ES2157197T3 - Expresion de receptores acoplados a una proteina g en levadura. - Google Patents

Expresion de receptores acoplados a una proteina g en levadura.

Info

Publication number
ES2157197T3
ES2157197T3 ES91916216T ES91916216T ES2157197T3 ES 2157197 T3 ES2157197 T3 ES 2157197T3 ES 91916216 T ES91916216 T ES 91916216T ES 91916216 T ES91916216 T ES 91916216T ES 2157197 T3 ES2157197 T3 ES 2157197T3
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ES
Spain
Prior art keywords
protein
segment
alpha
yeast
cell
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired - Lifetime
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ES91916216T
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English (en)
Inventor
Klim King
Henrik G Dohlman
Marc G Caron
Robert J Lefkowitz
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Duke University
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Duke University
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Application filed by Duke University filed Critical Duke University
Application granted granted Critical
Publication of ES2157197T3 publication Critical patent/ES2157197T3/es
Anticipated expiration legal-status Critical
Expired - Lifetime legal-status Critical Current

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    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/566—Immunoassay; Biospecific binding assay; Materials therefor using specific carrier or receptor proteins as ligand binding reagents where possible specific carrier or receptor proteins are classified with their target compounds
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/37—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from fungi
    • C07K14/39—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from fungi from yeasts
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
    • C07K14/46—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans from vertebrates
    • C07K14/47—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans from vertebrates from mammals
    • C07K14/4701—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans from vertebrates from mammals not used
    • C07K14/4722—G-proteins
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
    • C07K14/705—Receptors; Cell surface antigens; Cell surface determinants
    • C07K14/70571—Receptors; Cell surface antigens; Cell surface determinants for neuromediators, e.g. serotonin receptor, dopamine receptor
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/80—Vectors or expression systems specially adapted for eukaryotic hosts for fungi
    • C12N15/81—Vectors or expression systems specially adapted for eukaryotic hosts for fungi for yeasts
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6897—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids involving reporter genes operably linked to promoters
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C07K2319/01—Fusion polypeptide containing a localisation/targetting motif
    • C07K2319/02—Fusion polypeptide containing a localisation/targetting motif containing a signal sequence
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C07K2319/32—Fusion polypeptide fusions with soluble part of a cell surface receptor, "decoy receptors"
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C07K2319/61—Fusion polypeptide containing an enzyme fusion for detection (lacZ, luciferase)
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C07K2319/70—Fusion polypeptide containing domain for protein-protein interaction
    • C07K2319/705—Fusion polypeptide containing domain for protein-protein interaction containing a protein-A fusion
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N2333/00—Assays involving biological materials from specific organisms or of a specific nature
    • G01N2333/435—Assays involving biological materials from specific organisms or of a specific nature from animals; from humans
    • G01N2333/705—Assays involving receptors, cell surface antigens or cell surface determinants
    • G01N2333/72—Assays involving receptors, cell surface antigens or cell surface determinants for hormones
    • G01N2333/726—G protein coupled receptor, e.g. TSHR-thyrotropin-receptor, LH/hCG receptor, FSH
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N2500/00—Screening for compounds of potential therapeutic value

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  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Genetics & Genomics (AREA)
  • Engineering & Computer Science (AREA)
  • Molecular Biology (AREA)
  • Zoology (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Biomedical Technology (AREA)
  • Biophysics (AREA)
  • Immunology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Biotechnology (AREA)
  • Wood Science & Technology (AREA)
  • Microbiology (AREA)
  • Medicinal Chemistry (AREA)
  • Mycology (AREA)
  • General Engineering & Computer Science (AREA)
  • Physics & Mathematics (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Analytical Chemistry (AREA)
  • Cell Biology (AREA)
  • Hematology (AREA)
  • Urology & Nephrology (AREA)
  • Toxicology (AREA)
  • Food Science & Technology (AREA)
  • Plant Pathology (AREA)
  • General Physics & Mathematics (AREA)
  • Pathology (AREA)
  • Neurology (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Peptides Or Proteins (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)

Abstract

SE PRESENTA UNA CELULA DE LEVADURA TRANSFORMADA QUE CONTIENE UNA PRIMERA SECUENCIA DE DNA HOMOLOGO QUE CODIFICA UN RECEPTOR ACOPLADO A LA PROTEINA G DE UN MAMIFERO Y UNA SEGUNDA SECUENCIA HETEROLOGA DE DNA QUE CODIFICA UNA SUBUNIDAD (ALFA) DE MAMIFERO. LA PRIMERA Y LA SEGUNDA SECUENCIA HETEROLOGA DE DNA ES CAPAZ DE EXPRESARSE EN LA CELULA, PERO LA CELULA ES INCAPAZ DE EXPRESAR UNA SUBUNIDAD (ALFA) DE LA PROTEINA G ENDOGENA (G(ALFA) DE LA LEVADURA). LAS CELULAS SON UTILES PARA FILTRAR COMPUESTOS QUE SEAN ACEPTADOS POR LA PROPORCION DE DISOCIACION DE G(ALFA) A PARTIR DE G(BETA)GAMMA EN UNA CELULA. TAMBIEN SE PRESENTA UN NUEVO VECTOR DE EXPRESION DE DNA UTIL PARA OBTENER CELULAS COMO LAS DESCRITAS ANTERIORMENTE. EL VECTOR CONTIENE UN PRIMER SEGMENTO QUE CONTIENE AL MENOS UN FRAGMENTO DE FRECUENCIA QUE CODIFICA EL AMINO TERMINAL DEL EXTREMO DEL RECEPTOR ACOPLADO DE LA PROTEINA G DE LA LEVADURA. UN SEGUNDO SEGMENTO SE SITUA DEBAJO DEL PRIMER SEGMENTO (Y EN UNA ESTRUCTURA DE LECTURA COMPUESTA CONEL MISMO), COMPRENDIENDO EL SEGUNDO SEGMENTO UNA SECUENCIA DE DNA QUE CODIFICA UN RECEPTOR APROPIADO A LA PROTEINA G HETEROLOGA.
ES91916216T 1990-09-13 1991-09-12 Expresion de receptores acoplados a una proteina g en levadura. Expired - Lifetime ES2157197T3 (es)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
US58171490A 1990-09-13 1990-09-13

Publications (1)

Publication Number Publication Date
ES2157197T3 true ES2157197T3 (es) 2001-08-16

Family

ID=24326283

Family Applications (1)

Application Number Title Priority Date Filing Date
ES91916216T Expired - Lifetime ES2157197T3 (es) 1990-09-13 1991-09-12 Expresion de receptores acoplados a una proteina g en levadura.

Country Status (12)

Country Link
US (6) US5482835A (es)
EP (2) EP0548165B1 (es)
JP (3) JP3348406B2 (es)
AT (1) ATE199391T1 (es)
AU (1) AU652576B2 (es)
CA (1) CA2092717C (es)
DE (1) DE69132546T2 (es)
DK (1) DK0548165T3 (es)
ES (1) ES2157197T3 (es)
GR (1) GR3035937T3 (es)
IE (2) IE20020110A1 (es)
WO (1) WO1992005244A1 (es)

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US7413876B2 (en) 2008-08-19
EP0548165A1 (en) 1993-06-30
US6168927B1 (en) 2001-01-02
EP1029915A3 (en) 2004-11-17
EP0548165B1 (en) 2001-02-28
US5739029A (en) 1998-04-14
EP0548165A4 (en) 1993-08-11
WO1992005244A1 (en) 1992-04-02
DE69132546T2 (de) 2001-10-04
DE69132546D1 (de) 2001-04-05
EP1029915A2 (en) 2000-08-23
DK0548165T3 (da) 2001-07-16
IE20020110A1 (en) 2003-05-28
JP2004000282A (ja) 2004-01-08
US20050214739A1 (en) 2005-09-29
US6855550B2 (en) 2005-02-15
US20020022238A1 (en) 2002-02-21
AU652576B2 (en) 1994-09-01
CA2092717A1 (en) 1992-03-14
JP2002253282A (ja) 2002-09-10
IE913242A1 (en) 1992-02-25
ATE199391T1 (de) 2001-03-15
JP3348406B2 (ja) 2002-11-20
JPH06500693A (ja) 1994-01-27
US5482835A (en) 1996-01-09
US20030215887A1 (en) 2003-11-20
AU8511591A (en) 1992-04-15
CA2092717C (en) 2001-06-12
GR3035937T3 (en) 2001-08-31

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