ES2149672B1 - HUMAN GENE SEQUENCE APPROVES THE AFC1 GENE OF LEAVES INVOLVED IN THE PROTEOLITIC PROCESSING OF PRENILED PROTEINS. - Google Patents

HUMAN GENE SEQUENCE APPROVES THE AFC1 GENE OF LEAVES INVOLVED IN THE PROTEOLITIC PROCESSING OF PRENILED PROTEINS.

Info

Publication number
ES2149672B1
ES2149672B1 ES9800016A ES9800016A ES2149672B1 ES 2149672 B1 ES2149672 B1 ES 2149672B1 ES 9800016 A ES9800016 A ES 9800016A ES 9800016 A ES9800016 A ES 9800016A ES 2149672 B1 ES2149672 B1 ES 2149672B1
Authority
ES
Spain
Prior art keywords
sequence
afc1
proteins
gene
human gene
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired - Fee Related
Application number
ES9800016A
Other languages
Spanish (es)
Other versions
ES2149672A1 (en
Inventor
Freije Jose Maria Perez
Cotarelo Gloria Velasco
Pendas Alberto Martin
Albors Pilar Blay
Felechosa Milagros Balbin
Otin Carlos Lopez
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Universidad de Oviedo
Original Assignee
Universidad de Oviedo
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Universidad de Oviedo filed Critical Universidad de Oviedo
Priority to ES9901608A priority Critical patent/ES2151459B1/en
Priority to ES9800016A priority patent/ES2149672B1/en
Priority to PCT/ES1999/000001 priority patent/WO1999035275A1/en
Publication of ES2149672A1 publication Critical patent/ES2149672A1/en
Application granted granted Critical
Publication of ES2149672B1 publication Critical patent/ES2149672B1/en
Anticipated expiration legal-status Critical
Expired - Fee Related legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/48—Hydrolases (3) acting on peptide bonds (3.4)
    • C12N9/50—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25)
    • C12N9/64—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from animal tissue
    • C12N9/6421—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from animal tissue from mammals
    • C12N9/6489—Metalloendopeptidases (3.4.24)
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/574—Immunoassay; Biospecific binding assay; Materials therefor for cancer
    • G01N33/57484—Immunoassay; Biospecific binding assay; Materials therefor for cancer involving compounds serving as markers for tumor, cancer, neoplasia, e.g. cellular determinants, receptors, heat shock/stress proteins, A-protein, oligosaccharides, metabolites
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K38/00—Medicinal preparations containing peptides

Landscapes

  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Chemical & Material Sciences (AREA)
  • Biomedical Technology (AREA)
  • Molecular Biology (AREA)
  • Immunology (AREA)
  • Wood Science & Technology (AREA)
  • Biotechnology (AREA)
  • Hematology (AREA)
  • Microbiology (AREA)
  • Organic Chemistry (AREA)
  • Urology & Nephrology (AREA)
  • Zoology (AREA)
  • Cell Biology (AREA)
  • Medicinal Chemistry (AREA)
  • Genetics & Genomics (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • General Physics & Mathematics (AREA)
  • Pathology (AREA)
  • Analytical Chemistry (AREA)
  • Physics & Mathematics (AREA)
  • Food Science & Technology (AREA)
  • Hospice & Palliative Care (AREA)
  • General Engineering & Computer Science (AREA)
  • Oncology (AREA)
  • Peptides Or Proteins (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)

Abstract

Secuencia génica humana homóloga al gen de levaduras implicado en el procesamiento proteolítico de proteínas preniladas. La invención consiste en identificar fragmentos homólogos de gen humano a AFC1 de Saccharomyces cerevisiae, amplificarlos mediante PCR de ARN total, utilizar los fragmentos amplificados como sondas para hibridar ADNc y determinar la secuencia de los clones de ADNc que hibriden con las sondas. La secuencia identificada es SEQ ID NO: 1. Las aplicaciones de dicha secuencia están relacionadas fundamentalmente con la diagnosis y el tratamiento de procesos oncogénicos.Human gene sequence homologous to the yeast gene involved in proteolytic processing of pre-packed proteins. The invention consists in identifying homologous fragments of the human gene to Saccharomyces cerevisiae AFC1, amplifying them by total RNA PCR, using the amplified fragments as probes to hybridize cDNAs and determining the sequence of the cDNA clones that hybridize with the probes. The sequence identified is SEQ ID NO: 1. The applications of said sequence are mainly related to the diagnosis and treatment of oncogenic processes.

ES9800016A 1998-01-08 1998-01-08 HUMAN GENE SEQUENCE APPROVES THE AFC1 GENE OF LEAVES INVOLVED IN THE PROTEOLITIC PROCESSING OF PRENILED PROTEINS. Expired - Fee Related ES2149672B1 (en)

Priority Applications (3)

Application Number Priority Date Filing Date Title
ES9901608A ES2151459B1 (en) 1998-01-08 1998-01-08 HUMAN GENE SEQUENCE APPROVES THE RCE1 GENE OF YEAST INVOLVED IN THE PROTEOLITIC PROCESSING OF PRENILED PROTEINS.
ES9800016A ES2149672B1 (en) 1998-01-08 1998-01-08 HUMAN GENE SEQUENCE APPROVES THE AFC1 GENE OF LEAVES INVOLVED IN THE PROTEOLITIC PROCESSING OF PRENILED PROTEINS.
PCT/ES1999/000001 WO1999035275A1 (en) 1998-01-08 1999-01-08 Human genetic sequences which are homologous to the yeast genes involved in the proteolytic processing of prenylated proteins

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
ES9800016A ES2149672B1 (en) 1998-01-08 1998-01-08 HUMAN GENE SEQUENCE APPROVES THE AFC1 GENE OF LEAVES INVOLVED IN THE PROTEOLITIC PROCESSING OF PRENILED PROTEINS.

Publications (2)

Publication Number Publication Date
ES2149672A1 ES2149672A1 (en) 2000-11-01
ES2149672B1 true ES2149672B1 (en) 2001-05-16

Family

ID=8302331

Family Applications (2)

Application Number Title Priority Date Filing Date
ES9800016A Expired - Fee Related ES2149672B1 (en) 1998-01-08 1998-01-08 HUMAN GENE SEQUENCE APPROVES THE AFC1 GENE OF LEAVES INVOLVED IN THE PROTEOLITIC PROCESSING OF PRENILED PROTEINS.
ES9901608A Expired - Fee Related ES2151459B1 (en) 1998-01-08 1998-01-08 HUMAN GENE SEQUENCE APPROVES THE RCE1 GENE OF YEAST INVOLVED IN THE PROTEOLITIC PROCESSING OF PRENILED PROTEINS.

Family Applications After (1)

Application Number Title Priority Date Filing Date
ES9901608A Expired - Fee Related ES2151459B1 (en) 1998-01-08 1998-01-08 HUMAN GENE SEQUENCE APPROVES THE RCE1 GENE OF YEAST INVOLVED IN THE PROTEOLITIC PROCESSING OF PRENILED PROTEINS.

Country Status (2)

Country Link
ES (2) ES2149672B1 (en)
WO (1) WO1999035275A1 (en)

Family Cites Families (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CA2262517A1 (en) * 1996-08-07 1998-02-12 The Regents Of The University Of California Afc1 and rce1:isoprenylated caax processing enzymes
WO1998054333A2 (en) * 1997-06-02 1998-12-03 Acacia Biosciences Inc. Mammalian caax processing enzymes
CA2234418A1 (en) * 1997-06-24 1998-12-24 Smithkline Beecham Corporation Human afc1
CA2234402A1 (en) * 1997-06-24 1998-12-24 Smithkline Beecham Corporation Human rce1

Also Published As

Publication number Publication date
ES2151459A1 (en) 2000-12-16
ES2149672A1 (en) 2000-11-01
WO1999035275A1 (en) 1999-07-15
ES2151459B1 (en) 2001-07-01

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