ES2144139T3 - Genes de la penicilina g-acilasa mutados. - Google Patents

Genes de la penicilina g-acilasa mutados.

Info

Publication number
ES2144139T3
ES2144139T3 ES95929882T ES95929882T ES2144139T3 ES 2144139 T3 ES2144139 T3 ES 2144139T3 ES 95929882 T ES95929882 T ES 95929882T ES 95929882 T ES95929882 T ES 95929882T ES 2144139 T3 ES2144139 T3 ES 2144139T3
Authority
ES
Spain
Prior art keywords
penicillin
acilase
mutated
genes
acylases
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired - Lifetime
Application number
ES95929882T
Other languages
English (en)
Inventor
Der Laan Jan Metske Van
Wilhelmus Johannes Quax
Adriana Marina Riemens
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Koninklijke DSM NV
Original Assignee
DSM NV
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by DSM NV filed Critical DSM NV
Application granted granted Critical
Publication of ES2144139T3 publication Critical patent/ES2144139T3/es
Anticipated expiration legal-status Critical
Expired - Lifetime legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P35/00—Preparation of compounds having a 5-thia-1-azabicyclo [4.2.0] octane ring system, e.g. cephalosporin
    • C12P35/02—Preparation of compounds having a 5-thia-1-azabicyclo [4.2.0] octane ring system, e.g. cephalosporin by desacylation of the substituent in the 7 position
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/78—Hydrolases (3) acting on carbon to nitrogen bonds other than peptide bonds (3.5)
    • C12N9/80—Hydrolases (3) acting on carbon to nitrogen bonds other than peptide bonds (3.5) acting on amide bonds in linear amides (3.5.1)
    • C12N9/84—Penicillin amidase (3.5.1.11)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P37/00—Preparation of compounds having a 4-thia-1-azabicyclo [3.2.0] heptane ring system, e.g. penicillin
    • C12P37/06—Preparation of compounds having a 4-thia-1-azabicyclo [3.2.0] heptane ring system, e.g. penicillin by desacylation of the substituent in the 6 position

Landscapes

  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Zoology (AREA)
  • Engineering & Computer Science (AREA)
  • Wood Science & Technology (AREA)
  • Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Microbiology (AREA)
  • Biochemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Biotechnology (AREA)
  • General Chemical & Material Sciences (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Mycology (AREA)
  • Medicinal Chemistry (AREA)
  • Molecular Biology (AREA)
  • Biomedical Technology (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Saccharide Compounds (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)

Abstract

SE PROPORCIONAN NUEVOS MUTANTES BE -LACTAMA DE ACILASA DE PENICILINA G, QUE PRESENTAN ESPECIFICIDADES DE SUSTRATO ALTERADAS. DICHAS ACILASAS DE PENICILINA G, SE OBTIENEN MEDIANTE EXPRESION DE UN GEN QUE CODIFICA PARA DICHA ACILASA DE PENICILINA G, LA CUAL TIENE UNA SECUENCIA AMINOACIDA QUE DIFIERE AL MENOS EN UN AMINOACIDO DE LA ACILASA DE PENICILINA G DE TIPO SILVESTRE.
ES95929882T 1994-08-12 1995-08-14 Genes de la penicilina g-acilasa mutados. Expired - Lifetime ES2144139T3 (es)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
EP94202314 1994-08-12

Publications (1)

Publication Number Publication Date
ES2144139T3 true ES2144139T3 (es) 2000-06-01

Family

ID=8217100

Family Applications (1)

Application Number Title Priority Date Filing Date
ES95929882T Expired - Lifetime ES2144139T3 (es) 1994-08-12 1995-08-14 Genes de la penicilina g-acilasa mutados.

Country Status (10)

Country Link
US (2) US5891703A (es)
EP (1) EP0775210B1 (es)
JP (1) JP4147319B2 (es)
CN (1) CN1159444C (es)
AT (1) ATE189477T1 (es)
AU (1) AU3346295A (es)
DE (1) DE69514923T2 (es)
ES (1) ES2144139T3 (es)
MX (1) MX9701025A (es)
WO (1) WO1996005318A1 (es)

Families Citing this family (31)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP4147319B2 (ja) * 1994-08-12 2008-09-10 コニンクリーケ デーエスエム ナムローゼ フェンノートシャップ 変異ペニシリンｇアシラーゼ遺伝子
AU718648B2 (en) 1996-11-05 2000-04-20 Bristol-Myers Squibb Company Mutant penicillin G acylases
EP1315827A1 (en) * 2000-09-08 2003-06-04 Dsm N.V. Method for the preparation of enantiomerically enriched amines
BR0215329A (pt) * 2001-12-27 2004-11-16 Dsm Ip Assets Bv Processo para preparação de um antibiótico de b-lactama
JP4113427B2 (ja) * 2002-08-16 2008-07-09 富士フイルム株式会社 熱現像感光材料
DE10251747A1 (de) * 2002-11-05 2004-05-19 Tuhh-Technologie-Gmbh Gentechnisch veränderte Penicillinamidase und Verfahren zu ihrer Herstellung
WO2004111241A1 (en) * 2003-06-12 2004-12-23 Stichting Voor De Technische Wetenschappen Penicillin acylase
KR100530299B1 (ko) * 2003-08-11 2005-11-22 산도즈 게엠베하 변이 세팔로스포린 ｃ 아실라제 및 이를 이용한 7-ａｃａ 제조방법
ITMI20040016A1 (it) * 2004-01-12 2004-04-12 Antibiotics S P A Cefalosporina c acilasi
CN101177688B (zh) * 2006-11-08 2010-12-01 中国科学院上海生命科学研究院 突变青霉素g酰化酶、其重组表达质粒及转化的工程菌株
CZ300467B6 (cs) * 2007-01-31 2009-05-27 Mikrobiologický ústav AV CR, v.v.i. Sekvence nukleotidu o velikosti 2646 bp, kódující penicilinacylázu, nové konstrukty rekombinantní nukleové kyseliny a rekombinantní mikroorganismy nesoucí tuto sekvenci
WO2010027710A2 (en) 2008-08-27 2010-03-11 Codexis, Inc. Ketoreductase polypeptides and uses thereof
SI2329013T1 (sl) * 2008-08-27 2016-03-31 Codexis, Inc. Polipeptidi ketoreduktaze za proizvodnjo 3-aril-3-hidroksipropanamina iz 3-aril-3-ketopropanamina
WO2010025287A2 (en) * 2008-08-27 2010-03-04 Codexis, Inc. Ketoreductase polypeptides for the production of 3-aryl-3-hydroxypropanamine from a 3-aryl-3-ketopropanamine
EP2329014B1 (en) 2008-08-29 2014-10-22 Codexis, Inc. Ketoreductase polypeptides for the stereoselective production of (4s)-3[(5s)-5(4-fluorophenyl)-5-hydroxypentanoyl]-4-phenyl-1,3-oxazolidin-2-one
WO2010054319A2 (en) * 2008-11-10 2010-05-14 Codexis, Inc. Penicillin-g acylases
EP2367940B1 (en) 2008-12-23 2017-11-22 DSM Sinochem Pharmaceuticals Netherlands B.V. Mutant penicillin G acylases
EP2467473B1 (en) 2009-08-19 2016-03-23 Codexis, Inc. Ketoreductase polypeptides for the preparation of phenylephrine
US9040262B2 (en) 2010-05-04 2015-05-26 Codexis, Inc. Biocatalysts for ezetimibe synthesis
EP2723881A2 (en) 2011-06-23 2014-04-30 DSM Sinochem Pharmaceuticals Netherlands B.V. Novel crystalline cefoperazone intermediate
CN103635585A (zh) 2011-06-23 2014-03-12 中化帝斯曼制药有限公司荷兰公司 用青霉素g酰化酶制备3’-硫代头孢菌素的方法
WO2013057197A1 (en) 2011-10-20 2013-04-25 Dsm Sinochem Pharmaceuticals Netherlands B.V. Process for the formylation of cefamandole
WO2013057196A1 (en) 2011-10-20 2013-04-25 Dsm Sinochem Pharmaceuticals Netherlands B.V. Process for the preparation of cefamandole nafate
RU2537845C2 (ru) * 2012-04-25 2015-01-10 Федеральное государственное бюджетное образовательное учреждение высшего профессиональногообразования "Московский государственный университет имени М.В. Ломоносова " (МГУ) Способ синтеза пептидов, в том числе бета-лактамных антибиотиков, при использовании варианта пенициллинацилазы
CN103667418B (zh) * 2013-12-18 2015-03-25 华北制药河北华民药业有限责任公司 高活性β-内酰胺抗生素合成用酶的高通量筛选方法
JP6379531B2 (ja) * 2014-03-10 2018-08-29 東洋紡株式会社 新規β−アミノ酸の製造方法
HUE053863T2 (hu) 2015-05-07 2021-07-28 Codexis Inc Penicillin-G acilázok
WO2017186864A1 (en) 2016-04-27 2017-11-02 Sandoz Ag Enzymatic process for the production of beta-lactam antibiotics in the presence of particulate inoculum
CN111051506B (zh) * 2017-06-27 2023-11-24 科德克希思公司 青霉素g酰化酶
CN110343734B (zh) * 2019-06-14 2021-03-23 浙江工业大学 一种l-草铵膦化学酶法生产方法
CN111172142B (zh) * 2020-02-14 2021-09-28 上海陶宇晟生物技术有限责任公司 一种热稳定性高的头孢菌素c酰化酶突变体

Family Cites Families (11)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JPS6121097A (ja) * 1984-07-10 1986-01-29 Asahi Chem Ind Co Ltd ７−アミノセフアロスポラン酸及びその誘導体の製造法
US4981789A (en) * 1987-03-18 1991-01-01 Merck & Co., Inc. One-step enzymatic conversion of cephalosporin C and derivatives to 7-aminocephalosporanic acid and derivatives
EP0322032A3 (en) * 1987-12-21 1991-01-30 Merck & Co. Inc. One-step enzymatic conversion of cephalosporin c and derivatives to 7-aminocephalosporanic acid and derivatives
PT97409B (pt) * 1990-04-18 1998-08-31 Gist Brocades Nv Processo para a preparacao de mutantes beta-lactama-acilases por expressao genetica
KR0152667B1 (ko) * 1990-04-18 1998-10-01 한스 월터 라벤 페니실린 g 아실라아제, 이 효소를 코드화한 유전자 및 이 효소의 제조방법
GB9019724D0 (en) * 1990-09-10 1990-10-24 Fujisawa Pharmaceutical Co Cephalosporin c acylase
GB9204439D0 (en) * 1992-02-27 1992-04-15 Fujisawa Pharmaceutical Co A new cephalosporin c acylase
TW400384B (en) * 1993-11-01 2000-08-01 Fujisawa Pharmaceutical Co A new cephalosporin C acylase
JP4147319B2 (ja) * 1994-08-12 2008-09-10 コニンクリーケ デーエスエム ナムローゼ フェンノートシャップ 変異ペニシリンｇアシラーゼ遺伝子
US5516679A (en) * 1994-12-23 1996-05-14 Bristol-Myers Squibb Company Penicillin V amidohydrolase gene from Fusarium oxysporum
AU718648B2 (en) * 1996-11-05 2000-04-20 Bristol-Myers Squibb Company Mutant penicillin G acylases

Also Published As

Publication number Publication date
DE69514923D1 (de) 2000-03-09
AU3346295A (en) 1996-03-07
CN1159444C (zh) 2004-07-28
WO1996005318A1 (en) 1996-02-22
JPH10507072A (ja) 1998-07-14
ATE189477T1 (de) 2000-02-15
US6033823A (en) 2000-03-07
CN1158638A (zh) 1997-09-03
JP4147319B2 (ja) 2008-09-10
DE69514923T2 (de) 2000-11-02
EP0775210A1 (en) 1997-05-28
US5891703A (en) 1999-04-06
EP0775210B1 (en) 2000-02-02
MX9701025A (es) 1997-05-31

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