ES2189631B1 - METHOD FOR THE AMPLIFICATION OF DNA BY SEMI-NESTED PCR IN ONE STEP AND ITS USE IN THE IDENTIFICATION OF PLASMODIUM SPECIES. - Google Patents

METHOD FOR THE AMPLIFICATION OF DNA BY SEMI-NESTED PCR IN ONE STEP AND ITS USE IN THE IDENTIFICATION OF PLASMODIUM SPECIES.

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Publication number
ES2189631B1
ES2189631B1 ES200100568A ES200100568A ES2189631B1 ES 2189631 B1 ES2189631 B1 ES 2189631B1 ES 200100568 A ES200100568 A ES 200100568A ES 200100568 A ES200100568 A ES 200100568A ES 2189631 B1 ES2189631 B1 ES 2189631B1
Authority
ES
Spain
Prior art keywords
dna
amplification
semi
nested pcr
plasmodium species
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired - Fee Related
Application number
ES200100568A
Other languages
Spanish (es)
Other versions
ES2189631A1 (en
Inventor
Antonio Madejon Seiz
Susana Vega Rocha
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Biotools Biotechnological and Medical Laboratories SA
Original Assignee
Biotools Biotechnological and Medical Laboratories SA
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Biotools Biotechnological and Medical Laboratories SA filed Critical Biotools Biotechnological and Medical Laboratories SA
Priority to ES200100568A priority Critical patent/ES2189631B1/en
Publication of ES2189631A1 publication Critical patent/ES2189631A1/en
Application granted granted Critical
Publication of ES2189631B1 publication Critical patent/ES2189631B1/en
Anticipated expiration legal-status Critical
Expired - Fee Related legal-status Critical Current

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Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P19/00—Preparation of compounds containing saccharide radicals
    • C12P19/26—Preparation of nitrogen-containing carbohydrates
    • C12P19/28—N-glycosides
    • C12P19/30—Nucleotides
    • C12P19/34—Polynucleotides, e.g. nucleic acids, oligoribonucleotides
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
    • Y02A—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
    • Y02A50/00—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE in human health protection, e.g. against extreme weather
    • Y02A50/30—Against vector-borne diseases, e.g. mosquito-borne, fly-borne, tick-borne or waterborne diseases whose impact is exacerbated by climate change

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  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Genetics & Genomics (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • Molecular Biology (AREA)
  • General Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • Microbiology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Biomedical Technology (AREA)
  • Biophysics (AREA)
  • Physics & Mathematics (AREA)
  • Immunology (AREA)
  • Analytical Chemistry (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • General Chemical & Material Sciences (AREA)
  • Crystallography & Structural Chemistry (AREA)
  • Plant Pathology (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)

Abstract

Método para la amplificación de ADN mediante semi-nested PCR en un solo paso y su empleo en la identificación de especies de Plasmodium. El método para amplificar mediante una reacción de semi-nested PCR en un solo paso, un fragmento de ADN presente en un ADN diana contenido en una muestra, comprende poner en contacto dicho ADN diana con una mezcla de reacción para la amplificación de ADN que comprende (i) dos iniciadores externos con orientaciones contrarias entre sí, uno de los cuales se encuentra en exceso sobre el otro, y (ii) un iniciador interno que tiene la orientación contraria a la del iniciador externo que se encuentra en exceso y, además, dicho iniciador interno se encuentra también en exceso sobre el iniciador externo que tiene su misma orientación. El método es útil para detectar e identificar patógenos, por ejemplo, especies de Plasmodium que causan malaria en humanos.Method for the amplification of DNA by means of semi-nested PCR in a single step and its use in the identification of Plasmodium species. The method for amplifying by means of a one-step semi-nested PCR reaction, a DNA fragment present in a target DNA contained in a sample, comprises contacting said target DNA with a reaction mixture for the amplification of DNA comprising (i) two external initiators with opposite orientations to each other, one of which is in excess over the other, and (ii) an internal initiator that has the orientation opposite to that of the external initiator that is in excess and, in addition, said internal initiator is also in excess over the external initiator that has its same orientation. The method is useful for detecting and identifying pathogens, for example, Plasmodium species that cause malaria in humans.

ES200100568A 2001-03-12 2001-03-12 METHOD FOR THE AMPLIFICATION OF DNA BY SEMI-NESTED PCR IN ONE STEP AND ITS USE IN THE IDENTIFICATION OF PLASMODIUM SPECIES. Expired - Fee Related ES2189631B1 (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
ES200100568A ES2189631B1 (en) 2001-03-12 2001-03-12 METHOD FOR THE AMPLIFICATION OF DNA BY SEMI-NESTED PCR IN ONE STEP AND ITS USE IN THE IDENTIFICATION OF PLASMODIUM SPECIES.

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
ES200100568A ES2189631B1 (en) 2001-03-12 2001-03-12 METHOD FOR THE AMPLIFICATION OF DNA BY SEMI-NESTED PCR IN ONE STEP AND ITS USE IN THE IDENTIFICATION OF PLASMODIUM SPECIES.

Publications (2)

Publication Number Publication Date
ES2189631A1 ES2189631A1 (en) 2003-07-01
ES2189631B1 true ES2189631B1 (en) 2005-01-01

Family

ID=8497047

Family Applications (1)

Application Number Title Priority Date Filing Date
ES200100568A Expired - Fee Related ES2189631B1 (en) 2001-03-12 2001-03-12 METHOD FOR THE AMPLIFICATION OF DNA BY SEMI-NESTED PCR IN ONE STEP AND ITS USE IN THE IDENTIFICATION OF PLASMODIUM SPECIES.

Country Status (1)

Country Link
ES (1) ES2189631B1 (en)

Family Cites Families (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
ES2091976T3 (en) * 1991-06-20 1996-11-16 Hoffmann La Roche PERFECTED METHODS FOR THE AMPLIFICATION OF NUCLEIC ACID.
US5340728A (en) * 1992-12-09 1994-08-23 E. I. Du Pont De Nemours And Company Method for amplification of targeted segments of nucleic acid using nested polymerase chain reaction
AUPO188396A0 (en) * 1996-08-26 1996-09-19 Insearch Limited Detection of protozoan parasites

Non-Patent Citations (2)

* Cited by examiner, † Cited by third party
Title
RUBIO et al. Semi-nested, multiplex polymerase chain reaction for detection of human malaria parasites and evidence of Plasmodium vivax infection in equatorial guinea. Am. J. Trop. Med. Hyg., Febrero 1999. Vol. 60, páginas 183-187. *
SINGH et al. A genus and species specific nested polymerase chain reaction malaria detection assay for epidemiologic studies. Am. J. Trop. Med. Hyg., Abril 1999. Vol. 60, páginas 687-692. *

Also Published As

Publication number Publication date
ES2189631A1 (en) 2003-07-01

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