ES2438391B1 - CELLULAR CULTURE DEVICE AND METHOD ASSOCIATED WITH SUCH DEVICE - Google Patents
CELLULAR CULTURE DEVICE AND METHOD ASSOCIATED WITH SUCH DEVICE Download PDFInfo
- Publication number
- ES2438391B1 ES2438391B1 ES201230911A ES201230911A ES2438391B1 ES 2438391 B1 ES2438391 B1 ES 2438391B1 ES 201230911 A ES201230911 A ES 201230911A ES 201230911 A ES201230911 A ES 201230911A ES 2438391 B1 ES2438391 B1 ES 2438391B1
- Authority
- ES
- Spain
- Prior art keywords
- method associated
- study
- cell culture
- different
- cellular culture
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Expired - Fee Related
Links
Classifications
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- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
- B01L3/00—Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
- B01L3/50—Containers for the purpose of retaining a material to be analysed, e.g. test tubes
- B01L3/502—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures
- B01L3/5027—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip
- B01L3/502761—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip specially adapted for handling suspended solids or molecules independently from the bulk fluid flow, e.g. for trapping or sorting beads, for physically stretching molecules
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- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01F—MIXING, e.g. DISSOLVING, EMULSIFYING OR DISPERSING
- B01F33/00—Other mixers; Mixing plants; Combinations of mixers
- B01F33/30—Micromixers
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- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01F—MIXING, e.g. DISSOLVING, EMULSIFYING OR DISPERSING
- B01F35/00—Accessories for mixers; Auxiliary operations or auxiliary devices; Parts or details of general application
- B01F35/80—Forming a predetermined ratio of the substances to be mixed
- B01F35/81—Forming mixtures with changing ratios or gradients
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M23/00—Constructional details, e.g. recesses, hinges
- C12M23/02—Form or structure of the vessel
- C12M23/16—Microfluidic devices; Capillary tubes
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- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
- B01L2200/00—Solutions for specific problems relating to chemical or physical laboratory apparatus
- B01L2200/06—Fluid handling related problems
- B01L2200/0647—Handling flowable solids, e.g. microscopic beads, cells, particles
- B01L2200/0668—Trapping microscopic beads
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- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
- B01L2300/00—Additional constructional details
- B01L2300/06—Auxiliary integrated devices, integrated components
- B01L2300/069—Absorbents; Gels to retain a fluid
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- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
- B01L2300/00—Additional constructional details
- B01L2300/08—Geometry, shape and general structure
- B01L2300/0809—Geometry, shape and general structure rectangular shaped
- B01L2300/0816—Cards, e.g. flat sample carriers usually with flow in two horizontal directions
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
- B01L2300/00—Additional constructional details
- B01L2300/08—Geometry, shape and general structure
- B01L2300/0861—Configuration of multiple channels and/or chambers in a single devices
- B01L2300/0864—Configuration of multiple channels and/or chambers in a single devices comprising only one inlet and multiple receiving wells, e.g. for separation, splitting
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- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
- B01L2300/00—Additional constructional details
- B01L2300/08—Geometry, shape and general structure
- B01L2300/0861—Configuration of multiple channels and/or chambers in a single devices
- B01L2300/0874—Three dimensional network
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
- B01L2300/00—Additional constructional details
- B01L2300/08—Geometry, shape and general structure
- B01L2300/0861—Configuration of multiple channels and/or chambers in a single devices
- B01L2300/0883—Serpentine channels
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- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
- B01L2300/00—Additional constructional details
- B01L2300/08—Geometry, shape and general structure
- B01L2300/0887—Laminated structure
Landscapes
- Chemical & Material Sciences (AREA)
- Health & Medical Sciences (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Zoology (AREA)
- Engineering & Computer Science (AREA)
- Clinical Laboratory Science (AREA)
- Dispersion Chemistry (AREA)
- Organic Chemistry (AREA)
- General Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Wood Science & Technology (AREA)
- General Engineering & Computer Science (AREA)
- Physics & Mathematics (AREA)
- Sustainable Development (AREA)
- Microbiology (AREA)
- Genetics & Genomics (AREA)
- Biotechnology (AREA)
- Biomedical Technology (AREA)
- Biochemistry (AREA)
- Fluid Mechanics (AREA)
- Analytical Chemistry (AREA)
- Hematology (AREA)
- Apparatus Associated With Microorganisms And Enzymes (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
Abstract
Dispositivo de cultivo celular y método asociado a dicho dispositivo.#La presente invención se refiere a un dispositivo encapsulable destinado para su uso en el estudio de cultivos celulares, y compuesto preferentemente de un material plástico donde se localizan diferentes pocillos para el cultivo celular, en el fondo de los cuales se deposita un material gelificado con propiedades mecánicas diferentes en cada pocillo. la invención tiene por objeto, preferentemente, la obtención de sistemas de análisis a gran escala que permitan hacer el estudio de la respuesta de un determinado tipo de células, fármacos, etc. en función de diferentes valores de rigidez del sustrato empleados para cada cultivo.Cell culture device and method associated with said device. # The present invention relates to an encapsulable device intended for use in the study of cell cultures, and preferably composed of a plastic material where different wells for cell culture are located, in the bottom of which a gelled material with different mechanical properties is deposited in each well. The object of the invention is preferably to obtain large-scale analysis systems that allow the study of the response of a certain type of cells, drugs, etc. depending on different values of substrate stiffness used for each crop.
Description
Claims (1)
Priority Applications (2)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
ES201230911A ES2438391B1 (en) | 2012-06-12 | 2012-06-12 | CELLULAR CULTURE DEVICE AND METHOD ASSOCIATED WITH SUCH DEVICE |
PCT/ES2013/000141 WO2013186408A1 (en) | 2012-06-12 | 2013-06-10 | Cell culture device and method associated with said |
Applications Claiming Priority (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
ES201230911A ES2438391B1 (en) | 2012-06-12 | 2012-06-12 | CELLULAR CULTURE DEVICE AND METHOD ASSOCIATED WITH SUCH DEVICE |
Publications (2)
Publication Number | Publication Date |
---|---|
ES2438391A1 ES2438391A1 (en) | 2014-01-16 |
ES2438391B1 true ES2438391B1 (en) | 2014-10-22 |
Family
ID=49757625
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
ES201230911A Expired - Fee Related ES2438391B1 (en) | 2012-06-12 | 2012-06-12 | CELLULAR CULTURE DEVICE AND METHOD ASSOCIATED WITH SUCH DEVICE |
Country Status (2)
Country | Link |
---|---|
ES (1) | ES2438391B1 (en) |
WO (1) | WO2013186408A1 (en) |
Families Citing this family (2)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US20200038861A1 (en) * | 2016-10-07 | 2020-02-06 | The Regents Of The University Of Michigan | Systems and methods for high throughput screening |
CN110732355B (en) * | 2019-09-27 | 2021-09-28 | 东南大学 | Micro-mixing micro-fluidic chip |
Family Cites Families (4)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
GB0426809D0 (en) * | 2004-12-07 | 2005-01-12 | Univ Southampton | Nano-optical diffraction bar-code tagging for biological and chemical applications |
ES2263400B1 (en) * | 2006-05-22 | 2007-08-16 | Ikerlan Centro De Investigaciones Tecnologicas, S. Coop. | FLEXIBLE MICRO-NANOFLUIDIC DEVICES. |
US20110269226A1 (en) * | 2008-08-27 | 2011-11-03 | Agency For Science, Technology And Research | Microfluidic Continuous Flow Device for Culturing Biological Material |
WO2012040579A2 (en) * | 2010-09-23 | 2012-03-29 | President And Fellows Of Harvard College | Molecular screening across defined stiffness matrices |
-
2012
- 2012-06-12 ES ES201230911A patent/ES2438391B1/en not_active Expired - Fee Related
-
2013
- 2013-06-10 WO PCT/ES2013/000141 patent/WO2013186408A1/en active Application Filing
Also Published As
Publication number | Publication date |
---|---|
ES2438391A1 (en) | 2014-01-16 |
WO2013186408A1 (en) | 2013-12-19 |
WO2013186408A8 (en) | 2014-02-06 |
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Legal Events
Date | Code | Title | Description |
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FG2A | Definitive protection |
Ref document number: 2438391 Country of ref document: ES Kind code of ref document: B1 Effective date: 20141022 |
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FD2A | Announcement of lapse in spain |
Effective date: 20210915 |