ES2608313T3 - Identificación de resistencia antibiótica en microorganismos - Google Patents

Identificación de resistencia antibiótica en microorganismos Download PDF

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Publication number
ES2608313T3
ES2608313T3 ES11158836T ES11158836T ES2608313T3 ES 2608313 T3 ES2608313 T3 ES 2608313T3 ES 11158836 T ES11158836 T ES 11158836T ES 11158836 T ES11158836 T ES 11158836T ES 2608313 T3 ES2608313 T3 ES 2608313T3
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Spain
Prior art keywords
bacterial cell
nucleic acid
individual bacterial
marker
selectively
Prior art date
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Active
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ES11158836T
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English (en)
Inventor
Walter Freiherr Von Stein
Mirko Stange
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miacom Diagnostics GmbH
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miacom Diagnostics GmbH
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Publication date
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Publication of ES2608313T3 publication Critical patent/ES2608313T3/es
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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6876—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes
    • C12Q1/6888—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for detection or identification of organisms
    • C12Q1/689—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for detection or identification of organisms for bacteria
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K16/00—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies
    • C07K16/40—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies against enzymes
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/569—Immunoassay; Biospecific binding assay; Materials therefor for microorganisms, e.g. protozoa, bacteria, viruses
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2600/00—Oligonucleotides characterized by their use
    • C12Q2600/106—Pharmacogenomics, i.e. genetic variability in individual responses to drugs and drug metabolism
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2600/00—Oligonucleotides characterized by their use
    • C12Q2600/136—Screening for pharmacological compounds
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N2333/00—Assays involving biological materials from specific organisms or of a specific nature
    • G01N2333/195—Assays involving biological materials from specific organisms or of a specific nature from bacteria
    • G01N2333/305—Assays involving biological materials from specific organisms or of a specific nature from bacteria from Micrococcaceae (F)
    • G01N2333/31—Assays involving biological materials from specific organisms or of a specific nature from bacteria from Micrococcaceae (F) from Staphylococcus (G)
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N2800/00—Detection or diagnosis of diseases
    • G01N2800/44—Multiple drug resistance

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  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Organic Chemistry (AREA)
  • Engineering & Computer Science (AREA)
  • Immunology (AREA)
  • Analytical Chemistry (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Molecular Biology (AREA)
  • General Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Biotechnology (AREA)
  • Biophysics (AREA)
  • Microbiology (AREA)
  • Physics & Mathematics (AREA)
  • Genetics & Genomics (AREA)
  • Biomedical Technology (AREA)
  • Urology & Nephrology (AREA)
  • Medicinal Chemistry (AREA)
  • Hematology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • Virology (AREA)
  • Cell Biology (AREA)
  • Tropical Medicine & Parasitology (AREA)
  • Food Science & Technology (AREA)
  • General Physics & Mathematics (AREA)
  • Pathology (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)

Abstract

Método para la identificación simultánea de una célula bacteriana individual y la detección de una resistencia antibiótica en dicha célula bacteriana individual en una muestra biológica, que comprende las etapas: (a) poner en contacto dicha célula bacteriana individual con un primer ácido nucleico capaz de hibridarse selectivamente con un ácido nucleico en dicha célula bacteriana en condiciones in situ, en donde el primer ácido nucleico se hibrida selectivamente con el ácido nucleico, y en el que el primer ácido nucleico se marca con un primer marcador, (b) identificar la célula bacteriana por la detección de la presencia del primer marcador en dicha célula bacteriana individual, (c) poner en contacto dicha misma célula bacteriana individual con al menos una sonda para la detección de una resistencia antibiótica en la célula bacteriana en las mismas dichas condiciones in situ, en el que dicha al menos una sonda se selecciona de (i) anticuerpos y fragmentos de los mismos, siendo capaces los anticuerpos y fragmentos de los mismos de unirse selectivamente a un factor de resistencia, y en el que el anticuerpo y los fragmentos de los mismos se marcan con un segundo marcador, en donde dicha célula bacteriana individual se pone en contacto con el anticuerpo y/o dicho fragmento del mismo en condiciones en donde el anticuerpo y/o el fragmento del mismo se une selectivamente al factor de resistencia, (ii) segundos ácidos nucleicos capaces de hibridarse con un ARN que codifica para un factor de resistencia, en el que el segundo ácido nucleico se marca con un tercer marcador, en donde dicha célula bacteriana individual se pone en contacto con el segundo ácido nucleico bajo condiciones en las que el segundo ácido nucleico se hibrida selectivamente con el ARN, (iii) knottins, proteínas cistina-nudo o/y aptámeros capaces de unirse selectivamente a un factor de resistencia, y en el que los knottins, las proteínas cistina-nudo o/y los aptámeros se marcan con un cuarto marcador, en donde dicha célula bacteriana individual se pone en contacto con el knottin, proteína de cistina-nudo y/o aptámero en condiciones en las que el knottin, la proteína de cistina- nudo y/o el aptámero se unen selectivamente al factor de resistencia, y (iv) nitrocefina, en donde dicha célula bacteriana individual se pone en contacto con dicha nitrocefina en condiciones en las que beta-lactamasa modifica a dicha nitrocefina, y (d) determinar la resistencia a los antibióticos de la célula bacteriana por la detección de la presencia de uno o más marcadores, el segundo marcador, el tercer marcador, el cuarto marcador, o/y la nitrocefina modificada en la célula bacteriana individual, en donde las etapas (b) y (d) se realizan simultáneamente en dicha célula bacteriana individual.
ES11158836T 2011-03-18 2011-03-18 Identificación de resistencia antibiótica en microorganismos Active ES2608313T3 (es)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
EP11158836.4A EP2500435B1 (en) 2011-03-18 2011-03-18 Identification of antibiotic resistance in micro organisms

Publications (1)

Publication Number Publication Date
ES2608313T3 true ES2608313T3 (es) 2017-04-07

Family

ID=45952473

Family Applications (1)

Application Number Title Priority Date Filing Date
ES11158836T Active ES2608313T3 (es) 2011-03-18 2011-03-18 Identificación de resistencia antibiótica en microorganismos

Country Status (4)

Country Link
US (1) US20140295417A1 (es)
EP (1) EP2500435B1 (es)
ES (1) ES2608313T3 (es)
WO (1) WO2012126882A1 (es)

Families Citing this family (9)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
KR102257912B1 (ko) * 2013-03-13 2021-05-27 메소 스케일 테크놀러지즈, 엘엘시 개선된 분석 방법
US10114015B2 (en) 2013-03-13 2018-10-30 Meso Scale Technologies, Llc. Assay methods
CN105473740B (zh) 2013-07-03 2021-04-09 克维拉公司 靶向抗生素敏感性测试的方法
CN103645273B (zh) * 2013-12-12 2015-12-30 宁波诺威医疗器械有限公司 β-内酰胺类抗生素耐药性的检测方法、***及其应用
EP3143401A4 (en) 2014-05-15 2017-10-11 Meso Scale Technologies, LLC Improved assay methods
CN105695598A (zh) * 2016-03-25 2016-06-22 苏州达麦迪生物医学科技有限公司 用于耐甲氧西林金黄色葡萄球菌检测的分子信标探针、试剂盒及方法
EP3737770A4 (en) 2018-01-10 2022-02-09 Selux Diagnostics Inc. ASSAYS TO IMPROVE THE ACCURACY OF AN AUTOMATED ANTIMICROBIAL SUSCEPTIBILITY TEST
EP3556862A1 (en) * 2018-04-17 2019-10-23 Universitat Autonoma de Barcelona Rapid detection of antibiotic resistance by bacterial ribosome immunoprecipitation
CN109486739B (zh) * 2018-11-23 2022-02-01 上海海洋大学 一种诱导副溶血性弧菌产生左氧氟沙星耐药性的方法

Family Cites Families (11)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20040242521A1 (en) * 1999-10-25 2004-12-02 Board Of Regents, The University Of Texas System Thio-siRNA aptamers
CN100354430C (zh) * 2001-09-06 2007-12-12 基因描绘***有限公司 一种检测样品中靶细胞或病毒的方法
AU2003225629A1 (en) * 2002-02-27 2003-09-09 University Of Delaware Molecular markers for identification of fat and lean phenotypes in chickens
WO2004097417A1 (en) * 2003-05-02 2004-11-11 Canon Kabushiki Kaisha Structured construct and producing method therefor
WO2008002600A2 (en) * 2006-06-26 2008-01-03 The Regents Of The University Of California Biosynthesis of salinosporamide a and analogs and methods thereof
DK2097541T3 (da) 2006-10-10 2013-03-18 Miacom Diagnostics Gmbh Nukleinsyre-beacons til fluorescent in-situ hybridisering og chip-teknologi
EP2245178B1 (en) * 2008-02-01 2014-12-24 miacom Diagnostics GmbH Identification of antibiotic resistance using labelled antibiotics
US8735554B2 (en) * 2008-05-07 2014-05-27 Innovative Biosensors, Inc. Reagents, methods, and systems for detecting methicillin-resistant Staphylococcus
WO2010048511A1 (en) * 2008-10-24 2010-04-29 Becton, Dickinson And Company Antibiotic susceptibility profiling methods
US8609024B2 (en) * 2009-05-15 2013-12-17 Biomerieux, Inc. System and method for automatically venting and sampling a culture specimen container
US9279160B2 (en) * 2009-06-22 2016-03-08 Statens Serum Institut DNA-based methods for clone-specific identification of staphylococcus aureus

Also Published As

Publication number Publication date
EP2500435A1 (en) 2012-09-19
WO2012126882A1 (en) 2012-09-27
EP2500435B1 (en) 2016-09-21
US20140295417A1 (en) 2014-10-02

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