ES2182698B1 - Procedimiento para la extraccion cuantitativa de gluten en alimentos procesados o no procesados por calor, y composicion solubilizante de proteinas de gluten o un kit comercial que la contenga necesarios para su puesta en practica. - Google Patents
Procedimiento para la extraccion cuantitativa de gluten en alimentos procesados o no procesados por calor, y composicion solubilizante de proteinas de gluten o un kit comercial que la contenga necesarios para su puesta en practica.Info
- Publication number
- ES2182698B1 ES2182698B1 ES200101098A ES200101098A ES2182698B1 ES 2182698 B1 ES2182698 B1 ES 2182698B1 ES 200101098 A ES200101098 A ES 200101098A ES 200101098 A ES200101098 A ES 200101098A ES 2182698 B1 ES2182698 B1 ES 2182698B1
- Authority
- ES
- Spain
- Prior art keywords
- gluten
- processed
- heat
- putting
- practice
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Expired - Fee Related
Links
Classifications
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/02—Food
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/68—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving proteins, peptides or amino acids
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N2333/00—Assays involving biological materials from specific organisms or of a specific nature
- G01N2333/415—Assays involving biological materials from specific organisms or of a specific nature from plants
Landscapes
- Life Sciences & Earth Sciences (AREA)
- Health & Medical Sciences (AREA)
- Engineering & Computer Science (AREA)
- Chemical & Material Sciences (AREA)
- Immunology (AREA)
- Food Science & Technology (AREA)
- Pathology (AREA)
- Molecular Biology (AREA)
- Biochemistry (AREA)
- General Health & Medical Sciences (AREA)
- General Physics & Mathematics (AREA)
- Physics & Mathematics (AREA)
- Medicinal Chemistry (AREA)
- Urology & Nephrology (AREA)
- Biomedical Technology (AREA)
- Analytical Chemistry (AREA)
- Hematology (AREA)
- Cell Biology (AREA)
- Microbiology (AREA)
- Biotechnology (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Peptides Or Proteins (AREA)
- Preparation Of Compounds By Using Micro-Organisms (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
- Investigating Or Analysing Biological Materials (AREA)
Abstract
Procedimiento para la extracción de gluten en alimentos procesados y no procesados por calor, compatible con Elisa, composición y kits que comprenden dicha composición. El procedimiento comprende extraer el gluten contenido en dicha muestra con una solución acuosa de etanol en presencia de una composición que comprende un agente reductor de grupos disulfuro y un agente disociante, en un tampón de pH comprendido entre 7 y 8. El procedimiento permite extraer cuantitativamente el gluten contenido en una muestra de un alimento, tanto procesado por calor como no procesado por calor, como paso previo a la cuantificación por Elisa del gluten. De aplicación en el análisis de alimentos, en particular, de los alimentos destinados; a los enfermos celiacos.
Priority Applications (10)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
ES200101098A ES2182698B1 (es) | 2001-05-14 | 2001-05-14 | Procedimiento para la extraccion cuantitativa de gluten en alimentos procesados o no procesados por calor, y composicion solubilizante de proteinas de gluten o un kit comercial que la contenga necesarios para su puesta en practica. |
EP02730303A EP1424345B1 (en) | 2001-05-14 | 2002-05-03 | Method for extracting gluten contained in heat-processed and non-heat-processed foodstuffs, compatible with an enzyme-linked immunosorbent assay, composition and kits comprising said composition |
DE60237695T DE60237695D1 (de) | 2001-05-14 | 2002-05-03 | Verfahren zum extrahieren von in wärmebehandelten und nichtwärmebehandelten nahrungsmitteln enthaltenem gluten, das mit einem enzyme-linked immunosorbent assay kompatibel ist, zusammensetzung und kits, die die zusammensetzung enthalten |
PCT/ES2002/000208 WO2002092633A1 (es) | 2001-05-14 | 2002-05-03 | Procedimiento para la extracción de gluten en alimentos, procesados y no procesados por calor, compatible con elisa, composición y kits que comprenden dicha composición |
ES02730303T ES2353862T3 (es) | 2001-05-14 | 2002-05-03 | Procedimiento para la extracción de gluten contenido en alimentos, procesados y no procesados por calor, compatible con un ensayo de inmunoabsorción ligado a enzimas, composición y kits que comprenden dicha composición. |
DK02730303.1T DK1424345T3 (da) | 2001-05-14 | 2002-05-03 | Fremgangsmåde til at ekstrahere gluten indeholdt i varmebehandlede og ikke-varmebehandlede fødevarer, kompatibelt med et enzymforbundet immunosorbentassay, sammensætning og kits omfattende nævnte sammensætning |
US10/477,935 US7585529B2 (en) | 2001-05-14 | 2002-05-03 | Process to extract gluten from food products and kit for achieving same |
CA2447085A CA2447085C (en) | 2001-05-14 | 2002-05-03 | Process to extract gluten from heat-treated or non heat-treated food products, compatible with elisa, composition and kits that contain this composition |
AT02730303T ATE481423T1 (de) | 2001-05-14 | 2002-05-03 | Verfahren zum extrahieren von in wärmebehandelten und nichtwärmebehandelten nahrungsmitteln enthaltenem gluten, das mit einem enzyme-linked immunosorbent assay kompatibel ist, zusammensetzung und kits, die die zusammensetzung enthalten |
PT02730303T PT1424345E (pt) | 2001-05-14 | 2002-05-03 | Método de extracção do glúten contido em alimentos sujeitos a processamento térmico ou sem processamento térmico, compatível com um ensaio imunoabsorvente com ligação enzimática, composição e conjuntos que compreendem a referida composição |
Applications Claiming Priority (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
ES200101098A ES2182698B1 (es) | 2001-05-14 | 2001-05-14 | Procedimiento para la extraccion cuantitativa de gluten en alimentos procesados o no procesados por calor, y composicion solubilizante de proteinas de gluten o un kit comercial que la contenga necesarios para su puesta en practica. |
Publications (2)
Publication Number | Publication Date |
---|---|
ES2182698A1 ES2182698A1 (es) | 2003-03-01 |
ES2182698B1 true ES2182698B1 (es) | 2004-09-16 |
Family
ID=8497712
Family Applications (2)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
ES200101098A Expired - Fee Related ES2182698B1 (es) | 2001-05-14 | 2001-05-14 | Procedimiento para la extraccion cuantitativa de gluten en alimentos procesados o no procesados por calor, y composicion solubilizante de proteinas de gluten o un kit comercial que la contenga necesarios para su puesta en practica. |
ES02730303T Expired - Lifetime ES2353862T3 (es) | 2001-05-14 | 2002-05-03 | Procedimiento para la extracción de gluten contenido en alimentos, procesados y no procesados por calor, compatible con un ensayo de inmunoabsorción ligado a enzimas, composición y kits que comprenden dicha composición. |
Family Applications After (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
ES02730303T Expired - Lifetime ES2353862T3 (es) | 2001-05-14 | 2002-05-03 | Procedimiento para la extracción de gluten contenido en alimentos, procesados y no procesados por calor, compatible con un ensayo de inmunoabsorción ligado a enzimas, composición y kits que comprenden dicha composición. |
Country Status (9)
Country | Link |
---|---|
US (1) | US7585529B2 (es) |
EP (1) | EP1424345B1 (es) |
AT (1) | ATE481423T1 (es) |
CA (1) | CA2447085C (es) |
DE (1) | DE60237695D1 (es) |
DK (1) | DK1424345T3 (es) |
ES (2) | ES2182698B1 (es) |
PT (1) | PT1424345E (es) |
WO (1) | WO2002092633A1 (es) |
Families Citing this family (15)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
ES2217931B1 (es) * | 2002-07-01 | 2006-02-16 | Universidad De Barcelona | Metodo de diagnostico de la evolucion de la masa intestinal absortiva en un individuo. |
JP3600231B1 (ja) * | 2003-09-30 | 2004-12-15 | 森永製菓株式会社 | イムノアッセイ |
ES2239545B1 (es) * | 2004-03-15 | 2006-12-16 | Consejo Sup. Investig. Cientificas | Elisa competitivo para la deteccion de gluten hidrolizado y sus aplicaciones. |
US20100009391A1 (en) * | 2006-03-16 | 2010-01-14 | Consejo Superior De Investigaciones Cientificas | Method for extracting gluten from processed and unprocessed foods by means of heat based on the use of ionic and non-ionic detergents |
ES2349915B1 (es) | 2008-08-07 | 2011-11-15 | Universitat Rovira I Virgili | Procedimientos para la extraccion de prolaminas toxicas del gluten enceliaquia. |
ES2351021B1 (es) * | 2009-07-13 | 2011-12-27 | Consejo Superior De Investigaciones Científicas (Csic) | Método para la extracción de gluten nativo e hidrolizado. |
ES2392412B1 (es) * | 2010-11-03 | 2013-09-13 | Biomedal S L | Solución para extracción de gluten y métodos de uso. |
CN102809657B (zh) * | 2012-08-29 | 2015-07-29 | 沃克(天津)生物科技有限公司 | 食物不耐受血清特异性IgG检测试剂盒及其制备方法 |
US10254279B2 (en) | 2013-03-29 | 2019-04-09 | Nima Labs, Inc. | System and method for detection of target substances |
US10249035B2 (en) | 2013-03-29 | 2019-04-02 | Nima Labs, Inc. | System and method for detecting target substances |
EP2979092B1 (en) | 2013-03-29 | 2018-05-30 | Nima Labs, Inc. | A portable device for detection of harmful substances |
US10466236B2 (en) | 2013-03-29 | 2019-11-05 | Nima Labs, Inc. | System and method for detecting target substances |
EP3524983A1 (de) | 2018-02-08 | 2019-08-14 | R-Biopharm Aktiengesellschaft | Verfahren zur quantifizierung von gesamtgluten aus getreide in lebensmittelproben |
FR3104260A1 (fr) | 2019-12-04 | 2021-06-11 | Commissariat à l'Energie Atomique et aux Energies Alternatives | Procédé de détection d'une espèce allergène, contaminante ou de nature frauduleuse dans un échantillon agro-alimentaire |
FR3143127A1 (fr) | 2022-12-07 | 2024-06-14 | Commissariat à l'Energie Atomique et aux Energies Alternatives | Procédé de détection d'une espèce allergène, contaminante ou de nature frauduleuse dans un échantillon agro-alimentaire |
Family Cites Families (3)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US4658830A (en) * | 1984-08-08 | 1987-04-21 | Survival Technology, Inc. | Method and apparatus for initiating reperfusion treatment by an unattended individual undergoing heart attack symptoms |
GB2207921B (en) * | 1987-07-08 | 1991-05-29 | Commw Scient Ind Res Org | Monoclonal antibody to omega-gliadin and hybridoma thereof |
GB8823027D0 (en) * | 1988-09-30 | 1988-11-09 | Agricultural & Food Res | Determining cereal quality |
-
2001
- 2001-05-14 ES ES200101098A patent/ES2182698B1/es not_active Expired - Fee Related
-
2002
- 2002-05-03 ES ES02730303T patent/ES2353862T3/es not_active Expired - Lifetime
- 2002-05-03 AT AT02730303T patent/ATE481423T1/de active
- 2002-05-03 PT PT02730303T patent/PT1424345E/pt unknown
- 2002-05-03 DE DE60237695T patent/DE60237695D1/de not_active Expired - Lifetime
- 2002-05-03 DK DK02730303.1T patent/DK1424345T3/da active
- 2002-05-03 EP EP02730303A patent/EP1424345B1/en not_active Expired - Lifetime
- 2002-05-03 CA CA2447085A patent/CA2447085C/en not_active Expired - Lifetime
- 2002-05-03 US US10/477,935 patent/US7585529B2/en not_active Expired - Lifetime
- 2002-05-03 WO PCT/ES2002/000208 patent/WO2002092633A1/es not_active Application Discontinuation
Non-Patent Citations (2)
Title |
---|
ARIS GRAVELAND et al. Extraction and Fraction of Wheat Flour Proteins. J. Sc. Food Agric., 1982, Vol. 33, páginas 1117-1128. * |
PETER J. FRAZIER et al. Lipid-protein Interaction During Dough Development. J. Ser. Food Agric., 1981, Vol. 32, páginas 377-897. * |
Also Published As
Publication number | Publication date |
---|---|
CA2447085A1 (en) | 2002-11-21 |
ES2182698A1 (es) | 2003-03-01 |
EP1424345A1 (en) | 2004-06-02 |
DK1424345T3 (da) | 2011-01-24 |
WO2002092633A1 (es) | 2002-11-21 |
DE60237695D1 (de) | 2010-10-28 |
ES2353862T3 (es) | 2011-03-07 |
US20040137137A1 (en) | 2004-07-15 |
US7585529B2 (en) | 2009-09-08 |
PT1424345E (pt) | 2010-12-22 |
EP1424345B1 (en) | 2010-09-15 |
CA2447085C (en) | 2013-07-09 |
ATE481423T1 (de) | 2010-10-15 |
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Legal Events
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