ES2527684T3 - Detección mejorada de contaminación bacteriana (mollicutes) - Google Patents
Detección mejorada de contaminación bacteriana (mollicutes) Download PDFInfo
- Publication number
- ES2527684T3 ES2527684T3 ES10740163.0T ES10740163T ES2527684T3 ES 2527684 T3 ES2527684 T3 ES 2527684T3 ES 10740163 T ES10740163 T ES 10740163T ES 2527684 T3 ES2527684 T3 ES 2527684T3
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- Prior art keywords
- sample
- mycoplasma
- absence
- composition
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- Prior art date
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6876—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes
- C12Q1/6888—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for detection or identification of organisms
- C12Q1/689—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for detection or identification of organisms for bacteria
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6844—Nucleic acid amplification reactions
- C12Q1/6848—Nucleic acid amplification reactions characterised by the means for preventing contamination or increasing the specificity or sensitivity of an amplification reaction
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6844—Nucleic acid amplification reactions
- C12Q1/686—Polymerase chain reaction [PCR]
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q2527/00—Reactions demanding special reaction conditions
- C12Q2527/125—Specific component of sample, medium or buffer
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q2527/00—Reactions demanding special reaction conditions
- C12Q2527/137—Concentration of a component of medium
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q2549/00—Reactions characterised by the features used to influence the efficiency or specificity
- C12Q2549/10—Reactions characterised by the features used to influence the efficiency or specificity the purpose being that of reducing false positive or false negative signals
- C12Q2549/125—Reactions characterised by the features used to influence the efficiency or specificity the purpose being that of reducing false positive or false negative signals using sterilising/blocking agents, e.g. albumin
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q2600/00—Oligonucleotides characterized by their use
- C12Q2600/158—Expression markers
Landscapes
- Chemical & Material Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Organic Chemistry (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Zoology (AREA)
- Wood Science & Technology (AREA)
- Health & Medical Sciences (AREA)
- Engineering & Computer Science (AREA)
- Analytical Chemistry (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Immunology (AREA)
- Microbiology (AREA)
- Molecular Biology (AREA)
- Biotechnology (AREA)
- Biochemistry (AREA)
- Bioinformatics & Cheminformatics (AREA)
- General Engineering & Computer Science (AREA)
- General Health & Medical Sciences (AREA)
- Genetics & Genomics (AREA)
- Biophysics (AREA)
- Physics & Mathematics (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
Abstract
Un procedimiento mejorado para determinar la presencia o la ausencia de un contaminante bacteriano en una muestra con material biológico, contaminante bacteriano que se selecciona del grupo que consiste en Mycoplasma hyorhinis, Mycoplasma arginini, Mycoplasma pneumoniae, Mycoplasma 5 fermentans, Mycoplasma orale, Mycoplasma pirium, Acholeplasma laidlawii y Spiroplasma mirium, procedimiento que comprende las etapas de (a) procesar la muestra y purificar los ácidos nucleicos de la muestra procesada; seguido de (b) formar una composición para una reacción de amplificación basada en PCR (mezcla de reacción), composición que incluye - un primer cebador de acuerdo con la SEQ ID NO:1, - un segundo cebador de acuerdo con la SEQ ID NO:2 y - los ácidos nucleicos purificados de la etapa (a) o una fracción medida de los mismos como molde; seguido de (c) realizar una reacción en cadena de la polimerasa (PCR) con la composición de la etapa (b); seguido de (d) detectar la presencia o la ausencia de una secuencia objetivo amplificada, en el que la presencia de dicha secuencia objetivo amplificada indica la presencia del contaminante bacteriano en la muestra y la ausencia de dicha secuencia objetivo amplificada indica la ausencia del contaminante bacteriano en la muestra; mejora caracterizada por que en relación con el volumen de la muestra, se añade una cantidad predeterminada de ADN de células CHO a (i) la muestra o (ii) la muestra procesada de la etapa (a), o (iii) a los ácidos nucleicos obtenidos en la etapa (a), o (iv) a la composición de la etapa (b), de modo que el ADN de células CHO añadido reduce la amplificación inespecífica de la etapa (d).
Description
Claims (1)
-
imagen1 imagen2
Applications Claiming Priority (3)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
EP09009965 | 2009-08-01 | ||
EP09009965 | 2009-08-01 | ||
PCT/EP2010/004655 WO2011015312A1 (en) | 2009-08-01 | 2010-07-29 | Improved detection of bacterial (mollicutes) contamination |
Publications (1)
Publication Number | Publication Date |
---|---|
ES2527684T3 true ES2527684T3 (es) | 2015-01-28 |
Family
ID=43085928
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
ES10740163.0T Active ES2527684T3 (es) | 2009-08-01 | 2010-07-29 | Detección mejorada de contaminación bacteriana (mollicutes) |
Country Status (9)
Country | Link |
---|---|
US (4) | US20120129181A1 (es) |
EP (1) | EP2459745B1 (es) |
JP (1) | JP5520376B2 (es) |
CN (1) | CN102471804B (es) |
CA (1) | CA2769043C (es) |
ES (1) | ES2527684T3 (es) |
HK (1) | HK1166820A1 (es) |
SG (1) | SG176706A1 (es) |
WO (1) | WO2011015312A1 (es) |
Families Citing this family (4)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN104411338A (zh) | 2012-04-02 | 2015-03-11 | 现代治疗公司 | 用于产生与人类疾病相关的生物制剂和蛋白质的修饰多核苷酸 |
CN103627781A (zh) * | 2012-08-24 | 2014-03-12 | 华北制药集团新药研究开发有限责任公司 | 一种检测cho培养细胞中支原体污染的试剂盒及其检测方法 |
WO2015034928A1 (en) | 2013-09-03 | 2015-03-12 | Moderna Therapeutics, Inc. | Chimeric polynucleotides |
SG11202000127YA (en) * | 2017-12-08 | 2020-06-29 | Terumo Corp | Method for testing presence or absence of mycoplasma |
Family Cites Families (5)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
WO2007053491A2 (en) * | 2005-10-28 | 2007-05-10 | The Trustees Of Columbia University In The City Of New York | Method and kit for evaluating rna quality |
CN100999763A (zh) * | 2006-01-12 | 2007-07-18 | 张曼 | 一种应用改良引物检测肿瘤中细胞***周期蛋白基因6的方法 |
US20090217399A1 (en) * | 2006-03-17 | 2009-08-27 | Massachusetts Institute Of Technology | Lentiviral Vectors That Provide Improved Expression And Reduced Variegation After Transgenesis |
EP2098599A1 (en) * | 2008-03-05 | 2009-09-09 | Deutsches Krebsforschungszentrum, Stiftung des öffentlichen Rechts | Composition comprising an oligonucleotide mixture for the detection of contaminations in cell cultures |
CN101492741A (zh) * | 2009-01-22 | 2009-07-29 | 中国农业科学院北京畜牧兽医研究所 | 一种定量检测猪肺炎支原体的方法 |
-
2010
- 2010-07-29 JP JP2012522028A patent/JP5520376B2/ja active Active
- 2010-07-29 CA CA2769043A patent/CA2769043C/en active Active
- 2010-07-29 WO PCT/EP2010/004655 patent/WO2011015312A1/en active Application Filing
- 2010-07-29 ES ES10740163.0T patent/ES2527684T3/es active Active
- 2010-07-29 CN CN201080033520.2A patent/CN102471804B/zh active Active
- 2010-07-29 EP EP10740163.0A patent/EP2459745B1/en active Active
- 2010-07-29 SG SG2011090354A patent/SG176706A1/en unknown
-
2012
- 2012-02-01 US US13/364,050 patent/US20120129181A1/en not_active Abandoned
- 2012-07-26 HK HK12107368.5A patent/HK1166820A1/xx unknown
-
2014
- 2014-11-21 US US14/549,647 patent/US20150086990A1/en not_active Abandoned
-
2017
- 2017-06-19 US US15/626,641 patent/US11078545B2/en active Active
-
2021
- 2021-06-28 US US17/360,121 patent/US20210324453A1/en active Pending
Also Published As
Publication number | Publication date |
---|---|
WO2011015312A1 (en) | 2011-02-10 |
US20210324453A1 (en) | 2021-10-21 |
JP5520376B2 (ja) | 2014-06-11 |
US20120129181A1 (en) | 2012-05-24 |
US20150086990A1 (en) | 2015-03-26 |
HK1166820A1 (en) | 2012-11-09 |
CN102471804B (zh) | 2015-03-11 |
CA2769043A1 (en) | 2011-02-10 |
CA2769043C (en) | 2017-09-12 |
US11078545B2 (en) | 2021-08-03 |
EP2459745B1 (en) | 2014-11-12 |
JP2013500702A (ja) | 2013-01-10 |
CN102471804A (zh) | 2012-05-23 |
EP2459745A1 (en) | 2012-06-06 |
US20170292148A1 (en) | 2017-10-12 |
SG176706A1 (en) | 2012-01-30 |
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