ES2520615B2 - Agente bacteriostático para suspensiones bacterianas - Google Patents

Agente bacteriostático para suspensiones bacterianas Download PDF

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ES2520615B2
ES2520615B2 ES201431040A ES201431040A ES2520615B2 ES 2520615 B2 ES2520615 B2 ES 2520615B2 ES 201431040 A ES201431040 A ES 201431040A ES 201431040 A ES201431040 A ES 201431040A ES 2520615 B2 ES2520615 B2 ES 2520615B2
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kaolin
suspensions
weight
glycerin
emulson
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ES2520615A1 (es
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Pedro Luis PELEATO ESTAÚN
Francisco ESPINOSA ESCRIG
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SEIPASA S A
SEIPASA SA
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SEIPASA S A
SEIPASA SA
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Priority to ES201431040A priority Critical patent/ES2520615B2/es
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Priority to US15/031,644 priority patent/US20160262376A1/en
Priority to PCT/ES2015/070510 priority patent/WO2016005634A1/es
Priority to ES15818573T priority patent/ES2832998T3/es
Priority to EP15818573.6A priority patent/EP3167717B1/en
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    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
    • A01N63/00—Biocides, pest repellants or attractants, or plant growth regulators containing microorganisms, viruses, microbial fungi, animals or substances produced by, or obtained from, microorganisms, viruses, microbial fungi or animals, e.g. enzymes or fermentates
    • A01N63/50—Isolated enzymes; Isolated proteins
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
    • A01N63/00—Biocides, pest repellants or attractants, or plant growth regulators containing microorganisms, viruses, microbial fungi, animals or substances produced by, or obtained from, microorganisms, viruses, microbial fungi or animals, e.g. enzymes or fermentates
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
    • A01N25/00—Biocides, pest repellants or attractants, or plant growth regulators, characterised by their forms, or by their non-active ingredients or by their methods of application, e.g. seed treatment or sequential application; Substances for reducing the noxious effect of the active ingredients to organisms other than pests
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
    • A01N25/00—Biocides, pest repellants or attractants, or plant growth regulators, characterised by their forms, or by their non-active ingredients or by their methods of application, e.g. seed treatment or sequential application; Substances for reducing the noxious effect of the active ingredients to organisms other than pests
    • A01N25/02—Biocides, pest repellants or attractants, or plant growth regulators, characterised by their forms, or by their non-active ingredients or by their methods of application, e.g. seed treatment or sequential application; Substances for reducing the noxious effect of the active ingredients to organisms other than pests containing liquids as carriers, diluents or solvents
    • A01N25/04—Dispersions, emulsions, suspoemulsions, suspension concentrates or gels
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
    • A01N31/00—Biocides, pest repellants or attractants, or plant growth regulators containing organic oxygen or sulfur compounds
    • A01N31/02—Acyclic compounds
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
    • A01N37/00—Biocides, pest repellants or attractants, or plant growth regulators containing organic compounds containing a carbon atom having three bonds to hetero atoms with at the most two bonds to halogen, e.g. carboxylic acids
    • A01N37/02—Saturated carboxylic acids or thio analogues thereof; Derivatives thereof
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
    • A01N41/00—Biocides, pest repellants or attractants, or plant growth regulators containing organic compounds containing a sulfur atom bound to a hetero atom
    • A01N41/02—Biocides, pest repellants or attractants, or plant growth regulators containing organic compounds containing a sulfur atom bound to a hetero atom containing a sulfur-to-oxygen double bond
    • A01N41/04—Sulfonic acids; Derivatives thereof
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
    • A01N47/00—Biocides, pest repellants or attractants, or plant growth regulators containing organic compounds containing a carbon atom not being member of a ring and having no bond to a carbon or hydrogen atom, e.g. derivatives of carbonic acid
    • A01N47/40—Biocides, pest repellants or attractants, or plant growth regulators containing organic compounds containing a carbon atom not being member of a ring and having no bond to a carbon or hydrogen atom, e.g. derivatives of carbonic acid the carbon atom having a double or triple bond to nitrogen, e.g. cyanates, cyanamides
    • A01N47/42—Biocides, pest repellants or attractants, or plant growth regulators containing organic compounds containing a carbon atom not being member of a ring and having no bond to a carbon or hydrogen atom, e.g. derivatives of carbonic acid the carbon atom having a double or triple bond to nitrogen, e.g. cyanates, cyanamides containing —N=CX2 groups, e.g. isothiourea
    • A01N47/44—Guanidine; Derivatives thereof
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
    • A01N63/00—Biocides, pest repellants or attractants, or plant growth regulators containing microorganisms, viruses, microbial fungi, animals or substances produced by, or obtained from, microorganisms, viruses, microbial fungi or animals, e.g. enzymes or fermentates
    • A01N63/20—Bacteria; Substances produced thereby or obtained therefrom
    • A01N63/22—Bacillus
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
    • A01N65/00—Biocides, pest repellants or attractants, or plant growth regulators containing material from algae, lichens, bryophyta, multi-cellular fungi or plants, or extracts thereof

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  • Life Sciences & Earth Sciences (AREA)
  • General Health & Medical Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Dentistry (AREA)
  • Wood Science & Technology (AREA)
  • Plant Pathology (AREA)
  • Agronomy & Crop Science (AREA)
  • Environmental Sciences (AREA)
  • Pest Control & Pesticides (AREA)
  • Biotechnology (AREA)
  • Microbiology (AREA)
  • Virology (AREA)
  • Toxicology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Mycology (AREA)
  • Dispersion Chemistry (AREA)
  • Chemical & Material Sciences (AREA)
  • Agricultural Chemicals And Associated Chemicals (AREA)

Abstract

Agente bacteriostático para suspensiones bacterianas. Se describe una composición bacteriostática para la fabricación de suspensiones bacterianas o suspensiones de proteínas y el uso de las citadas suspensiones en formulaciones biocidas y/o fitosanitarios verdes que comprenden una disolución acuosa de un poliol y un agente dispersante en donde la relación poliol/agente dispersante está comprendida entre 30:1 (peso/peso) y 110:1 (peso/peso). Se describen suspensiones con un sólido inerte con un tamaño de partícula menor a 40 micras y formulaciones biocidas que comprende un material biológico como proteínas y cepas bacterianas. Se describen formulaciones biocidas de Bacillus subtillis y cistatina.

Description

DESCRIPCIÓN
imagen1
Formulación biocida libre de agentes conservantes
Campo técnico de la invención
La presente invención describe formulación biocida libre de agentes conservantes.
5 Antecedentes de la invención
En los últimos años existe un interés por el desarrollo de productos fitosanitarios respetuosos con el medio ambiente que no contenga componentes tóxicos para el ser humano ni para el ecosistema, desarrollándose los biocidas verdes. Entre los biocidas verdes destacan las diferentes familias, especies y cepas de bacterias con actividad biocida, como por ejemplo
10 Bacillus spp., Pseudomonas spp. o levaduras. Asimismo, se ha descrito la actividad biocida de diferentes proteínas con propiedades insecticidas como por ejemplo las cistatinas.
El material biológico se almacena adsorbido en sustratos inertes, tales como el caolín, sílice, zeolitas, óxidos de zinc, arcillas, tierra de diatomeas, polisacáridos o carbonatos.
Para el uso de los microorganismos en formulaciones líquidas es necesario formular una 15 suspensión estable, es decir, que con el tiempo no se produzcan decantaciones o precipitaciones del material inerte.
Es conocido que las soluciones acuosas deben contener agentes bacteriostáticos o conservantes para evitar la contaminación microbiana. Es conocido el uso de parabenos, azidas o isotiozolina para este fin. Sin embargo, estos productos son tóxicos y contaminantes
20 del medio ambiente y, en consecuencia, no son adecuados para ser usados en “biocidas /fitosanitarios verdes”.
Por lo expuesto anteriormente, las formulaciones líquidas que comprenden material biológico tienen diferentes inconvenientes:
-Las disoluciones deben ser homogéneas y no deben presentar separación de fases en el 25 tiempo,
-Las formulaciones deben mantener su actividad biocida en el tiempo, es decir, el microorganismo debe ser viable durante al menos dos años,
-Las formulaciones no deben contaminarse con microorganismos exógenos sin usar agentes bactericidas o conservantes.
imagen2
imagen3
imagen4
P201431040
22-06-2015
Los estudios de estabilidad acelerados a 54ºC no produjeron una disminución en la viabilidad de los microrganismos a los 14 días y estos resultados garantizan que la suspensión bacteriana a temperatura ambiente (20ºC) será estable durante al menos 2 años.
Ejemplo 1. Actividad bacteriostática las mezclas poliol/agente dispersante
Se prepararon diferentes muestras variando la relación de poliol/agente dispersante en agua. Las muestras se almacenaron en botella cerrada a 20ºC, durante 6 meses. Se determinaron las unidades formadoras de colonia según el método descrito en la US Pharmacopeia.
Poliol =P
Peso (g) Dispersante=D Peso (g) Agua (g) P/D Agua (%) D (%)
1
Glicerina 50,1 Atlox 4915 1,0 30 50,1 37% 1,2%
2
Glicerina 50,6 Emulson AGPSBV 4 1,2 30 42,2 37% 1,5%
3
Glicerina 52,1 Atlox Metarsperse 500 L 1,2 30 43,4 36% 1,4%
4
Glicerina 50,5 Emulson AG/TP1 1 30 50,5 37% 1,2%
5
Glicerina 50,6 Emulson AGPSBV 4 1,3 30 38,9 37% 1,6%
6
Glicerina 50,9 Emulson AG TRST60 1,3 30 39,2 36% 1,6%
7
Glicerina 50,1 Madeol AG W 90 1,3 30 38,5 37% 1,6%
8
Glicerina 52 Madeol MW 1,3 30 40,0 36% 1,6%
9
Glicerina 50,8 Madeol AG OR 95 0,9 30 56,4 37% 1,1%
10
Emulson AGPSBV 4 1,04
10
Madeol AG W 90 0,03
10
Sorbitol 84 Total 1,07 44 78,1 34% 0,8%
11
Monopropilen glicol 50,1 Madeol AG W 90 1,2 30 41,8 37% 1,5%
imagen5
P201431040
22-06-2015 P201431040
3
Glicerina 52,1 Atlox Metarsperse 500 L 1,2 30 Caolín 1,2
4
Glicerina 50,5 Emulson AG/TP1 1 30 Sílice 1,1
5
Glicerina 50,6 Emulson AGPSBV 4 1,3 30 Caolín 1,0
6
Glicerina 50,9 Emulson AG TRST60 1,3 30 Caolín 1,1
7
Glicerina 50,1 Madeol AG W 90 1,3 30 Caolín 1,3
8
Glicerina 52,0 Madeol MW 1,3 30 Caolín 1,3
9
Glicerina 50,8 Madeol AG OR 95 0,9 30 Caolín 1
10
Emulson AGPSBV 4 1,04
10
Madeol AG W 90 0,03
10
Sorbitol 84 Total 1,07 44 Caolín 0,1
11
Monopropilenglicol 50,1 Madeol AG W 90 1,2 30 Caolín 1,2
12
Glicerina 50 ---------------- 0 30 Caolín 1,1
13
Sorbitol 50,0 Atlox 4915 1 30 Caolín 1
14
Sorbitol 50,6 Emulson AGPSBV 4 1,2 30 Zeolita 1,2
15
Sorbitol 52,1 Atlox Metarsperse 1,2 30 Caolín 1,2
22-06-2015
500 L
16
Sorbitol 50,5 Emulson AG/TP1 1 30 Sílice 1,1
17
Sorbitol 50,6 Emulson AGPSBV 4 1,3 30 Caolín 1,0
18
Sorbitol 50,9 Emulson AG TRST60 1,3 30 Caolín 1,1
19
Sorbitol 50,1 Madeol AG W 90 1,3 30 Caolín 1,3
20
Sorbitol 52,0 Madeol MW 1,3 30 Caolín 1,3
21
Sorbitol 50,8 Madeol AG OR 95 0,9 30 Caolín 1
22
Sorbitol 49 ------------ 0 30 Caolín 1,3
23
Glicerina 1148 Emulson AGPSBV 4 10.0 80 Caolín 1
Las muestras se centrifugaron durante 15 minutos a 1735 rpm con un agitador tipo Cowles.
Los resultados muestran que cuando se utiliza como disperesante naftalensulfonato condesado o policarboxilato sódico, tras a centrifugación no se observa la precipitación de los 5 sólidos dispersados.
La muestras 12 y 22, sin agentes dispersantes, presentaron la precipitación del caolín.
En los demás casos se observó la presencia de una espuma persistente o la presencia de sólidos precipitados
10 Ejemplo 3. Estabilidad la suspensiones en función del contenido de sólidos.
Se prepararon diferentes suspensiones utilizando policarboxilato sódico (Emulson AG PS BV 4) como agente dispersante. Se adicionaron diferentes cantidades de sólido inerte.
P201431040
22-06-2015
Poliol
Peso Dispersante Peso(g) Agua Sólido Peso
(g)
(g)
(g)
(g)
1
Glicerina 50,6 Emulson AGPSBV 4 1,2 30 Caolín 10,1
2
Sorbitol 50,4 Emulson AGPSBV 4 1,2 30 Caolín 5,5
3
Monopropilenglicol 49,5 Emulson AGPSBV 4 1,3 30 Caolín 2,6
4
Glicerina 50,1 Emulson AGPSBV 4 1,3 30 Caolín 20,0
5
Emulson AGPSBV 4 10,03
5
Tween 85 8,57
5
Glicerina 1148 Total 18,6 77,9 Caolín 1,1
No observándose precipitados después de centrifugar durante 15 minutos a 1735 rpm.
Ejemplo 4. Fabricación de las suspensiones bacterianas con actividad biocida
5 0,10 gramos de caolín que contenía 10 10 ufc/g de Bacillus subtilis cepa IAB, obtenida según ES24002726, se disolvió en agua destilada, se adicionaron los agentes dispersantes y se homogenizó la suspensión. Posteriormente, la suspensión se diluyó 100 gramos (cps) con polioles. El pH de la suspensión se ajustó a pH 7 con ácido cítrico. La concentración de la suspensión obtenida fueron 107 ufc/g.
10 Los agentes dispersante y polioles utilizados fueron:
Muestra
Peso agua (g) Agente dispersante Peso agente dispersante(g) Poliol cps 100 g
1
7.5 Emulson AGPSBV 4 1.20 Glicerina
2
6 Madeol AG W 90 1.50 Sorbitol 70%
imagen6
imagen7

Claims (1)

  1. imagen1
ES201431040A 2014-07-10 2014-07-10 Agente bacteriostático para suspensiones bacterianas Active ES2520615B2 (es)

Priority Applications (5)

Application Number Priority Date Filing Date Title
ES201431040A ES2520615B2 (es) 2014-07-10 2014-07-10 Agente bacteriostático para suspensiones bacterianas
US15/031,644 US20160262376A1 (en) 2014-07-10 2015-06-30 Bacteriostatic Agent And Biocidal Formulations
PCT/ES2015/070510 WO2016005634A1 (es) 2014-07-10 2015-06-30 Agente bacteriostático y formulaciones biocidas
ES15818573T ES2832998T3 (es) 2014-07-10 2015-06-30 Agente bacteriostático y formulaciones biocidas
EP15818573.6A EP3167717B1 (en) 2014-07-10 2015-06-30 Bacteriostatic agent and biocide formulations

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
ES201431040A ES2520615B2 (es) 2014-07-10 2014-07-10 Agente bacteriostático para suspensiones bacterianas

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ES2520615A1 ES2520615A1 (es) 2014-11-11
ES2520615B2 true ES2520615B2 (es) 2015-08-07

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ES15818573T Active ES2832998T3 (es) 2014-07-10 2015-06-30 Agente bacteriostático y formulaciones biocidas

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EP (1) EP3167717B1 (es)
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WO (1) WO2016005634A1 (es)

Families Citing this family (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CA3002788A1 (en) * 2015-10-30 2017-05-04 Z Probiotics Inc. Dba Z Bioscience Inc. Probiotic compositions and uses thereof
AR108658A1 (es) * 2016-06-03 2018-09-12 Valent Biosciences Llc Composiciones no acuosas, no oleosas con microbios vivos
AR108659A1 (es) * 2016-06-03 2018-09-12 Valent Biosciences Llc Composiciones no acuosas, no oleosas con bacillus amyloliquefaciens
WO2021240315A1 (en) * 2020-05-27 2021-12-02 Setter Dylan John Probiotic bacillus sanitiser

Family Cites Families (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4000258A (en) * 1972-01-19 1976-12-28 Sandoz, Inc. Liquid compositions of Bacillus thuringiensis
JPH05238913A (ja) * 1992-02-27 1993-09-17 Suntory Ltd 農園芸用殺虫剤
JP5037098B2 (ja) * 2006-11-24 2012-09-26 クミアイ化学工業株式会社 農園芸用組成物
EP2005824A1 (de) * 2007-06-21 2008-12-24 Bayer CropScience AG Wirkstoffsuspensionen in Glycerin
KR20110007168A (ko) * 2008-04-07 2011-01-21 바이엘 크롭사이언스 엘피 안정한 포자-함유 수성 제제
CA2837639A1 (en) * 2011-06-24 2012-12-27 Bio-Energy Ingredients Limited Glycerol compositions and solutions

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Publication number Publication date
EP3167717B1 (en) 2020-09-09
US20160262376A1 (en) 2016-09-15
ES2520615A1 (es) 2014-11-11
EP3167717A4 (en) 2018-04-11
EP3167717A1 (en) 2017-05-17
WO2016005634A1 (es) 2016-01-14
ES2832998T3 (es) 2021-06-14

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