ES2169943T3 - Metodo para la amplificacion no especifica de acido nucleico. - Google Patents
Metodo para la amplificacion no especifica de acido nucleico.Info
- Publication number
- ES2169943T3 ES2169943T3 ES99904801T ES99904801T ES2169943T3 ES 2169943 T3 ES2169943 T3 ES 2169943T3 ES 99904801 T ES99904801 T ES 99904801T ES 99904801 T ES99904801 T ES 99904801T ES 2169943 T3 ES2169943 T3 ES 2169943T3
- Authority
- ES
- Spain
- Prior art keywords
- sequence
- enzyme
- activity
- nucleic acid
- oligo
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Expired - Lifetime
Links
- 238000000034 method Methods 0.000 title abstract 3
- 108020004707 nucleic acids Proteins 0.000 title abstract 2
- 150000007523 nucleic acids Chemical class 0.000 title abstract 2
- 102000039446 nucleic acids Human genes 0.000 title abstract 2
- 230000003321 amplification Effects 0.000 title 1
- 238000003199 nucleic acid amplification method Methods 0.000 title 1
- 102000004190 Enzymes Human genes 0.000 abstract 3
- 108090000790 Enzymes Proteins 0.000 abstract 3
- 230000000694 effects Effects 0.000 abstract 3
- 102000004163 DNA-directed RNA polymerases Human genes 0.000 abstract 2
- 108090000626 DNA-directed RNA polymerases Proteins 0.000 abstract 2
- 102100034343 Integrase Human genes 0.000 abstract 2
- 108091034117 Oligonucleotide Proteins 0.000 abstract 2
- 239000000463 material Substances 0.000 abstract 2
- 108020004999 messenger RNA Proteins 0.000 abstract 2
- 108091032973 (ribonucleotides)n+m Proteins 0.000 abstract 1
- 101710203526 Integrase Proteins 0.000 abstract 1
- 108010092799 RNA-directed DNA polymerase Proteins 0.000 abstract 1
- 108091028733 RNTP Proteins 0.000 abstract 1
- 230000002255 enzymatic effect Effects 0.000 abstract 1
- 239000011541 reaction mixture Substances 0.000 abstract 1
- 230000005026 transcription initiation Effects 0.000 abstract 1
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6844—Nucleic acid amplification reactions
- C12Q1/6865—Promoter-based amplification, e.g. nucleic acid sequence amplification [NASBA], self-sustained sequence replication [3SR] or transcription-based amplification system [TAS]
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
- C12N15/1096—Processes for the isolation, preparation or purification of DNA or RNA cDNA Synthesis; Subtracted cDNA library construction, e.g. RT, RT-PCR
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6844—Nucleic acid amplification reactions
- C12Q1/6853—Nucleic acid amplification reactions using modified primers or templates
Landscapes
- Chemical & Material Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Organic Chemistry (AREA)
- Health & Medical Sciences (AREA)
- Engineering & Computer Science (AREA)
- Zoology (AREA)
- Wood Science & Technology (AREA)
- Genetics & Genomics (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Biotechnology (AREA)
- General Engineering & Computer Science (AREA)
- Biochemistry (AREA)
- Biophysics (AREA)
- Microbiology (AREA)
- Physics & Mathematics (AREA)
- Molecular Biology (AREA)
- General Health & Medical Sciences (AREA)
- Analytical Chemistry (AREA)
- Immunology (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Biomedical Technology (AREA)
- Plant Pathology (AREA)
- Crystallography & Structural Chemistry (AREA)
- Bioinformatics & Computational Biology (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
- Saccharide Compounds (AREA)
- Preparation Of Compounds By Using Micro-Organisms (AREA)
- Steroid Compounds (AREA)
Abstract
Método para crear, de manera no específica, múltiples copias de RNA partiendo de ácido nucleico que contiene material inicial que comprende un conjunto de mRNAs, comprendiendo cada mRNA un terminal de poli-A, en donde el material se pone simultáneamente en contacto con: S un oligonucleótido que comprende una secuencia oligo- dT en su extremo 3'', la secuencia de un promotor reconocido por una RNA polimerasa en su extremo 5'' y una región de iniciación de transcripción que se localiza entre la secuencia oligo-dT y la secuencia del promotor, en donde el oligonucleótido se bloquea en su extremo 3'', de tal manera que se prohíbe la extensión a partir de ahí, S un enzima que tiene actividad de transcriptasa inversa, S un enzima que tiene actividad de RNasa H, y S un enzima que tiene actividad de RNA polimerasa, S suficiente cantidades de dNTPs y rNTPs, y la mezcla de reacción resultante se mantiene en las condiciones apropiadas durante un periodo de tiempo suficiente para que tengan lugar los procesos enzimáticos.
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| EP98200619 | 1998-02-27 |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| ES2169943T3 true ES2169943T3 (es) | 2002-07-16 |
Family
ID=8233425
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| ES99904801T Expired - Lifetime ES2169943T3 (es) | 1998-02-27 | 1999-01-21 | Metodo para la amplificacion no especifica de acido nucleico. |
Country Status (14)
| Country | Link |
|---|---|
| US (3) | US6338954B1 (es) |
| EP (2) | EP1170381A3 (es) |
| JP (1) | JP3641206B2 (es) |
| KR (1) | KR100440044B1 (es) |
| AT (1) | ATE210736T1 (es) |
| AU (1) | AU748847B2 (es) |
| CA (1) | CA2322073C (es) |
| DE (1) | DE69900592T2 (es) |
| DK (1) | DK1056884T3 (es) |
| ES (1) | ES2169943T3 (es) |
| ID (1) | ID26107A (es) |
| PT (1) | PT1056884E (es) |
| WO (1) | WO1999043850A1 (es) |
| ZA (1) | ZA99617B (es) |
Families Citing this family (25)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US6794138B1 (en) * | 1999-12-16 | 2004-09-21 | Affymetrix, Inc. | Methods of small sample amplification |
| US6582938B1 (en) * | 2001-05-11 | 2003-06-24 | Affymetrix, Inc. | Amplification of nucleic acids |
| WO2001064959A1 (en) * | 2000-03-02 | 2001-09-07 | Akzo Nobel N.V. | Detection of hepatitis b virus rna |
| US9261460B2 (en) * | 2002-03-12 | 2016-02-16 | Enzo Life Sciences, Inc. | Real-time nucleic acid detection processes and compositions |
| US7727722B2 (en) | 2001-08-29 | 2010-06-01 | General Electric Company | Ligation amplification |
| US7166478B2 (en) * | 2002-03-12 | 2007-01-23 | Enzo Life Sciences, Inc., C/O Enzo Biochem, Inc. | Labeling reagents and labeled targets, target labeling processes and other processes for using same in nucleic acid determinations and analyses |
| US9353405B2 (en) | 2002-03-12 | 2016-05-31 | Enzo Life Sciences, Inc. | Optimized real time nucleic acid detection processes |
| WO2004044239A1 (en) * | 2002-10-30 | 2004-05-27 | Pamgene B.V. | Improved methods for generating multiple rna copies |
| US9487823B2 (en) | 2002-12-20 | 2016-11-08 | Qiagen Gmbh | Nucleic acid amplification |
| US6852494B2 (en) | 2003-01-10 | 2005-02-08 | Linden Technologies, Inc. | Nucleic acid amplification |
| AU2004239283B2 (en) * | 2003-05-09 | 2010-01-28 | Genisphere, Llc | Methods for amplification of nucleic acid sequences using staggered ligation |
| US20050153333A1 (en) * | 2003-12-02 | 2005-07-14 | Sooknanan Roy R. | Selective terminal tagging of nucleic acids |
| DE602005017252D1 (de) | 2004-04-01 | 2009-12-03 | Genisphere Inc | Verfahren zur amplifikation von nukleinsäuren mittels promotor-templates |
| FR2876116B1 (fr) * | 2004-10-01 | 2007-04-06 | Biomerieux Sa | Methode pour generer des transcrits |
| US20100173279A1 (en) * | 2004-11-15 | 2010-07-08 | Mimi Healy | Detection using primers to repetitive dna and transcription-based amplification thereby |
| ATE464381T1 (de) | 2004-12-23 | 2010-04-15 | Ge Healthcare Bio Sciences | Rna-amplifikation auf ligationsbasis |
| AU2012200749B2 (en) * | 2004-12-23 | 2012-10-04 | Global Life Sciences Solutions Usa Llc | Ligation-based RNA amplification |
| EP1863908B1 (de) | 2005-04-01 | 2010-11-17 | Qiagen GmbH | Reverse transkription und amplifikation von rna bei simultaner degradierung von dna |
| EP1762627A1 (de) | 2005-09-09 | 2007-03-14 | Qiagen GmbH | Verfahren zur Aktivierung einer Nukleinsäure für eine Polymerase-Reaktion |
| DE102006038113A1 (de) * | 2006-08-14 | 2008-02-21 | Qiagen Gmbh | Verfahren zur Synthese einer cDNA in einer Probe in einer enzymatischen Reaktion und gleichzeitigen Bereitstellung eines ersten Enzyms mit Polyadenylierungsaktivität und eines zweiten Enzyms mit reverser Transkriptaseaktivität |
| JP5409631B2 (ja) * | 2007-08-13 | 2014-02-05 | キアゲン ゲゼルシャフト ミット ベシュレンクテル ハフツング | 酵素反応においてサンプル中でｃＤＮＡを合成するための方法 |
| US8951731B2 (en) * | 2007-10-15 | 2015-02-10 | Complete Genomics, Inc. | Sequence analysis using decorated nucleic acids |
| US8703421B2 (en) | 2008-05-30 | 2014-04-22 | Gen-Probe Incorporated | Compositions, kits and related methods for the detection and/or monitoring of Salmonella |
| EP2280080A1 (en) | 2009-07-31 | 2011-02-02 | Qiagen GmbH | Method of normalized quantification of nucleic acids using anchor oligonucleotides and adapter oligonucleotides |
| EP2280081A1 (en) * | 2009-07-31 | 2011-02-02 | Qiagen GmbH | Method of normalized quantification of RNA |
Family Cites Families (12)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US4661450A (en) * | 1983-05-03 | 1987-04-28 | Molecular Genetics Research And Development Limited Partnership | Molecular cloning of RNA using RNA ligase and synthetic oligonucleotides |
| US5472850A (en) * | 1991-04-10 | 1995-12-05 | Oklahoma Medical Research Foundation | Quantitative clotting assay for activated factor VII |
| US5480784A (en) | 1989-07-11 | 1996-01-02 | Gen-Probe Incorporated | Nucleic acid sequence amplification methods |
| US5166057A (en) | 1989-08-28 | 1992-11-24 | The Mount Sinai School Of Medicine Of The City University Of New York | Recombinant negative strand rna virus expression-systems |
| US5545522A (en) * | 1989-09-22 | 1996-08-13 | Van Gelder; Russell N. | Process for amplifying a target polynucleotide sequence using a single primer-promoter complex |
| US5169766A (en) | 1991-06-14 | 1992-12-08 | Life Technologies, Inc. | Amplification of nucleic acid molecules |
| AU681082B2 (en) * | 1992-05-06 | 1997-08-21 | Gen-Probe Incorporated | Nucleic acid sequence amplification method, composition and kit |
| US5514545A (en) * | 1992-06-11 | 1996-05-07 | Trustees Of The University Of Pennsylvania | Method for characterizing single cells based on RNA amplification for diagnostics and therapeutics |
| KR100392551B1 (ko) | 1994-07-15 | 2003-10-30 | 아크조 노벨 엔브이 | 핵산 증폭방법을 개선시키기 위한 rna폴리머라제의 용도 |
| FR2724934B1 (fr) * | 1994-09-26 | 1997-01-24 | Bio Merieux | Oligonucleotide chimere et son utilisation dans l'obtention de transcrits d'un acide nucleique |
| JP2002515738A (ja) * | 1996-01-23 | 2002-05-28 | アフィメトリックス，インコーポレイティド | 核酸分析法 |
| US6114152A (en) * | 1997-12-12 | 2000-09-05 | The Regents Of The University Of California | Methods for making nucleic acids |
-
1999
- 1999-01-21 DE DE69900592T patent/DE69900592T2/de not_active Expired - Fee Related
- 1999-01-21 WO PCT/EP1999/000332 patent/WO1999043850A1/en active IP Right Grant
- 1999-01-21 DK DK99904801T patent/DK1056884T3/da active
- 1999-01-21 KR KR10-2000-7009493A patent/KR100440044B1/ko not_active IP Right Cessation
- 1999-01-21 ID IDW20001643A patent/ID26107A/id unknown
- 1999-01-21 EP EP01121247A patent/EP1170381A3/en not_active Withdrawn
- 1999-01-21 JP JP2000533589A patent/JP3641206B2/ja not_active Expired - Fee Related
- 1999-01-21 AT AT99904801T patent/ATE210736T1/de not_active IP Right Cessation
- 1999-01-21 EP EP99904801A patent/EP1056884B9/en not_active Expired - Lifetime
- 1999-01-21 PT PT99904801T patent/PT1056884E/pt unknown
- 1999-01-21 AU AU25189/99A patent/AU748847B2/en not_active Ceased
- 1999-01-21 US US09/623,054 patent/US6338954B1/en not_active Expired - Fee Related
- 1999-01-21 CA CA002322073A patent/CA2322073C/en not_active Expired - Fee Related
- 1999-01-21 ES ES99904801T patent/ES2169943T3/es not_active Expired - Lifetime
- 1999-01-27 ZA ZA9900617A patent/ZA99617B/xx unknown
-
2001
- 2001-11-01 US US09/985,024 patent/US20020061533A1/en not_active Abandoned
-
2002
- 2002-12-11 US US10/316,456 patent/US20030113875A1/en not_active Abandoned
Also Published As
| Publication number | Publication date |
|---|---|
| KR20010041371A (ko) | 2001-05-15 |
| WO1999043850A1 (en) | 1999-09-02 |
| DE69900592T2 (de) | 2002-08-22 |
| ATE210736T1 (de) | 2001-12-15 |
| PT1056884E (pt) | 2002-06-28 |
| DE69900592D1 (de) | 2002-01-24 |
| US6338954B1 (en) | 2002-01-15 |
| US20020061533A1 (en) | 2002-05-23 |
| EP1056884B9 (en) | 2002-07-24 |
| CA2322073A1 (en) | 1999-09-02 |
| ID26107A (id) | 2000-11-23 |
| CA2322073C (en) | 2003-12-23 |
| JP2002505087A (ja) | 2002-02-19 |
| EP1056884B1 (en) | 2001-12-12 |
| JP3641206B2 (ja) | 2005-04-20 |
| EP1056884A1 (en) | 2000-12-06 |
| EP1170381A3 (en) | 2004-01-28 |
| ZA99617B (en) | 1999-07-27 |
| EP1170381A2 (en) | 2002-01-09 |
| AU748847B2 (en) | 2002-06-13 |
| AU2518999A (en) | 1999-09-15 |
| US20030113875A1 (en) | 2003-06-19 |
| KR100440044B1 (ko) | 2004-07-14 |
| DK1056884T3 (da) | 2002-02-25 |
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Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| FG2A | Definitive protection |
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