ES2119779T3 - Solubilizacion de proteinas en formas activas. - Google Patents

Solubilizacion de proteinas en formas activas.

Info

Publication number
ES2119779T3
ES2119779T3 ES91915661T ES91915661T ES2119779T3 ES 2119779 T3 ES2119779 T3 ES 2119779T3 ES 91915661 T ES91915661 T ES 91915661T ES 91915661 T ES91915661 T ES 91915661T ES 2119779 T3 ES2119779 T3 ES 2119779T3
Authority
ES
Spain
Prior art keywords
protein
solubilization
proteins
active forms
refolding
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired - Lifetime
Application number
ES91915661T
Other languages
English (en)
Inventor
Nirdosh Kumar Puri
Enzo Crivelli
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Southern Cross Biotech Pty Ltd
Original Assignee
Southern Cross Biotech Pty Ltd
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Southern Cross Biotech Pty Ltd filed Critical Southern Cross Biotech Pty Ltd
Application granted granted Critical
Publication of ES2119779T3 publication Critical patent/ES2119779T3/es
Anticipated expiration legal-status Critical
Expired - Lifetime legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K1/00—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length
    • C07K1/107—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length by chemical modification of precursor peptides
    • C07K1/113—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length by chemical modification of precursor peptides without change of the primary structure
    • C07K1/1133—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length by chemical modification of precursor peptides without change of the primary structure by redox-reactions involving cystein/cystin side chains
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
    • C07K14/575—Hormones
    • C07K14/61—Growth hormone [GH], i.e. somatotropin

Landscapes

  • Chemical & Material Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Organic Chemistry (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Medicinal Chemistry (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Biochemistry (AREA)
  • Biophysics (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Endocrinology (AREA)
  • Molecular Biology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Analytical Chemistry (AREA)
  • General Chemical & Material Sciences (AREA)
  • Toxicology (AREA)
  • Zoology (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Peptides Or Proteins (AREA)
  • Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)

Abstract

UN METODO PARA LA PREPARACION DE UNA PROTEINA EN UNA FORMA FISIOLOGICAMENTE ACTIVA, CUYO METODO CONSISTE EN OBTENER DE UNA FUENTE DE PROTEINAS AL MENOS UNA FORMA PARCIALMENTE SOLUBILIZADA; Y UN AGENTE DE REPLEGADO QUE INCLUYE UNA CANTIDAD EFECTIVA DE UN MONOTIOL DE BAJO PESO MOLECULAR O UN DERIVADO DEL MISMO; SOMETER LA PROTEINA SOLUBILIZADA A UN PASO DE REPLEGADO, ANTE LA PRESENCIA DE UN AGENTE DE REPLEGADO.
ES91915661T 1990-09-05 1991-08-29 Solubilizacion de proteinas en formas activas. Expired - Lifetime ES2119779T3 (es)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
AUPK215990 1990-09-05

Publications (1)

Publication Number Publication Date
ES2119779T3 true ES2119779T3 (es) 1998-10-16

Family

ID=3774938

Family Applications (1)

Application Number Title Priority Date Filing Date
ES91915661T Expired - Lifetime ES2119779T3 (es) 1990-09-05 1991-08-29 Solubilizacion de proteinas en formas activas.

Country Status (8)

Country Link
EP (1) EP0547102B1 (es)
AT (1) ATE168115T1 (es)
AU (1) AU664021B2 (es)
CA (1) CA2090701C (es)
DE (1) DE69129747T2 (es)
DK (1) DK0547102T3 (es)
ES (1) ES2119779T3 (es)
WO (1) WO1992004382A1 (es)

Families Citing this family (11)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO1994001453A1 (en) * 1992-07-02 1994-01-20 Pitman-Moore, Inc. A process for recovering a recombinant protein, in biologically active form, from a solution containing inactive protein
DK44593D0 (da) * 1993-04-20 1993-04-20 Novo Nordisk As Fremgangsmaade til fremstilling af et polypeptid
WO1995008573A1 (fr) * 1993-09-22 1995-03-30 Ajinomoto Co., Inc. Peptide a action inhibant l'agregation plaquettaire et procede de production de ce compose
ZA955789B (en) * 1994-07-15 1996-03-11 Novo Nordisk As A method of converting a hydrophobic derivative of a polypeptide into the native form
DE10309691B4 (de) * 2003-02-28 2018-10-04 Hans-Knöll-Institut für Naturstoff-Forschung e.V. Gewinnung von löslichen Proteinen aus der Biomasse rekombinanter Mikroorganismen
MX2011013898A (es) 2009-06-22 2012-05-22 Amgen Inc Proteínas replegadas que usan un estado de oxido-reduccion quimicamente controlado.
EP2445924B2 (en) 2009-06-25 2023-12-13 Amgen Inc. Capture purification processes for proteins expressed in a non-mammalian system
US10065987B2 (en) 2013-02-12 2018-09-04 Bristol-Myers Squibb Company High pH protein refolding methods
EP2956468B1 (en) 2013-02-12 2020-06-10 Bristol-Myers Squibb Company Tangential flow filtration based protein refolding methods
WO2014155349A2 (en) 2013-03-29 2014-10-02 Dr. Reddy's Laboratories Refolding of proteins
WO2017040363A1 (en) * 2015-09-02 2017-03-09 Merck Sharp & Dohme Corp. A process for obtaining insulin with correctly formed disulfide bonds

Family Cites Families (16)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JPS6079000A (ja) * 1983-10-04 1985-05-04 Takeda Chem Ind Ltd ヒトγ型インタ−フエロン単量体の製造法
ATE92935T1 (de) * 1984-12-06 1993-08-15 Synergen Biolog Inc Serinproteaseinhibitoren und isolierungsverfahren dazu.
DE3537708A1 (de) * 1985-10-23 1987-04-23 Boehringer Mannheim Gmbh Verfahren zur aktivierung von t-pa nach expression in prokaryonten
US4766205A (en) * 1985-11-13 1988-08-23 Beatrice Companies, Inc. Method for isolation of recombinant polypeptides in biologically active forms
DE3611817A1 (de) * 1986-04-08 1987-10-15 Boehringer Mannheim Gmbh Verfahren zur renaturierung von proteinen
AU612133B2 (en) * 1987-02-20 1991-07-04 Natinco Nv Production of proteins in active forms
IL86090A (en) * 1987-04-16 1993-03-15 Cetus Oncology Corp Production of purified, biologically active, bacterially produced recombinant human csf-1
EP0295859B1 (en) * 1987-06-15 1994-11-17 Southern Cross Biotech Pty.Ltd. Production of proteins in active forms
EP0301835A1 (en) * 1987-07-29 1989-02-01 Schering Biotech Corporation Purification of human interleukin-4 expressed in Escherichia Coli
CA1340586C (en) * 1988-09-23 1999-06-08 Cetus Corporation Process for recovering microbially produced interferon-beta
DE3832898A1 (de) * 1988-09-28 1990-04-12 Boehringer Mannheim Gmbh Praeparat von in prokaryonten exprimiertem plasminogenaktivator
DE3901719A1 (de) * 1989-01-21 1990-07-26 Hoechst Ag Verfahren zur herstellung eines insulinvorlaeufers
DE3901718A1 (de) * 1989-01-21 1990-07-26 Hoechst Ag Verfahren zur renaturierung falscher rekombinanten von insulinvorlaeufern
GB8927546D0 (en) * 1989-12-06 1990-02-07 Ciba Geigy Process for the production of biologically active tgf-beta
US5235043A (en) * 1990-04-06 1993-08-10 Synergen, Inc. Production of biologically active, recombinant members of the ngf/bdnf family of neurotrophic proteins
DE4113750A1 (de) * 1991-04-26 1992-10-29 Boehringer Mannheim Gmbh Verbesserung der renaturierung bei der sekretion von disulfidverbrueckten proteinen

Also Published As

Publication number Publication date
DE69129747T2 (de) 1998-11-12
CA2090701C (en) 2000-05-16
EP0547102A4 (es) 1994-04-27
DE69129747D1 (de) 1998-08-13
DK0547102T3 (da) 1999-04-19
AU664021B2 (en) 1995-11-02
CA2090701A1 (en) 1992-03-10
EP0547102B1 (en) 1998-07-08
ATE168115T1 (de) 1998-07-15
EP0547102A1 (en) 1993-06-23
WO1992004382A1 (en) 1992-03-19
AU8403591A (en) 1992-03-30

Similar Documents

Publication Publication Date Title
ES2065911T3 (es) Produccion de proteinas en formas activas.
DK0538300T3 (da) O-Glycosyleret IFN-alfa
DE69524864D1 (de) 4-amino-derivate der mycophenolsäure mit immun suppressiver wirkung
IS3824A (is) Protein með bundið polyethylen og aðferð til framleiðslu þess
ATE210127T1 (de) 6-substituierte mycophenolsäurederivate mit immun suppressiver wirkung
MX9102353A (es) N,o-sulfados de heparosanos, su procedimiento de preparacion y las composiciones farmaceuticas que contienen.
MXPA05008409A (es) Formulacion de inmunoglobulina y metodo para la preparacion de la misma.
DE69708121D1 (de) Halogenid-enthaltende insulinzubereitungen
ES2119779T3 (es) Solubilizacion de proteinas en formas activas.
ATE114319T1 (de) Produktion von proteinen in aktiven formen.
DE69101634T2 (de) Rekombinant hergestellte blutfaktoren, verfahren zur exprimierung besagter blutfaktoren sowie in besagtem prozess verwendeter rekombinanter vaccina-virus.
IT9022341A0 (it) Estratti da tessuti animali, utili in terapia e in diagnostica.
SE8700156D0 (sv) Kosmetiska kompositioner med hudlugnande och hudgenerande effekt och forfarande for framstellning derav
ATE210126T1 (de) 4-amino-6-substituierte mycophenolsäurederivate mit immunsuppressiver wirkung
NO943968D0 (no) Nytt anti-neoplastisk cytokin
BR9104547A (pt) Processo de curtimento de uma substancia proteica,substancia proteica curtida e agente de curtimento de proteinas
FR2623378B1 (fr) Gaine de maintien abdominale

Legal Events

Date Code Title Description
FG2A Definitive protection

Ref document number: 547102

Country of ref document: ES