ES2142720B1 - GLUTEN QUANTIFICATION PROCEDURE IN FOOD. - Google Patents
GLUTEN QUANTIFICATION PROCEDURE IN FOOD.Info
- Publication number
- ES2142720B1 ES2142720B1 ES9700518A ES9700518A ES2142720B1 ES 2142720 B1 ES2142720 B1 ES 2142720B1 ES 9700518 A ES9700518 A ES 9700518A ES 9700518 A ES9700518 A ES 9700518A ES 2142720 B1 ES2142720 B1 ES 2142720B1
- Authority
- ES
- Spain
- Prior art keywords
- gluten
- food
- dry
- quantification procedure
- procedure
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Expired - Fee Related
Links
Landscapes
- Other Investigation Or Analysis Of Materials By Electrical Means (AREA)
Abstract
Procedimiento de cuantificación del gluten en alimentos. Procedimiento que se realiza por espectrometría de masas MALDI-TOF: 160 {u l de extracto etanólico de muestra junto con 32 {u l de octil-{b -D-glucopiranósido 50 mM se mezclan con 100 {u l de disolución saturada de ácido sinapínico en acetonitrilo acuoso al 30% con 0,1% de ácido trifluoroacético, se seca a una alicota de 25 {u l en Speed-Vac hasta reducir su volumen a 3-4 {u l (aprox. 10 min) un de esta mezcla se deposita sobre el pocillo de un portamuestras de acero inoxidable y se deja secar a temperatura ambiente durante 5 min.Quantification procedure for gluten in food. Procedure performed by MALDI-TOF mass spectrometry: 160 {ul of sample ethanolic extract together with 32 {ul of 50 mM octyl- {b -D-glucopyranoside are mixed with 100 {ul of saturated solution of synapinic acid in acetonitrile 30% aqueous with 0.1% trifluoroacetic acid, dry to a 25 {ul aliquot in Speed-Vac until reducing its volume to 3-4 {ul (approx. 10 min) one of this mixture is deposited on the well of a stainless steel specimen holder and allowed to dry at room temperature for 5 min.
Priority Applications (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
ES9700518A ES2142720B1 (en) | 1997-03-10 | 1997-03-10 | GLUTEN QUANTIFICATION PROCEDURE IN FOOD. |
Applications Claiming Priority (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
ES9700518A ES2142720B1 (en) | 1997-03-10 | 1997-03-10 | GLUTEN QUANTIFICATION PROCEDURE IN FOOD. |
Publications (2)
Publication Number | Publication Date |
---|---|
ES2142720A1 ES2142720A1 (en) | 2000-04-16 |
ES2142720B1 true ES2142720B1 (en) | 2000-12-01 |
Family
ID=8298572
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
ES9700518A Expired - Fee Related ES2142720B1 (en) | 1997-03-10 | 1997-03-10 | GLUTEN QUANTIFICATION PROCEDURE IN FOOD. |
Country Status (1)
Country | Link |
---|---|
ES (1) | ES2142720B1 (en) |
Families Citing this family (2)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
BR0200690A (en) * | 2002-03-04 | 2004-03-23 | Embrapa Pesquisa Agropecuaria | Method for detection of source proteins in complex mixtures |
ES2402286B1 (en) | 2011-09-29 | 2014-03-04 | Universidad De Valladolid | IMMUNOGENIC PEPTIDE OF GLUTEN AND ITS APPLICATIONS. |
-
1997
- 1997-03-10 ES ES9700518A patent/ES2142720B1/en not_active Expired - Fee Related
Also Published As
Publication number | Publication date |
---|---|
ES2142720A1 (en) | 2000-04-16 |
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Legal Events
Date | Code | Title | Description |
---|---|---|---|
EC2A | Search report published |
Date of ref document: 20000416 Kind code of ref document: A1 Effective date: 20000416 |
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FD2A | Announcement of lapse in spain |
Effective date: 20180807 |