ES2132087T3 - Plasmidios que contienen adn que codifica la secuencia de aminoacidos de tcf-ii, celulas transformadas y produccion de la sustancia fisiologicamente activa usando las mismas. - Google Patents

Plasmidios que contienen adn que codifica la secuencia de aminoacidos de tcf-ii, celulas transformadas y produccion de la sustancia fisiologicamente activa usando las mismas.

Info

Publication number
ES2132087T3
ES2132087T3 ES91912323T ES91912323T ES2132087T3 ES 2132087 T3 ES2132087 T3 ES 2132087T3 ES 91912323 T ES91912323 T ES 91912323T ES 91912323 T ES91912323 T ES 91912323T ES 2132087 T3 ES2132087 T3 ES 2132087T3
Authority
ES
Spain
Prior art keywords
tcf
complementary dna
plasmide
transformed cells
codes
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired - Lifetime
Application number
ES91912323T
Other languages
English (en)
Inventor
Nobuyuki Shima
Kanji Higashio
Masaya Nagao
Fumiko Oogaki
Hiroaki Takaoka
Eisuke Maronie Haitsu Tsuda
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Snow Brand Milk Products Co Ltd
Original Assignee
Snow Brand Milk Products Co Ltd
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Snow Brand Milk Products Co Ltd filed Critical Snow Brand Milk Products Co Ltd
Application granted granted Critical
Publication of ES2132087T3 publication Critical patent/ES2132087T3/es
Anticipated expiration legal-status Critical
Expired - Lifetime legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
    • C07K14/475—Growth factors; Growth regulators
    • C07K14/4753—Hepatocyte growth factor; Scatter factor; Tumor cytotoxic factor II
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
    • C12N1/20—Bacteria; Culture media therefor
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/10—Cells modified by introduction of foreign genetic material
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P21/00—Preparation of peptides or proteins
    • C12P21/02—Preparation of peptides or proteins having a known sequence of two or more amino acids, e.g. glutathione

Landscapes

  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Engineering & Computer Science (AREA)
  • Genetics & Genomics (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biotechnology (AREA)
  • Biomedical Technology (AREA)
  • General Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • Molecular Biology (AREA)
  • General Engineering & Computer Science (AREA)
  • Microbiology (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Medicinal Chemistry (AREA)
  • Biophysics (AREA)
  • Toxicology (AREA)
  • Physics & Mathematics (AREA)
  • Plant Pathology (AREA)
  • Cell Biology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • General Chemical & Material Sciences (AREA)
  • Tropical Medicine & Parasitology (AREA)
  • Virology (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Peptides Or Proteins (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)
  • Saccharide Compounds (AREA)

Abstract

SE PREPARA UNA BIBLIOTECA DE ADN COMPLEMENTARIO EXTRAYENDO LA TOTALIDAD DE ARN MENSAJERO DE FIBROBLASTOS DE PULMON DE FETO HUMANO. DE ESTA BIBLIOTECA SE CLONA UN ADN COMPLEMENTARIO QUE CODIFICA TCF-II A LO LARGO DE TODA SU LONGITUD UTILIZANDO UNA SONDA DE OLIGONUCLEOTIDO SINTETICA QUE CODIFICA UN AMINOACIDO TERMINAL N, Y SE DETERMINA TODA LA SECUENCIA BASE DEL ADN COMPLEMENTARIO. SE EXPRESA UNA GRAN CANTIDAD DE TCF-II EN CELULAS DE MAMIFERO TRANSFORMADAS MEDIANTE PLASMIDA QUE CONTIENE EL ADN COMPLEMENTARIO Y SE PURIFICA EL TCF-II ASI DERIVADO DE LAS CELULAS TRANSFORMADAS. EL TCF-II PURIFICADO ES UTIL COMO FACTOR DE CRECIMIENTO DE CELULA HEPATICA O FACTOR ACTIVO DE DESTRUCCION DE CELULA TUMORAL EN EL CAMPO MEDICO O SIMILAR. A... PLASMIDA QUE EXPRESA TCF-II B... PROMOTOR CNV/T7 C... SELECCION PF D... PUNTO DE INICIACION DE REPLICACION E... SEÑAL DE UNION MAS SEÑAL DE POLI (A) ADICION F... TRATAMIENTO DE TERMINAL LISO SEGUN KLENOV G... PLASMIDA QUE EXPRESA TCF-II EN GRAN CANTIDAD
ES91912323T 1990-07-13 1991-07-15 Plasmidios que contienen adn que codifica la secuencia de aminoacidos de tcf-ii, celulas transformadas y produccion de la sustancia fisiologicamente activa usando las mismas. Expired - Lifetime ES2132087T3 (es)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
JP18585290 1990-07-13

Publications (1)

Publication Number Publication Date
ES2132087T3 true ES2132087T3 (es) 1999-08-16

Family

ID=16178009

Family Applications (1)

Application Number Title Priority Date Filing Date
ES91912323T Expired - Lifetime ES2132087T3 (es) 1990-07-13 1991-07-15 Plasmidios que contienen adn que codifica la secuencia de aminoacidos de tcf-ii, celulas transformadas y produccion de la sustancia fisiologicamente activa usando las mismas.

Country Status (10)

Country Link
US (1) US5328836A (es)
EP (1) EP0539590B1 (es)
JP (1) JP2634323B2 (es)
KR (1) KR0153009B1 (es)
AT (1) ATE178354T1 (es)
CA (1) CA2066618C (es)
DE (1) DE69131069T2 (es)
DK (1) DK0539590T3 (es)
ES (1) ES2132087T3 (es)
WO (1) WO1992001053A1 (es)

Families Citing this family (24)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
NZ232813A (en) * 1989-03-10 1992-08-26 Snow Brand Milk Products Co Ltd Human fibroblast glycoprotein, cell differentiation, blood vessel endothelial cell growth factor, cellular immunology inforcing factor of 78 or 74 thousand daltons plus or minus two thousand daltons
US5821223A (en) * 1990-09-14 1998-10-13 The United States Of America As Represented By The Department Of Health And Human Services Method of stimulating cell growth with a novel broad spectrum human lung fibroblast-derived mitogen
DE69230879T2 (de) * 1991-07-26 2000-08-03 Toray Industries, Inc. Verwendung von Hepatocyten-Wachstumsfaktoren zur Herstellung eines Vermehrungssystems für Hematopoietische Stammzellen
WO1993023541A1 (en) * 1992-05-18 1993-11-25 Genentech, Inc. Hepatocyte growth factor variants
US5328837A (en) * 1992-05-18 1994-07-12 Genentech, Inc. Hepatocyte growth factor protease domain variants
US5316921A (en) * 1992-05-18 1994-05-31 Genentech, Inc. Single-chain hepatocyte growth factor variants
CA2140010C (en) * 1992-07-16 2008-04-01 Hiroaki Masunaga Blood coagulation normalizer containing tcf-ii as active ingredient
ES2276409T3 (es) 1992-07-16 2007-06-16 Daiichi Pharmaceutical Co., Ltd. Composicion medica que contiene tcf-ii.
JP3402476B2 (ja) * 1992-08-24 2003-05-06 生化学工業株式会社 リポ多糖結合性タンパク質及びその製造法
JP3552240B2 (ja) * 1993-02-23 2004-08-11 第一製薬株式会社 高濃度ｔｃｆ製剤
IT1271088B (it) * 1994-11-24 1997-05-26 Dompe Spa Uso di fattore di crescita degli epatociti per indurre la proliferazione e differenziazione di cellule emopoietiche
NZ298142A (en) * 1994-12-27 1997-08-22 Snow Brand Milk Products Co Ltd Tcf mutant
KR19980020025A (ko) * 1996-09-05 1998-06-25 김종진 미생물을 이용한 폐수중의 나프탈렌 제거 방법
JP4006058B2 (ja) 1997-03-11 2007-11-14 第一三共株式会社 多臓器不全予防及び／又は治療剤
WO1998041230A1 (fr) 1997-03-14 1998-09-24 Snow Brand Milk Products Co., Ltd. Agent preventif et/ou curatif de la cachexie
KR100562824B1 (ko) * 2002-03-20 2006-03-23 주식회사 바이로메드 유전자 발현효율이 높으며 간세포 성장인자의 두 가지이형체를 동시에 발현하는 하이브리드 간세포 성장인자유전자
EP1814575A4 (en) * 2004-11-24 2010-07-07 Neopro Labs Llc METHODS AND COMPOSITIONS FOR TREATING DISORDERS
US7557088B2 (en) * 2006-03-28 2009-07-07 Neopro Labs, Llc Methods and compositions for treating conditions
WO2008144675A1 (en) 2007-05-17 2008-11-27 Neopro Labs, Llc Crystalline and amorphous forms of peptide
WO2009012472A2 (en) * 2007-07-18 2009-01-22 Neopro Labs, Llc Methods and compositions for treating conditions
US20090202606A1 (en) * 2008-01-25 2009-08-13 Viromed Co., Ltd. Treatment and Prevention of Cardiac Conditions Using Two or More Isoforms of Hepatocyte Growth Factor
RU2470995C2 (ru) * 2008-04-09 2012-12-27 Вайромед Ко., Лтд. Лиофилизированные днк-составы для увеличенной экспрессии плазмидной днк
AU2014337870B2 (en) 2013-10-22 2019-11-28 Helixmith Co., Ltd. Composition for preventing or treating amyotrophic lateral sclerosis using two or more isoforms of hepatocyte growth factor
EP3823677A4 (en) 2018-07-19 2022-06-01 Helixmith Co., Ltd. FREEZE-DRIED PHARMACEUTICAL COMPOSITIONS INTENDED FOR NAKED-DNA GENE THERAPY

Family Cites Families (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JPS62171696A (ja) * 1986-01-23 1987-07-28 Sumitomo Chem Co Ltd ヒトエリスロポエチンの製造方法
NZ232813A (en) * 1989-03-10 1992-08-26 Snow Brand Milk Products Co Ltd Human fibroblast glycoprotein, cell differentiation, blood vessel endothelial cell growth factor, cellular immunology inforcing factor of 78 or 74 thousand daltons plus or minus two thousand daltons
CA2022752C (en) * 1989-08-11 1998-07-07 Naomi Kitamura Hepatic parenchymal cell growth factor, gene encoding the same, process for producing the factor, and transformants producing the factor
JP2777678B2 (ja) * 1990-06-11 1998-07-23 敏一 中村 組換ヒト肝実質細胞増殖因子及びその製造方法

Also Published As

Publication number Publication date
KR920702417A (ko) 1992-09-04
CA2066618A1 (en) 1992-01-14
EP0539590B1 (en) 1999-03-31
DK0539590T3 (da) 1999-10-11
ATE178354T1 (de) 1999-04-15
JP2634323B2 (ja) 1997-07-23
EP0539590A4 (es) 1995-05-24
WO1992001053A1 (fr) 1992-01-23
DE69131069D1 (de) 1999-05-06
DE69131069T2 (de) 1999-07-15
EP0539590A1 (en) 1993-05-05
US5328836A (en) 1994-07-12
KR0153009B1 (ko) 1998-10-15
CA2066618C (en) 1998-05-19

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