ES2152933T3 - Determinacion de huellas relativas a cepas bacterianas utilizando amplificacion de secuencias de adn repetitivas. - Google Patents

Determinacion de huellas relativas a cepas bacterianas utilizando amplificacion de secuencias de adn repetitivas.

Info

Publication number
ES2152933T3
ES2152933T3 ES92923440T ES92923440T ES2152933T3 ES 2152933 T3 ES2152933 T3 ES 2152933T3 ES 92923440 T ES92923440 T ES 92923440T ES 92923440 T ES92923440 T ES 92923440T ES 2152933 T3 ES2152933 T3 ES 2152933T3
Authority
ES
Spain
Prior art keywords
bacteria
initiators
amplification
bacterial
quality
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired - Lifetime
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ES92923440T
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English (en)
Inventor
James R Lupski
Thearith Koeuth
James Versalovic
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Baylor College of Medicine
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Baylor College of Medicine
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Application filed by Baylor College of Medicine filed Critical Baylor College of Medicine
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Publication of ES2152933T3 publication Critical patent/ES2152933T3/es
Anticipated expiration legal-status Critical
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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813—Hybridisation assays
    • C12Q1/6827—Hybridisation assays for detection of mutation or polymorphism
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844—Nucleic acid amplification reactions
    • C12Q1/6858—Allele-specific amplification
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6876—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6876—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes
    • C12Q1/6888—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for detection or identification of organisms
    • C12Q1/689—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for detection or identification of organisms for bacteria
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2600/00—Oligonucleotides characterized by their use
    • C12Q2600/16—Primer sets for multiplex assays

Landscapes

  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Health & Medical Sciences (AREA)
  • Zoology (AREA)
  • Engineering & Computer Science (AREA)
  • Wood Science & Technology (AREA)
  • Analytical Chemistry (AREA)
  • Immunology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Molecular Biology (AREA)
  • Biophysics (AREA)
  • Biotechnology (AREA)
  • Physics & Mathematics (AREA)
  • Biochemistry (AREA)
  • Microbiology (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Saccharide Compounds (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)

Abstract

SE PRESENTAN METODOS E INICIADORES OLIGONUCLEOTIDOS PARA IDENTIFICAR CLASES DE BACTERIAS MEDIANTE SU IMPRESION GENOMICA. LOS METODOS SON APLICABLES EN UNA VARIEDAD DE MUESTRAS. EL PROCEDIMIENTO DE PRUEBA INCLUYE LA AMPLIFICACION DEL DNA BACTERIANO EN LA MUESTRA A COMPROBAR MEDIANTE LA ADICION DE UN PAR DE INICIADORES EXTERIORMENTE DIRIGIDOS PARA LA MUESTRA. LOS INICIADORES SON CAPACES DE HIBRIDIZARSE CON SECUENCIAS REPETITIVAS DE DNA EN EL DNA BACTERIANO Y SE EXTIENDEN DE MANERA EXTERIOR A LA SECUENCIA REPETITIVA HIBRIDIZABLE HASTA OTRA SECUENCIA REPETITIVA HIBRIDIZABLE. DESPUES DE LA AMPLIFICACION LOS PRODUCTOS DE EXTENSION SE SEPARAN POR EL TAMAÑO Y LA CLASE ESPECIFICA DE LA BACTERIA SE DETERMINA MIDIENDO LA FORMA DE LOS PRODUCTOS DE EXTENSION SEPARADOS POR TAMAÑOS. EL PROCEDIMIENTO PARA IDENTIFICAR CLASES DE BACTERIAS MEDIANTE SU IMPRESION GENOMICA TIENE UNA VARIEDAD DE USOS QUE INCLUYEN: LA IDENTIFICACION DE BACTERIAS EN INFECCIONES, PLANES DE AGRICULTURA Y HORTICULTURA, MONITORIZACIONDE ALIMENTOS, MONITORIZACION DE LA PRODUCCION, SEGURIDAD EN LA CALIDAD Y CONTROL DE CALIDAD.
ES92923440T 1991-10-23 1992-10-21 Determinacion de huellas relativas a cepas bacterianas utilizando amplificacion de secuencias de adn repetitivas. Expired - Lifetime ES2152933T3 (es)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
US78142491A 1991-10-23 1991-10-23

Publications (1)

Publication Number Publication Date
ES2152933T3 true ES2152933T3 (es) 2001-02-16

Family

ID=25122697

Family Applications (1)

Application Number Title Priority Date Filing Date
ES92923440T Expired - Lifetime ES2152933T3 (es) 1991-10-23 1992-10-21 Determinacion de huellas relativas a cepas bacterianas utilizando amplificacion de secuencias de adn repetitivas.

Country Status (9)

Country Link
US (2) US5691136A (es)
EP (1) EP0610396B1 (es)
AT (1) ATE198773T1 (es)
AU (1) AU2931692A (es)
CA (1) CA2121696C (es)
DE (1) DE69231646T2 (es)
DK (1) DK0610396T3 (es)
ES (1) ES2152933T3 (es)
WO (1) WO1993008297A1 (es)

Cited By (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
ES2193862A1 (es) * 2001-12-20 2003-11-01 Univ Cordoba Metodo para el analisis molecular de la diversidad genetica en razas de fusarium oxysporum f. sp. ciceris mediante rep-pcr.
ES2193861A1 (es) * 2001-12-20 2003-11-01 Univ Cordoba Metodo para la identificacion molecular de fusarium oxysporum f. sp. ciceris y sus razas patogenicas 0,5 y 6 mediante pcr especifica.
ES2330820A1 (es) * 2007-11-23 2009-12-15 Universidad Complutense De Madrid Metodos y compuestos para la caracterizacion de la poblacion bacteriana en biopeliculas.

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CA2121696C (en) 2003-07-08
EP0610396B1 (en) 2001-01-17
CA2121696A1 (en) 1993-04-29
DE69231646D1 (de) 2001-02-22
WO1993008297A1 (en) 1993-04-29
US5523217A (en) 1996-06-04
EP0610396A4 (en) 1997-01-08
US5691136A (en) 1997-11-25
DE69231646T2 (de) 2001-06-13
AU2931692A (en) 1993-05-21
EP0610396A1 (en) 1994-08-17
ATE198773T1 (de) 2001-02-15

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