ES2123818T3 - Procedimiento para medir las variaciones metaplasicas de los epitelocitos mucosecretores. - Google Patents

Procedimiento para medir las variaciones metaplasicas de los epitelocitos mucosecretores.

Info

Publication number
ES2123818T3
ES2123818T3 ES94925314T ES94925314T ES2123818T3 ES 2123818 T3 ES2123818 T3 ES 2123818T3 ES 94925314 T ES94925314 T ES 94925314T ES 94925314 T ES94925314 T ES 94925314T ES 2123818 T3 ES2123818 T3 ES 2123818T3
Authority
ES
Spain
Prior art keywords
assay
metaplastic
neutral
animal
removal
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired - Lifetime
Application number
ES94925314T
Other languages
English (en)
Inventor
Douglas J Pon
Louise Boulet
Staden Carlo J Van
Rejean Fortin
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Merck Frosst Canada and Co
Original Assignee
Merck Frosst Canada and Co
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Merck Frosst Canada and Co filed Critical Merck Frosst Canada and Co
Application granted granted Critical
Publication of ES2123818T3 publication Critical patent/ES2123818T3/es
Anticipated expiration legal-status Critical
Expired - Lifetime legal-status Critical Current

Links

Classifications

    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/5005—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving human or animal cells
    • G01N33/5008—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving human or animal cells for testing or evaluating the effect of chemical or biological compounds, e.g. drugs, cosmetics
    • G01N33/5014—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving human or animal cells for testing or evaluating the effect of chemical or biological compounds, e.g. drugs, cosmetics for testing toxicity
    • G01N33/5017—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving human or animal cells for testing or evaluating the effect of chemical or biological compounds, e.g. drugs, cosmetics for testing toxicity for testing neoplastic activity
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
    • Y10T—TECHNICAL SUBJECTS COVERED BY FORMER US CLASSIFICATION
    • Y10T436/00—Chemistry: analytical and immunological testing
    • Y10T436/25—Chemistry: analytical and immunological testing including sample preparation
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
    • Y10T—TECHNICAL SUBJECTS COVERED BY FORMER US CLASSIFICATION
    • Y10T436/00—Chemistry: analytical and immunological testing
    • Y10T436/25—Chemistry: analytical and immunological testing including sample preparation
    • Y10T436/25125—Digestion or removing interfering materials
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
    • Y10T—TECHNICAL SUBJECTS COVERED BY FORMER US CLASSIFICATION
    • Y10T436/00—Chemistry: analytical and immunological testing
    • Y10T436/25—Chemistry: analytical and immunological testing including sample preparation
    • Y10T436/25375—Liberation or purification of sample or separation of material from a sample [e.g., filtering, centrifuging, etc.]

Landscapes

  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Immunology (AREA)
  • Biomedical Technology (AREA)
  • Chemical & Material Sciences (AREA)
  • Hematology (AREA)
  • Toxicology (AREA)
  • Urology & Nephrology (AREA)
  • Molecular Biology (AREA)
  • Medicinal Chemistry (AREA)
  • Biochemistry (AREA)
  • Cell Biology (AREA)
  • Biotechnology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Food Science & Technology (AREA)
  • Tropical Medicine & Parasitology (AREA)
  • Physics & Mathematics (AREA)
  • Analytical Chemistry (AREA)
  • Microbiology (AREA)
  • General Health & Medical Sciences (AREA)
  • General Physics & Mathematics (AREA)
  • Pathology (AREA)
  • Investigating Or Analysing Biological Materials (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Cosmetics (AREA)
  • Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)
  • Acyclic And Carbocyclic Compounds In Medicinal Compositions (AREA)

Abstract

SE PRESENTA UN METODO PARA LA ESTIMACION RAPIDA DE LOS CAMBIOS HIPERPLASTICOS E HIPERTROFICOS EN LAS VIAS RESPIRATORIAS DE UN ANIMAL EN UN ENSAYO QUE MIDE ESPECIFICAMENTE LAS MUCOPROTEINAS ACIDICAS Y NEUTRAS DE FORMA LINEAL ENTRE 0.5 {MI}G Y AL MENOS 10 {MI}G. EL ENSAYO COMPRENDE LA EXPOSICION DE UN ANIMAL DE PRUEBA A UN INDUCTOR METAPLASTICO, LA SEPARACION DE LOS PULMONES, LA HOMOGENEIZACION EN UNA SOLUCION NEUTRALIZADORA APROPIADA QUE CONTENGA AGENTES REDUCTORES E INHIBIDORES DE PROTEASAS; LA SEPARACION DE LA MATERIA EN PARTICULAS; Y LA FRACCIONAMIENTO DEL EXTRACTO SOLUBLE TRATADO CON SDS. EL MATERIAL DE ALTO PESO MOLECULAR SE INMOVILIZA Y SE TIÑE PARA CUALQUIERA DE LAS MUCOSUBSTANCIAS ACIDICAS O NEUTRAS Y SE CUANTIFICA LA COLORACION ESPECIFICA. LOS CAMBIOS OBSERVADOS SON CONSISTENTES CON AQUELLOS VISTOS EN SECCIONES HISTOLOGICAS EN TEJIDOS EXPUESTOS. EL ENSAYO ES UTIL EN LA CONFIRMACION DEL POTENCIAL METAPLASTICO DE COMPUESTOS SOSPECHOSOS, EN LA DETERMINACION DE QUE MEDIADOR NEUROHUMORALESTA RELACIONADO CON LA METAPLASIA DE LAS CELULAS DEL MUCUS EN MODELOS ANIMALES PARA ENFERMEDADES PULMONARES OBSTRUCTIVAS CRONICAS, Y EN LA IDENTIFICACION DE COMPUESTOS QUE PUEDAN MEJORAR ESTOS EFECTOS.
ES94925314T 1993-09-13 1994-08-30 Procedimiento para medir las variaciones metaplasicas de los epitelocitos mucosecretores. Expired - Lifetime ES2123818T3 (es)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
US12071993A 1993-09-13 1993-09-13

Publications (1)

Publication Number Publication Date
ES2123818T3 true ES2123818T3 (es) 1999-01-16

Family

ID=22392139

Family Applications (1)

Application Number Title Priority Date Filing Date
ES94925314T Expired - Lifetime ES2123818T3 (es) 1993-09-13 1994-08-30 Procedimiento para medir las variaciones metaplasicas de los epitelocitos mucosecretores.

Country Status (9)

Country Link
US (1) US5932481A (es)
EP (1) EP0719412B1 (es)
JP (1) JP3436311B2 (es)
AT (1) ATE173087T1 (es)
CA (1) CA2171477C (es)
DE (1) DE69414405T2 (es)
DK (1) DK0719412T3 (es)
ES (1) ES2123818T3 (es)
WO (1) WO1995008114A1 (es)

Families Citing this family (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CA2285940A1 (en) * 1997-04-11 1998-10-22 K. Ramakrishnan Bhaskar Use of chondroitinase in the manufacture of a medicament in the treatment and prevention of mucoid secretions
US7354894B2 (en) 1998-08-18 2008-04-08 The Regents Of The University Of California Preventing airway mucus production by administration of EGF-R antagonists
CZ2001584A3 (cs) 1998-08-18 2002-06-12 The Regents Of The University Of California Prevence tvorby hlenu v dýchacích cestách podáváním antagonistů EGF-R
KR100753899B1 (ko) 2005-12-27 2007-08-31 재단법인서울대학교산학협력재단 알시안 블루를 이용한 시각화 방법
US9833207B2 (en) 2012-08-08 2017-12-05 William Harrison Zurn Analysis and clearing module, system and method
US9962533B2 (en) 2013-02-14 2018-05-08 William Harrison Zurn Module for treatment of medical conditions; system for making module and methods of making module

Family Cites Families (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4078082A (en) * 1974-11-13 1978-03-07 S.A. Joullie International Mucolytically effective agents
JP2517293B2 (ja) * 1987-06-25 1996-07-24 メクト株式会社 細胞、組織修復剤

Also Published As

Publication number Publication date
ATE173087T1 (de) 1998-11-15
JPH09502524A (ja) 1997-03-11
US5932481A (en) 1999-08-03
WO1995008114A1 (en) 1995-03-23
DE69414405T2 (de) 1999-06-10
DK0719412T3 (da) 1999-07-19
EP0719412A1 (en) 1996-07-03
DE69414405D1 (de) 1998-12-10
CA2171477C (en) 2004-10-19
JP3436311B2 (ja) 2003-08-11
CA2171477A1 (en) 1995-03-23
EP0719412B1 (en) 1998-11-04

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