chimie Increased triacylglycerol production in microalgae
FR3085962B1 (fr) 2018-09-14 2021-06-18 Fermentalg Procede d'extracton d'une huile riche en pufa

Family Cites Families (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US8507754B2 (en) * 2009-01-31 2013-08-13 University Of North Texas Engineering lipids in vegetative tissues of plants
US9868959B2 (en) * 2010-12-13 2018-01-16 J. Craig Venter Institute Engineered microalgae with enhanced lipid production
DE102011113283A1 (de) * 2011-09-06 2013-03-07 Johann Wolfgang Goethe-Universität Erhöhung des Lipidgehaltes in Mikroalgen durch genetische Manipulation einer Triacylglycerin (TAG)-Lipase
MY188956A (en) * 2011-12-27 2022-01-14 Commw Scient Ind Res Org Processes for producing lipids
US8809026B2 (en) * 2011-12-27 2014-08-19 Commonwealth Scientific And Industrial Research Organisation Processes for producing lipids

Also Published As

Publication number Publication date
US9938545B2 (en) 2018-04-10
JP6568061B2 (ja) 2019-08-28
EP3022290A2 (en) 2016-05-25
WO2015008160A3 (en) 2015-11-19
PT3022290T (pt) 2018-04-02
NO3111086T3 (es) 2018-06-02
DK3022290T3 (en) 2018-03-26
EP3022290B1 (en) 2018-01-03
JP2016527883A (ja) 2016-09-15
US20160160247A1 (en) 2016-06-09
WO2015008160A2 (en) 2015-01-22

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