ES2157197T3 - Expresion de receptores acoplados a una proteina g en levadura. - Google Patents
Expresion de receptores acoplados a una proteina g en levadura.Info
- Publication number
- ES2157197T3 ES2157197T3 ES91916216T ES91916216T ES2157197T3 ES 2157197 T3 ES2157197 T3 ES 2157197T3 ES 91916216 T ES91916216 T ES 91916216T ES 91916216 T ES91916216 T ES 91916216T ES 2157197 T3 ES2157197 T3 ES 2157197T3
- Authority
- ES
- Spain
- Prior art keywords
- protein
- segment
- alpha
- yeast
- cell
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Expired - Lifetime
Links
Classifications
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
- G01N33/566—Immunoassay; Biospecific binding assay; Materials therefor using specific carrier or receptor proteins as ligand binding reagents where possible specific carrier or receptor proteins are classified with their target compounds
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
- C07K14/37—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from fungi
- C07K14/39—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from fungi from yeasts
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
- C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
- C07K14/46—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans from vertebrates
- C07K14/47—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans from vertebrates from mammals
- C07K14/4701—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans from vertebrates from mammals not used
- C07K14/4722—G-proteins
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
- C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
- C07K14/705—Receptors; Cell surface antigens; Cell surface determinants
- C07K14/70571—Receptors; Cell surface antigens; Cell surface determinants for neuromediators, e.g. serotonin receptor, dopamine receptor
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
- C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
- C12N15/80—Vectors or expression systems specially adapted for eukaryotic hosts for fungi
- C12N15/81—Vectors or expression systems specially adapted for eukaryotic hosts for fungi for yeasts
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6897—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids involving reporter genes operably linked to promoters
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K2319/00—Fusion polypeptide
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K2319/00—Fusion polypeptide
- C07K2319/01—Fusion polypeptide containing a localisation/targetting motif
- C07K2319/02—Fusion polypeptide containing a localisation/targetting motif containing a signal sequence
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K2319/00—Fusion polypeptide
- C07K2319/32—Fusion polypeptide fusions with soluble part of a cell surface receptor, "decoy receptors"
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K2319/00—Fusion polypeptide
- C07K2319/61—Fusion polypeptide containing an enzyme fusion for detection (lacZ, luciferase)
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K2319/00—Fusion polypeptide
- C07K2319/70—Fusion polypeptide containing domain for protein-protein interaction
- C07K2319/705—Fusion polypeptide containing domain for protein-protein interaction containing a protein-A fusion
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N2333/00—Assays involving biological materials from specific organisms or of a specific nature
- G01N2333/435—Assays involving biological materials from specific organisms or of a specific nature from animals; from humans
- G01N2333/705—Assays involving receptors, cell surface antigens or cell surface determinants
- G01N2333/72—Assays involving receptors, cell surface antigens or cell surface determinants for hormones
- G01N2333/726—G protein coupled receptor, e.g. TSHR-thyrotropin-receptor, LH/hCG receptor, FSH
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N2500/00—Screening for compounds of potential therapeutic value
Landscapes
- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Organic Chemistry (AREA)
- Genetics & Genomics (AREA)
- Engineering & Computer Science (AREA)
- Molecular Biology (AREA)
- Zoology (AREA)
- Biochemistry (AREA)
- General Health & Medical Sciences (AREA)
- Biomedical Technology (AREA)
- Biophysics (AREA)
- Immunology (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Biotechnology (AREA)
- Wood Science & Technology (AREA)
- Microbiology (AREA)
- Medicinal Chemistry (AREA)
- Mycology (AREA)
- General Engineering & Computer Science (AREA)
- Physics & Mathematics (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Gastroenterology & Hepatology (AREA)
- Analytical Chemistry (AREA)
- Cell Biology (AREA)
- Hematology (AREA)
- Urology & Nephrology (AREA)
- Toxicology (AREA)
- Food Science & Technology (AREA)
- Plant Pathology (AREA)
- General Physics & Mathematics (AREA)
- Pathology (AREA)
- Neurology (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Peptides Or Proteins (AREA)
- Preparation Of Compounds By Using Micro-Organisms (AREA)
Abstract
SE PRESENTA UNA CELULA DE LEVADURA TRANSFORMADA QUE CONTIENE UNA PRIMERA SECUENCIA DE DNA HOMOLOGO QUE CODIFICA UN RECEPTOR ACOPLADO A LA PROTEINA G DE UN MAMIFERO Y UNA SEGUNDA SECUENCIA HETEROLOGA DE DNA QUE CODIFICA UNA SUBUNIDAD (ALFA) DE MAMIFERO. LA PRIMERA Y LA SEGUNDA SECUENCIA HETEROLOGA DE DNA ES CAPAZ DE EXPRESARSE EN LA CELULA, PERO LA CELULA ES INCAPAZ DE EXPRESAR UNA SUBUNIDAD (ALFA) DE LA PROTEINA G ENDOGENA (G(ALFA) DE LA LEVADURA). LAS CELULAS SON UTILES PARA FILTRAR COMPUESTOS QUE SEAN ACEPTADOS POR LA PROPORCION DE DISOCIACION DE G(ALFA) A PARTIR DE G(BETA)GAMMA EN UNA CELULA. TAMBIEN SE PRESENTA UN NUEVO VECTOR DE EXPRESION DE DNA UTIL PARA OBTENER CELULAS COMO LAS DESCRITAS ANTERIORMENTE. EL VECTOR CONTIENE UN PRIMER SEGMENTO QUE CONTIENE AL MENOS UN FRAGMENTO DE FRECUENCIA QUE CODIFICA EL AMINO TERMINAL DEL EXTREMO DEL RECEPTOR ACOPLADO DE LA PROTEINA G DE LA LEVADURA. UN SEGUNDO SEGMENTO SE SITUA DEBAJO DEL PRIMER SEGMENTO (Y EN UNA ESTRUCTURA DE LECTURA COMPUESTA CONEL MISMO), COMPRENDIENDO EL SEGUNDO SEGMENTO UNA SECUENCIA DE DNA QUE CODIFICA UN RECEPTOR APROPIADO A LA PROTEINA G HETEROLOGA.
Applications Claiming Priority (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
US58171490A | 1990-09-13 | 1990-09-13 |
Publications (1)
Publication Number | Publication Date |
---|---|
ES2157197T3 true ES2157197T3 (es) | 2001-08-16 |
Family
ID=24326283
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
ES91916216T Expired - Lifetime ES2157197T3 (es) | 1990-09-13 | 1991-09-12 | Expresion de receptores acoplados a una proteina g en levadura. |
Country Status (12)
Country | Link |
---|---|
US (6) | US5482835A (es) |
EP (2) | EP0548165B1 (es) |
JP (3) | JP3348406B2 (es) |
AT (1) | ATE199391T1 (es) |
AU (1) | AU652576B2 (es) |
CA (1) | CA2092717C (es) |
DE (1) | DE69132546T2 (es) |
DK (1) | DK0548165T3 (es) |
ES (1) | ES2157197T3 (es) |
GR (1) | GR3035937T3 (es) |
IE (2) | IE20020110A1 (es) |
WO (1) | WO1992005244A1 (es) |
Families Citing this family (80)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
FR2628750B1 (fr) * | 1988-03-21 | 1991-11-08 | Pasteur Institut | Vecteur modifie par une sequence codant pour une sequence d'acides amines contenue dans une proteine de mammifere ayant une activite biologique de recepteur membranaire |
US6080540A (en) | 1990-04-20 | 2000-06-27 | Cold Spring Harbor Laboratory | Cloning of mammalian genes in microbial organisms and methods for pharmacological screening |
ATE199391T1 (de) * | 1990-09-13 | 2001-03-15 | Univ Duke | Expression von an g-protein gekoppelten rezeptoren in hefe |
US5264565A (en) * | 1991-01-22 | 1993-11-23 | Affymax Technologies, N.V. | Nucleic acid encoding the D2 /Ml chimeric receptor |
DE4138621A1 (de) * | 1991-11-25 | 1993-06-17 | Boehringer Ingelheim Int | Verfahren zum screenen von substanzen mit modulierender wirkung auf einen rezeptorabhaengigen zellulaeren signaluebertragungsweg |
US5688662A (en) * | 1992-04-09 | 1997-11-18 | Linguagen Corporation | Gustducin polynucleotides, vectors, host cells and recombinant methods |
US6255059B1 (en) | 1993-03-31 | 2001-07-03 | Cadus Pharmaceutical Corporation | Methods for identifying G protein coupled receptor effectors |
US5876951A (en) * | 1993-03-31 | 1999-03-02 | Cadus Pharmaceutical Corporation | Yeast cells engineered to produce pheromone system protein surrogates and uses therefor |
US7235648B1 (en) | 1993-03-31 | 2007-06-26 | Cadus Technologies, Inc. | Yeast cells engineered to produce pheromone system protein surrogates, and uses therefor |
US7416881B1 (en) | 1993-03-31 | 2008-08-26 | Cadus Technologies, Inc. | Yeast cells engineered to produce pheromone system protein surrogates, and uses therefor |
US5789184A (en) * | 1993-03-31 | 1998-08-04 | Cadus Pharmaceutical Corporation | Yeast cells engineered to produce pheromone system protein surrogates, and uses therefor |
US6100042A (en) | 1993-03-31 | 2000-08-08 | Cadus Pharmaceutical Corporation | Yeast cells engineered to produce pheromone system protein surrogates, and uses therefor |
US6864060B1 (en) | 1993-03-31 | 2005-03-08 | Cadus Technologies, Inc. | Yeast cells expressing modified G proteins and methods of use therefor |
ES2166371T3 (es) * | 1993-03-31 | 2002-04-16 | Cadus Pharmaceutical Corp | Celulas de levadura tratadas para producir sustitutos de proteinas de sistemas de feromonas y usos de las mismas. |
US20030054402A1 (en) | 1993-03-31 | 2003-03-20 | Cadus Pharmaceutical Corporation | Methods and compositions for identifying receptor effectors |
US6001581A (en) * | 1993-06-04 | 1999-12-14 | Sibia Neurosciences, Inc. | Cation-based bioassay using human metabotropic glutamate receptors |
IL110298A (en) * | 1993-07-13 | 1999-04-11 | Brann Mark Robert | Identification of ligands by selective amplification of cells transfected with receptors |
US5691188A (en) * | 1994-02-14 | 1997-11-25 | American Cyanamid Company | Transformed yeast cells expressing heterologous G-protein coupled receptor |
AU702075B2 (en) * | 1994-04-26 | 1999-02-11 | Cadus Pharmaceutical Corporation | Functional expression of mammalian adenylyl cyclase in yeast |
CA2220459A1 (en) * | 1995-06-07 | 1996-12-19 | Cadus Pharmaceutical Corporation | Expression of functional vertebrate phospholipases in yeast |
AU7115896A (en) * | 1995-09-20 | 1997-04-09 | Molecular Geriatrics Corporation | Yeast receptor and g-protein fusion protein |
US5851786A (en) * | 1995-09-27 | 1998-12-22 | National Jewish Center For Immunology And Respiratory Medicine | Product and process to regulate actin polymerization |
US7329741B2 (en) * | 1996-06-05 | 2008-02-12 | Chiron Corporation | Polynucleotides that hybridize to DP-75 and their use |
EP0938553B1 (en) | 1996-06-05 | 2009-08-05 | Novartis Vaccines and Diagnostics, Inc. | Dna encoding dp-75 and a process for its use |
US6083693A (en) | 1996-06-14 | 2000-07-04 | Curagen Corporation | Identification and comparison of protein-protein interactions that occur in populations |
JP2001501097A (ja) * | 1996-09-24 | 2001-01-30 | ケイダス ファーマスーティカル コーポレイション | レセプターエフェクターを同定する方法及び組成物 |
US6087115A (en) * | 1997-01-22 | 2000-07-11 | Cornell Research Foundation, Inc. | Methods of identifying negative antagonists for G protein coupled receptors |
US6403305B1 (en) * | 1997-02-06 | 2002-06-11 | Cornell Research Foundation, Inc. | Methods of identifying peptide agonists or negative antagonists of a G protein coupled receptor |
FR2764387B1 (fr) * | 1997-06-05 | 1999-07-23 | Centre Nat Rech Scient | Utilisation d'une proteine fluorescente pour la detection d'interactions entre une proteine cible et son ligand |
US5891646A (en) * | 1997-06-05 | 1999-04-06 | Duke University | Methods of assaying receptor activity and constructs useful in such methods |
US6528271B1 (en) | 1997-06-05 | 2003-03-04 | Duke University | Inhibition of βarrestin mediated effects prolongs and potentiates opioid receptor-mediated analgesia |
US7541151B2 (en) | 1997-06-05 | 2009-06-02 | Duke University | Single-cell biosensor for the measurement of GPCR ligands in a test sample |
CA2305204A1 (en) * | 1997-10-07 | 1999-04-15 | Cadus Pharmaceutical Corporation | Yeast cells expressing modified g proteins and methods of use therefor |
US6485922B1 (en) | 1997-10-10 | 2002-11-26 | Atairgin Technologies, Inc. | Methods for detecting compounds which modulate the activity of an LPA receptor |
WO1999019513A2 (en) * | 1997-10-10 | 1999-04-22 | Lxr Biotechnology, Inc. | Methods for detecting compounds which modulate the activity of an lpa receptor |
US6265174B1 (en) | 1997-11-03 | 2001-07-24 | Morphochem, Inc. | Methods and compositions for identifying and modulating ctionprotein-interactions |
GB9725462D0 (en) * | 1997-12-01 | 1998-01-28 | Smithkline Beecham Plc | Novel method |
US5955575A (en) * | 1997-12-22 | 1999-09-21 | Hopital Sainte-Justine | Antagonists of G-protein-coupled receptor |
WO1999051641A1 (en) * | 1998-04-03 | 1999-10-14 | Nps Pharmaceuticals, Inc. | G-protein fusion receptors and chimeric gabab receptors |
US7262280B1 (en) | 1998-04-03 | 2007-08-28 | Nps Pharmaceuticals, Inc. | G-protein fusion receptors and constructs encoding same |
GB9807722D0 (en) * | 1998-04-08 | 1998-06-10 | Smithkline Beecham Plc | Novel compounds |
JP2002513135A (ja) * | 1998-04-24 | 2002-05-08 | エール ユニヴァーシティ | 薬剤−受容体及びタンパク質−タンパク質相互作用を検出する方法 |
US6355473B1 (en) | 1998-05-06 | 2002-03-12 | Cadus Pharmaceutical Corp. | Yeast cells having mutations in stp22 and uses therefor |
US6733991B1 (en) | 1998-05-08 | 2004-05-11 | Osi Pharmaceuticals, Inc. | AGS proteins and nucleic acid molecules and uses therefor |
US6746852B1 (en) | 1998-05-08 | 2004-06-08 | Osi Pharmaceuticals, Inc. | AGS proteins and nucleic acid molecules and uses thereof |
US6280934B1 (en) | 1998-05-13 | 2001-08-28 | Millennium Pharmaceuticals, Inc. | Assay for agents which alter G-protein coupled receptor activity |
CA2334215A1 (en) * | 1998-06-05 | 1999-12-16 | Discovery Technologies Ltd. | Transformed cell lines which express heterologous g-protein-coupled receptors |
WO2000000515A2 (en) * | 1998-06-29 | 2000-01-06 | Hyseq, Inc. | A CHEMOKINE RECEPTOR OBTAINED FROM A cDNA LIBRARY OF FETAL LIVER-SPLEEN |
US7081360B2 (en) * | 1998-07-28 | 2006-07-25 | Cadus Technologies, Inc. | Expression of G protein-coupled receptors with altered ligand binding and/or coupling properties |
US7273747B2 (en) | 1998-08-27 | 2007-09-25 | Cadus Technologies, Inc. | Cell having amplified signal transduction pathway responses and uses therefor |
US7094593B1 (en) * | 1998-09-01 | 2006-08-22 | Basf Aktiengesellschaft | Method for improving the function of heterologous G protein-coupled receptors |
US6251605B1 (en) | 1998-10-27 | 2001-06-26 | Cadus Pharmaceutical Corporation | Yeast cells having mutations in Cav1 and uses therefor |
GB9824682D0 (en) * | 1998-11-10 | 1999-01-06 | Isis Innovation | Bacillus strain and assay method |
AU2030000A (en) | 1998-11-25 | 2000-06-13 | Cadus Pharmaceutical Corporation | Methods and compositions for identifying receptor effectors |
US6074845A (en) * | 1999-01-28 | 2000-06-13 | Smithkline Beecham Corporation | Nucleic acid encoding a bovine calcitonin receptor-like receptor (BECRLR) |
US6504008B1 (en) | 1999-02-01 | 2003-01-07 | Cadus Technologies, Inc. | Cell based signal generation |
US6555325B1 (en) | 1999-06-14 | 2003-04-29 | Cadus Technologies, Inc. | System for detection of a functional interaction between a compound and a cellular signal transduction component |
US7223533B2 (en) | 1999-09-10 | 2007-05-29 | Cadus Technologies, Inc. | Cell surface proteins and use thereof as indicators of activation of cellular signal transduction pathways |
IL147889A0 (en) * | 1999-09-24 | 2002-08-14 | Solvay Pharm Bv | Novel human g-protein coupled receptor |
US6998255B1 (en) * | 1999-09-24 | 2006-02-14 | Solvay Pharmaceuticals B.V. | Human G-protein coupled receptor |
US20050148018A1 (en) | 1999-10-07 | 2005-07-07 | David Weiner | Methods of identifying inverse agonists of the serotonin 2A receptor |
WO2001038874A1 (en) * | 1999-11-24 | 2001-05-31 | Millennium Pharmaceuticals, Inc. | Expression cloning method |
US7186505B1 (en) * | 1999-11-29 | 2007-03-06 | Stil Biotechnologies Ltd. | Expression systems and methods for detecting and isolating polypeptides regulating signal transduction pathways |
US7324236B2 (en) * | 2000-02-01 | 2008-01-29 | Canon Kabushiki Kaisha | Discrepancy correction method and apparatus for correcting difference in levels of image signals obtained by an image sensor having a multiple output channels |
US20050136431A1 (en) * | 2000-11-03 | 2005-06-23 | Oakley Robert H. | Methods of screening compositions for G protein-coupled receptor desensitization inhibitory activity |
US7163800B2 (en) * | 2000-11-03 | 2007-01-16 | Molecular Devices Corporation | Methods of screening compositions for G protein-coupled receptor desensitization inhibitory activity |
US7018812B2 (en) | 2000-11-03 | 2006-03-28 | Duke University | Modified G-protein coupled receptors |
AU2002243912A1 (en) * | 2001-02-08 | 2002-09-12 | Temple University-Of The Commonwealth System Of Higher Education | Biosensor for detecting chemical agents |
US7803982B2 (en) | 2001-04-20 | 2010-09-28 | The Mount Sinai School Of Medicine Of New York University | T1R3 transgenic animals, cells and related methods |
WO2002096946A1 (en) * | 2001-05-30 | 2002-12-05 | Bristol-Myers Squibb Company | Polynucleotide encoding a novel human g-protein coupled receptor, hgprbmy30 |
US20030232359A1 (en) * | 2002-03-18 | 2003-12-18 | Ramanathan Chandra S. | Polynucleotide encoding a novel human G-protein coupled receptor, HGPRBMY40_2 |
US20030182669A1 (en) * | 2002-03-19 | 2003-09-25 | Rockman Howard A. | Phosphoinositide 3-kinase mediated inhibition of GPCRs |
US7290215B2 (en) * | 2002-06-03 | 2007-10-30 | Microsoft Corporation | Dynamic wizard interface system and method |
AU2003212136A1 (en) * | 2003-03-07 | 2004-09-28 | National Research Council Of Canada | The determination of function of mammalian proteins by analysis of global gene expression profiles |
US20050186658A1 (en) * | 2003-10-17 | 2005-08-25 | Nps Pharmaceuticals, Inc. | Chimeric metabotropic glutamate receptors and uses thereof |
US7893041B2 (en) | 2003-12-05 | 2011-02-22 | Children's Hospital Medical Center | Oligosaccharide compositions and use thereof in the treatment of infection |
US20090221691A1 (en) * | 2005-03-07 | 2009-09-03 | University Of Rochester | Compositions and methods for inhibiting g protein signaling |
GB0520568D0 (en) | 2005-10-10 | 2005-11-16 | Paradigm Therapeutics Ltd | Receptor |
EP4365591A2 (en) | 2016-02-24 | 2024-05-08 | Suntory Holdings Limited | Biosensor detecting smell, scent and taste |
US10209239B1 (en) | 2017-08-16 | 2019-02-19 | Aromyx Corporation | Method of making an aromagraph comprising ectopic olfactory receptors |
Family Cites Families (37)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US4615974A (en) * | 1981-08-25 | 1986-10-07 | Celltech Limited | Yeast expression vectors |
US4865989A (en) * | 1981-12-22 | 1989-09-12 | U.C. Regents | Eukaryotic vectors and plasmids having PGK regulatory signals |
US4775622A (en) * | 1982-03-08 | 1988-10-04 | Genentech, Inc. | Expression, processing and secretion of heterologous protein by yeast |
US4546082A (en) * | 1982-06-17 | 1985-10-08 | Regents Of The Univ. Of California | E. coli/Saccharomyces cerevisiae plasmid cloning vector containing the alpha-factor gene for secretion and processing of hybrid proteins |
US4876197A (en) * | 1983-02-22 | 1989-10-24 | Chiron Corporation | Eukaryotic regulatable transcription |
US4661454A (en) | 1983-02-28 | 1987-04-28 | Collaborative Research, Inc. | GAL1 yeast promoter linked to non galactokinase gene |
NZ207926A (en) * | 1983-04-25 | 1988-04-29 | Genentech Inc | Use of yeast #a#-factor to assist in expression of proteins heterologus to yeast |
US4797359A (en) * | 1983-05-10 | 1989-01-10 | Board Of Regents, The University Of Texas System | Heat shock regulated production of selected and fused proteins in yeast |
US4880734A (en) * | 1984-05-11 | 1989-11-14 | Chiron Corporation | Eukaryotic regulatable transcription |
US4859609A (en) | 1986-04-30 | 1989-08-22 | Genentech, Inc. | Novel receptors for efficient determination of ligands and their antagonists or agonists |
US5071773A (en) | 1986-10-24 | 1991-12-10 | The Salk Institute For Biological Studies | Hormone receptor-related bioassays |
US5378603A (en) | 1987-03-30 | 1995-01-03 | Board Of Regents, The University Of Texas System | Method and composition for identifying substances which activate transcription of the LDL receptor gene |
US4952499A (en) | 1988-02-11 | 1990-08-28 | Dana-Farber Cancer Institute | Genes and their encoded proteins which regulate gene expression of the interleukin-2 receptor and of human lymphotropic retroviruses |
FR2628750B1 (fr) * | 1988-03-21 | 1991-11-08 | Pasteur Institut | Vecteur modifie par une sequence codant pour une sequence d'acides amines contenue dans une proteine de mammifere ayant une activite biologique de recepteur membranaire |
EP0424397B1 (en) | 1988-04-04 | 1996-06-19 | The Salk Institute Biotechnology Industrial Associates, Inc. | Calcium channel compositions and methods |
US5407820A (en) | 1988-04-04 | 1995-04-18 | The Salk Institute Biotechnology/Industrial Associates, Inc. | Calcium channel α-2 subunit DNAs and cells expressing them |
US5386025A (en) | 1990-02-20 | 1995-01-31 | The Salk Institute Biotechnology/Industrial Associates | Calcium channel compositions and methods |
US5747336A (en) | 1988-09-08 | 1998-05-05 | The United States Of America As Represented By The Department Of Health And Human Services | Cloned human genes for muscarinic acetylcholine receptors and cells lines expressing same |
EP0447483B1 (en) * | 1988-11-18 | 1997-06-18 | STATE OF OREGON, acting by and through THE OREGON STATE BOARD OF HIGHER EDUCATION, acting for and on behalf of | Dopamine receptors and genes |
US5217867A (en) * | 1988-11-30 | 1993-06-08 | The Salk Institute For Biological Studies | Receptors: their identification, characterization, preparation and use |
DE69034061D1 (de) | 1989-07-18 | 2003-05-28 | Osi Pharm Inc | Methode der veränderung der expression von genen bei der transkription und zum nachweis von chemischen substanzen, die wie modulatoren der genexpression wirken |
US5665543A (en) | 1989-07-18 | 1997-09-09 | Oncogene Science, Inc. | Method of discovering chemicals capable of functioning as gene expression modulators |
US5284746A (en) * | 1990-02-08 | 1994-02-08 | Zymogenetics, Inc. | Methods of producing hybrid G protein-coupled receptors |
US5369028A (en) | 1990-04-03 | 1994-11-29 | The Salk Institute Biotechnology/Industrial Associates, Inc. | DNA and mRNA encoding human neuronal nicotinic acetylcholine receptor compositions and cells transformed with same |
AU7686891A (en) * | 1990-04-05 | 1991-10-30 | American National Red Cross, The | A protein family related to immediate-early protein expressed by human endothelial cells during differentiation |
WO1991018005A1 (en) * | 1990-05-14 | 1991-11-28 | Duke University | Cloned genes encoding the d1 dopamine receptor |
US5426177A (en) | 1990-06-01 | 1995-06-20 | Regeneron Pharmaceuticals, Inc. | Ciliary neurotrophic factor receptor |
US5401629A (en) * | 1990-08-07 | 1995-03-28 | The Salk Institute Biotechnology/Industrial Associates, Inc. | Assay methods and compositions useful for measuring the transduction of an intracellular signal |
ATE199391T1 (de) * | 1990-09-13 | 2001-03-15 | Univ Duke | Expression von an g-protein gekoppelten rezeptoren in hefe |
NZ240921A (en) * | 1990-12-12 | 1994-06-27 | Zymogenetics Inc | G protein coupled glutamate receptor (neurotransmitters), recombinant production |
US5384243A (en) * | 1991-04-12 | 1995-01-24 | The United States Of America As Represented By The Department Of Health And Human Services | Method for screening an agent for its ability to prevent cell transformation |
US5215915A (en) * | 1991-04-16 | 1993-06-01 | Duke University | Cloned gene encoding rat d1b dopamine receptor |
US5245011A (en) * | 1991-04-16 | 1993-09-14 | Duke University | Aqueous solution containing D1B dopamine receptor |
WO1992019723A1 (en) * | 1991-04-29 | 1992-11-12 | The Trustees Of Boston University | Method of isolating ligands which bind neutrophil receptors |
CA2054602C (en) * | 1991-10-31 | 2003-04-22 | Anthony Pawson | Method for assaying for a substance that affects an sh2-phosphorylated ligand regulatory system |
US5364791A (en) * | 1992-05-14 | 1994-11-15 | Elisabetta Vegeto | Progesterone receptor having C. terminal hormone binding domain truncations |
US5468615A (en) * | 1993-07-01 | 1995-11-21 | The Upjohn Company | Binding assay employing a synthetic gene for D4 dopamine receptors |
-
1991
- 1991-09-12 AT AT91916216T patent/ATE199391T1/de not_active IP Right Cessation
- 1991-09-12 JP JP51518891A patent/JP3348406B2/ja not_active Expired - Fee Related
- 1991-09-12 EP EP91916216A patent/EP0548165B1/en not_active Expired - Lifetime
- 1991-09-12 DE DE69132546T patent/DE69132546T2/de not_active Expired - Fee Related
- 1991-09-12 WO PCT/US1991/006605 patent/WO1992005244A1/en active IP Right Grant
- 1991-09-12 CA CA002092717A patent/CA2092717C/en not_active Expired - Fee Related
- 1991-09-12 AU AU85115/91A patent/AU652576B2/en not_active Ceased
- 1991-09-12 EP EP00109507A patent/EP1029915A3/en not_active Withdrawn
- 1991-09-12 IE IE20020110A patent/IE20020110A1/en not_active IP Right Cessation
- 1991-09-12 ES ES91916216T patent/ES2157197T3/es not_active Expired - Lifetime
- 1991-09-12 DK DK91916216T patent/DK0548165T3/da active
- 1991-09-13 IE IE324291A patent/IE913242A1/en not_active IP Right Cessation
-
1993
- 1993-06-03 US US08/071,355 patent/US5482835A/en not_active Expired - Lifetime
-
1995
- 1995-05-15 US US08/441,291 patent/US5739029A/en not_active Expired - Lifetime
-
1998
- 1998-04-08 US US09/056,920 patent/US6168927B1/en not_active Expired - Lifetime
-
2000
- 2000-12-29 US US09/752,145 patent/US6855550B2/en not_active Expired - Fee Related
-
2001
- 2001-05-25 GR GR20010400791T patent/GR3035937T3/el not_active IP Right Cessation
-
2002
- 2002-03-04 JP JP2002057831A patent/JP2002253282A/ja active Pending
-
2003
- 2003-01-17 US US10/347,141 patent/US20030215887A1/en not_active Abandoned
- 2003-09-03 JP JP2003311757A patent/JP2004000282A/ja active Pending
-
2004
- 2004-11-23 US US10/997,069 patent/US7413876B2/en not_active Expired - Fee Related
Also Published As
Publication number | Publication date |
---|---|
US7413876B2 (en) | 2008-08-19 |
EP0548165A1 (en) | 1993-06-30 |
US6168927B1 (en) | 2001-01-02 |
EP1029915A3 (en) | 2004-11-17 |
EP0548165B1 (en) | 2001-02-28 |
US5739029A (en) | 1998-04-14 |
EP0548165A4 (en) | 1993-08-11 |
WO1992005244A1 (en) | 1992-04-02 |
DE69132546T2 (de) | 2001-10-04 |
DE69132546D1 (de) | 2001-04-05 |
EP1029915A2 (en) | 2000-08-23 |
DK0548165T3 (da) | 2001-07-16 |
IE20020110A1 (en) | 2003-05-28 |
JP2004000282A (ja) | 2004-01-08 |
US20050214739A1 (en) | 2005-09-29 |
US6855550B2 (en) | 2005-02-15 |
US20020022238A1 (en) | 2002-02-21 |
AU652576B2 (en) | 1994-09-01 |
CA2092717A1 (en) | 1992-03-14 |
JP2002253282A (ja) | 2002-09-10 |
IE913242A1 (en) | 1992-02-25 |
ATE199391T1 (de) | 2001-03-15 |
JP3348406B2 (ja) | 2002-11-20 |
JPH06500693A (ja) | 1994-01-27 |
US5482835A (en) | 1996-01-09 |
US20030215887A1 (en) | 2003-11-20 |
AU8511591A (en) | 1992-04-15 |
CA2092717C (en) | 2001-06-12 |
GR3035937T3 (en) | 2001-08-31 |
Similar Documents
Publication | Publication Date | Title |
---|---|---|
ES2157197T3 (es) | Expresion de receptores acoplados a una proteina g en levadura. | |
ES2172502T3 (es) | Receptores tipo ii de interleucina 1. | |
FI852547L (fi) | Framstaellning av faktor viii och daertill hoerande produkter. | |
DK119191D0 (da) | Kimaeriske immunoglobuliner, der er specifikke for p55 tac protein fra il-2 receptoren | |
MY102029A (en) | Human factor viii, compositions containing it, methods and materials for use in its production | |
ATE131871T1 (de) | Rezeptoren für tumornekrosisfaktor-alpha und - beta | |
IL98024A0 (en) | Cd44-surface proteins,dna-sequences coding therefor,polypeptides coded by said dna sequences and diagnostic compositions containing the same | |
TR200101592T2 (tr) | Faktör VIII ifade sistemi. | |
AR241654A1 (es) | Procedimiento para producir activador de plasminogeno de tejido humano. | |
DK0624199T3 (da) | Fremgangsmåder til fremstilling af thrombin | |
DK0479948T3 (da) | Sammensætninger og fremgangsmåder til syntese af natriuretisk protein-receptor B og anvendelsesfremgangsmåder | |
AU639226B2 (en) | Recombinant protein receptor | |
DK0775750T3 (da) | Fremstilling af proteiner ud fra pro-proteiner med fusionsproteiner afledt af furin eller furinanaloge | |
ATE177472T1 (de) | Expression von makrophagen-induzierbaren proteinen (mips) in hefezellen | |
DE69233463D1 (de) | Expression und reinigung von kloniertem humanen alpha-fetoprotein | |
ES2026206T3 (es) | Procedimiento para mejorar la expresion de proteina de eucariota. | |
DE69122864D1 (en) | Beta-zell-antigen | |
DK599788D0 (da) | Fremstilling af proteiner | |
Reeve et al. | Partial reduction of disulfide bonds in the hormone-specific subunits of TSH and LH | |
ZA973051B (en) | Type c lectins | |
ES8607392A1 (es) | Un procedimiento para producir un polipeptido que exhibe ac-tividad de factor de fijacion de ige de mamifero | |
ES8405842A1 (es) | Procedimiento de producir razas estables de celulas de mamiferos | |
EP0475182A3 (en) | Method for protein synthesis using cks fusion proteins | |
JPS57124970A (en) | Video information coding system |
Legal Events
Date | Code | Title | Description |
---|---|---|---|
FG2A | Definitive protection |
Ref document number: 548165 Country of ref document: ES |