ES2571584T3 - Preparación de hialuronato de sodio usando un mutante de Streptococcus zooepidemicus - Google Patents

Preparación de hialuronato de sodio usando un mutante de Streptococcus zooepidemicus

Info

Publication number
ES2571584T3
ES2571584T3 ES13734680T ES13734680T ES2571584T3 ES 2571584 T3 ES2571584 T3 ES 2571584T3 ES 13734680 T ES13734680 T ES 13734680T ES 13734680 T ES13734680 T ES 13734680T ES 2571584 T3 ES2571584 T3 ES 2571584T3
Authority
ES
Spain
Prior art keywords
sodium hyaluronate
mutant
preparation
streptococcus zooepidemicus
barg
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Active
Application number
ES13734680T
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English (en)
Inventor
Maurizio Pagliuca
Alessandro Ruggiero
Felice Volpe
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
ALTERGON ITALIA Srl
Original Assignee
ALTERGON ITALIA Srl
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
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Publication of ES2571584T3 publication Critical patent/ES2571584T3/es
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Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P19/00—Preparation of compounds containing saccharide radicals
    • C12P19/26—Preparation of nitrogen-containing carbohydrates
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
    • C12N1/20—Bacteria; Culture media therefor
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
    • C12N1/20—Bacteria; Culture media therefor
    • C12N1/205—Bacterial isolates
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12R—INDEXING SCHEME ASSOCIATED WITH SUBCLASSES C12C - C12Q, RELATING TO MICROORGANISMS
    • C12R2001/00—Microorganisms ; Processes using microorganisms
    • C12R2001/01—Bacteria or Actinomycetales ; using bacteria or Actinomycetales
    • C12R2001/46—Streptococcus ; Enterococcus; Lactococcus

Landscapes

  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Organic Chemistry (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Genetics & Genomics (AREA)
  • Biotechnology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Microbiology (AREA)
  • Biochemistry (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • General Chemical & Material Sciences (AREA)
  • Molecular Biology (AREA)
  • Medicinal Chemistry (AREA)
  • Tropical Medicine & Parasitology (AREA)
  • Virology (AREA)
  • Biomedical Technology (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Polysaccharides And Polysaccharide Derivatives (AREA)
  • Pharmaceuticals Containing Other Organic And Inorganic Compounds (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)

Abstract

Procedimiento de fabricación de hialuronato sódico con un peso molecular definido que varía de 60 a 2.400 kDa y polidispersidad baja (1,4 Mw / Mn) que comprende: a) una etapa de fermentación de Streptococcus equi subspecie zooepidemicus CNCM 1-4645 en un medio de cultivo adecuado; b) una etapa de ultrafiltración de la solución filtrada acelular, concentración y diafiltración de la solución en condiciones de presión diferencial (ΔP) de 1,0 a 5,0 barg y a una presión transmembrana (PTM) de 0,5 a 4,5 barg.
ES13734680T 2012-07-05 2013-06-14 Preparación de hialuronato de sodio usando un mutante de Streptococcus zooepidemicus Active ES2571584T3 (es)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
IT001184A ITMI20121184A1 (it) 2012-07-05 2012-07-05 Processo per la produzione industriale di ialuronato di sodio (hana) altamente purificato a peso molecolare controllato
PCT/EP2013/062360 WO2014005822A1 (en) 2012-07-05 2013-06-14 Production of highly purified sodium hyaluronate (hana) with controlled molecular weight

Publications (1)

Publication Number Publication Date
ES2571584T3 true ES2571584T3 (es) 2016-05-26

Family

ID=46982672

Family Applications (1)

Application Number Title Priority Date Filing Date
ES13734680T Active ES2571584T3 (es) 2012-07-05 2013-06-14 Preparación de hialuronato de sodio usando un mutante de Streptococcus zooepidemicus

Country Status (10)

Country Link
US (1) US9347079B2 (es)
EP (1) EP2870255B1 (es)
CN (1) CN104662158B (es)
CA (1) CA2879538C (es)
ES (1) ES2571584T3 (es)
HK (1) HK1206788A1 (es)
HU (1) HUE029596T2 (es)
IT (1) ITMI20121184A1 (es)
PL (1) PL2870255T3 (es)
WO (1) WO2014005822A1 (es)

Families Citing this family (14)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN105949478B (zh) * 2016-05-05 2021-10-01 宜昌东阳光生化制药有限公司 除去交联透明质酸中交联剂的方法
IT201600079633A1 (it) * 2016-07-28 2018-01-28 Fidia Farm Spa Procedimento di preparazione e purificazione del sale sodico dell’acido ialuronico
KR101784864B1 (ko) 2016-10-07 2017-10-12 (주)진우바이오 신규 스트렙토코커스 쥬에피데미쿠스 kdk0001 및 이로부터 고분자 히알루론산을 생산하는 방법
CN106834387A (zh) * 2017-02-12 2017-06-13 江苏诚信药业有限公司 一种制备玻尿酸的方法
GB201703850D0 (en) * 2017-03-10 2017-04-26 Givaudan Sa Improvements in or relating to organic compounds
CN107338201B (zh) * 2017-06-23 2021-02-02 浙江工业大学 马链球菌兽疫亚种sxy36及在发酵生产透明质酸中的应用
IT201700081449A1 (it) * 2017-07-18 2019-01-18 Fidia Farm Spa Procedimento di purificazione dell'acido ialuronico
WO2019097427A1 (en) * 2017-11-17 2019-05-23 Altergon Sa Resorbable implantable devices based on crosslinked glycosaminoglycans, and process for the preparation thereof
CN108611387B (zh) * 2018-05-07 2020-09-08 山东焦点生物科技股份有限公司 一种利用植物蛋白胨生产医药级透明质酸钠的方法
CN108992369B (zh) * 2018-07-02 2021-07-30 山东天晟生物科技有限公司 一种透明质酸、其制备方法和用途
IT201800007683A1 (it) 2018-07-31 2020-01-31 Altergon Sa Composizioni cooperative sinergiche utili per aumento del tessuto molle, rilascio di farmaco e campi correlati
CN109295132A (zh) * 2018-10-12 2019-02-01 上海景峰制药有限公司 一种生产玻璃酸钠的培养基及其制备方法
CN109161571A (zh) * 2018-10-12 2019-01-08 上海景峰制药有限公司 一种用于生产透明质酸钠的培养基及其制备方法
CN109439584A (zh) * 2018-11-15 2019-03-08 广东省农业科学院农业生物基因研究中心 一种从大肠杆菌发酵液中快速回收大肠杆菌细胞的方法

Family Cites Families (10)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US7091008B1 (en) * 1994-07-01 2006-08-15 The Board Of Regents Of The University Of Oklahoma Hyaluronan synthase genes and expression thereof in Bacillus hosts
AU735695B2 (en) * 1996-10-10 2001-07-12 Neose Technologies, Inc. Carbohydrate purification using ultrafiltration, reverse osmosis and nanofiltration
WO2008035372A2 (en) * 2006-07-06 2008-03-27 Reliance Life Sciences Pvt Ltd Efficient process for purification of high molecular weight hyaluronic acid
CA2732092C (en) * 2008-07-25 2015-02-24 Denki Kagaku Kogyo Kabushiki Kaisha Method for producing hyaluronic acid
US20100137579A1 (en) * 2008-12-01 2010-06-03 Sustineo Biotechnology Co., Ltd. Process for purifying medical grade hyaluronic acid
CN101550199B (zh) * 2009-05-09 2013-05-15 山东众山生物科技有限公司 一种从透明质酸发酵液中制备透明质酸钠的方法
CN101935678B (zh) * 2009-06-30 2014-03-26 上海佰加壹医药有限公司 一种生产透明质酸发酵液的方法
SK50752009A3 (sk) * 2009-12-17 2011-07-06 Contipro C A S Geneticky upravený kmeň Streptococcus equi subsp. zooepidemicus produkujúci vo zvýšenej miere kyselinu hyalúronovú
JP2012191901A (ja) * 2011-03-17 2012-10-11 Mitsubishi Rayon Co Ltd 撹拌型培養槽
CN102242165A (zh) * 2011-05-26 2011-11-16 上海应用技术学院 一种发酵生产高分子量透明质酸钠的方法及其所用的培养基

Also Published As

Publication number Publication date
EP2870255A1 (en) 2015-05-13
CN104662158B (zh) 2018-02-16
CA2879538C (en) 2019-12-31
WO2014005822A1 (en) 2014-01-09
CA2879538A1 (en) 2014-01-09
HK1206788A1 (zh) 2016-01-15
HUE029596T2 (en) 2017-03-28
ITMI20121184A1 (it) 2014-01-06
PL2870255T3 (pl) 2016-07-29
US20150152459A1 (en) 2015-06-04
CN104662158A (zh) 2015-05-27
EP2870255B1 (en) 2016-04-06
US9347079B2 (en) 2016-05-24

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