ES2158895T3 - Metodo para detectar un acido nucleico diana. - Google Patents
Metodo para detectar un acido nucleico diana.Info
- Publication number
- ES2158895T3 ES2158895T3 ES94916811T ES94916811T ES2158895T3 ES 2158895 T3 ES2158895 T3 ES 2158895T3 ES 94916811 T ES94916811 T ES 94916811T ES 94916811 T ES94916811 T ES 94916811T ES 2158895 T3 ES2158895 T3 ES 2158895T3
- Authority
- ES
- Spain
- Prior art keywords
- nucleic acid
- primer
- detecting
- target nucleic
- priming sequence
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Expired - Lifetime
Links
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6813—Hybridisation assays
- C12Q1/6816—Hybridisation assays characterised by the detection means
- C12Q1/6818—Hybridisation assays characterised by the detection means involving interaction of two or more labels, e.g. resonant energy transfer
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6844—Nucleic acid amplification reactions
- C12Q1/6848—Nucleic acid amplification reactions characterised by the means for preventing contamination or increasing the specificity or sensitivity of an amplification reaction
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6844—Nucleic acid amplification reactions
- C12Q1/6853—Nucleic acid amplification reactions using modified primers or templates
Landscapes
- Chemical & Material Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Organic Chemistry (AREA)
- Engineering & Computer Science (AREA)
- Zoology (AREA)
- Wood Science & Technology (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Health & Medical Sciences (AREA)
- Biophysics (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Immunology (AREA)
- Microbiology (AREA)
- Molecular Biology (AREA)
- Analytical Chemistry (AREA)
- Physics & Mathematics (AREA)
- Biotechnology (AREA)
- Biochemistry (AREA)
- Bioinformatics & Cheminformatics (AREA)
- General Engineering & Computer Science (AREA)
- General Health & Medical Sciences (AREA)
- Genetics & Genomics (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
- Saccharide Compounds (AREA)
Abstract
UN METODO PARA DETECTAR UN ACIDO NUCLEICO DIANA, EL CUAL INCLUYE LOS PASOS DE (I) AMPLIFICAR EL ACIDO NUCLEICO DIANA PARA OBTENER UN PRODUCTO DE AMPLIFICACION MEDIANTE UNA POLIMERASA, UN PRIMER INICIADOR CON O SIN UN SEGMENTO NO CONTIGUO A UNA PRIMERA SECUENCIA DE INICIO, Y UN SEGUNDO INICIADOR CON O SIN UN SEGMENTO NO CONTIGUO A UNA SEGUNDA SECUENCIA DE INICIO EN PRESENCIA DE UN OLIGONUCLEOTIDO QUE ES INCAPAZ DE ACTUAR COMO UN INICIADOR PARA LA POLIMERASA, EN DONDE EL OLIGONUCLEOTIDO TIENE AL MENOS 5 NUCLEOTIDOS CONSECUTIVOS COMPLETAMENTE COMPLEMENTARIOS A AL MENOS 5 NUCLEOTIDOS CONSECUTIVOS DEL PRIMER INICIADOR, Y (II) DETECTAR LA PRESENCIA DEL ACIDO NUCLEICO DIANA MEDIANTE LA SUPERVISION DE LA AMPLIFICACION DEL MISMO. EN CONCRETO, LA FIGURA ILUSTRA EL METODO EN DONDE EL INICIADOR CONTIENE UN SEGMENTO NO CONTIGUO A SU SECUENCIA DE INICIO.
Applications Claiming Priority (2)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
BR9408578A BR9408578A (pt) | 1994-05-23 | 1994-05-23 | Método para detecção de um ácido nucléico alvo |
PCT/US1994/005767 WO1995032306A1 (en) | 1994-05-23 | 1994-05-23 | Method for detecting a target nucleic acid |
Publications (1)
Publication Number | Publication Date |
---|---|
ES2158895T3 true ES2158895T3 (es) | 2001-09-16 |
Family
ID=40549889
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
ES94916811T Expired - Lifetime ES2158895T3 (es) | 1994-05-23 | 1994-05-23 | Metodo para detectar un acido nucleico diana. |
Country Status (13)
Country | Link |
---|---|
EP (1) | EP0763133B1 (es) |
JP (1) | JPH10500572A (es) |
AT (1) | ATE203775T1 (es) |
AU (1) | AU706033B2 (es) |
BR (1) | BR9408578A (es) |
DE (1) | DE69427876T2 (es) |
DK (1) | DK0763133T3 (es) |
ES (1) | ES2158895T3 (es) |
GR (1) | GR3037030T3 (es) |
HK (1) | HK1001131A1 (es) |
NZ (1) | NZ266724A (es) |
RU (1) | RU2158765C2 (es) |
WO (1) | WO1995032306A1 (es) |
Families Citing this family (16)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US5935522A (en) * | 1990-06-04 | 1999-08-10 | University Of Utah Research Foundation | On-line DNA analysis system with rapid thermal cycling |
US7081226B1 (en) | 1996-06-04 | 2006-07-25 | University Of Utah Research Foundation | System and method for fluorescence monitoring |
US7273749B1 (en) | 1990-06-04 | 2007-09-25 | University Of Utah Research Foundation | Container for carrying out and monitoring biological processes |
US5547861A (en) | 1994-04-18 | 1996-08-20 | Becton, Dickinson And Company | Detection of nucleic acid amplification |
US6174670B1 (en) | 1996-06-04 | 2001-01-16 | University Of Utah Research Foundation | Monitoring amplification of DNA during PCR |
ES2304573T3 (es) * | 1996-06-04 | 2008-10-16 | University Of Utah Research Foundation | Recipiente para llevar a cabo y controlar procesos biologicos. |
US6117635A (en) * | 1996-07-16 | 2000-09-12 | Intergen Company | Nucleic acid amplification oligonucleotides with molecular energy transfer labels and methods based thereon |
US5866336A (en) * | 1996-07-16 | 1999-02-02 | Oncor, Inc. | Nucleic acid amplification oligonucleotides with molecular energy transfer labels and methods based thereon |
JP4185185B2 (ja) * | 1997-05-08 | 2008-11-26 | 征夫 軽部 | 部分二重鎖dnaを利用したdnaの検出方法 |
WO1999042615A1 (en) * | 1998-02-18 | 1999-08-26 | Dade Behring Inc. | Methods for determining amounts of nucleic acids |
EP1709204A1 (en) * | 2004-01-23 | 2006-10-11 | Lingvitae AS | Improving polynucleotide ligation reactions |
US9938641B2 (en) | 2006-12-18 | 2018-04-10 | Fluidigm Corporation | Selection of aptamers based on geometry |
EP2336354A1 (en) * | 2009-12-18 | 2011-06-22 | Roche Diagnostics GmbH | A method for the detection of a RNA molecule, a kit and a use related thereof |
RU2578009C2 (ru) * | 2013-05-08 | 2016-03-20 | Закрытое акционерное общество "ЕВРОГЕН" | Способ идентификации нативных пар фрагментов днк или рнк, присутствующих в одних и тех же живых клетках |
RU2600873C1 (ru) * | 2015-06-24 | 2016-10-27 | Закрытое акционерное общество "ЕВРОГЕН" | Способ идентификации нативных пар фрагментов днк или рнк, присутствующих в одних и тех же живых клетках |
KR102468839B1 (ko) * | 2020-06-03 | 2022-11-18 | 주식회사 제노헬릭스 | Rna 검출 방법 |
Family Cites Families (8)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
ATE88761T1 (de) * | 1986-01-10 | 1993-05-15 | Amoco Corp | Kompetitiver homogener test. |
US5427930A (en) * | 1990-01-26 | 1995-06-27 | Abbott Laboratories | Amplification of target nucleic acids using gap filling ligase chain reaction |
CA2035010C (en) * | 1990-01-26 | 1996-12-10 | Keith C. Backman | Method of amplifying target nucleic acids applicable to both polymerase and ligase chain reactions |
US5210015A (en) * | 1990-08-06 | 1993-05-11 | Hoffman-La Roche Inc. | Homogeneous assay system using the nuclease activity of a nucleic acid polymerase |
US5348853A (en) * | 1991-12-16 | 1994-09-20 | Biotronics Corporation | Method for reducing non-specific priming in DNA amplification |
EP0633944B1 (en) * | 1992-03-31 | 2000-11-08 | Abbott Laboratories | Method of multiplex ligase chain reaction |
WO1994002648A1 (en) * | 1992-07-21 | 1994-02-03 | Imclone Systems Incorporated | Gap-filling nucleic acid amplification and detection |
US6180338B1 (en) * | 1992-08-04 | 2001-01-30 | Beckman Coulter, Inc. | Method, reagent and kit for the detection and amplification of nucleic acid sequences |
-
1994
- 1994-05-23 AT AT94916811T patent/ATE203775T1/de not_active IP Right Cessation
- 1994-05-23 NZ NZ266724A patent/NZ266724A/en unknown
- 1994-05-23 RU RU96124092/14A patent/RU2158765C2/ru active
- 1994-05-23 JP JP7530249A patent/JPH10500572A/ja not_active Ceased
- 1994-05-23 ES ES94916811T patent/ES2158895T3/es not_active Expired - Lifetime
- 1994-05-23 DK DK94916811T patent/DK0763133T3/da active
- 1994-05-23 DE DE69427876T patent/DE69427876T2/de not_active Expired - Fee Related
- 1994-05-23 AU AU68364/94A patent/AU706033B2/en not_active Ceased
- 1994-05-23 WO PCT/US1994/005767 patent/WO1995032306A1/en active IP Right Grant
- 1994-05-23 BR BR9408578A patent/BR9408578A/pt not_active IP Right Cessation
- 1994-05-23 EP EP94916811A patent/EP0763133B1/en not_active Expired - Lifetime
-
1997
- 1997-12-31 HK HK97102691A patent/HK1001131A1/xx not_active IP Right Cessation
-
2001
- 2001-10-26 GR GR20010401900T patent/GR3037030T3/el not_active IP Right Cessation
Also Published As
Publication number | Publication date |
---|---|
DE69427876T2 (de) | 2002-04-11 |
EP0763133B1 (en) | 2001-08-01 |
BR9408578A (pt) | 1997-08-19 |
GR3037030T3 (en) | 2002-01-31 |
EP0763133A1 (en) | 1997-03-19 |
DK0763133T3 (da) | 2001-10-08 |
HK1001131A1 (en) | 1998-05-29 |
ATE203775T1 (de) | 2001-08-15 |
RU2158765C2 (ru) | 2000-11-10 |
AU706033B2 (en) | 1999-06-10 |
DE69427876D1 (de) | 2001-09-06 |
AU6836494A (en) | 1995-12-18 |
NZ266724A (en) | 1998-08-26 |
JPH10500572A (ja) | 1998-01-20 |
EP0763133A4 (en) | 1999-02-10 |
WO1995032306A1 (en) | 1995-11-30 |
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Legal Events
Date | Code | Title | Description |
---|---|---|---|
FG2A | Definitive protection |
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