ES2118049A1 - Carbamate kinase: its preparation and use - Google Patents
Carbamate kinase: its preparation and useInfo
- Publication number
- ES2118049A1 ES2118049A1 ES09602525A ES9602525A ES2118049A1 ES 2118049 A1 ES2118049 A1 ES 2118049A1 ES 09602525 A ES09602525 A ES 09602525A ES 9602525 A ES9602525 A ES 9602525A ES 2118049 A1 ES2118049 A1 ES 2118049A1
- Authority
- ES
- Spain
- Prior art keywords
- carbamate
- carbamate kinase
- kinase
- preparation
- adp
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Granted
Links
Landscapes
- Enzymes And Modification Thereof (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
Abstract
Carbamate kinase: its preparation and use. The carbamate kinase of Enterococcus (formerly Streptococcus) faecalis which catalyses, in the presence of mg2+, the formation of carbamate and adenosine triphosphate (ATP) from carbamyl phosphate and adenosine diphosphate (ADP) and the reverse reaction from carbamate and ATP to carbamyl phosphate and ADP, is prepared by genetic engineering techniques. A gene coding for the carbamate kinase of the usual source of the enzyme (Enterococcus faecalis) is isolated, sequenced and used to transform a micro-organism and generate a transformant which can be cultured to produce carbamate kinase in high yield. A purification process which can be used to produce essentially pure enzyme is used.
Priority Applications (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
ES9602525A ES2118049B1 (en) | 1996-11-29 | 1996-11-29 | CARBAMATE KINASE: ITS PREPARATION AND USE. |
Applications Claiming Priority (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
ES9602525A ES2118049B1 (en) | 1996-11-29 | 1996-11-29 | CARBAMATE KINASE: ITS PREPARATION AND USE. |
Publications (2)
Publication Number | Publication Date |
---|---|
ES2118049A1 true ES2118049A1 (en) | 1998-09-01 |
ES2118049B1 ES2118049B1 (en) | 1999-04-01 |
Family
ID=8296856
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
ES9602525A Expired - Lifetime ES2118049B1 (en) | 1996-11-29 | 1996-11-29 | CARBAMATE KINASE: ITS PREPARATION AND USE. |
Country Status (1)
Country | Link |
---|---|
ES (1) | ES2118049B1 (en) |
-
1996
- 1996-11-29 ES ES9602525A patent/ES2118049B1/en not_active Expired - Lifetime
Non-Patent Citations (4)
Title |
---|
BURNE, R.A., et al., "Cloning and expression in Escherichia coli of the arginine deiminase system of Streptococcus sanguis NCTC 10904", INFECTION AND IMMUNITY, 1989, vol. 57, no. 11, pßgs. 3540-3548. *Ver el documento completo* * |
MARINA, A. et al., "Crystallization, characterization and preliminary crystallographic studies of carbamate kinase of Streptococcus faecium", JOURNAL OF MOLECULAR BIOLOGY, 1994, vol. 235, pßgs. 1345-1347. *Ver el documento completo* * |
SIMON, J.P., et al., "Control of enzyme synthesis in the arginine deiminase pathway of Streptococcus faecalis", JOURNAL OF BACTERIOLOGY, 1982, vol. 150, no. 3, pßgs. 1085-1090. *Ver el documento completo* * |
SIMON, J.P., et al., "Enzymes of agmatine degradation and the control of their synthesis in Streptococcus faecalis", JOURNAL OF BACTERIOLOGY, 1982, vol. 152, no. 2, pßgs. 676-681. *Ver el documento completo* * |
Also Published As
Publication number | Publication date |
---|---|
ES2118049B1 (en) | 1999-04-01 |
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Legal Events
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