ES2196140T3 - Procedimientos para reducir la concentracion de arn en una mezcla de material biologico utilizando tierra de diatomeas. - Google Patents
Procedimientos para reducir la concentracion de arn en una mezcla de material biologico utilizando tierra de diatomeas.Info
- Publication number
- ES2196140T3 ES2196140T3 ES96903384T ES96903384T ES2196140T3 ES 2196140 T3 ES2196140 T3 ES 2196140T3 ES 96903384 T ES96903384 T ES 96903384T ES 96903384 T ES96903384 T ES 96903384T ES 2196140 T3 ES2196140 T3 ES 2196140T3
- Authority
- ES
- Spain
- Prior art keywords
- concentration
- rna
- diatome
- mix
- biological material
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Expired - Lifetime
Links
- 239000012620 biological material Substances 0.000 title abstract 4
- 241000206761 Bacillariophyta Species 0.000 title abstract 3
- 238000000034 method Methods 0.000 title abstract 2
- 108091032973 (ribonucleotides)n+m Proteins 0.000 abstract 3
- 239000000463 material Substances 0.000 abstract 3
- 239000000203 mixture Substances 0.000 abstract 3
- 108020004566 Transfer RNA Proteins 0.000 abstract 1
- 239000013612 plasmid Substances 0.000 abstract 1
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
- C12N15/1003—Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor
- C12N15/1017—Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor by filtration, e.g. using filters, frits, membranes
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
- C12N1/08—Reducing the nucleic acid content
Landscapes
- Health & Medical Sciences (AREA)
- Engineering & Computer Science (AREA)
- Life Sciences & Earth Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Genetics & Genomics (AREA)
- Biotechnology (AREA)
- Organic Chemistry (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Biomedical Technology (AREA)
- Wood Science & Technology (AREA)
- Zoology (AREA)
- General Engineering & Computer Science (AREA)
- Molecular Biology (AREA)
- General Health & Medical Sciences (AREA)
- Microbiology (AREA)
- Biochemistry (AREA)
- Virology (AREA)
- Tropical Medicine & Parasitology (AREA)
- Medicinal Chemistry (AREA)
- Analytical Chemistry (AREA)
- Crystallography & Structural Chemistry (AREA)
- Physics & Mathematics (AREA)
- Biophysics (AREA)
- Plant Pathology (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Saccharide Compounds (AREA)
- Processing Of Solid Wastes (AREA)
- Treatment Of Sludge (AREA)
- Preparation Of Compounds By Using Micro-Organisms (AREA)
- Disinfection, Sterilisation Or Deodorisation Of Air (AREA)
- Solid-Sorbent Or Filter-Aiding Compositions (AREA)
- Pharmaceuticals Containing Other Organic And Inorganic Compounds (AREA)
Abstract
ESTA INVENCION TRATA DE UN PROCEDIMIENTO PARA REDUCIR LA CONCENTRACION DE ARN EN UNA MEZCLA DE UN MATERIAL BIOLOGICO, QUE INCLUYE LAS ETAPAS SIGUIENTES: (A) PROPORCIONAR UNA MEZCLA DE UN MATERIAL BIOLOGICO QUE POSEE UNA PRIMERA CONCENTRACION DE ARN; (B) FILTRAR LA MEZCLA A TRAVES DE UN MATERIAL DE TIERRA DE DIATOMEAS PARA PRODUCIR UN FILTRADO QUE POSEE UNA SEGUNDA CONCENTRACION DE ARN, SIENDO ESTA SEGUNDA CONCENTRACION MENOR QUE LA PRIMERA CONCENTRACION; Y (C) RECOGER EL FILTRADO QUE POSEE UNA CONCENTRACION DE ARN REDUCIDA. EN UN ASPECTO, EL MATERIAL DE TIERRA DE DIATOMEAS SIRVE PARA PURIFICAR UN ADN PLASMIDO RECOMBINANTE DE UN COMPONENTE DE ARN EN UNA MEZCLA DE UN MATERIAL BIOLOGICO, Y EN OTRO ASPECTO SIRVE PARA SEPARAR DIFERENTES FORMAS DE ARN SOLUBLE EN UNA MEZCLA DE MATERIAL BIOLOGICO.
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US08/372,236 US5576196A (en) | 1995-01-13 | 1995-01-13 | Process for reducing RNA concentration in a mixture of biological material using diatomaceous earth |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| ES2196140T3 true ES2196140T3 (es) | 2003-12-16 |
Family
ID=23467282
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| ES96903384T Expired - Lifetime ES2196140T3 (es) | 1995-01-13 | 1996-01-03 | Procedimientos para reducir la concentracion de arn en una mezcla de material biologico utilizando tierra de diatomeas. |
Country Status (8)
| Country | Link |
|---|---|
| US (1) | US5576196A (es) |
| EP (2) | EP0802975B1 (es) |
| JP (1) | JP3808506B2 (es) |
| AT (2) | ATE419341T1 (es) |
| DE (2) | DE69637798D1 (es) |
| DK (1) | DK0802975T3 (es) |
| ES (1) | ES2196140T3 (es) |
| WO (1) | WO1996021729A1 (es) |
Families Citing this family (21)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| ATE238321T1 (de) | 1996-02-06 | 2003-05-15 | Roche Diagnostics Gmbh | Verfahren zur herstellung von gereinigter nukleinsäure und deren verwendung |
| FR2746412B1 (fr) * | 1996-03-21 | 1998-06-12 | Rhone Poulenc Rorer Sa | Purification d'adn plasmidique de qualite pharmaceutique |
| US7026468B2 (en) | 1996-07-19 | 2006-04-11 | Valentis, Inc. | Process and equipment for plasmid purification |
| US7807822B2 (en) | 1996-08-01 | 2010-10-05 | Robert Bridenbaugh | Methods for purifying nucleic acids |
| US6268492B1 (en) * | 1998-08-10 | 2001-07-31 | Chiron Corporation | Methods for purifying non-chromosomal nucleic acid molecules from cells |
| US6617108B1 (en) | 1999-07-12 | 2003-09-09 | Technology Licensing Co. Llc | Methods and compositions for biotechnical separations using selective precipitation by compaction agents |
| US20020012990A1 (en) * | 1999-12-22 | 2002-01-31 | Lander Russel Jackson | Process for the scaleable purification of plasmid DNA |
| DE10033991A1 (de) * | 2000-07-12 | 2002-01-24 | Qiagen Gmbh | Verfahren zur Isolierung von Nukleinsäuren |
| US7208271B2 (en) | 2001-11-28 | 2007-04-24 | Applera Corporation | Compositions and methods of selective nucleic acid isolation |
| AU2003267175A1 (en) | 2002-09-13 | 2004-04-30 | Valentis, Inc. | Apparatus and method for preparative scale purification of nucleic acids |
| DE60325773D1 (de) * | 2002-12-23 | 2009-02-26 | Vical Inc | Reinigungsverfahren für plasmid-dna |
| US8501402B2 (en) | 2003-03-24 | 2013-08-06 | Boehringer Ingelheim Rcv Gmbh & Co Kg | Methods and devices for producing biomolecules |
| JP2004000922A (ja) * | 2003-03-26 | 2004-01-08 | Toyobo Co Ltd | 核酸またはタンパク質抽出用シリカ粒子組成物 |
| AU2006211216B2 (en) * | 2005-01-31 | 2011-02-03 | Merck Sharp & Dohme Llc | Purification process for plasmid DNA |
| EP2088196A1 (en) | 2008-02-08 | 2009-08-12 | Boehringer Ingelheim RCV GmbH & Co KG | Methods and devices for producing biomolecules |
| US20110313143A1 (en) * | 2008-12-15 | 2011-12-22 | Life Technologies Corporation | Nucleic acid purification apparatus and method |
| ES2543367T3 (es) * | 2010-07-07 | 2015-08-18 | Diagon Kft. | Procedimiento para el aislamiento específico del contenido de ADN total de gérmenes bacterianos y un kit para dicho fin |
| GB201614050D0 (en) * | 2016-08-17 | 2016-09-28 | Glaxosmithkline Ip Dev Ltd | Method for purifying viral vectors |
| CN110612148A (zh) * | 2017-06-27 | 2019-12-24 | 大金工业株式会社 | 处理由含氟聚合物制造工序产生的水性流体的方法和*** |
| EP3473336B1 (en) * | 2017-10-17 | 2020-12-02 | Sartorius Stedim Biotech GmbH | Diatomaceous earth composition with a low endotoxin content |
| US11453877B2 (en) * | 2019-05-15 | 2022-09-27 | Translate Bio, Inc. | Methods for purification of messenger RNA |
Family Cites Families (8)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| DE3639949A1 (de) * | 1986-11-22 | 1988-06-09 | Diagen Inst Molekularbio | Verfahren zur trennung von langkettigen nukleinsaeuren |
| US5075430A (en) * | 1988-12-12 | 1991-12-24 | Bio-Rad Laboratories, Inc. | Process for the purification of DNA on diatomaceous earth |
| NL8900725A (nl) * | 1989-03-23 | 1990-10-16 | Az Univ Amsterdam | Werkwijze en combinatie van middelen voor het isoleren van nucleinezuur. |
| US5234809A (en) * | 1989-03-23 | 1993-08-10 | Akzo N.V. | Process for isolating nucleic acid |
| MX9202037A (es) * | 1991-05-03 | 1992-11-01 | Becton Dickinson Co | Purificacion por filtracion de adn. |
| US5155018A (en) * | 1991-07-10 | 1992-10-13 | Hahnemann University | Process and kit for isolating and purifying RNA from biological sources |
| EP0555798B1 (en) * | 1992-02-13 | 1999-05-06 | Becton, Dickinson and Company | Hydrated celite and purification of DNA |
| DE59506735D1 (de) * | 1994-06-14 | 1999-10-07 | Invitek Gmbh | Universelles verfahren zur isolierung und reinigung von nukleinsäuren aus extrem geringen mengen sowie sehr stark verunreinigten unterschiedlichsten ausgangsmaterialien |
-
1995
- 1995-01-13 US US08/372,236 patent/US5576196A/en not_active Expired - Lifetime
-
1996
- 1996-01-03 ES ES96903384T patent/ES2196140T3/es not_active Expired - Lifetime
- 1996-01-03 WO PCT/US1996/000247 patent/WO1996021729A1/en active IP Right Grant
- 1996-01-03 AT AT02026162T patent/ATE419341T1/de not_active IP Right Cessation
- 1996-01-03 JP JP52176896A patent/JP3808506B2/ja not_active Expired - Fee Related
- 1996-01-03 EP EP96903384A patent/EP0802975B1/en not_active Expired - Lifetime
- 1996-01-03 DE DE69637798T patent/DE69637798D1/de not_active Expired - Fee Related
- 1996-01-03 AT AT96903384T patent/ATE235547T1/de not_active IP Right Cessation
- 1996-01-03 DK DK96903384T patent/DK0802975T3/da active
- 1996-01-03 EP EP02026162A patent/EP1291421B1/en not_active Expired - Lifetime
- 1996-01-03 DE DE69626957T patent/DE69626957T2/de not_active Expired - Lifetime
Also Published As
| Publication number | Publication date |
|---|---|
| EP1291421A1 (en) | 2003-03-12 |
| DE69626957D1 (de) | 2003-04-30 |
| DE69626957T2 (de) | 2004-03-04 |
| EP0802975A1 (en) | 1997-10-29 |
| EP1291421B1 (en) | 2008-12-31 |
| DE69637798D1 (de) | 2009-02-12 |
| JP3808506B2 (ja) | 2006-08-16 |
| JPH11502403A (ja) | 1999-03-02 |
| DK0802975T3 (da) | 2003-07-14 |
| EP0802975B1 (en) | 2003-03-26 |
| US5576196A (en) | 1996-11-19 |
| ATE419341T1 (de) | 2009-01-15 |
| ATE235547T1 (de) | 2003-04-15 |
| WO1996021729A1 (en) | 1996-07-18 |
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