ES2364833B1 - Método de amplificación genómica del virus west nile. - Google Patents
Método de amplificación genómica del virus west nile. Download PDFInfo
- Publication number
- ES2364833B1 ES2364833B1 ES201030126A ES201030126A ES2364833B1 ES 2364833 B1 ES2364833 B1 ES 2364833B1 ES 201030126 A ES201030126 A ES 201030126A ES 201030126 A ES201030126 A ES 201030126A ES 2364833 B1 ES2364833 B1 ES 2364833B1
- Authority
- ES
- Spain
- Prior art keywords
- west nile
- amplification method
- amplification
- nile virus
- virus
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Expired - Fee Related
Links
- 230000003321 amplification Effects 0.000 title abstract 4
- 238000003199 nucleic acid amplification method Methods 0.000 title abstract 4
- 238000000034 method Methods 0.000 title abstract 3
- 241000700605 Viruses Species 0.000 title abstract 2
- 241000710886 West Nile virus Species 0.000 abstract 3
- 239000000523 sample Substances 0.000 abstract 3
- 238000001514 detection method Methods 0.000 abstract 1
- 239000012634 fragment Substances 0.000 abstract 1
- 238000003753 real-time PCR Methods 0.000 abstract 1
- 230000035945 sensitivity Effects 0.000 abstract 1
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/70—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving virus or bacteriophage
- C12Q1/701—Specific hybridization probes
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/70—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving virus or bacteriophage
- C12Q1/701—Specific hybridization probes
- C12Q1/702—Specific hybridization probes for retroviruses
Landscapes
- Chemical & Material Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Health & Medical Sciences (AREA)
- Organic Chemistry (AREA)
- Zoology (AREA)
- Immunology (AREA)
- Wood Science & Technology (AREA)
- Engineering & Computer Science (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Virology (AREA)
- Molecular Biology (AREA)
- Microbiology (AREA)
- Biophysics (AREA)
- Physics & Mathematics (AREA)
- Analytical Chemistry (AREA)
- Biotechnology (AREA)
- Biochemistry (AREA)
- Bioinformatics & Cheminformatics (AREA)
- General Engineering & Computer Science (AREA)
- General Health & Medical Sciences (AREA)
- Genetics & Genomics (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
Abstract
Método de amplificación genómica del virus West Nile. El método se basa en la amplificación de una zona de la región 3? no codificante del virus, preferiblemente mediante PCR en Tiempo Real, gracias al uso de una pareja de cebadores mínimamente degenerados, lo que permite la amplificación de virus West Nile presentes en una muestra de cualquiera de los linajes conocidos. El fragmento amplificado se detecta, preferiblemente, con una sonda específica de la zona, también mínimamente degenerada. La pareja de cebadores degenerados elegidos, así como la sonda preferida, dan lugar a una alta sensibilidad en la detección de todos los linajes y también a una alta especificidad para el virus West Nile.
Priority Applications (2)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
ES201030126A ES2364833B1 (es) | 2010-01-29 | 2010-01-29 | Método de amplificación genómica del virus west nile. |
PCT/ES2011/070047 WO2011092362A1 (es) | 2010-01-29 | 2011-01-25 | Método de amplificación genómica del virus west nile |
Applications Claiming Priority (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
ES201030126A ES2364833B1 (es) | 2010-01-29 | 2010-01-29 | Método de amplificación genómica del virus west nile. |
Publications (2)
Publication Number | Publication Date |
---|---|
ES2364833A1 ES2364833A1 (es) | 2011-09-15 |
ES2364833B1 true ES2364833B1 (es) | 2012-09-13 |
Family
ID=44318706
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
ES201030126A Expired - Fee Related ES2364833B1 (es) | 2010-01-29 | 2010-01-29 | Método de amplificación genómica del virus west nile. |
Country Status (2)
Country | Link |
---|---|
ES (1) | ES2364833B1 (es) |
WO (1) | WO2011092362A1 (es) |
Family Cites Families (1)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
EP2977470B1 (en) * | 2002-10-16 | 2018-01-03 | Gen-Probe Incorporated | Compositions and methods for detecting west nile virus |
-
2010
- 2010-01-29 ES ES201030126A patent/ES2364833B1/es not_active Expired - Fee Related
-
2011
- 2011-01-25 WO PCT/ES2011/070047 patent/WO2011092362A1/es active Application Filing
Also Published As
Publication number | Publication date |
---|---|
WO2011092362A1 (es) | 2011-08-04 |
ES2364833A1 (es) | 2011-09-15 |
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Legal Events
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