ES2364833B1 - Método de amplificación genómica del virus west nile. - Google Patents

Método de amplificación genómica del virus west nile. Download PDF

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Publication number
ES2364833B1
ES2364833B1 ES201030126A ES201030126A ES2364833B1 ES 2364833 B1 ES2364833 B1 ES 2364833B1 ES 201030126 A ES201030126 A ES 201030126A ES 201030126 A ES201030126 A ES 201030126A ES 2364833 B1 ES2364833 B1 ES 2364833B1
Authority
ES
Spain
Prior art keywords
west nile
amplification method
amplification
nile virus
virus
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired - Fee Related
Application number
ES201030126A
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English (en)
Other versions
ES2364833A1 (es
Inventor
Ana Vázquez González
Antonio Tenorio Matanzo
Ana Isabel Negredo Antón
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Instituto de Salud Carlos III
Original Assignee
Instituto de Salud Carlos III
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Instituto de Salud Carlos III filed Critical Instituto de Salud Carlos III
Priority to ES201030126A priority Critical patent/ES2364833B1/es
Priority to PCT/ES2011/070047 priority patent/WO2011092362A1/es
Publication of ES2364833A1 publication Critical patent/ES2364833A1/es
Application granted granted Critical
Publication of ES2364833B1 publication Critical patent/ES2364833B1/es
Expired - Fee Related legal-status Critical Current
Anticipated expiration legal-status Critical

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Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/70—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving virus or bacteriophage
    • C12Q1/701—Specific hybridization probes
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/70—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving virus or bacteriophage
    • C12Q1/701—Specific hybridization probes
    • C12Q1/702—Specific hybridization probes for retroviruses

Landscapes

  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Organic Chemistry (AREA)
  • Zoology (AREA)
  • Immunology (AREA)
  • Wood Science & Technology (AREA)
  • Engineering & Computer Science (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Virology (AREA)
  • Molecular Biology (AREA)
  • Microbiology (AREA)
  • Biophysics (AREA)
  • Physics & Mathematics (AREA)
  • Analytical Chemistry (AREA)
  • Biotechnology (AREA)
  • Biochemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)

Abstract

Método de amplificación genómica del virus West Nile. El método se basa en la amplificación de una zona de la región 3? no codificante del virus, preferiblemente mediante PCR en Tiempo Real, gracias al uso de una pareja de cebadores mínimamente degenerados, lo que permite la amplificación de virus West Nile presentes en una muestra de cualquiera de los linajes conocidos. El fragmento amplificado se detecta, preferiblemente, con una sonda específica de la zona, también mínimamente degenerada. La pareja de cebadores degenerados elegidos, así como la sonda preferida, dan lugar a una alta sensibilidad en la detección de todos los linajes y también a una alta especificidad para el virus West Nile.
ES201030126A 2010-01-29 2010-01-29 Método de amplificación genómica del virus west nile. Expired - Fee Related ES2364833B1 (es)

Priority Applications (2)

Application Number Priority Date Filing Date Title
ES201030126A ES2364833B1 (es) 2010-01-29 2010-01-29 Método de amplificación genómica del virus west nile.
PCT/ES2011/070047 WO2011092362A1 (es) 2010-01-29 2011-01-25 Método de amplificación genómica del virus west nile

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
ES201030126A ES2364833B1 (es) 2010-01-29 2010-01-29 Método de amplificación genómica del virus west nile.

Publications (2)

Publication Number Publication Date
ES2364833A1 ES2364833A1 (es) 2011-09-15
ES2364833B1 true ES2364833B1 (es) 2012-09-13

Family

ID=44318706

Family Applications (1)

Application Number Title Priority Date Filing Date
ES201030126A Expired - Fee Related ES2364833B1 (es) 2010-01-29 2010-01-29 Método de amplificación genómica del virus west nile.

Country Status (2)

Country Link
ES (1) ES2364833B1 (es)
WO (1) WO2011092362A1 (es)

Family Cites Families (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP2977470B1 (en) * 2002-10-16 2018-01-03 Gen-Probe Incorporated Compositions and methods for detecting west nile virus

Also Published As

Publication number Publication date
WO2011092362A1 (es) 2011-08-04
ES2364833A1 (es) 2011-09-15

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