ES2134166A1 - Mejoras introducidas en metodos de extraccion de acidos nucleicos. - Google Patents

Mejoras introducidas en metodos de extraccion de acidos nucleicos.

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Publication number
ES2134166A1
ES2134166A1 ES09702621A ES9702621A ES2134166A1 ES 2134166 A1 ES2134166 A1 ES 2134166A1 ES 09702621 A ES09702621 A ES 09702621A ES 9702621 A ES9702621 A ES 9702621A ES 2134166 A1 ES2134166 A1 ES 2134166A1
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ES
Spain
Prior art keywords
sediment
methods
nucleic acids
extraction
medium
Prior art date
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Granted
Application number
ES09702621A
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English (en)
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ES2134166B1 (es
Inventor
Senor Solicitante El
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Individual
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Individual
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Priority to ES9702621A priority Critical patent/ES2134166B1/es
Publication of ES2134166A1 publication Critical patent/ES2134166A1/es
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Publication of ES2134166B1 publication Critical patent/ES2134166B1/es
Anticipated expiration legal-status Critical
Expired - Lifetime legal-status Critical Current

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Abstract

Mejoras introducidas en métodos de extracción de ácidos nucléicos. Dichos métodos se basan en la formación de un sedimento, que contiene los ácidos nucléicos, por precipitación con agentes precipitantes previa lisis de las células a analizar, seguida de posterior centrifugación y resuspensión final de dicho sedimento para la detección de los referidos ácidos. Las mejoras se caracterizan porque (12) se introducen en el recipiente que contiene el sedimento partículas sólidas de un material que no interfiera con el medio y (29) se agita vigorosamente el conjunto hasta la total disgregación y resuspensión del sedimento en el medio líquido en que se encuentra. Aplicación en el sector analítico de la Biología Molecular.
ES9702621A 1997-12-17 1997-12-17 Mejoras introducidas en metodos de extraccion de acidos nucleicos. Expired - Lifetime ES2134166B1 (es)

Priority Applications (1)

Application Number Priority Date Filing Date Title
ES9702621A ES2134166B1 (es) 1997-12-17 1997-12-17 Mejoras introducidas en metodos de extraccion de acidos nucleicos.

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
ES9702621A ES2134166B1 (es) 1997-12-17 1997-12-17 Mejoras introducidas en metodos de extraccion de acidos nucleicos.

Publications (2)

Publication Number Publication Date
ES2134166A1 true ES2134166A1 (es) 1999-09-16
ES2134166B1 ES2134166B1 (es) 2000-04-16

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ID=8301532

Family Applications (1)

Application Number Title Priority Date Filing Date
ES9702621A Expired - Lifetime ES2134166B1 (es) 1997-12-17 1997-12-17 Mejoras introducidas en metodos de extraccion de acidos nucleicos.

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ES (1) ES2134166B1 (es)

Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4090263A (en) * 1975-02-28 1978-05-16 Beckman Instruments, Inc. Magnetic mixing apparatus
US4911556A (en) * 1989-03-17 1990-03-27 Lim Technology Laboratories, Inc. Turbulent stirring unit
EP0442026A2 (en) * 1990-02-14 1991-08-21 Talent SRL Method to extract and purify human genomic DNA

Patent Citations (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4090263A (en) * 1975-02-28 1978-05-16 Beckman Instruments, Inc. Magnetic mixing apparatus
US4911556A (en) * 1989-03-17 1990-03-27 Lim Technology Laboratories, Inc. Turbulent stirring unit
EP0442026A2 (en) * 1990-02-14 1991-08-21 Talent SRL Method to extract and purify human genomic DNA
US5596092A (en) * 1990-02-14 1997-01-21 Talent S.R.L. Extraction of genomic DNA from blood using cationic detergents

Non-Patent Citations (1)

* Cited by examiner, † Cited by third party
Title
NG, WAI-LAP et al. "High-throughput plasmid mini preparations facilitated by micro-mixing". NUCLEIC ACIDS RESEARCH, 1996, Vol. 24, No 24, paginas 5045-5047 *

Also Published As

Publication number Publication date
ES2134166B1 (es) 2000-04-16

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