ES2180453A1 - Metodo de deteccion y evaluacion de compuestos mimeticos de paclitaxel - Google Patents
Metodo de deteccion y evaluacion de compuestos mimeticos de paclitaxelInfo
- Publication number
- ES2180453A1 ES2180453A1 ES200101710A ES200101710A ES2180453A1 ES 2180453 A1 ES2180453 A1 ES 2180453A1 ES 200101710 A ES200101710 A ES 200101710A ES 200101710 A ES200101710 A ES 200101710A ES 2180453 A1 ES2180453 A1 ES 2180453A1
- Authority
- ES
- Spain
- Prior art keywords
- paclitaxel
- detecting
- microtubules
- analysing
- mimetic compounds
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Granted
Links
Classifications
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/58—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving labelled substances
- G01N33/582—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving labelled substances with fluorescent label
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
- G01N33/566—Immunoassay; Biospecific binding assay; Materials therefor using specific carrier or receptor proteins as ligand binding reagents where possible specific carrier or receptor proteins are classified with their target compounds
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/68—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving proteins, peptides or amino acids
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N2500/00—Screening for compounds of potential therapeutic value
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N2500/00—Screening for compounds of potential therapeutic value
- G01N2500/02—Screening involving studying the effect of compounds C on the interaction between interacting molecules A and B (e.g. A = enzyme and B = substrate for A, or A = receptor and B = ligand for the receptor)
Landscapes
- Life Sciences & Earth Sciences (AREA)
- Health & Medical Sciences (AREA)
- Engineering & Computer Science (AREA)
- Immunology (AREA)
- Molecular Biology (AREA)
- Biomedical Technology (AREA)
- Chemical & Material Sciences (AREA)
- Hematology (AREA)
- Urology & Nephrology (AREA)
- Food Science & Technology (AREA)
- General Physics & Mathematics (AREA)
- Cell Biology (AREA)
- Biotechnology (AREA)
- Medicinal Chemistry (AREA)
- Physics & Mathematics (AREA)
- Analytical Chemistry (AREA)
- Biochemistry (AREA)
- General Health & Medical Sciences (AREA)
- Microbiology (AREA)
- Pathology (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Epoxy Compounds (AREA)
- Investigating Or Analysing Biological Materials (AREA)
- Pharmaceuticals Containing Other Organic And Inorganic Compounds (AREA)
- Investigating, Analyzing Materials By Fluorescence Or Luminescence (AREA)
- Nitrogen And Oxygen Or Sulfur-Condensed Heterocyclic Ring Systems (AREA)
- Saccharide Compounds (AREA)
Abstract
Método de detección y evaluación de compuestos miméticos de paclitaxel. Una gran parte de los citostáticos, entre ellos paclitaxel (Taxol ®) y otras substancias recientemente descubiertas que mimetizan sus efectos antitumorales, tienen como diana los microtúbulos celulares. La presente invención es un ensayo homogéneo de alto rendimiento, basado en la utilización de microtúbulos estabilizados y taxoides fluorescentes, para detectar cualesquiera substancias que puedan sustituir al paclitaxel en su sitio de unión en los microtúbulos, y constituir potencialmente agentes anticancerosos.
Priority Applications (8)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
ES200101710A ES2180453B1 (es) | 2001-07-20 | 2001-07-20 | Metodo de deteccion y evaluacion de compuestos mimeticos de paclitaxel. |
ES02735435T ES2287280T3 (es) | 2001-07-20 | 2002-05-31 | Metodo de deteccion y evaluacion de compuestos mimeticos de placlitaxel. |
CA002454396A CA2454396A1 (en) | 2001-07-20 | 2002-05-31 | Method of detecting and analysing paclitaxel-mimetic compounds |
EP02735435A EP1420249B1 (en) | 2001-07-20 | 2002-05-31 | Method of detecting and analysing paclitaxel-mimetic compounds |
AT02735435T ATE363071T1 (de) | 2001-07-20 | 2002-05-31 | Verfahren zum nachweis und zur analyse von paclitaxel imitierenden verbindungen |
DE60220286T DE60220286D1 (de) | 2001-07-20 | 2002-05-31 | Verfahren zum nachweis und zur analyse von paclitaxel imitierenden verbindungen |
PCT/ES2002/000262 WO2003008965A1 (es) | 2001-07-20 | 2002-05-31 | Metodo de deteccion y evaluacion de compuestos mimeticos de paclitaxel |
US10/761,150 US7476511B2 (en) | 2001-07-20 | 2004-01-20 | Method of detecting and analyzing paclitaxel-mimetic compounds |
Applications Claiming Priority (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
ES200101710A ES2180453B1 (es) | 2001-07-20 | 2001-07-20 | Metodo de deteccion y evaluacion de compuestos mimeticos de paclitaxel. |
Publications (2)
Publication Number | Publication Date |
---|---|
ES2180453A1 true ES2180453A1 (es) | 2003-02-01 |
ES2180453B1 ES2180453B1 (es) | 2004-05-01 |
Family
ID=8498472
Family Applications (2)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
ES200101710A Expired - Fee Related ES2180453B1 (es) | 2001-07-20 | 2001-07-20 | Metodo de deteccion y evaluacion de compuestos mimeticos de paclitaxel. |
ES02735435T Expired - Lifetime ES2287280T3 (es) | 2001-07-20 | 2002-05-31 | Metodo de deteccion y evaluacion de compuestos mimeticos de placlitaxel. |
Family Applications After (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
ES02735435T Expired - Lifetime ES2287280T3 (es) | 2001-07-20 | 2002-05-31 | Metodo de deteccion y evaluacion de compuestos mimeticos de placlitaxel. |
Country Status (7)
Country | Link |
---|---|
US (1) | US7476511B2 (es) |
EP (1) | EP1420249B1 (es) |
AT (1) | ATE363071T1 (es) |
CA (1) | CA2454396A1 (es) |
DE (1) | DE60220286D1 (es) |
ES (2) | ES2180453B1 (es) |
WO (1) | WO2003008965A1 (es) |
Families Citing this family (1)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US10399964B2 (en) | 2017-03-30 | 2019-09-03 | The University Of Kansas | Coumarin-linked taxanes for detection and circumvention of cellular efflux |
Citations (5)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US5340724A (en) * | 1991-04-25 | 1994-08-23 | The Upjohn Company | Use of taxol-dependent cells to identify and isolate taxol-like compounds |
WO1994020134A1 (en) * | 1993-03-03 | 1994-09-15 | The Trustees Of Columbia University In The City Of New Yrok | An endogenous taxol-like substance in human serum, monoclonal antibodies directed thereto, and methods of assaying therefor |
WO1997019938A1 (es) * | 1995-11-29 | 1997-06-05 | Consejo Superior Investigaciones Cientificas | Derivados fluorescentes de paclitaxel y docetaxel con actividad antineoplasica, metodo para obtenerlos y sus aplicaciones |
WO1999053295A1 (en) * | 1998-04-14 | 1999-10-21 | The Regents Of The University Of California | Assays for the detection of microtubule depolymerization inhibitors |
WO2000056894A1 (en) * | 1999-03-23 | 2000-09-28 | Cytoclonal Pharmaceutics, Inc. | Fungal beta-tubulin genes |
-
2001
- 2001-07-20 ES ES200101710A patent/ES2180453B1/es not_active Expired - Fee Related
-
2002
- 2002-05-31 EP EP02735435A patent/EP1420249B1/en not_active Expired - Lifetime
- 2002-05-31 WO PCT/ES2002/000262 patent/WO2003008965A1/es active IP Right Grant
- 2002-05-31 DE DE60220286T patent/DE60220286D1/de not_active Expired - Lifetime
- 2002-05-31 AT AT02735435T patent/ATE363071T1/de not_active IP Right Cessation
- 2002-05-31 ES ES02735435T patent/ES2287280T3/es not_active Expired - Lifetime
- 2002-05-31 CA CA002454396A patent/CA2454396A1/en not_active Abandoned
-
2004
- 2004-01-20 US US10/761,150 patent/US7476511B2/en not_active Expired - Fee Related
Patent Citations (5)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US5340724A (en) * | 1991-04-25 | 1994-08-23 | The Upjohn Company | Use of taxol-dependent cells to identify and isolate taxol-like compounds |
WO1994020134A1 (en) * | 1993-03-03 | 1994-09-15 | The Trustees Of Columbia University In The City Of New Yrok | An endogenous taxol-like substance in human serum, monoclonal antibodies directed thereto, and methods of assaying therefor |
WO1997019938A1 (es) * | 1995-11-29 | 1997-06-05 | Consejo Superior Investigaciones Cientificas | Derivados fluorescentes de paclitaxel y docetaxel con actividad antineoplasica, metodo para obtenerlos y sus aplicaciones |
WO1999053295A1 (en) * | 1998-04-14 | 1999-10-21 | The Regents Of The University Of California | Assays for the detection of microtubule depolymerization inhibitors |
WO2000056894A1 (en) * | 1999-03-23 | 2000-09-28 | Cytoclonal Pharmaceutics, Inc. | Fungal beta-tubulin genes |
Non-Patent Citations (2)
Title |
---|
DIAZ, J.F. y col. Molecular recognition of Taxol by microtubules: kinetics and thermodynamics of binding of fluorescent taxol derivatives to an exposed site. The Journal of the Biological Chemistry, 25 Agosto 2000, Vol. 275, Nö 34, páginas 26265-26276, ISSN 0021-9258. * |
NEUMANN, T. y col. Determination of the net exchange rate of tubulin dimer in steady-state microtubules by fluorescence correlation spectroscopy. Biological Chemistry, Marzo 2001, Vol. 382, Nö 3, páginas 387-391, ISSN 1431-6730. * |
Also Published As
Publication number | Publication date |
---|---|
ES2180453B1 (es) | 2004-05-01 |
ATE363071T1 (de) | 2007-06-15 |
US20040203082A1 (en) | 2004-10-14 |
WO2003008965A1 (es) | 2003-01-30 |
EP1420249A1 (en) | 2004-05-19 |
EP1420249B1 (en) | 2007-05-23 |
DE60220286D1 (de) | 2007-07-05 |
ES2287280T3 (es) | 2007-12-16 |
US7476511B2 (en) | 2009-01-13 |
CA2454396A1 (en) | 2003-01-30 |
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Legal Events
Date | Code | Title | Description |
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EC2A | Search report published |
Date of ref document: 20030201 Kind code of ref document: A1 |
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