ES2186768T3 - Procedimiento para la purificacion de acidos nucleicos cortos. - Google Patents
Procedimiento para la purificacion de acidos nucleicos cortos.Info
- Publication number
- ES2186768T3 ES2186768T3 ES96901592T ES96901592T ES2186768T3 ES 2186768 T3 ES2186768 T3 ES 2186768T3 ES 96901592 T ES96901592 T ES 96901592T ES 96901592 T ES96901592 T ES 96901592T ES 2186768 T3 ES2186768 T3 ES 2186768T3
- Authority
- ES
- Spain
- Prior art keywords
- oligonucleotide
- pct
- purification
- adsorbent
- sec
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Expired - Lifetime
Links
Classifications
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07H—SUGARS; DERIVATIVES THEREOF; NUCLEOSIDES; NUCLEOTIDES; NUCLEIC ACIDS
- C07H21/00—Compounds containing two or more mononucleotide units having separate phosphate or polyphosphate groups linked by saccharide radicals of nucleoside groups, e.g. nucleic acids
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
- C12N15/1003—Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor
- C12N15/1006—Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor by means of a solid support carrier, e.g. particles, polymers
- C12N15/101—Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor by means of a solid support carrier, e.g. particles, polymers by chromatography, e.g. electrophoresis, ion-exchange, reverse phase
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N30/00—Investigating or analysing materials by separation into components using adsorption, absorption or similar phenomena or using ion-exchange, e.g. chromatography or field flow fractionation
- G01N30/02—Column chromatography
Landscapes
- Chemical & Material Sciences (AREA)
- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Engineering & Computer Science (AREA)
- Genetics & Genomics (AREA)
- Organic Chemistry (AREA)
- Biochemistry (AREA)
- Molecular Biology (AREA)
- Biotechnology (AREA)
- Biomedical Technology (AREA)
- Analytical Chemistry (AREA)
- General Health & Medical Sciences (AREA)
- General Engineering & Computer Science (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Wood Science & Technology (AREA)
- Zoology (AREA)
- Crystallography & Structural Chemistry (AREA)
- Physics & Mathematics (AREA)
- Biophysics (AREA)
- Plant Pathology (AREA)
- Microbiology (AREA)
- Saccharide Compounds (AREA)
- Treatment Of Sludge (AREA)
Abstract
METODO PARA LA PURIFICACION DE UN OLIGONUCLEOTIDO SINTETICO A PARTIR DE SECUENCIAS ERRONEAS QUE CONSISTE EN PONER EN CONTACTO UNA MUESTRA QUE CONTENGA EL OLIGONUCLEOTIDO DESEADO EN FORMA SOLUBLE AL AGUA PROTEGIDA CON UN ADSORBENTE HIDROFILO QUE CONTENGA GRUPOS DE INTERCAMBIO DE ANIONES BAJO CONDICIONES QUE PERMITAN EL ENLACE DE DICHO OLIGONUCLEOTIDO A DICHO ADSORBENTE, TRAS LO CUAL SE DESPROTEGE EL OLIGONUCLEOTIDO ADSORBIDO Y SE SEPARA DE LAS SECUENCIAS ERRONEAS. LA CARACTERISTICA PARTICULAR DEL METODO ES LA UTILIZACION DE UN ADSORBENTE DE INTERCAMBIO DE IONES QUE SE UNA AL OLIGONUCLEOTIDO PROTEGIDO BAJO CONDICIONES TANTO DE ALTA COMO DE BAJA RESISTENCIA IONICA.
Applications Claiming Priority (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
SE9500183A SE9500183D0 (sv) | 1995-01-20 | 1995-01-20 | Method for the purifivation of short nucleic acids |
Publications (1)
Publication Number | Publication Date |
---|---|
ES2186768T3 true ES2186768T3 (es) | 2003-05-16 |
Family
ID=20396889
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
ES96901592T Expired - Lifetime ES2186768T3 (es) | 1995-01-20 | 1996-01-18 | Procedimiento para la purificacion de acidos nucleicos cortos. |
Country Status (10)
Country | Link |
---|---|
US (1) | US5801237A (es) |
EP (1) | EP0804448B1 (es) |
JP (1) | JP4148991B2 (es) |
AT (1) | ATE225362T1 (es) |
AU (1) | AU686045B2 (es) |
CA (1) | CA2210855A1 (es) |
DE (1) | DE69624096T2 (es) |
ES (1) | ES2186768T3 (es) |
SE (1) | SE9500183D0 (es) |
WO (1) | WO1996022299A1 (es) |
Families Citing this family (19)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
EP0863910A1 (en) | 1995-10-19 | 1998-09-16 | NeXstar Pharmaceuticals, Inc. | Method for solution phase synthesis of oligonucleotides |
WO1999039195A1 (en) * | 1998-02-03 | 1999-08-05 | Arqule, Inc. | Rapid method for separation of small molecules using reverse phase high performance liquid chromatography |
US6969587B2 (en) * | 2000-04-04 | 2005-11-29 | Taylor Paul D | Detection of nucleic acid heteroduplex molecules by anion-exchange chromatography |
US7192560B2 (en) * | 2001-12-20 | 2007-03-20 | 3M Innovative Properties Company | Methods and devices for removal of organic molecules from biological mixtures using anion exchange |
US7347976B2 (en) * | 2001-12-20 | 2008-03-25 | 3M Innovative Properties Company | Methods and devices for removal of organic molecules from biological mixtures using a hydrophilic solid support in a hydrophobic matrix |
US20030153741A1 (en) * | 2002-02-08 | 2003-08-14 | Marek Kwiatkowski | Methods for separating oligonucleotides |
US7981600B2 (en) * | 2003-04-17 | 2011-07-19 | 3M Innovative Properties Company | Methods and devices for removal of organic molecules from biological mixtures using an anion exchange material that includes a polyoxyalkylene |
US20050130177A1 (en) | 2003-12-12 | 2005-06-16 | 3M Innovative Properties Company | Variable valve apparatus and methods |
US20050142570A1 (en) * | 2003-12-24 | 2005-06-30 | 3M Innovative Properties Company | Methods for nucleic acid isolation and kits using a microfluidic device and sedimenting reagent |
US7939249B2 (en) | 2003-12-24 | 2011-05-10 | 3M Innovative Properties Company | Methods for nucleic acid isolation and kits using a microfluidic device and concentration step |
US20050142571A1 (en) * | 2003-12-24 | 2005-06-30 | 3M Innovative Properties Company | Methods for nucleic acid isolation and kits using solid phase material |
US7727710B2 (en) | 2003-12-24 | 2010-06-01 | 3M Innovative Properties Company | Materials, methods, and kits for reducing nonspecific binding of molecules to a surface |
US7274852B1 (en) * | 2005-12-02 | 2007-09-25 | Adc Telecommunications, Inc. | Splice tray arrangement |
GB2445441B (en) * | 2006-09-26 | 2010-06-30 | Ge Healthcare Bio Sciences | Nucleic acid purification method |
GB2445442A (en) * | 2006-09-26 | 2008-07-09 | Ge Healthcare Bio Sciences | Nucleic acid purification using anion exchange |
GB2443505B (en) * | 2006-09-26 | 2008-12-31 | Ge Healthcare Bio Sciences | Nucleic acid purification method |
WO2009100172A1 (en) | 2008-02-07 | 2009-08-13 | Ge Healthcare Bio-Sciences Corp. | Isolation of dna, rna and protein from a single sample |
US11912983B2 (en) | 2019-12-05 | 2024-02-27 | Waters Technologies Corporation | Polyanionic acids to improve recovery and minimize system loss |
US20220017887A1 (en) * | 2020-07-14 | 2022-01-20 | Waters Technologies Corporation | Compositions, kits and methods useful for separating oligonucleotides from matrix components |
Family Cites Families (4)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
DE3433649A1 (de) * | 1984-09-13 | 1986-03-20 | Gesellschaft für Biotechnologische Forschung mbH (GBF), 3300 Braunschweig | Verfahren zur aufreinigung synthetischer oligonucleotide |
US5183885A (en) * | 1991-10-18 | 1993-02-02 | Applied Biosystems, Inc. | Method for chromatographic separation of synthetic phosphorothioate oligonucleotides |
EP0755400A1 (en) * | 1994-04-08 | 1997-01-29 | HYBRIDON, Inc. | Purification of oligodeoxynucleotide phosphorothioates using anion exchange chromatography |
CA2194350A1 (en) * | 1994-07-05 | 1996-01-18 | Patricia Puma | Purification of oligodeoxynucleotide phosphorothioates using deae 5pw anion ion-exchange chromatography and hydrophobic interaction chromatography |
-
1995
- 1995-01-20 SE SE9500183A patent/SE9500183D0/xx unknown
-
1996
- 1996-01-18 AT AT96901592T patent/ATE225362T1/de not_active IP Right Cessation
- 1996-01-18 US US08/860,860 patent/US5801237A/en not_active Expired - Lifetime
- 1996-01-18 DE DE69624096T patent/DE69624096T2/de not_active Expired - Lifetime
- 1996-01-18 ES ES96901592T patent/ES2186768T3/es not_active Expired - Lifetime
- 1996-01-18 EP EP96901592A patent/EP0804448B1/en not_active Expired - Lifetime
- 1996-01-18 JP JP52220896A patent/JP4148991B2/ja not_active Expired - Fee Related
- 1996-01-18 AU AU45929/96A patent/AU686045B2/en not_active Ceased
- 1996-01-18 WO PCT/SE1996/000043 patent/WO1996022299A1/en active IP Right Grant
- 1996-01-18 CA CA002210855A patent/CA2210855A1/en not_active Abandoned
Also Published As
Publication number | Publication date |
---|---|
JP4148991B2 (ja) | 2008-09-10 |
DE69624096D1 (de) | 2002-11-07 |
SE9500183D0 (sv) | 1995-01-20 |
EP0804448A1 (en) | 1997-11-05 |
AU4592996A (en) | 1996-08-07 |
AU686045B2 (en) | 1998-01-29 |
ATE225362T1 (de) | 2002-10-15 |
CA2210855A1 (en) | 1996-07-25 |
EP0804448B1 (en) | 2002-10-02 |
WO1996022299A1 (en) | 1996-07-25 |
JPH10512566A (ja) | 1998-12-02 |
DE69624096T2 (de) | 2003-05-28 |
US5801237A (en) | 1998-09-01 |
Similar Documents
Publication | Publication Date | Title |
---|---|---|
ES2186768T3 (es) | Procedimiento para la purificacion de acidos nucleicos cortos. | |
ATE242317T1 (de) | Verfahren zur reinigung von viren mit chromatographie | |
ATE262348T1 (de) | Mittel zur verabreichung von antigenen | |
DE69735364D1 (de) | Modulatoren für die regenerierung von gewebe | |
ATE197479T1 (de) | Verfahren zur vervielfältigung von nukleinsäuresequenzen | |
ATE301476T1 (de) | Methoden und zubehör zur immunisierung von wirtsorganismen durch verabreichung nackter polynucleotide , welche für antigene peptide codieren | |
DE68900109D1 (de) | Methode zur verbreitung von dueften in innenraeumen von kraftfahrzeugen. | |
IL98682A0 (en) | Random bio-oligomer library,a method of synthesis thereof,and a method of use thereof | |
FI970115A0 (fi) | Nukeliinihappoja sisältävä valmiste, sen valmistus ja käytöt | |
EP2267017A3 (en) | Binding polypeptides for B lymphocyte stimulator protein (BLyS) | |
ATE289200T1 (de) | Oligonucleotidzusammenstellung und verfahren zur regulierung der protein b7 expression | |
EP1679383A3 (en) | Method and device for purifying nucleid acids | |
DE69728880D1 (de) | KINASE FÜR DIE STELLEN-SPEZIFISCHE PHOSPHORYLIERUNG VON I-kappa-B-alpha | |
ATE232399T1 (de) | Verfahren zur modulierung des wachstums kollateraler arterien und / oder anderer arterien aus bestehenden arteriolen verbindungen | |
DE3462072D1 (en) | Iodothyronine immunoassays employing hms as tbp blocking agent | |
EP2011503A3 (en) | High affinity nucleic acid ligands of cytokines | |
DE69024806D1 (de) | Methode der Reinigung von Proteinen | |
ES2153090T3 (es) | Conjugado inmunoenzimatico, su procedimiento de preparacion y sus aplicaciones. | |
AU4317897A (en) | Pharmaceutical compositions comprising s-alkylisothiouronium derivatives | |
ATE194360T1 (de) | Verfahren und mittel für die herstellung von bindungsproteinen für inhibitoren von plasmaproteinasen | |
KR890004673A (ko) | 제균 성질을 갖는 유리 마이크로 비이드 및 그 제조방법. | |
CA2243578A1 (en) | High affinity nucleic acid ligands of complement system proteins | |
IT8447751A0 (it) | Composizione contenente polisaccaride per legare proteine specifiche, metodo per ottenerla e procedimento nonche' corredo di saggio che la impiegano | |
WO1998018942A3 (en) | Novel human rab proteins | |
DE59611353D1 (de) | Transketolase-verwandtes protein |