ES2154648T3 - Deteccion y amplificacion de acidos nucleicos blanco con actividad exonucleolitica. - Google Patents
Deteccion y amplificacion de acidos nucleicos blanco con actividad exonucleolitica.Info
- Publication number
- ES2154648T3 ES2154648T3 ES93917324T ES93917324T ES2154648T3 ES 2154648 T3 ES2154648 T3 ES 2154648T3 ES 93917324 T ES93917324 T ES 93917324T ES 93917324 T ES93917324 T ES 93917324T ES 2154648 T3 ES2154648 T3 ES 2154648T3
- Authority
- ES
- Spain
- Prior art keywords
- amplification
- exonucleolitic
- activity
- detection
- nucleic acids
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Expired - Lifetime
Links
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6844—Nucleic acid amplification reactions
- C12Q1/6862—Ligase chain reaction [LCR]
Landscapes
- Chemical & Material Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Organic Chemistry (AREA)
- Engineering & Computer Science (AREA)
- Zoology (AREA)
- Wood Science & Technology (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Health & Medical Sciences (AREA)
- Biophysics (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Immunology (AREA)
- Microbiology (AREA)
- Molecular Biology (AREA)
- Analytical Chemistry (AREA)
- Physics & Mathematics (AREA)
- Biotechnology (AREA)
- Biochemistry (AREA)
- Bioinformatics & Cheminformatics (AREA)
- General Engineering & Computer Science (AREA)
- General Health & Medical Sciences (AREA)
- Genetics & Genomics (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
- Saccharide Compounds (AREA)
Abstract
LA INVENCION SE REFIERE A ESQUEMAS MEJORADOS DE AMPLIFICACION LCR QUE UTILIZAN AL MENOS UNA SONDA MODIFICADA EN SU EXTREMO 5'' PARA REDUCIR O ELIMINAR LA AMPLIFICACION INDEPENDIENTE DEL OBJETIVO. TAMBIEN SE PRESENTAN LAS SONDAS DIFERENTES, MODIFICADAS Y LOS EQUIPOS QUE LAS CONTIENEN. TAMBIEN SE PRESENTA UN METODO PARA DETECTAR LAS DIFERENCIAS EN SECUENCIAS DE ACIDO NUCLEICO, CON UNA AMPLIFICACION REDUCIDA INDEPENDIENTE DEL OBJETIVO, UTILIZANDO LAS ONDAS MODIFICADAS.
Applications Claiming Priority (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
US92540292A | 1992-08-03 | 1992-08-03 |
Publications (1)
Publication Number | Publication Date |
---|---|
ES2154648T3 true ES2154648T3 (es) | 2001-04-16 |
Family
ID=25451687
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
ES93917324T Expired - Lifetime ES2154648T3 (es) | 1992-08-03 | 1993-07-21 | Deteccion y amplificacion de acidos nucleicos blanco con actividad exonucleolitica. |
Country Status (7)
Country | Link |
---|---|
EP (1) | EP0654093B1 (es) |
JP (1) | JP3516953B2 (es) |
AU (1) | AU4687393A (es) |
CA (1) | CA2140331C (es) |
DE (1) | DE69329824T2 (es) |
ES (1) | ES2154648T3 (es) |
WO (1) | WO1994003636A1 (es) |
Families Citing this family (7)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US5593840A (en) * | 1993-01-27 | 1997-01-14 | Oncor, Inc. | Amplification of nucleic acid sequences |
US5888731A (en) * | 1995-08-30 | 1999-03-30 | Visible Genetics Inc. | Method for identification of mutations using ligation of multiple oligonucleotide probes |
US6852487B1 (en) | 1996-02-09 | 2005-02-08 | Cornell Research Foundation, Inc. | Detection of nucleic acid sequence differences using the ligase detection reaction with addressable arrays |
EP1736554B1 (en) | 1996-05-29 | 2013-10-09 | Cornell Research Foundation, Inc. | Detection of nucleic acid sequence differences using coupled ligase detection and polymerase chain reactions |
GB9902000D0 (en) | 1999-01-30 | 1999-03-17 | Delta Biotechnology Ltd | Process |
JP2004507230A (ja) * | 2000-07-03 | 2004-03-11 | アプレラ コーポレイション | ポリヌクレオチド配列アッセイ |
ATE431407T1 (de) * | 2002-01-11 | 2009-05-15 | Biospring Ges Fuer Biotechnolo | Verfahren zur herstellung von dna |
Family Cites Families (6)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
DE68927373T2 (de) * | 1988-06-24 | 1997-03-20 | Amgen Inc | Verfahren und mittel zum nachweis von nukleinsäuresequenzen |
US5427930A (en) * | 1990-01-26 | 1995-06-27 | Abbott Laboratories | Amplification of target nucleic acids using gap filling ligase chain reaction |
CA2035010C (en) * | 1990-01-26 | 1996-12-10 | Keith C. Backman | Method of amplifying target nucleic acids applicable to both polymerase and ligase chain reactions |
DK0528882T3 (da) * | 1990-05-03 | 2008-01-14 | Cornell Res Foundation Inc | DNA-amplifikationssystem til påvisning af genetiske sygdomme ved hjælp af termostabil ligase |
US5210015A (en) * | 1990-08-06 | 1993-05-11 | Hoffman-La Roche Inc. | Homogeneous assay system using the nuclease activity of a nucleic acid polymerase |
WO1993024656A1 (en) * | 1992-05-29 | 1993-12-09 | Abbott Laboratories | Ligase chain reaction starting with rna sequences |
-
1993
- 1993-07-21 WO PCT/US1993/006931 patent/WO1994003636A1/en active IP Right Grant
- 1993-07-21 EP EP93917324A patent/EP0654093B1/en not_active Expired - Lifetime
- 1993-07-21 DE DE69329824T patent/DE69329824T2/de not_active Expired - Fee Related
- 1993-07-21 CA CA002140331A patent/CA2140331C/en not_active Expired - Fee Related
- 1993-07-21 JP JP50536994A patent/JP3516953B2/ja not_active Expired - Fee Related
- 1993-07-21 ES ES93917324T patent/ES2154648T3/es not_active Expired - Lifetime
- 1993-07-21 AU AU46873/93A patent/AU4687393A/en not_active Abandoned
Also Published As
Publication number | Publication date |
---|---|
JP3516953B2 (ja) | 2004-04-05 |
EP0654093A4 (en) | 1998-01-07 |
DE69329824D1 (de) | 2001-02-08 |
EP0654093B1 (en) | 2001-01-03 |
CA2140331A1 (en) | 1994-02-17 |
CA2140331C (en) | 2000-01-18 |
AU4687393A (en) | 1994-03-03 |
WO1994003636A1 (en) | 1994-02-17 |
DE69329824T2 (de) | 2001-08-09 |
EP0654093A1 (en) | 1995-05-24 |
JPH08501212A (ja) | 1996-02-13 |
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Legal Events
Date | Code | Title | Description |
---|---|---|---|
FG2A | Definitive protection |
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