ES2173670T3 - Procedimiento de identificacion de acidos nucleicos mediante espectrometria de masas laser de desorcion/ionizacion asistidas por matriz. - Google Patents

Procedimiento de identificacion de acidos nucleicos mediante espectrometria de masas laser de desorcion/ionizacion asistidas por matriz.

Info

Publication number
ES2173670T3
ES2173670T3 ES98966608T ES98966608T ES2173670T3 ES 2173670 T3 ES2173670 T3 ES 2173670T3 ES 98966608 T ES98966608 T ES 98966608T ES 98966608 T ES98966608 T ES 98966608T ES 2173670 T3 ES2173670 T3 ES 2173670T3
Authority
ES
Spain
Prior art keywords
matrix
nucleic acids
desortion
mass spectrometry
identification procedure
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired - Lifetime
Application number
ES98966608T
Other languages
English (en)
Inventor
Ivo Glynne Gut
Kurt Berlin
Hans Lehrach
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Max Planck Gesellschaft zur Foerderung der Wissenschaften eV
Original Assignee
Max Planck Gesellschaft zur Foerderung der Wissenschaften eV
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Max Planck Gesellschaft zur Foerderung der Wissenschaften eV filed Critical Max Planck Gesellschaft zur Foerderung der Wissenschaften eV
Application granted granted Critical
Publication of ES2173670T3 publication Critical patent/ES2173670T3/es
Anticipated expiration legal-status Critical
Expired - Lifetime legal-status Critical Current

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Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6869—Methods for sequencing
    • C12Q1/6872—Methods for sequencing involving mass spectrometry
    • H—ELECTRICITY
    • H01—ELECTRIC ELEMENTS
    • H01J—ELECTRIC DISCHARGE TUBES OR DISCHARGE LAMPS
    • H01J49/00—Particle spectrometers or separator tubes
    • H01J49/02—Details
    • H01J49/04—Arrangements for introducing or extracting samples to be analysed, e.g. vacuum locks; Arrangements for external adjustment of electron- or ion-optical components
    • H01J49/0409—Sample holders or containers
    • H01J49/0418—Sample holders or containers for laser desorption, e.g. matrix-assisted laser desorption/ionisation [MALDI] plates or surface enhanced laser desorption/ionisation [SELDI] plates

Landscapes

  • Life Sciences & Earth Sciences (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Physics & Mathematics (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Molecular Biology (AREA)
  • Biochemistry (AREA)
  • Microbiology (AREA)
  • Biotechnology (AREA)
  • Biophysics (AREA)
  • Analytical Chemistry (AREA)
  • Spectroscopy & Molecular Physics (AREA)
  • Immunology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Other Investigation Or Analysis Of Materials By Electrical Means (AREA)
  • Investigating Or Analysing Biological Materials (AREA)

Abstract

Procedimiento para identificar una secuencia de nucleótidos en una molécula de ácido nucleico, el cual comprende las siguientes etapas: (a) hibridación de moléculas de ácido nucleico con una serie de sondas de distintas secuencias de núcleobases, en que cada sonda posee una masa diferente de todas las demás; (b) separación de las sondas no hibridadas; (c) puesta en contacto de las sondas hibridadas con una matriz que favorece la desorción de las sondas mediante un rayo láser; (d) análisis de las sondas hibridadas y envueltas por la matriz, sobre un soporte de muestras formado por material eléctricamente conductor, en un espectrómetro de masas, y (e) determinación de las moléculas de ácido nucleico que presentan la secuencia, de modo que las posiciones de las sondas en el soporte de muestras permiten una asignacióna la molécula de ácido nucleico con la cual se hibridan.
ES98966608T 1997-12-05 1998-12-04 Procedimiento de identificacion de acidos nucleicos mediante espectrometria de masas laser de desorcion/ionizacion asistidas por matriz. Expired - Lifetime ES2173670T3 (es)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
EP97121471 1997-12-05

Publications (1)

Publication Number Publication Date
ES2173670T3 true ES2173670T3 (es) 2002-10-16

Family

ID=8227757

Family Applications (1)

Application Number Title Priority Date Filing Date
ES98966608T Expired - Lifetime ES2173670T3 (es) 1997-12-05 1998-12-04 Procedimiento de identificacion de acidos nucleicos mediante espectrometria de masas laser de desorcion/ionizacion asistidas por matriz.

Country Status (8)

Country Link
EP (1) EP1036202B1 (es)
JP (1) JP2001526381A (es)
AT (1) ATE217028T1 (es)
CA (1) CA2312052A1 (es)
DE (1) DE59804008D1 (es)
DK (1) DK1036202T3 (es)
ES (1) ES2173670T3 (es)
WO (1) WO1999029898A2 (es)

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DE19957827C2 (de) * 1999-11-25 2003-06-12 Epigenomics Ag Verwendung eines Oligomer-Arrays mit PNA- und/oder DNA-Oligomeren auf einer Oberfläche
AU2001278420A1 (en) * 2000-04-06 2001-11-07 Epigenomics Ag Diagnosis of diseases associated with dna repair
DE10027120A1 (de) 2000-05-23 2001-12-06 Epigenomics Ag Probenträger für Massenspektrometer
JP2004501666A (ja) * 2000-06-30 2004-01-22 エピゲノミクス　アーゲー 薬理ゲノミクスのメチル化状態分析のための方法及び核酸
DE10054974A1 (de) * 2000-11-06 2002-06-06 Epigenomics Ag Diagnose von mit Cdk4 assoziierten Krankheiten
DE10061338A1 (de) * 2000-12-06 2002-06-20 Epigenomics Ag Diagnose von mit Angiogenese assoziierten Krankheiten
EP1215199A1 (en) * 2000-12-08 2002-06-19 Sony International (Europe) GmbH Linker molecules for selective metallisation of nucleic acids and their uses
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JP2002372517A (ja) * 2001-05-31 2002-12-26 Inst Of Physical & Chemical Res 質量分析法によるタンパク質の構造解析方法
US8073627B2 (en) 2001-06-26 2011-12-06 Ibis Biosciences, Inc. System for indentification of pathogens
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JP2006516193A (ja) 2002-12-06 2006-06-29 アイシス・ファーマシューティカルス・インコーポレーテッド ヒトおよび動物における病原体の迅速な同定方法
US8158354B2 (en) 2003-05-13 2012-04-17 Ibis Biosciences, Inc. Methods for rapid purification of nucleic acids for subsequent analysis by mass spectrometry by solution capture
US7964343B2 (en) 2003-05-13 2011-06-21 Ibis Biosciences, Inc. Method for rapid purification of nucleic acids for subsequent analysis by mass spectrometry by solution capture
US8546082B2 (en) 2003-09-11 2013-10-01 Ibis Biosciences, Inc. Methods for identification of sepsis-causing bacteria
US8394945B2 (en) 2003-09-11 2013-03-12 Ibis Biosciences, Inc. Compositions for use in identification of bacteria
US8097416B2 (en) 2003-09-11 2012-01-17 Ibis Biosciences, Inc. Methods for identification of sepsis-causing bacteria
US7666592B2 (en) 2004-02-18 2010-02-23 Ibis Biosciences, Inc. Methods for concurrent identification and quantification of an unknown bioagent
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US20050266411A1 (en) 2004-05-25 2005-12-01 Hofstadler Steven A Methods for rapid forensic analysis of mitochondrial DNA
US7811753B2 (en) 2004-07-14 2010-10-12 Ibis Biosciences, Inc. Methods for repairing degraded DNA
WO2006135400A2 (en) 2004-08-24 2006-12-21 Isis Pharmaceuticals, Inc. Methods for rapid identification of recombinant organisms
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US8084207B2 (en) 2005-03-03 2011-12-27 Ibis Bioscience, Inc. Compositions for use in identification of papillomavirus
WO2007014045A2 (en) 2005-07-21 2007-02-01 Isis Pharmaceuticals, Inc. Methods for rapid identification and quantitation of nucleic acid variants
EP1775348A1 (en) * 2005-10-17 2007-04-18 Max-Planck-Gesellschaft zur Förderung der Wissenschaften e.V. Method of analyzing nucleic acid with mass spectrometry
WO2007118222A2 (en) 2006-04-06 2007-10-18 Ibis Biosciences, INC Compositions for the use in identification of fungi
CA2663029C (en) 2006-09-14 2016-07-19 Ibis Biosciences, Inc. Targeted whole genome amplification method for identification of pathogens
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JP5680304B2 (ja) 2007-02-23 2015-03-04 アイビス バイオサイエンシズ インコーポレイティッド 迅速な法医学的ｄｎａ分析法
US9598724B2 (en) 2007-06-01 2017-03-21 Ibis Biosciences, Inc. Methods and compositions for multiple displacement amplification of nucleic acids
US8534447B2 (en) 2008-09-16 2013-09-17 Ibis Biosciences, Inc. Microplate handling systems and related computer program products and methods
WO2010033599A2 (en) 2008-09-16 2010-03-25 Ibis Biosciences, Inc. Mixing cartridges, mixing stations, and related kits, systems, and methods
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Also Published As

Publication number Publication date
JP2001526381A (ja) 2001-12-18
WO1999029898A2 (de) 1999-06-17
CA2312052A1 (en) 1999-06-17
WO1999029898A3 (de) 1999-10-28
DK1036202T3 (da) 2002-08-12
EP1036202B1 (de) 2002-05-02
ATE217028T1 (de) 2002-05-15
DE59804008D1 (de) 2002-06-06
EP1036202A2 (de) 2000-09-20

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