ES2114504B1 - Metodo para la sintesis enzimatica de desoxioligonucleotidos en soporte solido. - Google Patents
Metodo para la sintesis enzimatica de desoxioligonucleotidos en soporte solido.Info
- Publication number
- ES2114504B1 ES2114504B1 ES9601945A ES9601945A ES2114504B1 ES 2114504 B1 ES2114504 B1 ES 2114504B1 ES 9601945 A ES9601945 A ES 9601945A ES 9601945 A ES9601945 A ES 9601945A ES 2114504 B1 ES2114504 B1 ES 2114504B1
- Authority
- ES
- Spain
- Prior art keywords
- solid support
- deoxyoligonucleotides
- enzymatic synthesis
- synthesis
- transferase
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Expired - Fee Related
Links
Landscapes
- Preparation Of Compounds By Using Micro-Organisms (AREA)
- Enzymes And Modification Thereof (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
Abstract
METODO PARA LA SINTESIS ENZIMATICA DE DESOXIOLIGONUCLEOTIDOS EN SOPORTE SOLIDO, QUE CONSISTE EN REALIZAR LA SINTESIS SOBRE UN SOPORTE SOLIDO EN LA DIRECCION (5'') A (3''), UTILIZANDO LA TRANSFERASA TERMINAL, UNA ADN POLIMERASA INDEPENDIENTE DE MOLDE QUE PREFERENTEMENTE, CATALIZA LA INCORPORACION DE DESOXIRRIBONUCLEOSIDOS 5" TRIFOSFATOS A TERMINALES 3", HIDROXILO DE ADN MONOCATENARIO Y SE EMPLEA DESOXIRRIBONUCLEOTIDOS CON SU HIDROXILO (3'') TRANSITORIAMENTE PROTEGIDO CON OBJETO DE AÑADIR UN SOLO NUCLEOTIDO EN CADA CICLO.
Priority Applications (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
ES9601945A ES2114504B1 (es) | 1996-09-13 | 1996-09-13 | Metodo para la sintesis enzimatica de desoxioligonucleotidos en soporte solido. |
Applications Claiming Priority (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
ES9601945A ES2114504B1 (es) | 1996-09-13 | 1996-09-13 | Metodo para la sintesis enzimatica de desoxioligonucleotidos en soporte solido. |
Publications (2)
Publication Number | Publication Date |
---|---|
ES2114504A1 ES2114504A1 (es) | 1998-05-16 |
ES2114504B1 true ES2114504B1 (es) | 1999-02-01 |
Family
ID=8296028
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
ES9601945A Expired - Fee Related ES2114504B1 (es) | 1996-09-13 | 1996-09-13 | Metodo para la sintesis enzimatica de desoxioligonucleotidos en soporte solido. |
Country Status (1)
Country | Link |
---|---|
ES (1) | ES2114504B1 (es) |
Family Cites Families (5)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
DE3301833A1 (de) * | 1983-01-20 | 1984-07-26 | Gesellschaft für Biotechnologische Forschung mbH (GBF), 3300 Braunschweig | Verfahren zur simultanen synthese mehrerer oligonocleotide an fester phase |
US4605735A (en) * | 1983-02-14 | 1986-08-12 | Wakunaga Seiyaku Kabushiki Kaisha | Oligonucleotide derivatives |
US4816571A (en) * | 1987-06-04 | 1989-03-28 | Applied Biosystems, Inc. | Chemical capping by phosphitylation during oligonucleotide synthesis |
SE9003743D0 (sv) * | 1990-11-26 | 1990-11-26 | Pharmacia Ab | Method and means for oligonucleotide synthesis |
US5872244A (en) * | 1994-09-02 | 1999-02-16 | Andrew C. Hiatt | 3' protected nucleotides for enzyme catalyzed template-independent creation of phosphodiester bonds |
-
1996
- 1996-09-13 ES ES9601945A patent/ES2114504B1/es not_active Expired - Fee Related
Also Published As
Publication number | Publication date |
---|---|
ES2114504A1 (es) | 1998-05-16 |
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Legal Events
Date | Code | Title | Description |
---|---|---|---|
EC2A | Search report published |
Date of ref document: 19980516 Kind code of ref document: A1 Effective date: 19980516 |
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FD2A | Announcement of lapse in spain |
Effective date: 20161227 |