ES2134166A1 - Mejoras introducidas en metodos de extraccion de acidos nucleicos. - Google Patents
Mejoras introducidas en metodos de extraccion de acidos nucleicos.Info
- Publication number
- ES2134166A1 ES2134166A1 ES09702621A ES9702621A ES2134166A1 ES 2134166 A1 ES2134166 A1 ES 2134166A1 ES 09702621 A ES09702621 A ES 09702621A ES 9702621 A ES9702621 A ES 9702621A ES 2134166 A1 ES2134166 A1 ES 2134166A1
- Authority
- ES
- Spain
- Prior art keywords
- sediment
- methods
- nucleic acids
- extraction
- medium
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Granted
Links
Landscapes
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
Abstract
Mejoras introducidas en métodos de extracción de ácidos nucléicos. Dichos métodos se basan en la formación de un sedimento, que contiene los ácidos nucléicos, por precipitación con agentes precipitantes previa lisis de las células a analizar, seguida de posterior centrifugación y resuspensión final de dicho sedimento para la detección de los referidos ácidos. Las mejoras se caracterizan porque (12) se introducen en el recipiente que contiene el sedimento partículas sólidas de un material que no interfiera con el medio y (29) se agita vigorosamente el conjunto hasta la total disgregación y resuspensión del sedimento en el medio líquido en que se encuentra. Aplicación en el sector analítico de la Biología Molecular.
Priority Applications (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
ES9702621A ES2134166B1 (es) | 1997-12-17 | 1997-12-17 | Mejoras introducidas en metodos de extraccion de acidos nucleicos. |
Applications Claiming Priority (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
ES9702621A ES2134166B1 (es) | 1997-12-17 | 1997-12-17 | Mejoras introducidas en metodos de extraccion de acidos nucleicos. |
Publications (2)
Publication Number | Publication Date |
---|---|
ES2134166A1 true ES2134166A1 (es) | 1999-09-16 |
ES2134166B1 ES2134166B1 (es) | 2000-04-16 |
Family
ID=8301532
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
ES9702621A Expired - Lifetime ES2134166B1 (es) | 1997-12-17 | 1997-12-17 | Mejoras introducidas en metodos de extraccion de acidos nucleicos. |
Country Status (1)
Country | Link |
---|---|
ES (1) | ES2134166B1 (es) |
Citations (3)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US4090263A (en) * | 1975-02-28 | 1978-05-16 | Beckman Instruments, Inc. | Magnetic mixing apparatus |
US4911556A (en) * | 1989-03-17 | 1990-03-27 | Lim Technology Laboratories, Inc. | Turbulent stirring unit |
EP0442026A2 (en) * | 1990-02-14 | 1991-08-21 | Talent SRL | Method to extract and purify human genomic DNA |
-
1997
- 1997-12-17 ES ES9702621A patent/ES2134166B1/es not_active Expired - Lifetime
Patent Citations (4)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US4090263A (en) * | 1975-02-28 | 1978-05-16 | Beckman Instruments, Inc. | Magnetic mixing apparatus |
US4911556A (en) * | 1989-03-17 | 1990-03-27 | Lim Technology Laboratories, Inc. | Turbulent stirring unit |
EP0442026A2 (en) * | 1990-02-14 | 1991-08-21 | Talent SRL | Method to extract and purify human genomic DNA |
US5596092A (en) * | 1990-02-14 | 1997-01-21 | Talent S.R.L. | Extraction of genomic DNA from blood using cationic detergents |
Non-Patent Citations (1)
Title |
---|
NG, WAI-LAP et al. "High-throughput plasmid mini preparations facilitated by micro-mixing". NUCLEIC ACIDS RESEARCH, 1996, Vol. 24, No 24, paginas 5045-5047 * |
Also Published As
Publication number | Publication date |
---|---|
ES2134166B1 (es) | 2000-04-16 |
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Legal Events
Date | Code | Title | Description |
---|---|---|---|
EC2A | Search report published |
Date of ref document: 19990916 Kind code of ref document: A1 Effective date: 19990916 |
|
FA2A | Application withdrawn |
Effective date: 20001025 |